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Tytuł:
Effect of magnetite composite on the amount of double strand breaks induced with X-rays
Autorzy:
Wiecheć, A.
Nowicka, K.
Błażewicz, M.
Kwiatek, W.
Powiązania:
https://bibliotekanauki.pl/articles/1065182.pdf
Data publikacji:
2016-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.50.-a
87.53.-j
81.05.Mh
81.05.Ni
81.05.Pj
81.05.Qk
75.50.Bb
Opis:
The aim of this study was to find out if polylactide (PLA) modified with magnetite might affect the amount of DNA double strand breaks induced with X-rays. The human osteosarcoma cells (MG63) were seeded on the polystyrene cell culture dishes (PS), PLA and PLA modified with magnetite substrates. The double strand breaks were analyzed after X-ray irradiation (dose rate 2 Gy/min), in the first day of culturing. The number of double strand breaks increased in the PLA modified with magnetite, for example after 1 Gy of X-rays irradiation, double strand breaks/cell equaled: 24.5 vs. 17.5 and 17.3, for PLA modified with magnetite vs. PLA and PS, p < 0.0003. We conclude that PLA modified with magnetite changed the number of double strand breaks induced with X-rays. However, more research is needed to confirm that such composite might be considered as radiosensitizer in radiotherapy.
Źródło:
Acta Physica Polonica A; 2016, 129, 2; 174-175
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Study of Human Osteoblast-Like MG 63 Cells Proliferation on Resorbable Polymer-Based Nanocomposites Modified with Ceramic and Carbon Nanoparticles
Autorzy:
Wiecheć, A.
Stodolak-Zych, E.
Frączek-Szczypta, A.
Błażewicz, M.
Kwiatek, W.
Powiązania:
https://bibliotekanauki.pl/articles/1490257.pdf
Data publikacji:
2012-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
82.35.Np
87.17.Ee
Opis:
Polymer-based nanocomposites containing biocompatible and bioactive nanocomponents seem to be excellent materials that could be used in many biomedical applications. The aim of this study was biological evaluation of resorbable polymer-based nanocomposites (PLA, PCL) and their modifications with ceramic nanoparticles (silica - $SiO_2$, montmorillonite - MMT) or carbon nanotubes. The nanocomposites were seeded with the human osteoblast-like MG 63 cells. After 1, 3 and 7 days of incubation, Trypan blue exclusion test was used to determine the viability and number of cells. The cell population density depending on incubation time and cell population doubling time was calculated. The cell proliferation abilities on the all applied nanocomposites and on control material (polystyrene cell culture plate) were also compared. The number of cells growing on the nanocomposite surfaces increased with the incubation time. The cell viability was not decreased for all applied materials during the entire study (97-100%). The ceramic nanoparticles and carbon nanotubes modified the bone cell growth and proliferation rate. Results of this study confirm that all types of the nanocomposites are appropriate to the growing and proliferation of human osteoblast-like cells.
Źródło:
Acta Physica Polonica A; 2012, 121, 2; 546-550
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rozkład wybranych metali ciężkich w osadach dennych Zbiornika Dobczyckiego
Distribution of heavy metals in sediments of Dobczyce reservoir
Autorzy:
Reczynski, W.
Kwiatek, W.M.
Kubica, B.
Golas, J.
Jakubowska, M.
Niewiara, E.
Dutkiewicz, E.
Stobinski, M.
Skiba, M.
Powiązania:
https://bibliotekanauki.pl/articles/15167.pdf
Data publikacji:
2006
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie / Polskie Towarzystwo Magnezologiczne im. Prof. Juliana Aleksandrowicza
Tematy:
zbiornik Dobczyce
osady denne
metale ciezkie
pojemnosc sorpcyjna
stezenie pierwiastkow
arsen
kadm
chrom
miedz
Opis:
Osady denne stanowią istotny składnik środowiska wodnego, akumulując składniki mineralne, w tym metale ciężkie. Zbiornik Dobczycki, będąc rezerwuarem wody pitnej dla aglomeracji krakowskiej, spełnia bardzo ważną funkcję gospodarczą. W badaniach osadów dennych Zbiornika Dobczyckiego wykonano analizę wierzchniej ich warstwy w 17 punktach pomiarowych. Pozwoliło to na opisanie dystrybucji metali w czaszy zbiornika oraz ocenę czynników warunkujących tę dystrybucję. Analizę wykonano dwoma metodami: metodą atomowej spektrometrii absorpcyjnej oraz metodą PIXE. Stwierdzono, z wykorzystaniem narzędzi statystycznych, że ze względu na stężenie metali, punkty poboru próbek można podzielić na dwie grupy: leżące poza dawnym nurtem rzeki Raby (niższe stężenia metali ciężkich) oraz usytuowane w dawnym nurcie rzeki (wyższe stężenia). Odpowiada to zmieniającym się właściwościom sorpcyjnym osadów, zależnym m.in. od uziarnienia, składu chemicznego i mineralnego oraz zawartości materii organicznej. Zastosowanie metody PIXE umożliwiło wykonanie wielopierwiastkowej analizy składu bez wstępnej procedury przygotowania próbek, z wystarczającą czułością oznaczeń, lecz mniejszą precyzją niż metoda ASA. Metody te mogą być komplementarne z punktu widzenia potrzeb analiz środowiskowych.
Sediments constitute important part of water systems, accumulating mineral compounds, such as heavy metals. Dobczyce Reservoir, being the main drinking water source for the city of Krakow, is important from the economical point of view. In the present research, analysis of elemental composition of sediment samples (upper 10 cm layer) in 17 sampling points was performed. It enabled us to analyse distribution of analysis of elements in Dobczyce Reservoir and to assess what factors influenced that distribution. The analysis was carried out by means of two instrumental methods, namely PIXE and ASA. Based on the statistical analysis of the results, it was found that all the sampling points could be divided into two groups – one situated out of the Raba river current (characterized by lower concentration of elements) and the other along the river current (higher concentration of elements). Such a division results from the differences in chemical and mineralogical composition of sediments as well as from their organic matter content and physical properties. The use of PIXE method makes it possible to analyze sediments in a multielemental mode without sample pretreatment, obtaining satisfactory sensitivity but less precision than with the use of AAS method. Both methods could be considered as complementary in environmental analysis.
Źródło:
Journal of Elementology; 2006, 11, 3
1644-2296
Pojawia się w:
Journal of Elementology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of Human Lenses by Raman Microspectroscopy
Autorzy:
Paluszkiewicz, C.
Chaniecki, P.
Rękas, M.
Rajchel, B.
Piergies, N.
Kwiatek, W.
Powiązania:
https://bibliotekanauki.pl/articles/1398828.pdf
Data publikacji:
2016-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.kp
42.55.Ye
87.64.-t
Opis:
A cataract is an opacity (clouding) of the normally clear lens which develops as a result of aging, metabolic disorders, trauma or heredity. The number of patients with cataract is increasing exponentially. This disease requires surgical intervention, to remove the cloudy lens and to introduce the eye lens polymer. In this work we will present analyses of degraded parts of human lens. Experimental materials were obtained from the lens removed during surgical intervention. These biological samples were measured using Almega XR Confocal Raman spectrometer (Thermo Scientific) with an excitation source of 785 nm laser line. The Raman vibrations in the spectral region of 650-1750 cm¯¹ were analyzed. The difference spectra revealed an excess of tryptophan, tyrosine, phenylalanine, β-sheet conformation, and molecules or molecular groups.
Źródło:
Acta Physica Polonica A; 2016, 129, 2; 244-246
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Surface Study of Selected Biomaterials Using Vibrational Spectroscopy
Autorzy:
Paluszkiewicz, C.
Kwiatek, W.
Długoń, E.
Wesełucha-Birczyńska, A.
Piccinini, M.
Powiązania:
https://bibliotekanauki.pl/articles/1809387.pdf
Data publikacji:
2009-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.km
87.64.kp
87.64.Ee
87.85.jf
Opis:
Vibrational spectroscopy has been extensively used for in vitro and in vivo investigations of degradation mechanism and kinetics of different biomedical materials as well as it has been used to characterize the crystalline and amorphous domains in bio-mineralization process. Infrared and Raman spectroscopy methods are valuable tools in the biomaterials engineering allowing to study processes occurring during their preparation. In vitro tests, where the materials are immersed in simulated body fluids and/or artificial saliva, were used to evaluate the biocompatibility of biomaterials. This kind of tests are a wide range of repeatable and reproducible methods, which are regulated by international standards for commercial use and scientific development of new materials and products. The aim of this work was to examine phase composition of materials applied in dentistry. The bioactivity of such biomaterials was studied by immersing the samples in synthetic body fluid and artificial saliva. The changes were determined by the Fourier transform infrared and Raman microspectroscopy as well as scanning electron microscopy. It was found that results obtained by vibrational spectroscopy show the differences between the studied samples. Chemical reactions occurring during incubation of cements in artificial saliva as well as in synthetic body fluid result in formation of phosphates which deposit on the cement surface.
Źródło:
Acta Physica Polonica A; 2009, 115, 2; 533-536
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Infrared Spectroscopy in Molecular Study of the Piezoelectric Effect in Pigs Shin Bone
Autorzy:
Lipiec, E.
Kowalska, J.
Wiecheć, A.
Zieliński, P.
Kwiatek, W.
Iwaniec, M.
Powiązania:
https://bibliotekanauki.pl/articles/1490248.pdf
Data publikacji:
2012-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
78.30.Jw
07.57.Ty
77.65.-j
87.50.ct
Opis:
Despite growing number of literature demonstrating the piezoelectric effect in bones at macro level there's still a lack of papers describing this effect at molecular level. In order to examine this effect more specifically, a study has been conducted to show the possibility of using infrared spectroscopy on samples contained in the electric field. This is the first known research on applying infrared spectroscopy to study the influence of electric field and compressive stress on bones' structure represented by the molecular IR spectrum. The samples used in this experiment as a model were prepared in pellet form, made from powdered pig's shin bone mixed with KBr (mass ratio of about 1:100). The spectra were obtained in transmission mode. Three different types of experiments were performed at each sample, which were subjected to: (a) fixed electric field (E < 40 kV/m), (b) variable electric field (0-40 kV/m), (c) compressive stress (P < 100 MPa). The obtained data have shown that the electric field and compressive stress on sample modified infrared spectra of the bone. Qualitative as well as quantitative changes in the spectral range between 900 $cm^{-1}$ and 1200 $cm^{-1}$ and the band at 562 $cm^{-1}$ ($PO_4^{3-}$ asymmetric and symmetric stretching, respectively) were observed. The relative area obtained by decomposition of the $PO_4^{3-}$ band reaches an extreme value in the external compressive stress (10 MPa), that occur under physiological conditions. Based on presented data, it was proved that the applied factors (electric field, compressive stress) can change the oscillation energy and the number of molecule's degrees of freedom.
Źródło:
Acta Physica Polonica A; 2012, 121, 2; 539-542
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biomedical Applications of Synchrotron X-Ray Fluorescence
Autorzy:
Kwiatek, W. M.
Powiązania:
https://bibliotekanauki.pl/articles/1931695.pdf
Data publikacji:
1994-11
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
07.85.+n
87.59.-e
32.30.Rj
Opis:
Since the exact role of trace elements in living organisms is still not very well known, it is a need of a careful analysis and study on trace element distributions in various biomedical materials. The conventional techniques such as X-ray fluorescence, proton-induced X-ray emission, atomic absorption spectrophotometry do not always give precise determination, especially in microstructures areas. Since synchrotron radiation is very powerful and gives the opportunity to work with micro-beams the synchrotron radiation induced X-ray emission technique has been recently developed. Due to many properties of synchrotron radiation it becomes a very useful tool in science. In this paper a characterization of different apparatus for a trace element analysis as well as several applications are described to show the usefulness of the synchrotron radiation induced X-ray emission.
Źródło:
Acta Physica Polonica A; 1994, 86, 5; 695-703
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Distinguishing Prostate Cancer from Hyperplasia
Autorzy:
Kwiatek, W. M.
Banaś, A.
Banaś, K.
Podgórczyk, M.
Dyduch, G.
Falkenberg, G.
Gajda, M.
Cichocki, T.
Powiązania:
https://bibliotekanauki.pl/articles/2044731.pdf
Data publikacji:
2006-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.-t
Opis:
The prostate gland is the most common site of neoplastic disorders in men. The knowledge of the pathogenesis of benign prostate hyperplasia and prostate cancer is still under investigation. X-ray fluorescence analysis using synchrotron radiation seems to be an appropriate technique to study the elemental composition of biological tissues. The measurements presented in this paper were carried out on the L-beam line at the HASYLAB, DESY (Germany). The observed concentrations of Fe, Cu, Mn, and Zn are significantly higher in cancerous tissues, as compared to normal and hyperplastic ones. Our results reveal also the existence of the two types of hyperplasia.
Źródło:
Acta Physica Polonica A; 2006, 109, 3; 377-381
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Preliminary Study on Chemical Speciation of Sulphur in Cancerous Tissues
Autorzy:
Kwiatek, W. M.
Banaś, A.
Banaś, K.
Kisiel, A.
Cinque, G.
Falkenberg, G.
Powiązania:
https://bibliotekanauki.pl/articles/2044744.pdf
Data publikacji:
2006-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.Gb
Opis:
Sulphur is vital for almost all living organisms by participating in a wide variety of metabolic processes. Nevertheless its biochemistry is only partially understood, due to it a few tools such as X-ray absorption near edge structure may be used to determine its chemical speciation in biological system. In this paper we focused on analysis of composition and elemental distribution in tissue structures of biological samples. We examined cancerous and non-cancerous prostate tissues. Experiments were carried out on the L-beam line at the HASYLAB, DESY (Hamburg, Germany) and DAΦNE-Light Facility at the Laboratori Nationali di Frascati (Frascati, Italy). Synchrotron radiation induced X-ray emission measurements reveal the significant differences in concentration of sulphur between cancerous and non-cancerous tissues. X-ray absorption near edge structure spectra show two main types of sulphur that are represented by X-ray peaks at 2476 eV and 2484 eV. This suggests that different sulphur components are present in the measured samples.
Źródło:
Acta Physica Polonica A; 2006, 109, 3; 383-387
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of μ-FTIR-SR Spectroscopy to Prostate Tissue Analysis
Autorzy:
Kwiatek, W.
Paluszkiewicz, C.
Banaś, A.
Kisiel, A.
Podgórczyk, M.
Marcelli, A.
Cestelli Guidi, M.
Piccinini, M.
Powiązania:
https://bibliotekanauki.pl/articles/1810277.pdf
Data publikacji:
2009-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.km
87.64.kp
87.64.Ee
87.85.jf
Opis:
The infrared spectromicroscopy is a quite recent technique rapidly developing thanks to the availability of new instruments and new brilliant synchrotron radiation sources in different areas and in particular to biomedical researches. In order to achieve a diffraction limited spatial resolution in tissue samples, we performed experiments at SINBAD, the synchrotron infrared beamline of the Laboratori Nazionali di Frascati. We characterized the chemical composition of prostate tissue samples taken from patients affected by prostate cancer disease. Different sizes of the pinholes were considered for the measurements. In the case of prostate tissue sections the results show the possibility to determine the intensity ratio of the $CH_2$ and $CH_3$ bands set at 2930 $cm^{-1}$ and 2960 $cm^{-1}$, respectively. Experiments were also performed with a pinhole of 5 μm of diameter and the differences in both histological and chemical compositions of such samples were determined.
Źródło:
Acta Physica Polonica A; 2009, 115, 2; 602-605
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Sulphur XANES Analysis of Cultured Human Prostate Cancer Cells
Autorzy:
Kwiatek, W.
Podgórczyk, M.
Paluszkiewicz, Cz.
Balerna, A.
Kisiel, A.
Powiązania:
https://bibliotekanauki.pl/articles/1812260.pdf
Data publikacji:
2008-08
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.kd
87.19.xj
Opis:
Prostate cancer is one of the most commonly diagnosed cancers in men throughout the world. It is believed that changes to the structure of protein binding sites, altering its metabolism, may play an important role in carcinogenesis. Sulphur, often present in binding sites, can influence such changes through its chemical speciation. Hence there is a need for precise investigation of coordination environment of sulphur. X-ray absorption near edge structure spectroscopy offers such possibility. Cell culture samples offer histologically well defined areas of good homogeneity, suitable for successful and reliable X-ray absorption near edge structure analysis. This paper presents sulphur speciation data collected from three different human prostate cancer cell lines (PC-3, LNCaP and DU-145). Sulphur X-ray absorption near edge structure analysis was performed on K-edge structure. The spectra of cells were compared with those of cancerous tissue and with organic substances as well as inorganic compounds.
Źródło:
Acta Physica Polonica A; 2008, 114, 2; 463-470
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chemical Composition of Atherosclerotic Plaques οf apoE/LDLR-Double Knockout Mice by Synchrotron Radiation FTIR Microspectroscopy
Autorzy:
Kowalska, J.
Gajda, M.
Kwiatek, W.
Franczyk-Żarów, M.
Kostogrys, R.
Chłopicki, S.
Sandt, C.
Dumas, P.
Powiązania:
https://bibliotekanauki.pl/articles/1490259.pdf
Data publikacji:
2012-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.19.xr
87.64.km
87.64.-t
Opis:
Atherosclerosis is a multietiological inflammatory disease of large and medium-sized arteries of increasing incidence in westernized countries. The aim of this study was to identify the biochemical changes during the progression of atherosclerosis by synchrotron radiation Fourier transform infrared microspectroscopy in atheromas of apoE/$LDLR^{-//-}$ mice fed egg-rich diet supplemented or not with angiotensin converting enzyme inhibitor perindopril. Synchrotron radiation Fourier transform infrared microspectroscopy technique was used to obtain information at high spatial resolution about the distribution of proteins (C-N, N-H, CO for amide I and amide II bands), lipids ($CH_2$, $CH_3$ bands) as well as mineral deposits (calcium carbonates and phosphates). Total contents of lipids and proteins were found to be significantly lower in animals treated with the diet and perindopril. An increase in saturation level of lipids was observed in animals fed with egg-rich diet when compared to the normal diet and perindopril treatment, which did not inhibit this effect. Moreover, a significant change in the secondary structure of proteins (ratio between absorption bands 1634 $cm^{-1}$/1656 $cm^{-1}$ attributed to β-type and α-type, respectively) was observed in both experimental groups in comparison with the control. Principal component analysis was used to analyse the recorded spectra. It has revealed that higher content of phosphates (wavenumber range 950-1020 $cm^{-1}$) was observed between egg-rich diet fed animals and the control group.
Źródło:
Acta Physica Polonica A; 2012, 121, 2; 555-560
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Investigation of Trace Element Concentration in Diabetic Rats Tissues
Autorzy:
Kowalska, J.
Krośniak, M.
Gryboś, R.
Kwiatek, W.
Powiązania:
https://bibliotekanauki.pl/articles/1809705.pdf
Data publikacji:
2009-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
29.30.Ep
78.70.En
87.19.X-
Opis:
Diabetes is one of the most frequent diseases in developing countries and thus there is a significant interest in diabetes related studies. It was found that vanadium compounds have glucose-lowering properties in diabetes and therefore it is very important to estimate the vanadium dose in diabetes treatment. On the other hand, the proper estimation of vanadium concentration is important due to side effects that occur in vanadium supplementation. In this study the influence of V(IV) and V(V) compounds with different ligands on the concentration of K, Ca, Mn, Fe, Cu, and Zn in selected rat's tissues was investigated by means of proton induced X-ray emission technique. As a result of the measurements it was found that the concentration of vanadium depends on the organ. The highest value was determined in spleen while the lowest in pancreas. It was also found that the concentration of other elements depends on the presence of vanadium and its concentration. The most meaningful influence of vanadium presence was on iron concentration in spleen, on copper and zinc in kidney, and on manganese in pancreas.
Źródło:
Acta Physica Polonica A; 2009, 115, 2; 556-560
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Investigation of Sediments Causing Damage to Water Meters in a Large Drinking Water Distribution System
Autorzy:
Kopeć, M.
Roman, M.
Kąc, M.
Budziak, A.
Paluszkiewicz, C.
Zarzycki, A.
Kąc, S.
Dutkiewicz, E.
Cichoń, T.
Bochnia, T.
Kwiatek, W.
Powiązania:
https://bibliotekanauki.pl/articles/1030652.pdf
Data publikacji:
2018-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
89.60.-k
78.70.En
61.05.cp
82.80.-d
82.80.Gk
Opis:
Preliminary studies on the sediments collected from water meters of Krakow water supply system were performed in the cooperation with the Municipal Water Supply and Sewage. Creation and deposition of sediments on the measuring devices installed in the water supply system is a serious technological and economical problem for water companies, defectively operating for this reason water meters must be replaced. It is evident that knowledge of the chemical and phase composition of sediments is an important step towards resolving the problem of impurities in water supply systems. Four different samples of sediments, coming from water meters, were investigated using the proton-induced X-ray emission, the X-ray diffraction, the Fourier transform infrared and Raman spectroscopy. The X-ray methods revealed presence of amorphous and fine-crystalline phases as well as high content of iron-based compounds. As a crystalline phase, the most frequently appeared: goethite, lepidocrocite, iron oxides (hematite, maghemite, magnetite), calcium carbonate, and quartz. In one of the samples, the nanocrystalline phase was found and identified as hydrous iron oxyhydroxide ferrihydrite. Vibrational spectroscopy methods confirmed the composition of crystalline phases as well as enabled to estimate the abundance of amorphous phase in samples.
Źródło:
Acta Physica Polonica A; 2018, 133, 2; 296-301
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Energy Dissipation in the AFM Elasticity Measurements
Autorzy:
Klymenko, O.
Wiltowska-Zuber, J.
Lekka, M.
Kwiatek, W.
Powiązania:
https://bibliotekanauki.pl/articles/1809585.pdf
Data publikacji:
2009-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
68.37.Ps
87.10.Vg
87.15.La
Opis:
Nowadays, it is well established that changes of cell stiffness observed by atomic force microscopy are linked with the cell cytoskeleton. Its structural and functional alterations are underlying major diseases such as cancer, inflammation or neurodegenerative disorders. So far, the use of atomic force microscopy is mostly focused on the determination of the Young modulus using the modified Hertz model. It can quantitatively describe the elastic properties of living cells, however, its value is burdened by the fact that cells are neither isotropic nor homogeneous material. Often, during the atomic force microscopy measurements, the hysteresis between the loading and unloading curves are observed which indicates the dissipation of an energy. In our studies, the index of plasticity was introduced to enumerate such effect during a single loading-unloading cycle. As the results show, such approach delivers an additional parameter describing the mechanical state of cell cytoskeleton. The analysis was performed on test samples where the mechanical properties of the melanoma cells were changed by glutaraldehyde and cytochalasin D treatments. The non-treated cells were compared with fibroblasts.
Źródło:
Acta Physica Polonica A; 2009, 115, 2; 548-551
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 18

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