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Wyświetlanie 1-3 z 3
Tytuł:
Division of methods for counting helminths’ eggs and the problem of efficiency of these methods
Autorzy:
Jaromin-Gleń, Katarzyna
Kłapeć, Teresa
Łagód, Grzegorz
Karamon, Jacek
Malicki, Jacek
Skowrońska, Agata
Bieganowski, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/988990.pdf
Data publikacji:
2017
Wydawca:
Instytut Medycyny Wsi
Tematy:
helminths’ eggs
parasitological methods
sewage sludge
biosolid matrices
efficiency of method
Opis:
From the sanitary and epidemiological aspects, information concerning the developmental forms of intestinal parasites, especially the eggs of helminths present in our environment in: water, soil, sandpits, sewage sludge, crops watered with wastewater are very important. The methods described in the relevant literature may be classified in various ways, primarily according to the methodology of the preparation of samples from environmental matrices prepared for analysis, and the sole methods of counting and chambers/instruments used for this purpose. In addition, there is a possibility to perform the classification of the research methods analyzed from the aspect of the method and time of identification of the individuals counted, or the necessity for staining them. Standard methods for identification of helminths’ eggs from environmental matrices are usually characterized by low efficiency, i.e. from 30% to approximately 80%. The efficiency of the method applied may be measured in a dual way, either by using the method of internal standard or the ‘Split/Spike’ method. While measuring simultaneously in an examined object the efficiency of the method and the number of eggs, the ‘actual’ number of eggs may be calculated by multiplying the obtained value of the discovered eggs of helminths by inverse efficiency.
Źródło:
Annals of Agricultural and Environmental Medicine; 2017, 24, 1
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparison of the efficiency of two commercial kits - ELFA and Western blot in estimating the phase of Toxoplasma gondii infection in pregnant women
Autorzy:
Sroka, Jacek
Wójcik-Fatla, Angelina
Zając, Violetta
Sawczyn, Anna
Cisak, Ewa
Karamon, Jacek
Dutkiewicz, Jacek
Bojar, Iwona
Powiązania:
https://bibliotekanauki.pl/articles/991156.pdf
Data publikacji:
2016
Wydawca:
Instytut Medycyny Wsi
Tematy:
toxoplasmosis
pregnant women
seroprevalence
antibodies
igg
igm
elfa
western blot
avidity
correlation of serologic tests
Opis:
Sera of 89 pregnant women were selected according to the results of ELFA IgM, IgG and avidity IgG, and tested with commercial tests IgM, IgG and avidity IgG Western Blot (WB) to compare the efficacy of both techniques in determining the phase of T. gondii infection. In total, 81 of 89 tested sera (91.0%) were classified as positive, both in the ELFA and WB tests for the presence of anti-Toxoplasma antibodies of class IgG, indicating a past infection, while the prevalence of anti-Toxoplasma positive reactions associated with the antibodies of class IgM indicating a recent infection was much lower – 31.5% and 20.2%, respectively. Sera of 81 women were also tested in the ELFA and WB tests for avidity, e.g. ability of forming high-molecular IgG antibody complexes. Low or medium results in these tests (in this study all classified as low), indicating a recent infection, were detected by ELFA and WB in 22.2% and 45.7% of the total examined samples, respectively. The Spearman’s rank test for correlation, performed for recognition of quantitative data of the ELFA and WB tests (index, units or points), revealed a highly significant correlation between the ELFA and WB tests for homologous classes of antibodies, both for IgM and IgG (p<0.00001). In contrast, the ELFA and WB tests for detection of anti-Toxoplasma IgM antibodies were not correlated with the ELFA and WB tests for detection of anti-Toxoplasma IgG antibodies (p>0.05), except for the WB test for IgM antibodies, which showed a significant correlation with the ELFA test for IgG antibodies (p<0.01). A highly significant negative correlation between the ELFA and WB test for IgM antibodies and ELFA and WB tests for IgG avidity was demonstrated (p<0.01), except for a relationship between the WB test for IgM and WB for avidity, which was not significant. Such negative correlations are theoretically expected, as strong complexes with the participation of IgG antibodies are absent in the early phase of toxoplasmosis when early antibodies of IgM class are present. Summarizing, this study indicates the high usefulness of the commercial ELFA and WB tests in serodiagnostics of toxoplasmosis in pregnant women. Special attention should be paid to parallel detection of IgM antibodies and low values in the ELFA and WB tests for IgG avidity, which indicates a recent infection which may be associated with a clinical form of congenital toxoplasmosis and damage to the foetus.
Źródło:
Annals of Agricultural and Environmental Medicine; 2016, 23, 4
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Sytuacja epidemiologiczna włośnicy w Polsce - I półrocze 2022 r.
Epidemiology of trichinellosis in Poland - the first half of 2022
Autorzy:
Bełcik, Aneta
Korpysa-Dzirba, Weronika
Bilska-Zając, Ewa
Różycki, Mirosław
Gontarczyk, Aneta
Kochanowski, Maciej
Samorek-Pieróg, Małgorzata
Karamon, Jacek
Cencek, Tomasz
Powiązania:
https://bibliotekanauki.pl/articles/22443123.pdf
Data publikacji:
2022
Wydawca:
Krajowa Izba Lekarsko-Weterynaryjna
Tematy:
dziki
sytuacja epidemiologiczna
rok 2022
zwierzęta dziko żyjące
zwierzęta łowne
choroby zwierząt
choroby pasożytnicze
włośnica
występowanie
trichinellosis
wild boars
hunting animals
Polska
Opis:
The aim of this study was to present the current situation of trichinellosis, basing on the results of examination of the tissues of animals hunted in Poland. Investigation was carried out from 1st January to 30th June 2022 in the Department of Parasitology and Parasitic Diseases of National Veterinary Research Institute in Poland. Samples were collected out of 103 wild boars (Sus scrofa) and one wolf (Canis lupus), originating from 14 voivodeships. The study of wild boars muscle tissues revealed 89 positive samples, including 60 T. spiralis, 21 T. britovi, two T. pseudospiralis, and five mixed invasions with T. spiralis and T. britovi. In one case no amplification products were obtained. T. britovi was also found in the sample of wolf muscle tissue. This study emphasize the fact that trichinellosis in hunting animals is still a zoonotic threat, and should be closely monitored.
Źródło:
Życie Weterynaryjne; 2022, 97, 09; 607-610
0137-6810
Pojawia się w:
Życie Weterynaryjne
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-3 z 3

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