Autor: KARAŚ, Magdalena - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Localization of the attachment site of oligoglucans to Mesorhizobium loti HAMBI 1148 murein
Autorzy:: Karaś, Magdalena
Russa, Ryszard
Powiązania:: https://bibliotekanauki.pl/articles/1040650.pdf
Data publikacji:: 2009
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: oligoglucan
murein
MALDI-TOF
deamination
Mesorhizobium
Opis:: The location and nature of the linkage between peptidoglycan and oligoglucans in the cell wall of Mesorhizobium loti HAMBI 1148 have been defined by the analysis of nitrous acid deamination of peptidoglycan glucosaminyl residues. The MurNH2-Glcn fraction was obtained after converting deaminoacylated and N-deacetylated muramyl residues in the cell wall preparation to lactam forms which were stable during subsequent deamination, followed by reduction and opening of the lactams. GC/MS analysis of this material, subjected to partial hydrolysis and reduction or to methanolysis followed by peracetylation, confirmed the presence of glucosyl residues glycosidically attached to muramic acid. The MALDI-TOF spectroscopic analysis of the deaminated material also revealed the presence of [M-H]- or [M+Na-2H]- ions representing fragments containing muramic acid with one to three linked glucose residues. The analysis of fully methylated neutral oligosaccharides released from the peptidoglycan with lysozyme followed by borohydride reduction showed the presence of di- and trisaccharides lacking the reducing end.
Źródło:: Acta Biochimica Polonica; 2009, 56, 1; 155-160
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Occurrence of New Polyenoic Very Long Chain Acyl Residues in Lipids from Acanthamoeba castellanii
Autorzy:: PALUSIŃSKA-SZYSZ, Marta
TURSKA-SZEWCZUK, Anna
KARAŚ, Magdalena
RUSSA, Ryszard
DROŻAŃSKI, Wincenty J.
Powiązania:: https://bibliotekanauki.pl/articles/763712.pdf
Data publikacji:: 2009
Wydawca:: Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
Tematy:: Acanthamoeba castellanii, endoparasites, long chain polyenoic fatty acids, double bond position, dimethyloxazoline derivatives, dimetyldisulfide adducts, non- methylene interrupted fatty acids
Opis:: The cellular fatty acid composition of Acanthamoeba castellanii, a unicellular bacteriovorous organism, was reinvestigated. Lipids from amoebae grown axenically in proteose peptone-yeast extract-glucose medium were extracted with chloroform–methanol and separated by silicic acid column chromatography into non-polar and polar fractions. The fatty acid composition of the lipids and the double-bond position of the unsaturated acids have been determined by capillary gas chromatography-mass spectrometry (GC-MS) of their corresponding methyl esters, 2-alkenyl-4,4-dimethyloxazoline (DMOX) derivatives and dimethyldisulfide (DMDS) adducts. Evidence is given that lipids from A. castellanii in addition to the three already identified saturated straight chain fatty acids: tetradecanoic (C14:0), hexadecanoic (C16:0), octadecanoic (C18:0), and six preponderant unsaturated fatty acids: hexadecenoic (C16:1 Δ7), octadecenoic (C18:1 Δ9), octadecadienoic (C18:2 Δ9,12), eicosadienoic (C20:2 Δ11,14), eicosatrienoic (C20:3 Δ8,11,14), and eicosatetraenoic (C20:4 Δ5,8,11,14), contain additionally four very long chain unsaturated fatty acids: octacosenoic (C28:1 Δ21), octacosadienoic (C28:2 Δ5,21), triacontadienoic (30:2 Δ21,24), and triacontatrienoic (C30:3 Δ5,21,24) previously unreported in lipids of A. castellanii. These new long chain fatty acids account for approximately 25% of total fatty acids. To our knowledge, this is the first report of very long chain polyenoic fatty acids present in lipids extracted from A. castellanii cells.
Źródło:: Acta Protozoologica; 2009, 48, 1
1689-0027
Pojawia się w:: Acta Protozoologica
Dostawca treści:: Biblioteka Nauki