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    Wyświetlanie 1-3 z 3
    Tytuł:
    The angiotensin converting enzyme gene i/d polymorphism in Polish rowers
    Autorzy:
    Jastrzębski, Zbigniew
    Leońska-Duniec, Agata
    Kolbowicz, Marek
    Tomiak, Tomasz
    Powiązania:
    https://bibliotekanauki.pl/articles/1055139.pdf
    Data publikacji:
    2014
    Wydawca:
    Uniwersytet Szczeciński. Wydawnictwo Naukowe Uniwersytetu Szczecińskiego
    Tematy:
    ACE I/D polymorphism
    athlete status
    rowers
    sport genetics
    Opis:
    Angiotensin converting enzyme gene (ACE) is the most frequently investigated genetic marker in the context of genetic conditioning of athletic predispositions. The product of the gene is a key component of the renin-angiotensin system (RAS) and the kallikrein-kinin system (KKS), mainly responsible for the regulation of blood pressure. The main aim of the study was to determine the possible interaction between the ACE I/D polymorphism and endurance athlete status in a group of Polish rowers in comparison with sedentary individuals. 121 male Polish rowers, members of academic sports clubs, and 115 unrelated volunteers, were recruited for the study. The PCR amplification of the insertion (I) or deletion (D) fragment of the ACE gene was performed. Compared with control group, the frequency of the I allele differ significantly from that found in rowers (57.4% vs. 44.3%; P=0.013) and the ACE genotype frequency amongst the whole cohort of rowers (30.6% II, 53.7% ID, 15.7% DD) was also different from expected values (control group 19.1% II, 50.4% ID, 30.4% DD; P=0.017). Our investigation confirms a positive association of the I allele of the ACE gene with endurance athlete status in a group of Polish rowers.
    Źródło:
    Central European Journal of Sport Sciences and Medicine; 2014, 5, 1; 77-82
    2300-9705
    2353-2807
    Pojawia się w:
    Central European Journal of Sport Sciences and Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Molecular Identification of Free-living Amoebae Isolated from Artificial Water Bodies Located in Poland
    Autorzy:
    LEOŃSKA-DUNIEC, Agata
    SKOTARCZAK, Bogumiła
    ADAMSKA, Małgorzata
    Powiązania:
    https://bibliotekanauki.pl/articles/763628.pdf
    Data publikacji:
    2015
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Artificial water samples, free living amoebae, thermal tolerance test, PCR, DNA sequencing, Acanthamoeba, Vermoamoeba
    Opis:
    Free living amoebae (FLA) are amphizoic protozoa that are widely found in various environmental sources. They are known to cause serious human infections, including a fatal encephalitis, a blinding keratitis, and pneumonia. The main aim of the study was detection and molecular identification of Acanthamoeba spp., Naegleria fowleri, Balamuthia mandrillaris, Sappinia pedata, and Vermoamoeba vermiformis (formerly Hartmannella vermiformis) in artificial water bodies in North-Western Poland. We examined 86 water samples collected during 2-year period from 43 water bodies, including outdoor and indoor swimming pools, firefighting reservoirs, fountains, as well as water network. The samples were filtrated using Filta-Max® membrane filters (IDEXX Laboratories, USA) and, in order to select potentially pathogenic, thermophilic strains and to limit the number of PCR examined samples, the thermal tolerance test was carried out. Obtained filtrates were transferred to non-nutrient agar plates with E. coli. The agar plates were incubated at 37°C and then proliferated amoebae were passaged at 42°C. DNA was extracted from the thermophilic trophozoites and then polymerase chain reactions and sequence analysis were performed for molecular identification of FLA. From the 86 collected water samples 57 strains of FLA were able to proliferate at 37°C and 7 of them showed ability to proliferate at 42°C. For molecular identification of Acanthamoeba spp. and V. vermiformis, regions of 18S rDNA were amplified. In order to detect B. mandrillaris DNA, we used mitochondrial 16S rDNA as a marker, and for detection of N. fowleri and S. pedata – ITS regions. Based on molecular analysis, isolates were classified to the genus Acanthamoeba (T4 and T11 genotypes, as well as the new genotypes detected earlier in clinical samples and named T16) and V. vermiformis species. Detected strains were highly similar or identical to pathogenic strains detected earlier in patients. Our results show a wide distribution of potential pathogenic FLA, as Acanthamoeba T4, T11, T16 genotypes, and V. vermiformis species in various artificial water bodies located in North-Western Poland and suggest a potential threat to health of humans in this part of the country.
    Źródło:
    Acta Protozoologica; 2015, 54, 1
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic research in modern sport
    Autorzy:
    Leońska-Duniec, Agata
    Powiązania:
    https://bibliotekanauki.pl/articles/1054958.pdf
    Data publikacji:
    2013
    Wydawca:
    Uniwersytet Szczeciński. Wydawnictwo Naukowe Uniwersytetu Szczecińskiego
    Tematy:
    athlete status
    physical activity
    polymorphism
    sport genetics
    Opis:
    Sport genomics is a comparatively new scientific discipline concentrating on the organization and functioning of the genome of elite athletes. It seems to be the most promising tool for sport selection, individualization of the training process, sport traumatology, and also in illegal ‘gene doping’. With genotyping more available, research of gene variants’ influence on several phenotype traits related to physical performance have been widely carried out worldwide. This review not only summarizes the current findings of sport genomics study of molecular markers, their association with athlete status and training responses, but it also explores future trends and possibilities. The importance of genetics in modern sport increases every year. However, the recent studies still represent only the first steps towards a better understanding of the genetic factors that influence human physical abilities, and therefore continuing studies are necessary.
    Źródło:
    Central European Journal of Sport Sciences and Medicine; 2013, 3, 3; 19-26
    2300-9705
    2353-2807
    Pojawia się w:
    Central European Journal of Sport Sciences and Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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