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    Wyświetlanie 1-6 z 6
    Tytuł:
    Application of nine species-specific microsatellite loci to characterize three pike-perch (Sander lucioperca) populations from the Aral Sea basin in Uzbekistan
    Autorzy:
    Khurshut, E.
    Kohlmann, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/363116.pdf
    Data publikacji:
    2009
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    sandacz
    markery mikrosatelitarne
    heterozygotyczność
    zróżnicowanie genetyczne
    pike-perch
    microsatellite markers
    heterozygosities
    genetic differentiation
    Opis:
    The pike-perch is one of the main commercial fish species in Uzbekistan. Historically, it inhabited the Aral Sea and deltas of inflowing rivers (Amu-Darya and Syr-Darya). At the beginning of the 1960s it was introduced into water bodies of the middle Amu-Darya and Syr-Darya from the Ural River. The present study was aimed to evaluate the current genetic status of wild pike-perch populations from different water bodies using nine recently isolated species-specific microsatellite markers. The three examined Uzbek pike-perch populations expressed significantly higher average numbers of alleles per locus (5.56 to 14.22) than three year-classes of a German wild population (3.11 to 3.78) included for comparison. On the other hand, average observed and expected heterozygosities were not significantly different in both regions (0.743 and 0.760 in Uzbek pike-perch; 0.576 and 0.471 in German pike-perch). After sequential Bonferroni corrections all Uzbek populations were found to be in Hardy-Weinberg equilibrium. Genetic differentiation between all populations as estimated by FST values was statistically significant. Pike-perch from Amu-Darya River delta and Tudakul reservoir were less differentiated from each other probably due to gene flow between them (both water bodies are connected) whereas the more distinct Syr-Darya River population showed indication for introgression with non-native individuals stocked in that region. Since stocking is widely practiced to satisfy the growing demand of pike-perch fisheries in Uzbekistan, fish seed for such activities should be obtained from local, genetically undisturbed populations such as the Amu-Darya River delta population.
    Źródło:
    Environmental Biotechnology; 2009, 5, 1; 3-10
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A simple, rapid method for the production of soluble recombinant proteins from the duplicated growth hormone genes of the tetraploid common carp
    Autorzy:
    Murakaeva, A.
    Kohlmann, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/363072.pdf
    Data publikacji:
    2011
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    karp
    powielanie genu
    powielanie genomu
    hormon wzrostu
    common carp
    gene duplication
    genome duplication
    growth hormone
    Opis:
    The simple protein production method developed in the present study enables the production of large quantities of active recombinant common-carp growth hormone proteins in a few brief steps. With this method, the recombinant protein is obtained in a soluble form without the need for refolding. This method can be utilised for determining if the two common-carp growth hormones have similar functions in vitro and in vivo and what role each plays in growth regulation. If differences in activity exist, this method can also be used to produce intermediate forms of the two common carp growth hormones rapidly in order to determine which of the six amino acids that differ between the two proteins are responsible for the altered activity. Common carp has the same tetraploid ancestor as goldfish, and the products of the growth hormone paralogues in goldfish also differ. It would be interesting to understand how the same duplicated orthologues have evolved, and the methods elaborated in this study could be used for such comparative investigations.
    Źródło:
    Environmental Biotechnology; 2011, 7, 2; 53-64
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Tracing the genetic origin of brown trout (Salmo trutta) re-colonizing the Ecker reservoir in the Hartz National Park, Germany
    Autorzy:
    Kohlmann, K.
    Wüstemann, O.
    Powiązania:
    https://bibliotekanauki.pl/articles/363212.pdf
    Data publikacji:
    2012
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    pstrąg potokowy
    Salmo trutta
    mikrosatelitarne DNA
    DNA
    PCR
    brown trout
    microsatellite
    Opis:
    The Ecker reservoir and its main tributary had been free of brown trout (Salmo trutta) for several decades due to cumulative effects of natural and anthropogenic acidification. However, after the decline of emissions in the 1990s and the resulting rise of water pH to suitable for brown trout values, the species began to recolonize its original habitats. In the main tributary first brown trout individuals were caught in 2008 and in the reservoir in later years as well. Stocking could be excluded in both areas. Therefore, the present study was aimed to trace the genetic origin of these brown trout by genotyping eight microsatellite loci in samples collected in the reservoir, its main tributary, potential refugia and - for comparison - from two areas downstream of the dam being physically isolated for about 70 years. Genetic variability within populations (average number of alleles per locus), genetic differentiation between populations (FST values and genetic distances), occurrence of certain alleles and results of assignment tests indicated that the Ecker reservoir was re-colonized from two sources: the Große Peseke, a small direct inflow into the reservoir, and the Fuhler Lohnsbach, a parallel flowing brook connected to the reservoir by a pipe. Genetic data also supported recolonization of the main tributary from the reservoir but not in the opposite direction. Moreover, bottleneck effects were evident in brown trout populations upstream of the dam compared to the two populations downstream of the dam.
    Źródło:
    Environmental Biotechnology; 2012, 8, 2; 39-44
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Polymorphism of microsatellite loci - a tool in studying biodiversity of paddlefish aquaculture broodstock
    Autorzy:
    Kaczmarczyk, D.
    Kohlmann, K.
    Kersten, P.
    Luczynski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/363088.pdf
    Data publikacji:
    2007
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    izolacja DNA
    DNA mikrosatelitarne
    amplifikacja PCR
    DNA extraction
    microsatellite DNA
    PCR amplification
    Opis:
    American paddlefish (Polyodon spathula) is a new species in Polish aquaculture, its broodstocks are few and small, and it is possible that all mature fish originated from only a few spawners. Studies on polymorphism of highly variable microsatellite DNA allow revealing genetic characteristics of individual spawners as well as estimation of genetic variation within and divergence between broodstocks. This paper describes optimised protocols for isolation of DNA from fin tissues, amplification of nine microsatellite loci using PCR technique, and for fish genotyping using automatic capillary DNA sequencer. Our technique was tested towards the fin samples taken from all paddlefish reared in Poland and approaching their sexual maturity; the study included also samples taken from 47 fish of the Ukrainian breeding center (Gorny Tykich).
    Źródło:
    Environmental Biotechnology; 2007, 3, 2; 44-48
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    New microsatellite multiplex PCR sets for genetic studies of the sterlet sturgeon, Acipenser ruthenus
    Autorzy:
    Kohlmann, K.
    Kersten, P.
    Geßner, J.
    Onără, D.
    Taflan, E.
    Suciu, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/363202.pdf
    Data publikacji:
    2017
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    Acipenser ruthenus
    microsatellite
    multiplex PCR
    species conservation
    sustainable fisheries
    Opis:
    Wild populations of the sterlet sturgeon, Acipenser ruthenus, are declining throughout their native ranges. In-depth knowledge of their genetic diversity and structure is urgently needed to enable the identification of management units for conservation purposes. Moreover, genetic markers are required to establish appropriate breeding schemes for supportive stocking programs and to monitor genetic changes in farmed stocks. Therefore, six species-specific, polymorphic microsatellite loci were isolated and arranged into five multiplex PCR sets together with nine loci from other sturgeon species. The diversity of these 15 microsatellites was examined in 67 sterlet individuals (20 farmed in Germany and 47 wild-caught in the Romanian part of the River Danube). The total number of alleles per locus ranged from 3 to 15 with an average of 7.20. The farmed sterlet sturgeon possessed 1 to 7 alleles per locus, with a mean of 3.13; the wild individuals were more variable, with 3 to 15 alleles per locus and a mean of 7.07. Observed heterozygosities ranged from 0 to 0.850 in the farmed individuals, and from 0.064 to 0.957 in the wild individuals. Indications of inbreeding were only found in the wild sterlet sturgeon (FIS=0.062). The genetic differentiation of the two sterlet groups was significant (FST=0.1186). The high sensitivity and discriminatory power of the 15 loci was indicated by the very low overall probability of identity for siblings (PIsib=5.099x10-5) and the high accuracy of self-classification (66 out of the 67 individuals (98.51%) were correctly identified). Thus, these newly developed multiplex PCR sets are a valuable genetic tool for identifying management units for species conservation, sustainable fisheries and aquaculture.
    Źródło:
    Environmental Biotechnology; 2017, 13, 1; 11-17
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detection of two major cytochrome b lineages in pike-perch, Sander lucioperca, and first data on their distribution in European populations
    Autorzy:
    Kohlmann, K.
    Louati, M.
    Kersten, P.
    Bahri-Sfar, L.
    Poulet, N.
    Ben Hassine, O. K.
    Powiązania:
    https://bibliotekanauki.pl/articles/363206.pdf
    Data publikacji:
    2013
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    cytochrome b
    PCR-RFLP
    pike perch
    Sander lucioperca
    cytochrom b
    sandacz
    Opis:
    Despite of the growing interest in pike-perch for aquaculture and its economic importance in fisheries, knowledge on the population structure and phylogeography of the species is still limited. We report the discovery of two major cytochrome b lineages and describe a simple method for their detection based on PCR amplification followed by restriction digestion with Alw26I. Screening of 708 individuals showed that haplotype A was fixed or dominating in Central and East European countries, whereas haplotype B was mainly found in several French populations and Tunisian pike-perch introduced from Europe. Sequencing the complete cytochrome b cds of 17 representative individuals revealed that haplotypes A and B differed by five substitutions but also showed further differentiation of both haplotypes due to an additional substitution in a single haplotype A and B individual from France, respectively. Five partial pike-perch cytochrome b cds available from NCBI GenBank could also clearly be assigned to one or the other of the two major lineages. Therefore, this new mtDNA marker might be considered as suitable not only for studies on population structure and phylogeography of pikeperch but also to trace its introduction history and to assess the genetic composition of aquaculture brood stocks.
    Źródło:
    Environmental Biotechnology; 2013, 9, 1; 1-5
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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