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    Wyświetlanie 1-6 z 6
    Tytuł:
    Progesterone biosynthesis supported by fatty acid oxidation in the mitochondrial fraction of human term placenta
    Autorzy:
    Tiałowska, Barbara
    Klimek, Jerzy
    Żelewski, Leon
    Powiązania:
    https://bibliotekanauki.pl/articles/1046080.pdf
    Data publikacji:
    1983
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Źródło:
    Acta Biochimica Polonica; 1983, 30, 1; 11-21
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Inhibition by fatty acids of the cholesterol side-chain cleavage in the course of progesterone biosynthesis in the mitochondrial fraction of human term placenta
    Autorzy:
    Tiałowska, Barbara
    Klimek, Jerzy
    Żelewski, Leon
    Powiązania:
    https://bibliotekanauki.pl/articles/1046164.pdf
    Data publikacji:
    1980
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Źródło:
    Acta Biochimica Polonica; 1980, 27, 3-4; 257-264
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    AMP-deaminase from hen stomach smooth muscle - physico-chemical properties of the enzyme.
    Autorzy:
    Swieca, Anna
    Rybakowska, Iwona
    Koryziak, Anna
    Klimek, Jerzy
    Kaletha, Krystian
    Powiązania:
    https://bibliotekanauki.pl/articles/1043348.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    AMP-deaminase
    smooth muscle
    Opis:
    AMP-deaminase from hen stomach smooth muscle was isolated and physico-chemical properties of the purified enzyme were investigated. The enzyme had an activity optimum at pH 6.5, and poorly deaminated the substrate analogues tested. At optimum pH (6.5), in the absence of regulatory ligands (control conditions), the enzyme manifested hyperbolic substrate-saturation kinetics with half-saturation constant (S0.5) of about 4.5 mM. Additions of adenine nucleotide effectors (ATP, ADP) activated the enzyme strongly at all the concentrations tested, diminishing significantly the value of S0.5 constant. In contrast, the regulatory effect of orthophosphate was variable, and depended on the orthophosphate concentration used. The molecular mass of the enzyme subunit determined in SDS/PAG electrophoresis was about of 37 kDa. The obtained results suggest that in different types of hen muscle, similarly as in humans and rats, expression of AMP-deaminase is under the control of independent genes.
    Źródło:
    Acta Biochimica Polonica; 2004, 51, 1; 213-218
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Partial characterization of human choriocarcinoma cell line JAR cells in regard to oxidative stress.
    Autorzy:
    Hallmann, Anna
    Klimek, Jerzy
    Masaoka, Makoto
    Kamiński, Marcin
    Kędzior, Jakub
    Majczak, Anna
    Niemczyk, Edyta
    Woźniak, Michał
    Trzonkowski, Piotr
    Wakabayashi, Takashi
    Powiązania:
    https://bibliotekanauki.pl/articles/1041517.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    menadione
    flow cytometry
    JAR cells
    placenta
    H2O2
    oxidative stress
    Opis:
    Characterization of free radical-induced cell injury processes of placenta cells is of vital importance for clinical medicine for the maintenance of intrauterine fetal life. The present study has analyzed cell injury processes in cells of the choriocarcinoma cell line JAR treated with menadione, an anticancer drug, and Hg2O2 in comparison to osteosarcoma 143B cells using electron microscopic and flow cytometric techniques. Flow cytometry on JAR cells exposed to 100 μM menadione and double-stained with Annexin V and propidium iodide (PI) detected apoptotic cells reaching the maximum after 4 h of incubation with a rapid decrease thereafter. Viable cells became decreased to 46% of the control after 2 h of incubation, reaching 5% after 4 h. Cells stainable with both Annexin V and PI began to increase distinctly after 2 h of incubation, reaching 55% after 4 h. Electron microscopy showed that cells stainable with both dyes specified above had condensed nuclei and swollen cytoplasm, suggesting that they were undergoing a switch of the cell death mode from apoptosis to necrosis. On the other hand, 90% of 143B cells remained intact after 4 h of menadione treatment although the intracellular levels of superoxide were always higher than those of JAR cells treated with the drug. In contrast, JAR cells were more resistant than 143B cells to H2O2-induced cytotoxicity. These results may suggest that cytotoxicity of menadione cannot be explained simply by oxygen free radicals generated from the drug. The resistance of JAR cells to oxygen free radical-induced cytotoxicity may be advantageous for intrauterine fetal life.
    Źródło:
    Acta Biochimica Polonica; 2004, 51, 4; 1023-1038
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Bentonite from the Central Slovakia Volcanic Field – A prospective raw material for Polish industry
    Autorzy:
    Górniak, Katarzyna
    Szydłak, Tadeusz
    Gaweł, Adam
    Klimek, Agnieszka
    Tomczyk, Anna
    Motyka, Jerzy
    Bahranowski, Krzysztof
    Powiązania:
    https://bibliotekanauki.pl/articles/127637.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Mineralogiczne
    Tematy:
    commercial bentonite
    Central Slovakia Volcanic Field
    petrography
    smectite
    microscopic imaging
    Opis:
    This paper summarizes information about recently worked bentonite deposits in Slovakia and presents the results of studies on bentonite from the Central Slovakia Volcanic Field (CSVF). The authors compared the mineralogy of commercial bentonites exploited in the Stara Kremnička (Jelšový potok), Kopernica, and Hliník nad Hronom deposits. X-ray diffraction (XRD), chemical analyses and microscopy showed that the main component is montmorillonite (37–88%), followed by opal C/CT (5–25%), clinoptilolite (up to 15%), feldspars (3–12%), quartz (up to 8%), biotite (2–5%), and kaolinite (up to 2%). The microscopic imaging provided information valuable for the technological assessment of bentonites, particularly the evaluation of mineralogy determined by XRD. The low variability of the mineral composition of commercial bentonites exploited in the western CSVF, together with the significant reserves and localization of deposits close to the Polish–Slovak state border prove that this raw material deserves more attention from Polish industry.
    Źródło:
    Mineralogia; 2017, 48, 1/4; 23-38
    1899-8291
    1899-8526
    Pojawia się w:
    Mineralogia
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Mitochondria-associated satellite I RNA binds to hnRNP K protein
    Autorzy:
    Klimek-Tomczak, Karolina
    Mikula, Michał
    Dzwonek, Artur
    Paziewska, Agnieszka
    Wyrwicz, Lucjan
    Hennig, Ewa
    Ostrowski, Jerzy
    Powiązania:
    https://bibliotekanauki.pl/articles/1041284.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    hnRNP K protein
    SAGE
    mitochondria
    satellite I RNA
    Opis:
    hnRNP K protein, which localizes to the nucleus, cytoplasm and mitochondria, is involved in the various cellular processes that compose gene expression. We used a SAGE-based assay to profile RNAs associated with hnRNP K protein in rat mitochondria. RNA was isolated from mitoplasts obtained from highly purified and RNase-treated mitochondria. Total RNA and RNA associated with hnRNP K protein were then used as input material for generating two SAGE libraries. Mitochondrion-derived tags isolated from the total mitoplast RNA library represented 86.3%, while those isolated from the library constructed from RNA associated with hnRNP K protein represented only 28.2% of selected tags. Thus, an unexpected number of nuclear-encoded RNAs were purified from mitochondria. Many of these transcripts were co-purified with hnRNP K protein, and high levels of nuclear-encoded RNAs co-immunoprecipitating with K protein corresponded to elevated hnRNP K protein levels of the organelle. The most abundant RNAs that were co-purified with hnRNP K protein represented transcripts originating from satellite I DNA. While satellite I RNA levels were higher in the nucleus and cytoplasm than in mitochondria, the most abundant binding of satellite I transcripts to hnRNP K protein was found in mitochondria. The role of satellite I RNA in mitochondria remains to be elucidated.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 1; 169-178
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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