- Tytuł:
- Fluorescence Decay Heterogeneity Model Based on Electron Transfer Processes in an Enzyme-Ligand Complex
- Autorzy:
-
Wlodarczyk, J.
Kierdaszuk, B. - Powiązania:
- https://bibliotekanauki.pl/articles/2043367.pdf
- Data publikacji:
- 2005-06
- Wydawca:
- Polska Akademia Nauk. Instytut Fizyki PAN
- Tematy:
- 33.50.-j
- Opis:
- The models are described for complex fluorescence decay of tyrosine in proteins involving continuous distribution of fluorescence lifetimes and electron transfer processes. We introduce the analytical decay function with a power-like term, which provides good fits to highly complex fluorescence decays. Moreover, the power-like term in the proposed decay functions is a manifestation of so-called Tsallis nonextensive statistics and is suitable for description of the systems with long-range interactions, memory effect, as well as with fluctuations of the characteristic lifetime of fluorescence. The proposed decay functions were applied to analysis of fluorescence decays of tyrosine in a protein, i.e. the enzyme purine nucleoside phosphorylase from E. coli, free in aqueous solution and in the complex with formycin A (an inhibitor) and orthophosphate (a co-substrate), and demonstrated that both models reflect the enzyme-ligand interactions. Direct measure of heterogeneity of the enzyme systems is provided by a variance of fluorescence lifetime distribution. The possible number of deactivation channels and excited state mean lifetime can be easily derived without a priori knowledge of the complexity of studied system.
- Źródło:
-
Acta Physica Polonica A; 2005, 107, 6; 883-894
0587-4246
1898-794X - Pojawia się w:
- Acta Physica Polonica A
- Dostawca treści:
- Biblioteka Nauki
Artykuł