- Tytuł:
- Sulforaphane-mediated induction of a phase 2 detoxifying enzyme NAD(P)H:quinone reductase and apoptosis in human lymphoblastoid cells.
- Autorzy:
-
Misiewicz, Irena
Skupińska, Katarzyna
Kowalska, Elżbieta
Lubiński, Jan
Kasprzycka-Guttman, Teresa - Powiązania:
- https://bibliotekanauki.pl/articles/1041549.pdf
- Data publikacji:
- 2004
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
cancer chemoprevention
sulforaphane
lymphoblastoid cells - Opis:
- The effect of sulforaphane on human lymphoblastoid cells originating from a patient of a high cancer risk was studied. Sulforaphane (SFN) is a naturally occurring substance of chemopreventive activity. In our study, changes in cell growth, induction of apoptosis and phase 2 enzymes as well as glutathione level were examined. Apoptosis was tested by confocal microscopy at three stages: change in mitochondrial membrane potential, caspase activation and phosphatidylserine externalization. We show that SFN increases the activity of the detoxification system: it increases quinone reductase activity at low concentration (0.5-1 μM) and raises glutathione level in a dose-dependent manner. At higher doses (2.5-10 μM) sulforaphane is a cell growth modulator, as it caused cell growth cessation (IC50 = 3.875 μM), and apoptosis inducer. The results obtained suggest that sulforaphane acts as a chemopreventive agent in human lymphoblastoid cells.
- Źródło:
-
Acta Biochimica Polonica; 2004, 51, 3; 711-721
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł