Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Wyszukujesz frazę "Huang, K." wg kryterium: Autor


Tytuł:
A Precise and High Speed Charge-Pump PLL Model Based on SystemC/SystemC-AMS
Autorzy:
Ma, K.
Van Leuken, R.
Vidojkovic, M.
Romme, J.
Rampu, S.
Pflug, H.
Huang, L.
Dolmans, G.
Powiązania:
https://bibliotekanauki.pl/articles/227120.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
SystemC/SystemC-AMS
phase locked loop PLL
radio frequency
mixed-signal modeling
hardware description language
Opis:
The Phase Locked Loop (PLL) has become an important part of electrical systems. When designing a PLL, an efficient and reliable simulation platform for system evaluation is needed. However, the closed loop simulation of a PLL is time consuming. To address this problem, in this paper, a new PLL model containing both digital and analog parts based on SystemC/SystemC-AMS (BETA version) is presented. Many imperfections such as Voltage Control Oscillator (VCO) noise or reference jitter are included in this model. By comparing with the Matlab model, the SystemC/SystemC-AMS model can dramatically reduce simulation time. Also, by comparing with Analog Devices ADI SimPLL simulation results, Cadence simulation results and real measurement results, the accuracy of the SystemC/SystemC-AMS model is demonstrated. The paper shows the feasibility of a unified design environment for mixed-signal modelling based on SystemC/SystemC-AMS in order to reduce the cost and design time of electrical systems.
Źródło:
International Journal of Electronics and Telecommunications; 2012, 58, 3; 225-232
2300-1933
Pojawia się w:
International Journal of Electronics and Telecommunications
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Argon Plasma in a New Process for Improving the Physical and Anti-bacterial Properties of Crosslinked Cotton Cellulose with Dimethyloldihydroxyethyleneurea-Maleic Acid
Zastosowanie plazmy niskotemperaturowej w środowisku argonu w procesie obróbki tkanin bawełnianych sieciowanych za pomocą kwasu dimetylodihydroxyetylenourylomaleinowego dla polepszenia właściwosci fizycznych oraz antybakteryjnych
Autorzy:
Tsou, C H
Suen, M C
Tsou, C Y
Chen, J C
Yeh, J T
Lin, S M
Lai, Y C
Hwang, J Z
Huang, S H
Hung, W S
Hu, C C
Lee, K R
Lai, J Y
Powiązania:
https://bibliotekanauki.pl/articles/232331.pdf
Data publikacji:
2015
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Biopolimerów i Włókien Chemicznych
Tematy:
cotton
plasma
antibacterial
crosslinking
crosslink length
crosslinks number per anhydroglucose
surface distribution
odour absorption
bawełna
plazma
właściwości antybakteryjne
sieciowanie tkanin bawełnianych
kwas dimetylodihydroxyetylenourylomaleinowy
odporności na zagniecenia
pochłanianie zapachów
żywica termoutwardzalna
Opis:
This study reports the findings of an argon (Ar) plasma treatment added to the traditional pad-dry-cure process between dry and cure treatments. This new process is called the "paddry-plasma-cure process". The crosslinking agent was a mixture of dimethyloldihydroxyethyleneurea (DMDHEU) and maleic acid (MA). Results show that Ar plasma treatment can increase the bonded crosslinking agent (i.e., the nitrogen content). The dry crease recovery angle (DCRA), wet crease recovery angle (WCRA), and tensile strength retention (TSR) of the pad-dry-plasma-cure finished fabrics were higher than those of traditional pad-dry-cure finished fabrics at a given nitrogen content. Additionally it was found that the number of crosslinks per anhydroglucose unit (CL/AGU) and the length of crosslinks of pad-dry-plasma-cure-finished fabrics were higher than that of traditional pad-dry-cure finished fabrics at the same resin concentration in the pad bath. DCRA, WCRA and TSR values of pad-dry-plasma-cure-finished fabrics were higher than those of pad-dry-curefinished fabrics at the same CL/AGU value. However, activation energies for the pad-dryplasma-cure process were higher than those for the pad-dry-cure process. The anti-bacterial ability and odour absorption of the pad-dry-plasma-cure and pad-dry-plasma-cure finished fabrics were higher than those for the pad-dry-cure finished fabrics. The surface distribution of crosslinking agents for the pad-dry-plasma-cure process was higher than that of the pad-dry-cure process. Thus the pad-dry-plasma-cure process is excellent for improving the physical properties, bacterial inhibition, and odour absorption of finished cotton fabrics and for decreasing their formaldehyde release.
Artykułu odnosi się do problematyki możliwości i celowości wprowadzenia modyfikacji plazmowej w warunkach niskociśnieniowych i w środowisku argonu do konwencjonalnego procesu wykończenia przeciw mnącego z zastosowaniem reaktywnych żywic termoutwardzalnych. Badano kąt odprężenia na sucho i na mokro, i stwierdzono polepszenie właściwości odporności na zagniecenia w wyniku dodatkowego zastosowania plazmy. Badano również właściwości antybakteryjne i pochłanianie zapachów, które okazały się korzystniejsze dla dodatkowej obróbki plazmowej. W wyniku zmodyfikowanej obróbki uzyskano mniejsze wydzielanie formaldehydu z gotowej tkaniny.
Źródło:
Fibres & Textiles in Eastern Europe; 2015, 1 (109); 49-56
1230-3666
2300-7354
Pojawia się w:
Fibres & Textiles in Eastern Europe
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ca-Modified Co/SBA-15 Catalysts for Hydrogen Production through Ethanol Steam Reforming
Autorzy:
Chiou, J. Y. Z.
Liu, S. W.
Ho, K. F.
Huang, H. H.
Tang, Ch. W.
Wang, Ch. B.
Powiązania:
https://bibliotekanauki.pl/articles/411682.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
cobalt catalysts
ethanol
steam reforming
Opis:
Hydrogen production through steam reforming of ethanol (SRE) over the Ca-modified Co/SBA-15 catalysts was studied herein to evaluate the catalytic activity, stability andthebehavior of coke deposition.The Ca-modified SBA-15 supports were prepared from the Ca(NO3)2•4H2O (10 wt%) which was incorporated to SBA-15 by incipient wetness impregnation (assigned as Ca/SBA-15) and direct hydrothermal (assigned as Ca-SBA-15) method. The active cobalt species from the Co(NO3)2•6H2O (10 wt%) was loaded to SiO2, SBA-15 and modified-SBA-15 supports with incipient wetness impregnation method to obtain the cobalt catalysts (named as Co/SiO2, Co/SBA-15, Co-Ca/SBA-15 and Co/Ca-SBA-15, respectively). The prepared catalysts were characterized by using X-ray diffraction (XRD), temperature programmed reduction (TPR), transmission electron microscopy (TEM) and BET.The catalytic performance of the SRE reaction was evaluated in a fixed-bed reactor.The results indicated that the Co/Ca-SBA-15 catalystwas preferential among these catalysts and the ethanol can be converted completely at 375 °C. The hydrogen yield (YH2) approached 4.76 at 500 °C and less coke deposited. Further, the long-term stabilitytest of this catalyst approached100h at 500 °C and did not deactivate.
Źródło:
International Letters of Chemistry, Physics and Astronomy; 2014, 5; 1-16
2299-3843
Pojawia się w:
International Letters of Chemistry, Physics and Astronomy
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes in nutrient and heavy metal content levels of sawdust due to vermicomposting by Allomyrina dichotoma subsp. tunobosonis
Autorzy:
Hoang, C.N.
Yang, Z.-W.
Huang, M.-Y.
Lin, K.-H.
Yang, C.-M.
Nguyen, M.K.
Powiązania:
https://bibliotekanauki.pl/articles/81229.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
vermicomposting
organic waste
nutrient
heavy metal content
sawdust substrate
Allomyrina dichotoma
beetle
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2019, 100, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Construction of a recombinant baculovirus expressing swine hepatitis E Virus ORF2 and preliminary research on its immune effect
Autorzy:
Yang, Z.
Wang, K.
Hu, Y.
Yuan, P.
Yang, Y.
Xie, L.Y.
Huang, S.L.
Liu, J.
Ran, L.
Song, Z.H.
Powiązania:
https://bibliotekanauki.pl/articles/2087759.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
swine HEV
ORF2
baculovirus
immunogenicity
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 1; 47-54
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Design, preparation and properties of novel flame retardant thermosetting vinyl ester copolymers based on castor oil and industrial dipentene
Autorzy:
Mao, W.
Li, S.
Li, M.
Huang, K.
Xia, J.
Powiązania:
https://bibliotekanauki.pl/articles/777956.pdf
Data publikacji:
2017
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
castor oil
vinyl ester resin
copolymers
flammability
thermal properties
Opis:
A novel bio-based flame-retardant thermosetting vinyl ester resin monomer was synthesized from castor oil. The chemical structures of the monomer was characterized by FTIR and 1H-NMR. In order to improve its rigidity and expand its application in the field of bio-based materials, it was mixed with certain proportions of another reactive bio-based VER monomer, which had rigid and strong polar groups, and then a series of copolymers were prepared with thermal curing method. Then their tensile property, hardness, morphology of fractured surface, flame retardant property, DMA and thermostability were all investigated. The results indicated that the copolymers had relatively high tensile strength of 11.2 MPa, and the limiting oxygen index is above 23% in all prepared copolymers. DMA demonstrates that the glass transition temperature of the cured resins is up to 56.1°C. Thermogravimetric analysis shows that the copolymers have excellent thermal stability.
Źródło:
Polish Journal of Chemical Technology; 2017, 19, 3; 1-8
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of experimental infections of various Tembusu virus strains isolated from geese, ducks and chickens on ducklings
Autorzy:
Xu, T.
Huang, X.
Li, Y.
Liu, Q.
Liu, X.
Han, K.
Liu, Y.
Yang, J.
Zhao, D.
Bi, K.
Sun, W.
Powiązania:
https://bibliotekanauki.pl/articles/2087719.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
goose-derived
duck-derived
chicken-derived
Tembusu virus
pathogenicity
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 2; 389-396
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of gas holdup on the efficiency of cyclonic-static microbubble flotation column for oily wastewater treatment
Autorzy:
Ning, K.
Huang, G.
Xu, H.
Wu, L.
Li, X.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/208139.pdf
Data publikacji:
2018
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
bubble columns
efficiency
flotation
wastewater treatment
oil water separation
kolumna flotacyjna
efektywność
flotacja
oczyszczanie ścieków
separacja wody
Opis:
A cyclonic-static microbubble flotation column of a novel construction was used in oil-water separation fields and has high efficiency for oil-water separation. The gas holdup is a key parameter for the evaluation of the performance of a flotation column. The gas holdup, closely related to the bubble size, bubble velocity and superficial gas velocity, is one of the most important parameters characterizing the hydrodynamics of a bubble column. The effect of gas holdup in a cyclonic-static microbubble flotation column was investigated. In addition, several operating parameters such as the circulating pressure, superficial gas velocity, and frother consumption were also investigated. The gas holdup was positively correlated to the superficial gas velocity. The gas holdup of clean water and oil wastewater increased along with the increase of the frother consumption. The separation mechanism of cyclonic-static microbubble flotation column was analyzed.
Źródło:
Environment Protection Engineering; 2018, 44, 2; 5-17
0324-8828
Pojawia się w:
Environment Protection Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of Vacuum Hot-Press Process on The Sintered Characteristics and Mechanical Properties of A High-Density Cr-31.2 Mass% Ti Alloy
Autorzy:
Chang, S.-H.
Li, C.-L.
Huang, K.-T.
Yang, T.-H.
Powiązania:
https://bibliotekanauki.pl/articles/356911.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Cr-31.2 mass% Ti alloy
vacuum hot-press sintering
relative density
TRS
electrical conductivity
Opis:
In this study, two different compositions of submicron-structured titanium (760 nm) and micron-structured chromium (4.66 μm) powders were mixed to fabricate Cr-31.2 mass% Ti alloys by vacuum hot-press sintering. The research imposed various hot-press sintering pressures (20, 35 and 50 MPa), while the sintering temperature maintained at 1250°C for 1h. The experimental results showed that the optimum parameters of the hot-press sintered Cr-31.2 mass% Ti alloys were 1250°C at 50 MPa for 1h. Also, the relative density reached 99.94%, the closed porosity decreased to 0.04% and the hardness and transverse rupture strength (TRS) values increased to 81.90 HRA and 448.53 MPa, respectively. Moreover, the electrical conductivity is enhanced to 1.58 × 104 S·cm-1 However, the grain growth generated during the high-temperature and high-pressure of the hot-press sintering process resulted in the grain coarsening phenomenon of the Cr-31.2 mass% Ti alloys after 1250°C hot-press sintering at 50 MPa for 1h. In addition, the Cr-31.2 mass% Ti alloys were fabricated with the submicron-structured titanium (760 nm) and chromium (588 nm) powders showed more effective compaction than the micron-structured titanium (760 nm) and chromium (4.66 μm) powders did. The closed porosity decreases to 0.02% and the hardness values increase to 83.23 HRA. However, the agglomeration phenomenon of the Cr phase and brittleness of the TiCr2 Laves phases easily led to a slight decrease in TRS (400.54 MPa).
Źródło:
Archives of Metallurgy and Materials; 2018, 63, 4; 1715-1723
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of preparation methods of mixed calcium and zinc thermal stabilizers derived from dimer fatty acid and tung-oil based C22 triacid on properties of PVC
Autorzy:
Li, M.
Wang, M.
Li, S.
Huang, K.
Mao, W.
Xia, J.
Powiązania:
https://bibliotekanauki.pl/articles/778123.pdf
Data publikacji:
2017
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
poly(vinyl chloride)
mixed calcium and zinc thermal stabilizer
synthesis technology
thermal stabilization
Opis:
Calcium and zinc salts of dimer fatty acids (DFA-Ca and DFA-Zn) were synthesized using direct neutralization and metathesis technologies, respectively. The adduct of maleic anhydride and methyl eleostearate (MAME) was also converted to the corresponding zinc soap (C22TA-Zn) and calcium soap (C22TA-Ca) by the two different synthetic routes. Mixed Ca/Zn salts between DFA-Ca and DFA-Zn, and between C22TA-Zn and C22TA-Ca were used as thermal stabilizers for poly(vinyl chloride) (PVC). The PVC thermal stability was determined using Congo red test, discoloration test, torque rheological analysis and TGA. Dynamic mechanical properties were also tested. Results indicated that the DFA-Ca/DFA-Zn thermal stabilizer from direct neutralization technology was found to be superior to that of the metathesis product. The C22TA-Ca/C22TA-Zn thermal stabilizer from direct neutralization method had overall superior thermal stability, and displayed modulus and glass transition comparable to that of metathesis product. Direct neutralization method was more excellent and convenient than metathesis technology.
Źródło:
Polish Journal of Chemical Technology; 2017, 19, 2; 78-87
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Event-related desynchronization/synchronization-based volitional cursor control in a two-dimensional center-out paradigm
Autorzy:
Huang, D.
Qian, K.
Oxenham, S.
Fei, D. -Y.
Bai, O.
Powiązania:
https://bibliotekanauki.pl/articles/1396737.pdf
Data publikacji:
2012
Wydawca:
Społeczna Akademia Nauk w Łodzi. Polskie Towarzystwo Sieci Neuronowych
Tematy:
brain-computer interface
BCI
two-dimensional
two-dimensional BCI control
event-related synchronization
event-related desynchronization
EEG
Opis:
To achieve a reliable two-dimensional control by noninvasive EEG-based brain-computer interface (BCI), users are typically required to receive long-term training to learn effective regulation of their brain rhythmic activities, and to maintain sustained attention during the operation. We proposed a two-dimensional BCI using event-related desynchronization and event-related synchronization associated with human natural behavior so that users need neither long-term training nor high mental loads to maintain concentration. In this study, we intended to further investigate the performance of the proposed BCI associated with either physical movement or motor imagery with an online two-dimensional centerout cursor control paradigm. Model adaptation method was employed for better decoding of human movement intention from EEG activities. The results demonstrated an effective center-out cursor control: as high as 77.1% during online control with physical movement and 57.3% with motor imagery. It suggests that two-dimensional BCI control can be achieved without long-term training.
Źródło:
Journal of Artificial Intelligence and Soft Computing Research; 2012, 2, 2; 97-108
2083-2567
2449-6499
Pojawia się w:
Journal of Artificial Intelligence and Soft Computing Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Fabrication of polymer microcomponents using CO2 laser melting technique
Wytwarzanie mikroelementów polimerowych z zastosowaniem techniki topienia laserem CO2
Autorzy:
Tan, W. S.
Zhou, J. Z.
Huang, S.
Zhu, W. L.
Meng, X. K.
Powiązania:
https://bibliotekanauki.pl/articles/947347.pdf
Data publikacji:
2015
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Chemii Przemysłowej
Tematy:
molding
polymer microcomponents
CO2 laser
irradiation
numerical simulation
formowanie
mikroelementy polimerowe
laser CO2
napromienianie
symulacja numeryczna
Opis:
A new method of laser melting molding to produce high-efficiency and high-quality polymer microcomponents was proposed. Numerical simulation was used to analyze the temperature changes of polymer melt during the laser irradiation process. An orthogonal experiment was also employed to investigate the factors influencing molding accuracy. The molding experiments were conducted on molds with various degrees of roughness, and the surface quality of the molded pieces was tested. The simulation analysis and experimental results showed that the laser power plays a critical role in improving the repetition accuracy. The next factors are irradiation time and mold temperature, followed by molding force. Optimized technological parameters (1.2 W of laser power, 6 mm of laser beam width, 6 s irradiation time, 150 N molding force, and 80 °C mold temperature) were applied to obtain a molded pieces with high repetition accuracy and a microstructure dimensional deviation of less than 1 µm. Using a mold with lower surface roughness provides that we can obtain a molded piece with lower roughness, the roughness difference between the mold and the molded piece was less than 0.012 µm.
Zaproponowano nową metodę formowania mikroelementów polimerowych z zastosowaniem topienia laserowego, zapewniającą wysoką wydajność oraz dobrą jakość wytwarzanych mikroelementów. Istotnym parametrem procesu, wpływającym na jakość formowanych elementów, jest temperatura stopu polimeru. Do analizy zmian tej temperatury pod wpływem napromieniania laserem zastosowano symulację numeryczną. Zastosowano także ortogonalny plan eksperymentu w celu zbadania czynników wpływających na dokładność formowania. Formowania przeprowadzono z użyciem form o różnych stopniach chropowatości i zbadano jakość powierzchni mikroelementów. Analiza wyników symulacji i badań eksperymentalnych wykazała, że moc lasera odgrywa kluczową rolę w uzyskaniu powtarzalnej dokładności. Kolejnymi czynnikami są czas napromieniowania i temperatura formy oraz siły formujące. Zoptymalizowane parametry technologiczne (moc lasera 1,2 W, szerokość wiązki lasera 6mm, czas napromieniania 6 s, siła formująca 150 N i temperatura formowania 80 °C) zastosowano do formowania elementów z powtarzalną dużą dokładnością odtwarzania wymiarów (odchylenia wymiarów nie przekraczały 1 µm). Użycie form o mniejszej chropowatości powierzchni pozwalało także uzyskać mniejszą chropowatość mikroelementów, przy czym różnica chropowatości formy i otrzymanego elementu była mniejsza niż 0,012 µm.
Źródło:
Polimery; 2015, 60, 3; 192-198
0032-2725
Pojawia się w:
Polimery
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Generation and characterization of a monoclonal antibody against duck Tembusu virus envelope protein
Autorzy:
Han, K.
Zhao, D.
Liu, Y.
Liu, Q.
Huang, X.
Yang, J.
Bi, K.
Xu, T.
Li, Y.
Powiązania:
https://bibliotekanauki.pl/articles/31495.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused massive economic losses to the duck industry in China. Envelope (E) protein of DTMUV is an important structural protein, which is able to induce protective immune response in target animals and can be used as specific serological diagnosis tool. In this study, a novel monoclonal antibody, designated mAb 3E9, was generated against DTMUV E protein. It is positive in indirect ELISA against both His-E protein and the purified whole viral antigen. Also, this mAb showed positive reaction with DTMUV in Western blot and indirect immunofluorescence assay, and the isotype was IgG1. End-point neutralizing assay performed in BHK-21 cells revealed that the neutralization titer of 3E9 against DTMUV JS804 strain reached 1:50. Furthermore, functional studies revealed that 3E9 blocks infection of DTMUV at a step on viral attachment. The anti-E mAbs produced in the present work may be valuable in developing an antigen-capture ELISA test for antigen detection or a competitive ELISA test for antibody detection or therapeutic medicine for DTMUV in poultry.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genotyping and pathogenic characterization of canine distemper virus based on mutations in the hemagglutinin gene in Chinese domestic dogs
Autorzy:
Chen, M.
Xin, T.
Hou, S.
Lin, W.
Song, W.
Zhu, H.
Huang, K.
Jia, H.
Powiązania:
https://bibliotekanauki.pl/articles/2087680.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
canine distemper virus
hemagglutinin
phylogenetic analysis
animal infection
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 3; 623-629
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
Autorzy:
Dai, G.
Huang, X.
Liu, Q.
Li, Y.
Zhang, L.
Han, K.
Yang, J.
Liu, Y.
Xue, F.
Zhao, D.
Powiązania:
https://bibliotekanauki.pl/articles/16647377.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
epitope
goose astrovirus
capsid protein
monoclonal antibody
Opis:
Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies