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    Wyświetlanie 1-4 z 4
    Tytuł:
    Morphological defects of epididymal spermatozoa in male roe deer (Capreolus capreolus) during the reproductive season
    Autorzy:
    Koziol, K.
    Koziorowski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/31576.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The aim of this study was to analyse the morphology of the epididymal spermatozoa of male roe deer obtained postmortem at the beginning (May), peak (July/August) and the end (September) of the reproductive season. Spermatozoal abnormalities were divided into major (associated with impaired fertility) and minor (not associated with impaired fertility) defects. The highest percentage of abnormal spermatozoa was observed in May (17.78 ± 1.88%), with a much higher proportion of major (12.35 ± 1.11%) than minor defects (5.43 ± 1.59%) being observed. The percentage of abnormal spermatozoa was lowest during the peak of the reproductive season (4.97 ± 1.13%), with the proportion of major (2.68 ± 0.78%) and minor defects (2.28 ± 0.45%) being comparable during this period. The percentage of abnormal spermatozoa increased again in September (11.05 ± 1.60%), with the major defects (6.15 ± 1.04%) slightly surpassing the minor defects (4.90 ± 0.77%); however, total abnormalities still remained lower than those found in May. These differences were statistically significant, with the exception of the difference in minor defects between the pre-rut and post-rut periods. These results indicate that the best period to collect epididymal spermatozoa from roe deer postmortem is the peak of the reproductive season (July/August); however, they can also be recovered at the end of the reproductive season (September), as the percentage of major defects is relatively low at this time. This study provides the basis for further research to determine optimal methods for the storage and cryopreservation of epididymal spermatozoa in this species.
    Źródło:
    Polish Journal of Veterinary Sciences; 2015, 18, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of milk from the cows, receiving herbal extracts in their diet on homeostasis of laboratory animals being a model for human
    Wpływ mleka krów otrzymujących ekstrakty z ziół na homeostazę zwierząt laboratoryjnych modelowych dla człowieka
    Autorzy:
    Dymnicka, M.
    Wiesik, M.
    Koziorowski, M.
    Arkuszewska, E.
    Lozicki, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/843248.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Zootechniczne
    Opis:
    The aim of the study was to examine whether extracts from herbal preparation Herbatan and of Echinacea purpurea, being employed as feed additives for dairy cows, and being added directly to milk, affected its functional value. The studies were conducted on Wistar rats, at random classified in 6 groups, 10 animals in each group. The rats from group 1 received milk from the cows which received addition of 150 ml of Herbatan in their diet. The milk for the rats from group 2 derived from the cows fed the diet with the addition of Echinacea purpurea extract (8.6 mg/kg of body weight). In group 3, the animals received 400 μl of Herbatan in their daily ration of milk; in group 4, the rats received the addition of 1.13 ml of Echinacea purpurea extract. The rats in all group received 55 ml of milk per head, in average. The animals of group 5 received milk without herbal extracts and those ones from group 6 were fed the synthetic diet. The following determinations were carried out: body weight, relative body weight of the rats, the selected biochemical parameters in blood serum: TP, ALB, BUN, GLU, TRI, CHOL, HDL, LDL, VLDL, AST and ALT, morphological parameters of red and white cell system, the level of the selected immunological indicators: immunoglobulins IgG, IgE, IgM, Il-2 and Il-4 in blood serum of the rats, phagocytic activity of monocytes and neutrophils of peripheral blood. Histological measurements of intestinal villi were carried out. The effect of herbal extracts on functional value of milk was reflected in: lower content of cholesterol and LDL in the rats fed the milk diet with the direct addition of Herbatan and Echinacea purpurea to the milk as compared to the rats from the remaining groups, in the highest level of erythrocytes and hemoglobin in the rats receiving the milk from the cows fed with the addition of Herbatan, in the intensive fluorescent effect of phagocytic neutrophils in the group of the rats receiving the milk from the cows fed the diet with Herbatan addition and in the group of the rats which received the addition of Echinacea purpurea directly to milk.
    Celem pracy było zbadanie czy ekstrakty z preparatu ziołowego Herbatan i Echinacea purpurea, stosowane jako dodatki paszowe dla krów mlecznych oraz dodawane bezpośrednio do mleka, wpływają na jego wartość funkcjonalną. Badania przeprowadzono na szczurach rasy Wistar, podzielonych losowo na 6 grup po 10 sztuk. Szczury grupy 1. dostawały mleko od krów otrzymujących w dawce pokarmowej dodatek 150 ml Herbatanu. Mleko dla szczurów z grupy 2. pochodziło od krów żywionych z dodatkiem ekstraktu Echinacea purpurea (8,6 mg/kg masy ciała). Do codziennej porcji mleka dla każdego szczura w grupie 3. dodawano 400 ml Herbatanu, a w grupie 4. – 1,13 ml ekstraktu Echinacea purpurea. Szczury we wszystkich grupach pobierały średnio 55 ml mleka na sztukę dziennie. Szczury grupy 5. otrzymywały mleko bez ekstraktów ziołowych, a grupy 6. – dietę syntetyczną. Określano: masę ciała i relatywną masę ciała szczurów, wybrane wskaźniki biochemiczne w surowicy krwi (TP, ALB, BUN, GLU, TRI, CHOL, HDL, LDL, VLDL, AST, ALT), parametry morfologiczne układu czerwono- i białokrwinkowego, poziom wybranych wskaźników odpornościowych: immunoglobulin IgG, IgE, IgM, Il-2 oraz Il-4 w surowicy krwi szczurów, aktywność fagocytarną monocytów i neutrofili krwi obwodowej. Wykonywano pomiary histologiczne kosmków jelitowych. Oddziaływanie ekstraktów ziołowych na wartość funkcjonalną mleka przejawiało się w niższej zawartości cholesterolu i LDL u szczurów w grupach na diecie mlecznej z bezpośrednim dodatkiem do mleka Herbatanu i Echinacea purpurea, natomiast w odniesieniu do szczurów z pozostałych grup – w najwyższej zawartości erytrocytów i hemoglobiny u szczurów otrzymujących mleko od krów żywionych z dodatkiem Herbatanu, w intensywnym oddziaływaniu fluorescencyjnym neutrofili fagocytujących w grupie szczurów otrzymującej mleko od krów z udziałem w dawce Herbatanu oraz w grupie szczurów otrzymującej dodatek Echinacea purpurea bezpośrednio do mleka.
    Źródło:
    Roczniki Naukowe Polskiego Towarzystwa Zootechnicznego; 2012, 08, 2
    1733-7305
    Pojawia się w:
    Roczniki Naukowe Polskiego Towarzystwa Zootechnicznego
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Seasonal-dependent variations in metabolic status of spermatozoa and antioxidant enzyme activity in the reproductive tract fluids of wild boar/domestic pig hybrids
    Autorzy:
    Dziekonska, A.
    Fraser, L.
    Koziorowska-Gilun, M.
    Strzezek, J.
    Koziorowski, M.
    Kordan, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087956.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    This study investigated seasonal changes in the metabolic performance of spermatozoa and activity of the antioxidant enzymes in the seminal plasma of three wild boar/domestic pigs (aged 1.5 to 2.5 years) and the activity of the antioxidant enzymes in fluids of the cauda epididymidis and vesicular glands from 16 wild boar/domestic pig hybrids (aged 1 to 3 years). Parameters of the sperm metabolic activity, such as total motility, mitochondrial functions, and measurements of oxygen uptake, ATP content and L-lactate production, were analyzed during the spring-summer and autumn-winter periods. Besides these sperm metabolic parameters, the sperm membrane integrity was also assessed. Total protein content and activity of the antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), were measured in the reproductive tract fluids. There were no marked significant differences (P > 0.05) between the seasonal periods in terms of sperm motility, mitochondrial function and oxygen uptake; however, spermatozoa collected during the autumn-winter period exhibited higher (P < 0.05) ATP content and L-lactate production than those harvested during the spring-summer period. It was found that the vesicular gland fluid exhibited a higher level of SOD activity during the spring-summer period compared with the autumn-winter period. Furthermore, CAT activity in the seminal plasma and vesicular gland fluid was greater during the autumn-winter. Total protein content was significantly higher in the vesicular gland fluid, whereas the cauda epididymidal fluid exhibited greater SOD and GPx activities, irrespective of the seasonal period. The findings of this study confirmed seasonal-related differences in the metabolic performance of spermatozoa and activity of antioxidant enzymes of the reproductive tract of the boar/domestic pig hybrids.
    Źródło:
    Polish Journal of Veterinary Sciences; 2014, 17, 2; 307-313
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Endogenous carbon monoxide (CO) as a modulating factor of molecular biological clock in the hypothalamic structures involved in light transmission in pig and wild boar hybrid during long and short day season
    Autorzy:
    Gilun, P.
    Koziorowska-Gilun, M.
    Wąsowska, B.
    Sowa-Kućma, M.
    Kozioł, K.
    Romerowicz-Misielak, M.
    Kordan, W.
    Koziorowski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/16647460.pdf
    Data publikacji:
    2023
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    carbon monoxide
    humoral pathway
    light
    master biological clock
    seasonal
    Opis:
    Mature males of a wild boar-pig crossbreed, during the long and short day season, were used for the study which demonstrates that the chemical light carrier CO regulates the expression of biological clock genes in the hypothalamus via humoral pathways. Autologous blood with experimentally elevated concentrations of endogenous CO (using lamps with white light-emitting diodes) was infused into the ophthalmic venous sinus via the right dorsal nasal vein. Molecular biology methods: qPCR and Western Blot were used to determine the expression of genes and biological clock proteins. The results showed that elevated endogenous CO levels, through blood irradiation, induces changes in genes expression involved in the functioning of the main biological clock located in suprachiasmatic nuclei. Changes in the expression of the transcription factors Bmal1, Clock and Npas2 have a similar pattern in both structures, where a very large decrease in gene expression was shown after exposure to elevated endogenous CO levels. The changes in the gene expression of PER 1-2, CRY 1-2, and REV-ERB α-β and ROR β are not the same for both POA and DH hypothalamic structures, indicating that both structures respond differently to the humoral signal received. The results indicate that CO is a chemical light molecule whose production in an organism depends on the amount of light. An adequate amount of light is an essential factor for the proper functioning of the main biological clock.
    Źródło:
    Polish Journal of Veterinary Sciences; 2023, 26, 1; 65-82
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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