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Wyszukujesz frazę "Kersten, P." wg kryterium: Autor

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    Wyświetlanie 1-3 z 3
    Tytuł:
    Polymorphism of microsatellite loci - a tool in studying biodiversity of paddlefish aquaculture broodstock
    Autorzy:
    Kaczmarczyk, D.
    Kohlmann, K.
    Kersten, P.
    Luczynski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/363088.pdf
    Data publikacji:
    2007
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    izolacja DNA
    DNA mikrosatelitarne
    amplifikacja PCR
    DNA extraction
    microsatellite DNA
    PCR amplification
    Opis:
    American paddlefish (Polyodon spathula) is a new species in Polish aquaculture, its broodstocks are few and small, and it is possible that all mature fish originated from only a few spawners. Studies on polymorphism of highly variable microsatellite DNA allow revealing genetic characteristics of individual spawners as well as estimation of genetic variation within and divergence between broodstocks. This paper describes optimised protocols for isolation of DNA from fin tissues, amplification of nine microsatellite loci using PCR technique, and for fish genotyping using automatic capillary DNA sequencer. Our technique was tested towards the fin samples taken from all paddlefish reared in Poland and approaching their sexual maturity; the study included also samples taken from 47 fish of the Ukrainian breeding center (Gorny Tykich).
    Źródło:
    Environmental Biotechnology; 2007, 3, 2; 44-48
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    New microsatellite multiplex PCR sets for genetic studies of the sterlet sturgeon, Acipenser ruthenus
    Autorzy:
    Kohlmann, K.
    Kersten, P.
    Geßner, J.
    Onără, D.
    Taflan, E.
    Suciu, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/363202.pdf
    Data publikacji:
    2017
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    Acipenser ruthenus
    microsatellite
    multiplex PCR
    species conservation
    sustainable fisheries
    Opis:
    Wild populations of the sterlet sturgeon, Acipenser ruthenus, are declining throughout their native ranges. In-depth knowledge of their genetic diversity and structure is urgently needed to enable the identification of management units for conservation purposes. Moreover, genetic markers are required to establish appropriate breeding schemes for supportive stocking programs and to monitor genetic changes in farmed stocks. Therefore, six species-specific, polymorphic microsatellite loci were isolated and arranged into five multiplex PCR sets together with nine loci from other sturgeon species. The diversity of these 15 microsatellites was examined in 67 sterlet individuals (20 farmed in Germany and 47 wild-caught in the Romanian part of the River Danube). The total number of alleles per locus ranged from 3 to 15 with an average of 7.20. The farmed sterlet sturgeon possessed 1 to 7 alleles per locus, with a mean of 3.13; the wild individuals were more variable, with 3 to 15 alleles per locus and a mean of 7.07. Observed heterozygosities ranged from 0 to 0.850 in the farmed individuals, and from 0.064 to 0.957 in the wild individuals. Indications of inbreeding were only found in the wild sterlet sturgeon (FIS=0.062). The genetic differentiation of the two sterlet groups was significant (FST=0.1186). The high sensitivity and discriminatory power of the 15 loci was indicated by the very low overall probability of identity for siblings (PIsib=5.099x10-5) and the high accuracy of self-classification (66 out of the 67 individuals (98.51%) were correctly identified). Thus, these newly developed multiplex PCR sets are a valuable genetic tool for identifying management units for species conservation, sustainable fisheries and aquaculture.
    Źródło:
    Environmental Biotechnology; 2017, 13, 1; 11-17
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detection of two major cytochrome b lineages in pike-perch, Sander lucioperca, and first data on their distribution in European populations
    Autorzy:
    Kohlmann, K.
    Louati, M.
    Kersten, P.
    Bahri-Sfar, L.
    Poulet, N.
    Ben Hassine, O. K.
    Powiązania:
    https://bibliotekanauki.pl/articles/363206.pdf
    Data publikacji:
    2013
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    cytochrome b
    PCR-RFLP
    pike perch
    Sander lucioperca
    cytochrom b
    sandacz
    Opis:
    Despite of the growing interest in pike-perch for aquaculture and its economic importance in fisheries, knowledge on the population structure and phylogeography of the species is still limited. We report the discovery of two major cytochrome b lineages and describe a simple method for their detection based on PCR amplification followed by restriction digestion with Alw26I. Screening of 708 individuals showed that haplotype A was fixed or dominating in Central and East European countries, whereas haplotype B was mainly found in several French populations and Tunisian pike-perch introduced from Europe. Sequencing the complete cytochrome b cds of 17 representative individuals revealed that haplotypes A and B differed by five substitutions but also showed further differentiation of both haplotypes due to an additional substitution in a single haplotype A and B individual from France, respectively. Five partial pike-perch cytochrome b cds available from NCBI GenBank could also clearly be assigned to one or the other of the two major lineages. Therefore, this new mtDNA marker might be considered as suitable not only for studies on population structure and phylogeography of pikeperch but also to trace its introduction history and to assess the genetic composition of aquaculture brood stocks.
    Źródło:
    Environmental Biotechnology; 2013, 9, 1; 1-5
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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