Autor: Kamel, Katarzyna - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Application of the High Resolution Melting analysis for genetic mapping of Sequence Tagged Site markers in narrow-leafed lupin (Lupinus angustifolius L.)
Autorzy:: Kamel, Katarzyna
Kroc, Magdalena
Święcicki, Wojciech
Powiązania:: https://bibliotekanauki.pl/articles/1039001.pdf
Data publikacji:: 2015
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: HRM
molecular markers
genotyping
narrow-leafed lupin
Opis:: Sequence tagged site (STS) markers are valuable tools for genetic and physical mapping that can be successfully used in comparative analyses among related species. Current challenges for molecular markers genotyping in plants include the lack of fast, sensitive and inexpensive methods suitable for sequence variant detection. In contrast, high resolution melting (HRM) is a simple and high-throughput assay, which has been widely applied in sequence polymorphism identification as well as in the studies of genetic variability and genotyping. The present study is the first attempt to use the HRM analysis to genotype STS markers in narrow-leafed lupin (Lupinus angustifolius L.). The sensitivity and utility of this method was confirmed by the sequence polymorphism detection based on melting curve profiles in the parental genotypes and progeny of the narrow-leafed lupin mapping population. Application of different approaches, including amplicon size and a simulated heterozygote analysis, has allowed for successful genetic mapping of 16 new STS markers in the narrow-leafed lupin genome.
Źródło:: Acta Biochimica Polonica; 2015, 62, 3; 533-540
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Colorimetric Vs. Chromatographic Analyses of Alkaloids in Lupin Seeds
Autorzy:: Wilczura, Paulina
Święcicki, Wojciech
Kamel, Katarzyna
Wasiak, Wiesław
Powiązania:: https://bibliotekanauki.pl/articles/2199704.pdf
Data publikacji:: 2018-12-20
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Tematy:: gas chromatography
Lupinus angustifolius L.;
Lupinus albus L.;
sweet/bitter lupins;
Wagner’s colorimetric test
Opis:: A characteristic trait of lupins is a production of alkaloids, which are a toxic and bitter taste compound ofseeds. Due to the lack of fast, sensitive and inexpensive screening techniques to identify and reject high alkaloidplant material, development of suitable tools is important challenges for lupins breeding and seed production.The aim of this study was to compare two alkaloid content estimation methods in Lupinus angustifoliusL. and Lupinus albus L.During the Wagner’s colorimetric test, which is recommended by the UPOV, seed halves were stained onfour colors depending on the alkaloid content but only the level of 0.5% – 0.6% showed clear color change.Gas chromatography allowed accurate quantification and qualification of alkaloid content.Since safe alkaloid content for consumption is 0.02% of seed dry weight, colorimetric method is less usefulfor dividing lupin cultivars into sweet and bitter, than gas chromatography but can be used as a screeningtechnique.
Źródło:: Plant Breeding and Seed Science; 2018, 78; 63-67
1429-3862
2083-599X
Pojawia się w:: Plant Breeding and Seed Science
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Plant expression systems for production of hemagglutinin as a vaccine against influenza virus
Autorzy:: Redkiewicz, Patrycja
Sirko, Agnieszka
Kamel, Katarzyna
Góra-Sochacka, Anna
Powiązania:: https://bibliotekanauki.pl/articles/1039262.pdf
Data publikacji:: 2014
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: recombinant hemagglutinin
avian influenza
plant-produced
VLP
ELISA
Opis:: Many examples of a successful application of plant-based expression systems for production of biologically active recombinant proteins exist in the literature. These systems can function as inexpensive platforms for the large scale production of recombinant pharmaceuticals or subunit vaccines. Hemagglutinin (HA) is a major surface antigen of the influenza virus, thus it is in the centre of interests of various subunit vaccine engineering programs. Large scale production of recombinant HA in traditional expression systems, such as mammalian or insect cells, besides other limitations, is expensive and time-consuming. These difficulties stimulate an ever-increasing interest in plant-based production of this recombinant protein. Over the last few years many successful cases of HA production in plants, using both transient and stable expression systems have been reported. Various forms of recombinant HA, including monomers, trimers, virus like particles (VLPs) or chimeric proteins containing its fusion with other polypeptides were obtained and shown to maintain a proper antigenicity. Immunizations of animals (mice, ferrets, rabbits or chickens) with some of these plant-derived hemagglutinin variants were performed, and their effectiveness in induction of immunological response and protection against lethal challenge with influenza virus demonstrated. Plant-produced recombinant subunit vaccines and plant-made VLPs were successfully tested in clinical trials (Phase I and II) that confirmed their tolerance and immunogenicity.
Źródło:: Acta Biochimica Polonica; 2014, 61, 3; 551-560
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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