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Wyszukujesz frazę "Jędrzejczak, Renata" wg kryterium: Autor


Wyświetlanie 1-2 z 2
Tytuł:
Lead and cadmium content in wheat grain from different regions of Poland and risk analysis
Autorzy:
Jędrzejczak, Renata
Ręczajska, Wiesława
Powiązania:
https://bibliotekanauki.pl/articles/2174347.pdf
Data publikacji:
2022
Wydawca:
Instytut Technologiczno-Przyrodniczy
Tematy:
contamination
Polska
risk analysis
excessive content
toxic metals
wheat
Opis:
The level of wheat grain contamination with lead and cadmium was determined using electrothermal atomic absorption spectrometry with Zeeman background correction (ETAAS) after microwave digestion. The obtained limits of quantification were 0.001 mg∙kg-1, for both metals. A total of 300 samples of wheat grain from agricultural regions of Poland were examined, 150 each from the two consecutive harvest years 2017 and 2018. None of the tested samples exceeded the maximum level of these metals, as specified in the European regulations. The contents of lead and cadmium in wheat grain from both years of harvest ranged from <0.001 to 0.098 mg∙kg-1 and from 0.006 to 0.098 mg∙kg-1, respectively. Despite similar ranges of these metals, the highest lead contents were two times lower than the maximum limit value, while the highest cadmium contents were close to it. As for lead, a significantly higher (p < 0.05) mean content of this metal was found in wheat grain from the 2018 harvest compared to 2017 and in the western compared to eastern regions of the country. However, the cadmium contents did not differ significantly between the two harvest years, but were significantly higher (p < 0.05) in wheat grain from the southern regions compared to northern regions of Poland. Additionally, the highest contents of cadmium, close to the maximum limit, were found in the South-West region and in the both years of harvest. The risk analysis of the occurrence of the excessive contents of toxic metals in wheat grain showed a low risk level for lead in all investigated regions, and a medium level for cadmium, in general.
Źródło:
Journal of Water and Land Development; 2022, 55; 192--199
1429-7426
2083-4535
Pojawia się w:
Journal of Water and Land Development
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Intra-strains diversity of expression of polymorphic PKS4 gene in comparison in zearalenone production by Fusarium graminearum during in vitro cultivation
Autorzy:
Misiewicz, Anna
Goncerzewicz, Anna
Jędrzejczak, Renata
Zdziennicki, Filip
Powiązania:
https://bibliotekanauki.pl/articles/1038847.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Fusarium sp. PKS4
ZEA
gene expression
polymorphism
Opis:
Filamentous fungi belonging to the Fusarium genus are responsible for large economic losses due to their high pathogenicity and toxigenicity. Fusarium sp. may produce variety of mycotoxins, one of them is zearalenone (ZEA). The presence of the PKS4 gene shows the possibility of zearalenone biosynthesis by Fusarium sp. In this study, in four Fusarium graminearum and one Fusarium poae strains the presence of PKS4 genes and ZEA concentrations were determined. The presence of the PKS4 gene was confirmed by classical polymerase chain reaction (PCR) in three of four strains of F. graminearum. One strain with no PKS4 gene detected was found while still producing ZEA. In the present study, a real-time PCR assay has been successfully performed for the relative expression of Fusarium strains based on new designed primers targeting the PKS4 gene involved in ZEA biosynthesis. Result shows that P56/4 strain of F. graminearum has the highest mRNA level, in the range of 12, what correlates to the high production of this mycotoxin. In this study, a real-time PCR assay has been successfully developed for the prediction of the production of ZEA by F. graminearum strains by PCR real-time techniques based on primers targeting the gene, PKS4, involved in ZEA biosynthesis. The special significance was pointed to occurring genes polymorphism.
Źródło:
Acta Biochimica Polonica; 2016, 63, 1; 97-102
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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