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Wyszukujesz frazę "Jahromi, K.T." wg kryterium: Autor


Wyświetlanie 1-2 z 2
Tytuł:
Some brain peptides regulating the secretion of digestive enzymes in the Indian meal moth, Plodia interpunctella (Lepidoptera: Pyralidae)
Autorzy:
Sajjadian, S.M.
Hosseininaveh, V.
Jahromi, K.T.
Powiązania:
https://bibliotekanauki.pl/articles/66302.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The Indian meal moth, Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae) is a destructive polyphagous pest of many stored products. To interfere with the physiological processes, especially digestion, of the larval pest, more information on the regulatory mechanisms is needed. The brain extract from 1-day-old last instar larvae of P. interpunctella was examined. In the bioassays, the midguts were treated with the brain extract, and the carbohydrase and protease activities were measured. The brain extract showed increasing dose-dependent effects on α-amylase, α-glucosidase, β-glucosidase, α-galactosidase, β-galactosidase, and trypsin secretion in the larval midgut. The extract was further characterised and partially purified using high performance liquid chromatography (HPLC). Several peptides were determined in the brain extract regulating hydrolase activities in the larval midgut of the pest.
Źródło:
Journal of Plant Protection Research; 2014, 54, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of specific inhibitors on the gene expression of a digestive trypsin in Pieris brassicae L. (Lepidoptera: Pieridae)
Autorzy:
Sharifloo, A.
Zibaee, A.
Sendi, J.J.
Jahromi, K.T.
Powiązania:
https://bibliotekanauki.pl/articles/66407.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
specific inhibitor
gene expression
protein
digestion
trypsin
Pieris brassicae
Lepidoptera
Pieridae
Opis:
Protein digestion in insects relies on several groups of proteases, among which trypsin plays a prominent role. In the current study, larvae of Pieris brassicae L. were fed radish leaves treated with 1 mM concentrations of three specific inhibitors of trypsin: AEBSF.HCl [4-(2- -aminoethyl)-benzenesulfonyl fluoride, monohydrochloride], TLCK (N-a-tosyl-l-lysine chloromethyl ketone) and SBTI (Soybean Trypsin Inhibitor) to find their potential effects on gene expression of trypsin. Initially, RT-PCR analysis revealed a gene of 748 bp responsible for synthesizing the digestive trypsin in P. brassicae larvae. Also, qRT-PCR data indicated a statistically greater expression of trypsin gene in the larvae fed 1 mM concentrations of AEBSF.HCl, TLCK and SBTI than the control. Results of the current study indicated that synthetic inhibitors can not only negatively affect the gene expression of P. brassicae trypsin, but also the insect can activate a compensatory mechanism against interruption of protein digestion by inducing more expression of the gene and producing more trypsin into the midgut lumen.
Źródło:
Journal of Plant Protection Research; 2018, 58, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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