- Tytuł:
- Cytotoxic Effects of Different ICG Concentrations and Laser Parameters on Neuroblastoma
- Autorzy:
-
Ak, A.
Kaya, Ö.
Coşan, D.
Gülsoy, M. - Powiązania:
- https://bibliotekanauki.pl/articles/1402428.pdf
- Data publikacji:
- 2015-08
- Wydawca:
- Polska Akademia Nauk. Instytut Fizyki PAN
- Tematy:
-
42.62.-B
87.19.XJ - Opis:
- Photodynamic therapy (PDT) is a minimally invasive treatment for cancer therapy. It can be administered in combination with other treatments such as chemotherapy, radiotherapy, and surgical excision. PDT involves a photosensitizing agent that is activated by exposure to a specific wavelength of light. PDT is a cold photochemical process, there is no tissue heating. In our study, we investigated whether different laser parameters with different concentrations of indocyanine green (ICG) have cytotoxic and anti-proliferative effects on neuroblastoma. Plates were divided groups as control, only ICG concentrations (25 and 50 μg/ml), only laser treatment I (50 J/cm²), only laser treatment II (100 J/cm²), 25 μg/ml ICG + laser treatment I and 25 μg/ml ICG + laser treatment II, 50 μg/ml ICG + laser treatment I and 50 μg/ml ICG + laser treatment II. Neuroblastoma cell lines were irradiated with an in-house developed diode laser system (λ=809 nm, 70 mW/cm², 50 & 100 J/cm²) in continuous wave operation mode after ICG application. Cell proliferation was measured by XTT assay after light irradiation. Cell proliferation was decreased in a dose-dependent manner in 25 and 50 μg/ml ICG concentrations when compared with control. The applied ICG concentrations (especially 50 μg/ml) had cytotoxic effects for neuroblastoma cell lines, SH-SY5Y. There was no difference between laser treatment groups (L 50 & 100 J/cm²). However, PDT groups (laser exposure with ICG) showed significant inhibition of cell viability (p<0.05). Additionally, laser exposure did not increase the well temperature above the incubation parameter. In conclusion, PDT has cytotoxic effects in neuroblastoma cell lines. Appropriate ICG dose - laser parameter combinations must be determined for each cell type. Different energy densities may cause different effects of PDT on inhibition of cell viability.
- Źródło:
-
Acta Physica Polonica A; 2015, 128, 2B; B-381-B-383
0587-4246
1898-794X - Pojawia się w:
- Acta Physica Polonica A
- Dostawca treści:
- Biblioteka Nauki
Artykuł