- Tytuł:
- Expression of bovine leukemia virus protein p24 in Escherichia coli and its use in the immunoblotting assay.
- Autorzy:
-
Bicka, Leokadia
Kuźmak, Jacek
Kozaczyńska, Bożena
Płucienniczak, Andrzej
Skorupska, Anna - Powiązania:
- https://bibliotekanauki.pl/articles/1044191.pdf
- Data publikacji:
- 2001
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
bovine leukemia virus
fusion protein p24
immunoblotting assay
gag gene cloning - Opis:
- The gag gene encoded protein, p24 of bovine leukemia virus (BLV), was cloned and expressed as thioredoxin-6xHis-p24 protein in Escherichia coli. The bacterial cells carrying plasmid pT7THis-p24 expressed the protein of 38 kDa that was detected by immunoblotting analysis using anti-p24 monoclonal antibodies and sera from BLV infected cattle and sheep. The purified p24 fusion protein was shown to be sensitive and specific for detection of BLV antibodies in the infected cattle.
- Źródło:
-
Acta Biochimica Polonica; 2001, 48, 1; 227-232
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł