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    Wyświetlanie 1-9 z 9
    Tytuł:
    Application of in vitro culture techniques to barley [Hordeum vulgare L.] improvement
    Autorzy:
    Ullrich, S E
    Kleinhofs, A.
    Hou, L.
    Jones, B.L.
    Powiązania:
    https://bibliotekanauki.pl/articles/2044453.pdf
    Data publikacji:
    1998
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    tissue culture
    doubled haploid
    mutagenesis
    breeding programme
    anther culture
    in vitro
    callus culture
    somaclonal variation
    barley
    mutation
    Hordeum vulgare
    embryo
    molecular biology
    Opis:
    Several aspects of in vitro culture have potential for cereal improvement. This paper focuses on evaluation of somaclonal variation (SV) from immature embryo callus culture, and doubled haploid (DH) production via anther culture in barley. Genetically stable SV was observed for several seedling morphological traits such as albino, yellow, light green and lethal. SV occurred at approximately half the frequency of azide-induced mutagenesis. The potential for widespread application of anther culture-mediated DH production in barley breeding and genetic studies was increased through culture procedure improvements and understanding the inheritance of anther culture response. Methodology improvements included substitution of inexpensive gelrite for expensive ficoll or agarose, ability to grow anther donor plants under field as well as growth chamber conditions and flexibility in cold pretreatment/storage of anther donor spikes for 4-6 weeks prior to anther plating. From diallel analysis, inheritance of anther culture response was complex with additive and dominance effects for embryoid formation, total plant regeneration and green plant regeneration and reciprocal effects (maternal) for green plant regeneration. High x low responder crosses generated F₁’s that were intermediate in response and low x low crosses sometimes produced F₁ heterosis for green plant regeneration. Therefore, some recalcitrant types appear to be usable in anther culture DH production systems within a breeding program.
    Źródło:
    Journal of Applied Genetics; 1998, 39, 1; 49-58
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Expression and characterisation of cucumber fruit flesh thickness-related gene CSA2M058670.1
    Autorzy:
    Wang, W.
    Wang, S.
    Guo, Y.
    Li, M.
    Hou, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/12709293.pdf
    Data publikacji:
    2020
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Opis:
    Bulked segregant analysis combined with specific length amplified fragment sequencing techniques have been applied to determine the fine genetic mapping of fruit flesh thickness-related genes in cucumber. Herein, the Csa2M058670.1 gene was subjected to real time fluorescence quantitative PCR (qRT-PCR) and sequence analysis, indicating a strong correlation with cucumber fruit flesh thickness. Expression and characterisation of the Csa2M058670.1 gene were performed based on previous studies. The results of the fluorescence-based quantitative PCR showed that Csa2M058670.1 was expressed in all organs, but levels were highest in fruit peel, fruit flesh, and female flowers. Furthermore, Csa2M058670.1 expression was induced by abiotic stresses including drought, low temperature, and high salt. Domain analysis revealed that the protein encoded by Csa2M058670.1 possesses an SET (Su(var), Enhancern of zeste CE(z), and Trithorax) domain that may control cell division and differentiation. Therefore, we speculated that Csa2M058670.1 might affect fruit flesh thickness in cucumbers by influencing cell division.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2020, 19, 2; 25-33
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Hydrogen sulfide is involved in the chilling stress response in Vitis vinifera L.
    Autorzy:
    Fu, P.
    Wang, W.
    Hou, L.
    Liu, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/56519.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    hydrogen sulphide
    chilling stress
    stress response
    Vitis vinifera
    Opis:
    Hydrogen sulfide (H2S) is an important signaling molecule involved in several stress-resistance processes in plants, such as drought and heavy metal stresses. However, little is known about the roles of H2S in responses to chilling stress. In this paper, we demonstrated that chilling stress enhance the H2S levels, the H2S synthetase (L-/D-cysteine desulfhydrase, L/DCD) activities, and the expression of L/DCD gene in Vitis vinifera L. ‘F-242’. Furthermore, the seedlings were treated with sodium hydrosulfide (NaHS, a H2S donor) and hypotaurine (HT, a H2S scavenger) at 4°C to examine the effects of exogenous H2S on grape. The results revealed that the high activity of superoxide dismutase and enhanced expression of VvICE1 and VvCBF3 genes, but low level of superoxide anion radical, malondialdehyde content and cell membrane permeability were detected after addition of NaHS. In contrast, HT treatment displayed contrary effect under the chilling temperature. Taken together, these data suggested that H2S might be directly involved in the cold signal transduction pathway of grape.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2013, 82, 4
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influence of heat stress on leaf morphology and nitrogen–carbohydrate metabolisms in two wucai (Brassica campestris L.) genotypes
    Autorzy:
    Yuan, L.
    Tang, L.
    Zhu, S.
    Hou, J.
    Chen, G.
    Liu, F.
    Liu, S.
    Wang, C.
    Powiązania:
    https://bibliotekanauki.pl/articles/56883.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Opis:
    Heat stress is a major environmental stress that limits plant growth and yield worldwide. The present study was carried out to explore the physiological mechanism of heat tolerant to provide the theoretical basis for heat-tolerant breeding. The changes of leaf morphology, anatomy, nitrogen assimilation, and carbohydrate metabolism in two wucai genotypes (WS-1, heat tolerant; WS-6, heat sensitive) grown under heat stress (40°C/30°C) for 7 days were investigated. Our results showed that heat stress hampered the plant growth and biomass accumulation in certain extent in WS-1 and WS-6. However, the inhibition extent of WS-1 was significantly smaller than WS-6. Thickness of leaf lamina, upper epidermis, and palisade mesophyll were increased by heat in WS-1, which might be contributed to the higher assimilation of photosynthates. During nitrogen assimilation, WS-1 possessed the higher nitrogen-related metabolic enzyme activities, including nitrate reductase (NR), glutamine synthetase (GS), glutamate synthase (GOGAT), and glutamate dehydrogenase (GDH), which were reflected by higher photosynthetic nitrogen-use efficiency (PNUE) with respect to WS-6. The total amino acids level had no influence in WS-1, whereas it was reduced in WS-6 by heat. And the proline contents of both wucai genotypes were all increased to respond the heat stress. Additionally, among all treatments, the total soluble sugar content of WS-1 by heat got the highest level, including higher contents of sucrose, fructose, and starch than those of WS-6. Moreover, the metabolism efficiency of sucrose to starch in WS-1 was greater than WS-6 under heat stress, proved by higher activities of sucrose phosphate synthase (SPS), sucrose synthase (SuSy), acid invertase (AI), and amylase. These results demonstrated that leaf anatomical alterations resulted in higher nitrogen and carbon assimilation in heat-tolerant genotype WS-1, which exhibited a greater performance to resist heat stress.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2017, 86, 2
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prevalence of pathogenicity island ETT2 in Escherichia coli isolated from piglets with diarrhea in northeast of China
    Autorzy:
    Yuan, C.W.
    Liu, W.X.
    Hou, J.L.
    Zhang, L.G.
    Wang, G.Q.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087783.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Escherichia coli
    pathogenicity island
    ETT2
    piglets
    diarrhea
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 1; 5-12
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Sexual dimorphism in perissodactyl rhinocerotid Chilotherium wimani from the Late Miocene of the Linxia Basin (Gansu, China)
    Autorzy:
    Chen, S.
    Deng, T.
    Hou, S.
    Shi, Q.
    Pang, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/23414.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Instytut Paleobiologii PAN
    Tematy:
    sexual dimorphism
    perissodactyl rhinocerotid
    Mammalia
    Perissodactyla
    Chilotherium wimani
    Late Miocene
    Miocene
    Linxia basin
    China
    Opis:
    Sexual dimorphism is reviewed and described in adult skulls of Chilotherium wimani from the Linxia Basin. Via the analysis and comparison, several very significant sexually dimorphic features are recognized. Tusks (i2), symphysis and occipital surface are larger in males. Sexual dimorphism in the mandible is significant. The anterior mandibular morphology is more sexually dimorphic than the posterior part. The most clearly dimorphic character is i2 length, and this is consistent with intrasexual competition where males invest large amounts of energy jousting with each other. The molar length, the height and the area of the occipital surface are correlated with body mass, and body mass sexual dimorphism is compared. Society behavior and paleoecology of C. wimani are different from most extinct or extant rhinos. M/F ratio indicates that the mortality of young males is higher than females. According to the suite of dimorphic features of the skull of C. wimani, the tentative sex discriminant functions are set up in order to identify the gender of the skulls.
    Źródło:
    Acta Palaeontologica Polonica; 2010, 55, 4
    0567-7920
    Pojawia się w:
    Acta Palaeontologica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Synchronization and stability of an elastically coupled tri-rotor vibration system
    Autorzy:
    Hou, Y.
    Du, M.
    Fang, P.
    Zhang, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/279489.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Mechaniki Teoretycznej i Stosowanej
    Tematy:
    tri-rotor
    synchronization characteristics
    stability
    computer simulations
    Opis:
    A new mechanism, an elastically coupled tri-rotor system, is proposed to implement synchronization. It is composed of a rigid body, three induction motors, coupling unit and springs. According to the Lagrange equation, the model of the system is established. The average method of small parameters is applied to study the synchronization characteristics of the system, therefore, the balance equation and stability criterion of the system can be obtained. Obviously, many parameters affect the synchronous state of the rotors, especially the spring stiffness, the stiffness of the coupling unit and the installation location of the system. Finally, computer simulations are used to verify the correctness of theoretical analysis.
    Źródło:
    Journal of Theoretical and Applied Mechanics; 2017, 55, 1; 227-240
    1429-2955
    Pojawia się w:
    Journal of Theoretical and Applied Mechanics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Vibration analysis of ball bearing considering waviness under high speed and an axial load
    Autorzy:
    Hou, P. P.
    Wang, L. Q.
    Peng, Q. Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/201434.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    rotational speed
    ball bearing
    vibration characteristics
    frequency band
    RMS
    Opis:
    Based on the rolling bearing vibration measurement principle in ISO standard, a nonlinear dynamic model of ball bearing is built and motion equations of the inner ring, outer ring, and rolling elements are derived by using Lagrange’s equation. The ball bearing model includes the influence of waviness, rotational speed, external load, arbor supporting stiffness and arbor eccentricity. Ball bearing high-speed vibration tests are performed and used to verify the theoretical results. Simulated results showed that specific waviness orders produced the principal frequencies that were proportional to rotational speed. Rotational speed mainly affected the value of the natural frequency of the bearing system, and RMS (Root Mean Square) of the full band had a great fluctuation with the increase of rotational speed. In the experiment, spectrum and RMS of 2 ƒs-30 kHz (ƒs : the rotational frequency of inner ring/arbor) under high speed could include not only the influence of rotational speed but also principal frequencies produced by waviness, which could cover the part of requirements of the standard bearing vibration measurement.
    Źródło:
    Bulletin of the Polish Academy of Sciences. Technical Sciences; 2020, 68, 3; 517-527
    0239-7528
    Pojawia się w:
    Bulletin of the Polish Academy of Sciences. Technical Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    VvWRKY13 enhances ABA biosynthesis in Vitis vinifera
    Autorzy:
    Hao, J.
    Ma, Q.
    Hou, L.
    Zhao, F.
    Liu, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/58583.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Opis:
    Abscisic acid (ABA) plays critical roles in plant growth and development as well as in plants’ responses to abiotic stresses. We previously isolated VvWRKY13, a novel transcription factor, from Vitis vinifera (grapevine), and here we present evidence that VvWRKY13 may regulate ABA biosynthesis in plants. When VvWRKY13 was ectopically expressed in Arabidopsis, the transgenic lines showed delayed seed germination, smaller stomatal aperture size, and several other phenotypic changes, indicating elevated ABA levels in these plants. Sequence analysis of several genes that are involved in grapevine ABA synthetic pathway identified WRKY-specific binding elements (W-box or W-like box) in the promoter regions. Indeed, transient overexpression of VvWRKY13 in grapevine leaves significantly increased the transcript levels of ABA synthetic pathway genes. Taken together, we conclude that VvWRKY13 may promote ABA production by activating genes in the ABA synthetic pathway.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2017, 86, 2
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-9 z 9

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