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Wyszukujesz frazę "Kong, Jie" wg kryterium: Autor


Wyświetlanie 1-2 z 2
Tytuł:
Chlamydial genomic MinD protein does not regulate plasmid-dependent genes like Pgp5
Autorzy:
Sun, Yina
Kong, Jie
Ma, Jingyue
Qi, Manli
Zhang, Ying
Han, Long
Liu, Quanzhong
Liu, Yuanjun
Powiązania:
https://bibliotekanauki.pl/articles/1038373.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Chlamydia
MinD
Pgp5
Transformation system
Opis:
Chlamydia has a unique intracellular developmental cycle, which has hindered the single protein function study of Chlamydia. Recently developed transformation system of Chlamydia has greatly advanced the chlamydial protein's function research and was used to find that a chlamydial plasmid-encoded Pgp5 protein can down-regulate plasmid-dependent genes. It is assumed, that chlamydial genomic MinD protein has a similar function to Pgp5. However, it is unknown whether MinD protein regulates the same plasmid-dependent genes. We replaced pgp5 gene in the shuttle vector pGFP::CM with minD gene of C. trachomatis (CT0582) or C. muridarum (TC0871). The recombinant plasmid was transformed into plasmid-free organisms-CMUT3 and qRT-PCR was used to detect the transcription level of plasmid-encoded and -dependent genes in these pgp5 deficient organisms. As a readout, GlgA, one of the plasmid-regulated gene products was detected by immunofluorescence assay. After recombination, transformation and plaque purification, the stable transformants CT0582R and TC0871R were generated. In these transformants, the plasmid-dependent genes were up-regulated, alike in the pgp5 premature stop mutant and pgp5 replacement with mCherry mutant. GlgA protein level was also increased in all pgp5 mutants, including CT0582R and TC0871R. Thus, our study showed that genomic MinD protein had different function than Pgp5, which was useful for further understanding the chlamydiae.
Źródło:
Acta Biochimica Polonica; 2018, 65, 3; 425-429
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Serum activities of liver enzymes in workers exposed to sub-TLV levels of dimethylformamide
Autorzy:
He, Jinjiang
Liu, Jie
Kong, Yulin
Yang, Weiwei
Zhang, Zengli
Powiązania:
https://bibliotekanauki.pl/articles/2177403.pdf
Data publikacji:
2015-03-27
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
dimethylformamide
workers
liver enzymes
TWA
sub-TLV
urine
Opis:
Objectives The aim of this study has been to investigate serum activities of liver enzymes in workers exposed to sub-TLV levels of dimethylformamide (DMF). Material and Methods Seventy-two workers and 72 healthy controls participated in the study. All subjects underwent complete physical examinations and abdominal ultrasound examination. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and c-glutamyl transpeptidase (c-GT) were determined by an auto-chemistry analyzer. The data of airborne concentrations of DMF was obtained from the local Center of Disease Control and Prevention. The level of urine N-acetyl-S-(N-methylcarbamoyl)cysteine (AMCC) was measured by means of high-performance liquid chromatography. Results Time weighted average (TWA) concentration of the DMF in workplace was 18.6 (range: 9.8–36.2) mg/m³. The concentration of the AMCC in workers’ urine was 28.32 (range: 1.8–58.6) mg/l and 9 workers’ AMCC exceeded the biological exposure index (40 mg/l). Thirty-one workers reported gastrointestinal symptoms (abdominal pain, nausea, anorexia) and 10 workers reported headache, dizziness and/or palpitation in the exposed group. Serum analysis revealed that both the mean of serum activities of liver enzymes (ALT, AST and c-GT) and the percentage of workers with abnormal liver function were significantly higher in the exposed group as compared to the controls. Conclusions Dimethylformamide can cause liver damage even if air concentration is in the sub-threshold limit value (sub-TLV) level. The protection of skin contact against the exposure to the DMF might be a critical issue as far as the occupational health is concerned.
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2015, 28, 2; 395-398
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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