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    Wyświetlanie 1-6 z 6
    Tytuł:
    Mechanism of hydroxylamine mutagenesis: An infrared study of the association in non-polar solutions of 5-methyl-Ni^4-hydroxycytosines
    Autorzy:
    Kulińska, Katarzyna
    Psoda, Anna
    Shugar, David
    Powiązania:
    https://bibliotekanauki.pl/articles/1046193.pdf
    Data publikacji:
    1980
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Źródło:
    Acta Biochimica Polonica; 1980, 27, 1; 57-65
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Nucleotide sequence of nuclear tRNAGly genes and tRNAGly pseudogenes from yellow lupin (Lupinus luteus): Expression ofthe tRNAGly genes in vitro and in vivo
    Autorzy:
    Nuc, Przemysław
    Nuc, Katarzyna
    Szweykowska-Kulińska, Zofia
    Pawełkiewicz, Jerzy
    Powiązania:
    https://bibliotekanauki.pl/articles/1044944.pdf
    Data publikacji:
    1997
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Źródło:
    Acta Biochimica Polonica; 1997, 44, 2; 259-274
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Arabidopsis thaliana microRNA162 level is posttranscriptionally regulated via splicing and polyadenylation site selection
    Autorzy:
    Barciszewska-Pacak, Maria
    Knop, Katarzyna
    Jarmołowski, Artur
    Szweykowska-Kulińska, Zofia
    Powiązania:
    https://bibliotekanauki.pl/articles/1038744.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    miRNA
    pri-miRNA
    abiotic stress
    gene expression
    Opis:
    Arabidopsis microRNA162 (miRNA162) level regulation was studied under abiotic stresses, such as drought and salinity. The TaqMan® microRNA assay proved that A. thaliana miRNA162 level was elevated under these stresses, confirming its salt and drought responsiveness. The promoter region analyses of A. thaliana miRNA162a and b genes (MIR162a and MIR162b) identified numerous salinity and drought responsive elements. However, our results indicated that Arabidopsis MIR162a was presumably the main locus responsible for the mature ath-miRNA162 accumulation under the stresses tested, and the MIR162b was generally rather weakly expressed, both in control and under the stress conditions. The MIR162a structure was confirmed to be complex and the pri-miRNA162a hairpin structure was shown to span an alternative exon and an intron. The MIR162a transcription generated a few pri-miRNA162a splicing isoforms that could be functional and non-functional. Upon drought and salinity stresses, the regulation of the pri-miRNA162a alternative splicing pattern revealed an increase of a functional pri-miR162a isoform and a preferential distal polyA site selection under the stress conditions. Apart from the potential transcriptional regulation of the miRNA genes (MIRs) expression, the data obtained point to an essential role of posttranscriptional regulation of Arabidopsis microRNA162 level.
    Źródło:
    Acta Biochimica Polonica; 2016, 63, 4; 811-816
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Developmental changes in barley microRNA expression profiles coupled with miRNA target analysis
    Autorzy:
    Pacak, Andrzej
    Kruszka, Katarzyna
    Swida-Barteczka, Aleksandra
    Nuc, Przemyslaw
    Karlowski, Wojciech
    Jarmolowski, Artur
    Szweykowska-Kulinska, Zofia
    Powiązania:
    https://bibliotekanauki.pl/articles/1038742.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    barley
    microRNA
    deep sequencing
    development
    Opis:
    MicroRNAs are 19- to 24-nt-long single-stranded RNAs that are crucial regulators of gene expression which control plant development and response to environmental cues. We have analyzed microtranscriptomes of five barley developmental stages. Generally, during the barley development, miR168-3p and miR1432-5p levels increase while the 5'U-miR156-5p level decreases (with exception for the 2-week-old barley). We have identified two miR156-5p izomiRs (called 5'U-miR156-5p [20 nt] and 5'UU-miR156-5p [21 nt]), which were expressed differently during barley development. The 5' U-miR156-5p level decreased in 3-week-, 6-week-, and 68-day-old barley, when compared to the 1-week-old plants. Meanwhile, the 5' UU-miR156-5p level increased significantly in the 68-day-old barley plants. Moreover, only the 5' U-miR156 isomiR recognizes and guides unique transcription factor mRNAs from the Squamosa Promoter Binding Protein-Like (SPL) family. We identified many non-canonical microRNAs with changed expression levels during the barley development. Here, we present the profiles of microRNA expression characteristics for particular barley developmental stages. These analyses are accompanied by the experimental degradome analysis of miRNA targets.
    Źródło:
    Acta Biochimica Polonica; 2016, 63, 4; 799-809
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Barley primary microRNA expression pattern is affected by soil water availability
    Autorzy:
    Swida-Barteczka, Aleksandra
    Kruszka, Katarzyna
    Grabowska, Aleksandra
    Pacak, Andrzej
    Jarmolowski, Artur
    Kurowska, Marzena
    Szarejko, Iwona
    Szweykowska-Kulinska, Zofia
    Powiązania:
    https://bibliotekanauki.pl/articles/1038746.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    pri-miRNA
    miRNA
    drought
    rehydration
    barley genotypes
    Opis:
    MicroRNAs are short molecules of 21-24 nt in length. They are present in all eukaryotic organisms and regulate gene expression by guiding posttranscriptional silencing of mRNAs. In plants, they are key players in signal transduction, growth and development, and in response to abiotic and biotic stresses. Barley (Hordeum vulgare) is an economically important monocotyledonous crop plant. Drought is the world's main cause of loss in cereal production. We have constructed a high-throughput Real-Time RT-qPCR platform for parallel determination of 159 barley primary microRNAs' levels. The platform was tested for two drought-and-rehydration-treated barley genotypes (Rolap and Sebastian). We have determined changes in the expression of primary microRNAs responding to mild drought, severe drought, and rehydration. Based on the results obtained, we conclude that alteration in the primary microRNA expression is relative to the stress's intensity. Mild drought and rehydration mostly decrease the pri-miRNA levels in both of the tested genotypes. Severe drought mainly induces the primary microRNA expression. The main difference between the genotypes tested was a much-stronger induction of pri-miRNAs in Rolap encountering severe drought. The primary microRNAs respond dynamically to mild drought, severe drought, and rehydration treatments. We propose that some of the individual pri-miRNAs could be used as drought stress or rehydration markers. The usage of the platform in biotechnology is also postulated.
    Źródło:
    Acta Biochimica Polonica; 2016, 63, 4; 817-824
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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