- Tytuł:
- Methyl jasmonate, yeast extract and sucrose stimulate phenolic acids accumulation in Eryngium planum L. shoot cultures
- Autorzy:
-
Kikowska, Małgorzata
Kędziora, Izabela
Krawczyk, Aldona
Thiem, Barbara - Powiązania:
- https://bibliotekanauki.pl/articles/1039089.pdf
- Data publikacji:
- 2015
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
chlorogenic acid
elicitation
flat sea holly
in vitro shoot culture
rosmarinic acid - Opis:
- Eryngium planum L. has been reported as a medicinal plant used in traditional medicine in Europe. The tissue cultures may be an alternative source of the biomass rich in desired bioactive compounds. The purpose of this study was to investigate the influence of the biotechnological techniques on the selected phenolic acids accumulation in the agitated shoot cultures of E. planum. Qualitative and quantitative analyses of those compounds in 50% aqueous - methanolic extracts from the biomass were conducted by applying the HPLC method. Methyl jasmonate (MeJA), yeast extract (YE) and sucrose (Suc) stimulated accumulation of the phenolic acids: rosmarinic (RA), chlorogenic (CGA) and caffeic (CA) in in vitro shoot cultures. Cultivation of shoots in liquid MS media supplemented with 1.0 mg L-1 6-benzyladenine and 0.1 mg L-1 indole-3-acetic acid in the presence of 100 µM MeJA for 48h was an optimum condition of elicitation and resulted in approximately 4.5-fold increased content of RA + CGA + CA in plant material compared to the control (19.795 mg g-1 DW, 4.36 mg g-1 DW, respectively). The results provide the first evidence that the selected phenolic acids can be synthesized in elicited shoot cultures of flat sea holly in higher amount than in untreated shoots.
- Źródło:
-
Acta Biochimica Polonica; 2015, 62, 2; 197-200
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł