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Wyszukujesz frazę "Jarmołowski, Artur" wg kryterium: Autor

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    Wyświetlanie 1-10 z 10
    Tytuł:
    The nuclear cap-binding protein complex is not essential for nonsense-mediated mRNA decay (NMD) in plants
    Autorzy:
    Dzikiewicz-Krawczyk, Agnieszka
    Piontek, Paulina
    Szweykowska-Kulińska, Zofia
    Jarmołowski, Artur
    Powiązania:
    https://bibliotekanauki.pl/articles/1040693.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    nuclear cap-binding protein complex
    alternative splicing
    mRNA surveillance
    NMD
    premature terminatin codon
    nonsense-mediated mRNA decay
    Opis:
    In this study we investigated whether in plants, like in mammals, components of the nuclear cap-binding protein complex (CBC) are involved in nonsense-mediated mRNA decay (NMD). We selected several genes producing at least two alternatively spliced mRNA variants: one with a premature termination codon (PTC+) and another without it (PTC-). For each gene the PTC+/PTC- ratio was calculated using RT-PCR and direct sequencing in four Arabidopsis thaliana lines: wild type, the NMD mutant atupf3-1 and two CBC mutants: cbp20 and abh1. Whereas in the NMD mutant the ratios of PTC+/PTC- splice variants were higher than in wild-type plants, the two CBC mutants investigated showed no change in the PTC+/PTC- ratios. Our results suggest that neither CBP20 nor CBP80 is involved in NMD in A. thaliana.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 4; 825-828
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    RNA interference and its role in the regulation of eucaryotic gene expression.
    Autorzy:
    Szweykowska-Kulińska, Zofia
    Jarmołowski, Artur
    Figlerowicz, Marek
    Powiązania:
    https://bibliotekanauki.pl/articles/1043669.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    gene silencing
    small RNAs
    RNAi
    PTGS
    quelling
    Opis:
    Several years ago it was discovered that plant transformation with a transcribed sense transgene could shut down the expression of a homologous endogenous gene. Moreover, it was shown that the introduction into the cell of dsRNA (double-stranded RNA) containing nucleotide sequence complementary to an mRNA sequence causes selective degradation of the latter and thus silencing of a specific gene. This phenomenon, called RNA interference (RNAi) was demonstrated to be present in almost all eukaryotic organisms. RNAi is also capable of silencing transposons in germ line cells and fighting RNA virus infection. Enzymes involved in this process exhibit high homology across species. Some of these enzymes are involved in other cellular processes, for instance developmental timing, suggesting strong interconnections between RNAi and other metabolic pathways. RNAi is probably an ancient mechanism that evolved to protect eukaryotic cells against invasive forms of nucleic acids.
    Źródło:
    Acta Biochimica Polonica; 2003, 50, 1; 217-229
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    MicroRNA biogenesis: Epigenetic modifications as another layer of complexity in the microRNA expression regulation
    Autorzy:
    Bhat, Susheel
    Jarmolowski, Artur
    Szweykowska-Kulińska, Zofia
    Powiązania:
    https://bibliotekanauki.pl/articles/1038729.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    microRNA
    microRNA biogenesis
    m6A modification
    m6A RNA methyltransferase
    Opis:
    Since their discovery, microRNAs have led to a huge shift in our understanding of the regulation of key biological processes. The discovery of epigenetic modifications that affect microRNA expression has added another layer of complexity to the already tightly controlled regulatory machinery. Modifications like uridylation, adenylation and RNA editing have been shown to have variable effects on miRNA biogenesis and action. Methylation of the N6 adenosine has been studied extensively in mRNA. Presence of the N6-methyl-adenosine (m6A) mark and its critical importance in miRNA biogenesis in animals adds to our understanding of the regulatory mechanisms, while its effect on miRNA biogenesis in plants is yet to be understood.
    Źródło:
    Acta Biochimica Polonica; 2016, 63, 4; 717-723
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of nuclear matrix attachment regions on transgene expression in tobacco plants.
    Autorzy:
    Nowak, Witold
    Gawłowska0, Magdalena
    Jarmołowski, Artur
    Augustyniak, Jacek
    Powiązania:
    https://bibliotekanauki.pl/articles/1044093.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    plant transformation
    matrix attachment region
    transgene expression
    Opis:
    Matrix attachment regions (MARs) are thought to participate in the organization and segregation of independent chromosomal loop domains. Although there are several reports on the action of natural MARs in the context of heterologous genes in transgenic plants, in our study we tested a synthetic MAR (sMAR) with the special property of unpairing when under superhelical strain, for its effect on reporter gene expression in tobacco plants. The synthetic MAR was a multimer of a short sequence from the MAR 3' end of the immunoglobulin heavy chain (IgH) enhancer. This sMAR sequence was used to flank the β-glucuronidase (GUS) reporter gene within the T-DNA of the binary vector pBI121. Vectors with or without the sMARs were then used to transform tobacco plants by Agrobacterium tumefaciens. Transgenic plants containing the sMAR sequences flanking the GUS gene exhibited higher levels of transgene expression compared with transgenic plants which lacked the sMARs. This effect was observed independently of the position of the sMAR at the 5' side of the reporter gene. However, variation of the detected transgene expression was significant in all transformed plant populations, irrespective of the construct used. Most genes whose expression has been studied in transgenic plants are generally expressed in appropriate patterns. However, transgene expression can vary within an extremely wide range, often showing only a very low level [1, 2]. Variation in transgene expression is frequently attributed to corresponding variation in the transcription potential of different chromosomal insertion sites. DNA sequences called scaffold/matrix attachment regions (S/MARs) are involved in the structural and functional organization of all eukaryotic genomes. Evolutionarily, the structures of these sequences seem to be conserved. Typically, S/MARs are located every 5 to 200 kb of sequence and are known to bind specifically to components of the nuclear scaffold, therefore suggesting that they are responsible for loop domain base formation [3, 4].Of the MAR elements reported, many do not display extensive sequence homology. It is therefore reasonable to assume that the scaffold probably recognizes some structural features of the MAR DNA rather than a specific sequence [5].MARs appear to be functionally conserved, since animal MARs can bind to plant nuclear scaffolds and vice versa [6, 7].Most MARs have been generally characterized as AT-rich sequences. However, AT-richness per se is not a sufficient criterion for specific sequence recognition of MARs by specific binding proteins [7]. Their capacity to bind to the nuclear matrix is determined by the specific structure of DNA. A prominent structural characteristic of different MARs is their strong potential for extensive unpairing when subjected to superhelical strain [8, 9]. The ability to assume a stably unpaired conformation has been described for several MARs. For example, within the MAR 3' end of immunoglobulin heavy chain (IgH) enhancer there is an AATATATTT motif that is a nucleation site for DNA unwinding [10]. Concatamerized oligonucleotides containing seven repeats of this sequence exhibited a strong affinity for the nuclear scaffold and increased SV40 promoter activity in stably transformed mouse cells [11].In this paper we present results of our studies that concern the effect of a synthetic MAR on transgene expression in tobacco plants. The synthetic MAR sequences were used to flank the β-glucuronidase (GUS) gene whose transcription was under control of the 35S CaMV promoter in the binary vector pBI121. This construct was introduced into tobacco plants and the GUS reporter gene expression was monitored in stably transformed plants.
    Źródło:
    Acta Biochimica Polonica; 2001, 48, 3; 637-646
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Developmental changes in barley microRNA expression profiles coupled with miRNA target analysis
    Autorzy:
    Pacak, Andrzej
    Kruszka, Katarzyna
    Swida-Barteczka, Aleksandra
    Nuc, Przemyslaw
    Karlowski, Wojciech
    Jarmolowski, Artur
    Szweykowska-Kulinska, Zofia
    Powiązania:
    https://bibliotekanauki.pl/articles/1038742.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    barley
    microRNA
    deep sequencing
    development
    Opis:
    MicroRNAs are 19- to 24-nt-long single-stranded RNAs that are crucial regulators of gene expression which control plant development and response to environmental cues. We have analyzed microtranscriptomes of five barley developmental stages. Generally, during the barley development, miR168-3p and miR1432-5p levels increase while the 5'U-miR156-5p level decreases (with exception for the 2-week-old barley). We have identified two miR156-5p izomiRs (called 5'U-miR156-5p [20 nt] and 5'UU-miR156-5p [21 nt]), which were expressed differently during barley development. The 5' U-miR156-5p level decreased in 3-week-, 6-week-, and 68-day-old barley, when compared to the 1-week-old plants. Meanwhile, the 5' UU-miR156-5p level increased significantly in the 68-day-old barley plants. Moreover, only the 5' U-miR156 isomiR recognizes and guides unique transcription factor mRNAs from the Squamosa Promoter Binding Protein-Like (SPL) family. We identified many non-canonical microRNAs with changed expression levels during the barley development. Here, we present the profiles of microRNA expression characteristics for particular barley developmental stages. These analyses are accompanied by the experimental degradome analysis of miRNA targets.
    Źródło:
    Acta Biochimica Polonica; 2016, 63, 4; 799-809
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cloning and characterization of Arabidopsis thaliana AtNAP57 - a homologue of yeast pseudouridine synthase Cbf5p.
    Autorzy:
    Maceluch, Jarosław
    Kmieciak, Maciej
    Szweykowska-Kulińska, Zofia
    Jarmołowski, Artur
    Powiązania:
    https://bibliotekanauki.pl/articles/1043736.pdf
    Data publikacji:
    2002
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Cbf5p
    dyskerin
    H/ACA snoRNAs
    Y
    pseudouridine synthase
    NAP57
    Opis:
    Rat Nap57 and its yeast homologue Cbf5p are pseudouridine synthases involved in rRNA biogenesis, localized in the nucleolus. These proteins, together with H/ACA class of snoRNAs compose snoRNP particles, in which snoRNA guides the synthase to direct site-specific pseudouridylation of rRNA. In this paper we present an Arabidopsis thaliana protein that is highly homologous to Cbf5p (72% identity and 85% homology) and NAP57 (67% identity and 81% homology). Moreover, the plant protein has conserved structural motifs that are characteristic features of pseudouridine synthases of the TruB class. We have named the cloned and characterized protein AtNAP57 (A rabidopsis t haliana homologue of NAP57 ). AtNAP57 is a 565 amino-acid protein and its calculated molecular mass is 63 kDa. The protein is encoded by a single copy gene located on chromosome 3 of the A. thaliana genome. Interestingly, the AtNAP57 gene does not contain any introns. Mutations in the human DKC1 gene encoding dyskerin (human homologue of yeast Cbf5p and rat NAP57) cause dyskeratosis congenita a rare inherited bone marrow failure syndrome characterized by abnormal skin pigmentation, nail dystrophy and mucosal leukoplakia.
    Źródło:
    Acta Biochimica Polonica; 2002, 49, 3; 699-709
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Barley primary microRNA expression pattern is affected by soil water availability
    Autorzy:
    Swida-Barteczka, Aleksandra
    Kruszka, Katarzyna
    Grabowska, Aleksandra
    Pacak, Andrzej
    Jarmolowski, Artur
    Kurowska, Marzena
    Szarejko, Iwona
    Szweykowska-Kulinska, Zofia
    Powiązania:
    https://bibliotekanauki.pl/articles/1038746.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    pri-miRNA
    miRNA
    drought
    rehydration
    barley genotypes
    Opis:
    MicroRNAs are short molecules of 21-24 nt in length. They are present in all eukaryotic organisms and regulate gene expression by guiding posttranscriptional silencing of mRNAs. In plants, they are key players in signal transduction, growth and development, and in response to abiotic and biotic stresses. Barley (Hordeum vulgare) is an economically important monocotyledonous crop plant. Drought is the world's main cause of loss in cereal production. We have constructed a high-throughput Real-Time RT-qPCR platform for parallel determination of 159 barley primary microRNAs' levels. The platform was tested for two drought-and-rehydration-treated barley genotypes (Rolap and Sebastian). We have determined changes in the expression of primary microRNAs responding to mild drought, severe drought, and rehydration. Based on the results obtained, we conclude that alteration in the primary microRNA expression is relative to the stress's intensity. Mild drought and rehydration mostly decrease the pri-miRNA levels in both of the tested genotypes. Severe drought mainly induces the primary microRNA expression. The main difference between the genotypes tested was a much-stronger induction of pri-miRNAs in Rolap encountering severe drought. The primary microRNAs respond dynamically to mild drought, severe drought, and rehydration treatments. We propose that some of the individual pri-miRNAs could be used as drought stress or rehydration markers. The usage of the platform in biotechnology is also postulated.
    Źródło:
    Acta Biochimica Polonica; 2016, 63, 4; 817-824
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Arabidopsis thaliana microRNA162 level is posttranscriptionally regulated via splicing and polyadenylation site selection
    Autorzy:
    Barciszewska-Pacak, Maria
    Knop, Katarzyna
    Jarmołowski, Artur
    Szweykowska-Kulińska, Zofia
    Powiązania:
    https://bibliotekanauki.pl/articles/1038744.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    miRNA
    pri-miRNA
    abiotic stress
    gene expression
    Opis:
    Arabidopsis microRNA162 (miRNA162) level regulation was studied under abiotic stresses, such as drought and salinity. The TaqMan® microRNA assay proved that A. thaliana miRNA162 level was elevated under these stresses, confirming its salt and drought responsiveness. The promoter region analyses of A. thaliana miRNA162a and b genes (MIR162a and MIR162b) identified numerous salinity and drought responsive elements. However, our results indicated that Arabidopsis MIR162a was presumably the main locus responsible for the mature ath-miRNA162 accumulation under the stresses tested, and the MIR162b was generally rather weakly expressed, both in control and under the stress conditions. The MIR162a structure was confirmed to be complex and the pri-miRNA162a hairpin structure was shown to span an alternative exon and an intron. The MIR162a transcription generated a few pri-miRNA162a splicing isoforms that could be functional and non-functional. Upon drought and salinity stresses, the regulation of the pri-miRNA162a alternative splicing pattern revealed an increase of a functional pri-miR162a isoform and a preferential distal polyA site selection under the stress conditions. Apart from the potential transcriptional regulation of the miRNA genes (MIRs) expression, the data obtained point to an essential role of posttranscriptional regulation of Arabidopsis microRNA162 level.
    Źródło:
    Acta Biochimica Polonica; 2016, 63, 4; 811-816
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Analysis of potyvirus terminal protein VPg-transgenic Arabidopsis thaliana plants
    Autorzy:
    Wojtal, Izabela
    Piontek, Paulina
    Grzela, Renata
    Jarmołowski, Artur
    Zagórski, Włodzimierz
    Chroboczek, Jadwiga
    Powiązania:
    https://bibliotekanauki.pl/articles/1039885.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    VPg protein
    Potyvirus
    pathogen-derived resistance
    transgenic plant
    Opis:
    Virus-coded VPg protein of Potato virus Y (PVY) does not have homologs apart from other VPgs. Since VPg is indispensable for the potyvirus life cycle, it appeared a good candidate for eliciting pathogen-derived resistance to PVY. Following agroinfection used to obtain PVY VPg-transgenic Arabidopsis thaliana plants, only few transgenic seeds were recovered giving rise to six transgenic plants that contained the VPg gene with the correct sequence. They generated VPg mRNA, but VPg protein was not detected. Some plants were immune to PVY infection suggesting post-transcriptional gene silencing. However, the likely PVY VPg toxicity exerted at an early stage of transformed seeds development precludes its use for engineering pathogen-derived resistance.
    Źródło:
    Acta Biochimica Polonica; 2011, 58, 3; 349-353
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Abscisic acid does not influence the subcellular distribution of the HYL1 protein from Arabidopsis thaliana
    Autorzy:
    Lesicka-Górecka, Joanna
    Szarzyńska, Bogna
    Sawczak, Marta
    Bagdiul, Ivona
    Górski, Paweł
    Jarmołowski, Artur
    Szweykowska-Kulińska, Zofia
    Powiązania:
    https://bibliotekanauki.pl/articles/1040709.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    subcellular distribution
    abscisic acid
    Arabidopsis thaliana
    expression profile
    HYL1 protein
    Opis:
    HYL1 is a nuclear protein involved in the processing of miRNAs but its exact function remains unknown. Arabidopsis thaliana hyl1 mutants exhibit hypersensitivity to ABA. We decided to answer the question whether ABA affects the HYL1 protein localization within the cell and show that it does not. We also studied the expression of HYL1 in different tissues and organs. In this paper we show for the first time the expression profile of the HYL1 protein using anti-HYL1 antibodies. The protein is present in seedlings and mature plants in all organs studied, with the highest amount in inflorescences. A. thaliana HYL1 protein has several repetitions of a 28-amino-acid sequence at the C-terminus that confer protein instability. Our bioinformatic analysis of HYL1 homologs in different Brassica species shows that this repetition is typical only for Arabidopsis. This may suggest a relatively late evolutionary acquisition of the C-terminal domain.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 3; 517-524
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-10 z 10

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