- Tytuł:
- Non-enzymatic activation of prothrombin induced by interaction with fibrin β26-42 region
- Autorzy:
-
Chernyshenko, Volodymyr
Chernyshenko, Tamara
Korolova, Dar'ya
Volynets, Galyna
Kolesnikova, Iryna
Platonova, Tetyana
Lugovskoy, Eduard - Powiązania:
- https://bibliotekanauki.pl/articles/1038995.pdf
- Data publikacji:
- 2015
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
prothrombin
fibrin
fibrin degradation products
staphylocoagulase
thrombin - Opis:
- We have discovered that addition of monomeric desAB fibrin to prothrombin leads to appearance of the thrombin-like activity of prothrombin towards S2238 chromogenic substrate. DesA and desABβ(15-42)2 fibrin forms did not cause any activation of prothrombin. From this observation we could suggested that amino acid residues of the 15-42 fragment of BβN-domain presented in desAB fibrin, cleaved in desABβ(15-42)2 fibrin and protected in desA fibrin, play a crucial role in the non-enzymatic activation of prothrombin. To identify the Bβ amino acid residues involved in the fibrin-prothrombin binding we used monoclonal antibodies 1-5G and 2d2a with epitopes in Bβ26-42 and Bβ12-26 fibrin fragments respectively. The thrombin-like activity in the mixture of prothrombin and desAB fibrin was monitored in the presence of each of these monoclonal antibodies. It was found that anti-Bβ12-26 antibody does not exhibit any inhibitory effect on the thombin-like activity of the mixture. In contrast, adding of Bβ26-42 antibody into the mixture of desAB fibrin with prothrombin diminished the thrombin-like activity by 70%. Recombinant dimeric peptides Bβ(15-44)2 and Bβ(15-66)2 that mimic amino acid residues in fibrin were also tested for their ability to activate prothrombin. It was found that both peptides were able to induce non-enzymatic activation of prothrombin. The activation was more evident in the case of Bβ(15-44)2 peptide. From the data obtained we can conclude that desAB fibrin binds to prothrombin through the Bβ26-42 amino acid residues and the formation of such a complex caused a non-enzymatic activation of prothrombin.
- Źródło:
-
Acta Biochimica Polonica; 2015, 62, 3; 517-522
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł