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    Wyświetlanie 1-6 z 6
    Tytuł:
    Mechanisms of interaction of selected environmental factors on the formation of pathophysiological changes in cataracts
    Autorzy:
    Kempka, Katarzyna
    Kamiński, Piotr
    Lorek, Małgorzata
    Malukiewicz, Grażyna
    Bogdzińska, Maria
    Powiązania:
    https://bibliotekanauki.pl/articles/1030797.pdf
    Data publikacji:
    2020
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    antioxidant
    biotechnology
    cataract
    oxidative stress
    reactive oxygen species
    Opis:
    Biotechnology is a relatively young interdisciplinary discipline of science. Thanks to her techniques, we can currently do a lot of research. Biotechnologists in cooperation with other scientists are trying to develop new standards that would become the future of treatment for many diseases, including cataracts. Today, cataracts are increasingly recognized as a civilization disease of the eyes. Unfortunately, if left untreated, it can lead to complete blindness. The etiology of this disease is complex and not fully understood. At present, its foundations are being sought to be able to diagnose her faster and to introduce appropriate treatment early. Literature data currently indicate that it can arise as a result of: age- related changes, genetic (congenital) conditions, inflammation, metabolic disorders, the effects of certain drugs, and complications of eye diseases. From the latest reports we learn that antioxidation can have positive effects when dissolving the protein directly affecting the formation of cataracts. Biotechnology has amazing opportunities to conduct research that can help bring closer the etiology of cataracts and start to treat the disease faster by which so many people in the world lose sight.
    Źródło:
    World Scientific News; 2020, 145; 115-127
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Application of biotechnological methods in stem cell transplantation
    Autorzy:
    Kempka, Katarzyna
    Kamiński, Piotr
    Kasprzak, Mariusz
    Malukiewicz, Grażyna
    Bogdzińska, Maria
    Powiązania:
    https://bibliotekanauki.pl/articles/1065281.pdf
    Data publikacji:
    2019
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    bioreactor
    biotechnological methods
    stem cells
    transplantology
    Opis:
    Currently much attention is paid to the use of stem cells in transplantation. The reason for this interest are the characteristic regenerative capabilities of stem cells and their ability to regenerate and differentiate. Stem cells are becoming the basic unit in regenerative medicine and are increasingly used in transplantation. Currently used innovative methods during cell culture are also a field of activity for biotechnology, which deals with the improvement of methods and looks for new applications of stem cells. The work aims to familiarize the properties and applications of stem cells and present selected techniques for their culture for transplantation. Based on the collected data, it can be concluded that new sources of stem cells should be constantly sought. The improve and create new diagnostic tools and culture media, as well as construct systems that would allow stem cells to be cultured with right orientation.
    Źródło:
    World Scientific News; 2019, 135; 188-201
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Initial pro-antioxidant reactions in the patients suffering from cataract in the interactions with cadmium and lead
    Autorzy:
    Kempka, Katarzyna
    Kamiński, Piotr
    Malukiewicz, Grażyna
    Bogdzińska, Maria
    Florczak, Sylwia
    Powiązania:
    https://bibliotekanauki.pl/articles/1164124.pdf
    Data publikacji:
    2018
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    cadmium
    cataract
    environmental pollution
    lead
    pro-antioxidant
    Opis:
    Cataract is one of the main causes of vision loss. So far, it has been found that one of the causes of cataract formation is the accumulation of heavy metals in ocular tissues and environmental pollution. Research material consisted of blood from healthy volunteers (n = 81; Mogilno Lakeland) and those suffering from cataract (n = 90) from Ophthalmology Clinic, Bydgoszcz (2013, 2016). Samples were prepared in order to obtain plasma, which was used to analyze Fe, Cu, Zn, Mn, Pb, Cd, and Hg concentration (ICP-MS). The purpose of this study is to show the interaction of cataract with cadmium and lead during initial pro-antioxidative reactions and to study element-element interactions. To this end, we analyzed physiological mechanisms that aim to stop the destructive effects of toxic metals. We have shown that the action of zinc, copper and manganese is antioxidant. Concentration of these elements is higher (α = 0. 05, p < 0.05) in the cataract group than in the control. We found interactions Fe-Cu-Zn-Mn-Pb-Cd-Hg. Cd and Pb show a negative effect on the eye tissue and generate pathophysiological changes leading to lens opacity and influence the destabilization of pro-antioxidant reactions. Correlations of elements in the control (Cd-Zn: R = 0.240; Cd-Cu: R = 0.316) and in the sick group (Cd-Cu; R = 0.329) were significant. On the basis of our research we found that there are numerous relationships between the concentrations of chemical elements in the patients with cataracts and the controls. Significant role of antagonisms with cadmium and lead has been demonstrated; these elements contribute to the gradual development of cataract. A correlation between elements exhibiting pro-antioxidant activity in the interactions with cadmium and lead (p=0.000) can be a source of cataract changes. The differences in Cd and Pb concentration and their interactions with elements between the control and sick groups can be considered as a factor for the development of cataract.
    Źródło:
    World Scientific News; 2018, 108; 195-206
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    AGEs interactions with RAGE and their contribution to diabetic retinopathy
    Autorzy:
    Solan, Anna
    Kempka, Katarzyna
    Kamiński, Piotr
    Bogdzińska, Maria
    Malukiewicz, Grażyna
    Powiązania:
    https://bibliotekanauki.pl/articles/1075668.pdf
    Data publikacji:
    2019
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    RAGE
    diabetic retinopathy
    molecular basis
    polimorphism
    signaling pathway
    Opis:
    Diabetic retinopathy as a complication of diabetes is one of the most common causes of vision loss. It is a dangerous disease, which affects more and more people. It is important to know its different causes, background and to generate new, effective therapeutic threatments. One of the possible causes of diabetic retinopathy is the accumulation of advanced glycation products and over-expression of AGEs receptors (RAGE) leading to tissue damage, oxidative stress formation, and the promotion of other changes and disorders. There are many genetic variants that encode the AGEs receptor (RAGE). Some polymorphisms (Gly82Ser, 1704G/T, 2184A/G, -429T/C, -374T/A) may be associated with diabetic retinopathy. This paper aims to systematize knowledge in this field and to show the relationship between these compounds and diabetic retinopathy.
    Źródło:
    World Scientific News; 2019, 119; 41-51
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    DNA isolation method from human blood with MasterPure DNA Purification Kit™ – review article
    Autorzy:
    Florczak, Sylwia
    Kempka, Katarzyna
    Hołderna, Karolina
    Stanek, Emilia
    Baszyński, Jędrzej
    Kamiński, Piotr
    Bogdzińska, Maria
    Powiązania:
    https://bibliotekanauki.pl/articles/1179641.pdf
    Data publikacji:
    2017
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    DNA
    human
    isolation
    optimization
    review
    whole blood
    Opis:
    The main element of genomic molecular analysis is isolation of DNA from biological material. DNA isolation is an important process of PCR methods. Efficency of PCR processes depends of purness and concentration of DNA. Many methods allows to izolate good quality DNA. There are many Purification Kits and methods of extraction genomic material. We show available techniques and methods of increasing efficiency of the process in our studies. Previous data shows that operating with kits needs optimization, whilst protocols do not have all information about isolation process. We concentrate on Epicentre MasterPure DNA Purification Kit, which is cheap, fast and allows to purification nucleic acids from various materials. We show detailed advantages and disadvantages of this set. Our main aim is to develop protocol to get optimal concentration (50-100 ng/μL) and pureness (A 260/280; 1.6-1.9) of DNA. We also should optimize the process of DNA isolation to obtain efficiency of about 100%. Optimized methodology of DNA isolation, with using MasterPure DNA Purification Kit allows to get good quality material in laboratory without specialistic equipment. This survey contains our assumptions of ways how we can influent on purification process according to previous data.
    Źródło:
    World Scientific News; 2017, 72; 69-76
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Badania dostępności architektoniczno-urbanistycznej w Polsce. Studium przypadku
    Research on architectural and urban accessibility in Poland. Case studies
    Autorzy:
    Ujma-Wąsowicz, Katarzyna
    Benek, Iwona
    Kempka, Ada
    Powiązania:
    https://bibliotekanauki.pl/articles/1848925.pdf
    Data publikacji:
    2021
    Wydawca:
    PWB MEDIA Zdziebłowski
    Tematy:
    projektowanie uniwersalne
    audyt architektoniczny
    dostępność architektoniczna
    świadomość
    potrzeba
    ograniczenie
    osoba niepełnosprawna
    universal design
    architectural audit
    architectural accessibility
    awareness
    need
    limitation
    disabled person
    Opis:
    W związku z wprowadzaniem i wdrażaniem w Polsce programu „Dostępność Plus” obserwuje się duże zainteresowanie projektowaniem uniwersalnym przy równocześnie niskim poziomie świadomości potrzeb, a także ograniczeń osób z niepełnosprawnościami. Artykuł przedstawia wybrane badania oraz płynące z nich wnioski w obszarze wdrażania dostępności architektoniczno-urbanistycznej dla osób z niepełnosprawnościami w miastach Regionu Górnego Śląska. Celem badań jest przede wszystkim gromadzenie niezbędnej wiedzy dla realizacji współfinansowanego przez Unię Europejską programu „Uczelnia dostępna”, którego Politechnika Śląska (miejsce pracy autorek) jest beneficjentem, oraz szeroko pojętych działań edukacyjnych. Przyjęta dla prowadzenia badań metoda to w głównej mierze Post-Occupancy Evaluation (POE), która służy głównie do prawidłowego przeprowadzenia procesu programowania funkcjonalno-przestrzennego z wykorzystaniem badań literaturowych, eksperckich i partycypacyjnych. Podstawową ideą podjętych dociekań jest zasada Universal Design.
    As regards the introduction and implementation of the "Accessibility Plus" Act in Poland, a considerable interest in universal design has been noted, with a simultaneously low level of awareness of the needs of people with disabilities. The article presents selected studies and its conclusions in the area of application of architectural and urban planning accessibility for people with disabilities in the cities of the Upper Silesia Region (Poland). The aim of the permanent research conducted by the Authors of the text is, first of all, to gather the necessary knowledge for the execution of the "Accessible University" co-financed by the European Union, of which the Silesian University of Technology (the place of work of the Authors) is the beneficiary, and on the other hand, educational activities. The Post-Occupancy Evaluation (POE) method, which serves primarily to properly perform the functional-spatial programming process, using literature, expert and participatory research, has been mainly adopted for the research.
    Źródło:
    Builder; 2021, 25, 4; 56-58
    1896-0642
    Pojawia się w:
    Builder
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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