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Wyświetlanie 1-5 z 5
Tytuł:
Cellular energy allocation in the predatory bug, Andrallus spinidens Fabricius (Hemiptera: Pentatomidae), following sublethal exposure to diazinon, fenitrothion, and chlorpyrifos
Autorzy:
Chitgar, M.G.
Hajizadeh, J.
Ghadamyari, M.
Karimi-Malati, A.
Sharifi, M.
Hoda, H.
Powiązania:
https://bibliotekanauki.pl/articles/66608.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
It is necessary to study the biochemical changes in insects exposed to toxicants if we want to predict the potential of various chemicals on the natural enemy. Physiological energy, as a biochemical biomarker, may be affected by many pesticides including organophosphate compounds. Therefore, in this study, the sublethal effects of diazinon, fenitrothion, and chlorpyrifos on the cellular energy allocation (CEA) of the predatory bug, Andrallus spinidens Fabricius (Hemiptera: Pentatomidae), a potential biological control agent, was studied on 5th-instar nymphs. Among the energy reserves of the A. spinidens nymphs, only total protein was significantly affected by pesticide treatments, and the highest value was observed in chlorpyrifos treatment. The energy available (Ea) and energy consumption (Ec) in A. spinidens were significantly affected by these pesticides. In exposed bugs, these parameters were affected by fenitrothion and chlorpyrifos more than diazinon. The activity of the electron transport system (ETS) in the Ec assay showed that A. spinidens exposed to chlorpyrifos had the highest rate of oxygen consumption. Although, there was no significant change in CEA, the insecticides caused a marked change in the physiological balance of A. spinidens. The results suggested that the adverse effect of these insecticides on A. spinidens should be considered in Integrated Pest Management (IPM) programs.
Źródło:
Journal of Plant Protection Research; 2014, 54, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Purification and characterization of the cuticle-degrading proteases produced by an isolate of Beauveria bassiana using the cuticle of the predatory bug, Andrallus spinidens Fabricius (Hemiptera: Pentatomidae)
Autorzy:
Firouzbakht, H.
Zibaee, A.
Hoda, H.
Sohani, M.M.
Powiązania:
https://bibliotekanauki.pl/articles/65065.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The entomopathogenic fungi-like Beauveria bassiana must penetrate via the integument of an insect to reach the hemocoel. Since proteins are the molecules responsible for integument strength in insects, the proteins must synthesise the cuticle degrading proteases which will then enable the proteases to penetrate. It is important to determine the biochemical properties of these proteases so that fungal virulence can be better understood. In the current study, a recently collected isolate of B. bassiana, namely AM-118, was inoculated in liquid media containing 0.5% of Andrallus spinidens Fabricus cuticle to obtain specific proteases. The crude samples were purified via a three step process using ammonium sulfate, Sepharyl G-100, and DEAE-Cellulose Fast Flow. The results revealed two proteases known as subtilisin-like (Pr1), and trypsin-like (Pr2), with the molecular weights of 105 and 103 kDa. The optimal pH and temperature values were found to be 8 and 35°C for Pr1 and 8 and 40°C for Pr2, respectively. Inhibitors like AEBSF, EDTA, TPCK, and phenanthroline significantly affected proteolytic activities. Here, we reported two fungal proteases by high molecular weight from an Iranian isolate of B. bassiana. These findings will help us to better understand fungal virulence against insects.
Źródło:
Journal of Plant Protection Research; 2015, 55, 2
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Elektroforeza swobodna z przepływem hydrodynamicznym : podstawy teoretyczne i rozwiązania techniczne
Free flow electrophoresis : theory and technology
Autorzy:
Hoda, P.
Polak, B.
Dzido, T. H.
Powiązania:
https://bibliotekanauki.pl/articles/172756.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Chemiczne
Tematy:
elektroforeza swobodna
przepływ hydrodynamiczny
technika elektromigracyjna
rozdzielanie pseudo-dwuwymiarowe
rozdzielanie preparatywne
mikro-elektroforeza swobodna
free flow electrophoresis
electromigration technique
pseudo-two dimensional separation
preparative separation
micro free flow electrophoresis
Opis:
Free flow electrophoresis, or carrier-free electrophoresis, is a technique based upon the principle of mobility of chargeable species in the electric field. However, unlike more widespread gel electrophoresis, free flow electrophoresis does not utilise any kind of solid support medium. Instead, species to be separated move within the space filled with aqueous buffer, which is constantly pumped in the direction perpendicular to the direction of the applied electric field. Such set-up allows for continuous separation, as compounds of the sample being processed form separate bands and leave the separation system through several different outlets located along the edge of the chamber. Furthermore, the use of mild separation conditions increases the chance of biologically active compounds retaining their activity after the separation is finished. These qualities make free flow electrophoresis an excellent tool for protein research and cytology. In this review, the basic theoretical aspects of the technique are outlined, with a special emphasis placed on various modes in which free flow electrophoresis can be operated. Besides, a review of milestone papers related to free flow electrophoresis technology is presented. Apart from the devices following the original concept of Barrollier and Hannig, an insight into the construction of recirculating instrumentation, multicompartment electrolysers as well as radially-symmetric chambers is provided. A special focus is placed on patents and commercialized solutions. Finally, the challenges of scaling-down free flow electrophoresis to micro-dimensions are introduced.
Źródło:
Wiadomości Chemiczne; 2018, 72, 1-2; 39-60
0043-5104
2300-0295
Pojawia się w:
Wiadomości Chemiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibiotic resistance and siderophores production by clinical Escherichia coli strains
Autorzy:
Khazaal, Mohamed T.
El-Hendawy, Hoda H.
Mabrouk, Mona I.
Faraag, Ahmed H.I.
Bakkar, Marwa R.
Powiązania:
https://bibliotekanauki.pl/articles/16648088.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
siderophores
Escherichia coli
clinical strains
antibiotic susceptibility
Opis:
The phenomenon of antibiotic resistance has dramatically increased in the last few decades, especially in enterobacterial pathogens. Different strains of Escherichia coli have been reported to produce a variety of structurally different siderophores. In the present study, 32 E. coli strains were collected from different clinical settings in Cairo, Egypt and subjected to the antibiotic susceptibility test by using 19 antibiotics belonging to 7 classes of chemical groups. The results indicated that 31 strains could be considered as extensively drug-resistant and only one strain as pan drug-resistant. Siderophores production by all the tested E. coli strains was determined qualitatively and quantitatively. Two E. coli strains coded 21 and 49 were found to be the most potent siderophores producers, with 79.9 and 46.62%, respectively. Bacterial colonies with cured plasmids derived from strain 49 showed susceptibility to all the tested antibiotics. Furthermore, E. coli DH5α cells transformed with the plasmid isolated from E. coli strain 21 or E. coli strain 49 were found to be susceptible to ansamycins, quinolones, and sulfonamide groups of antibiotics. In contrast, both plasmid-cured and plasmid-transformed strains did not produce siderophores, indicating that the genes responsible for siderophores production were located on plasmids and regulated by genes located on the chromosome. On the basis of the obtained results, it could be concluded that there is a positive correlation between antibiotic resistance, especially to quinolones and sulfonamide groups, and siderophores production by E. coli strains used in this study.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2022, 103, 2; 169-184
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes in cellular immune responses of Chilo suppressalis Walker (Lepidoptera: Crambidae) due to pyriproxyfen treatment
Autorzy:
Mirhaghparast, S.K.
Zibaee, A.
Hoda, H.
Sendi, J.J.
Powiązania:
https://bibliotekanauki.pl/articles/66690.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cellular immune response
Chilo suppressalis
Asiatic rice borer
striped rice stemborer zob.Asiatic rice borer
Lepidoptera
Crambidae
pyriproxyfen
treatment
Beauveria bassiana
Opis:
The effects of pyriproxyfen were determined on the cellular immunity and phenoloxidase activity in the 4th instar larvae of Chilo suppressalis Walker. The bioassay results revealed the effective concentrations of: 10L : 18C, 30L : 72C and 50L : 190C μg · ml–1. The sole effect of 18 and 72 μg · ml–1 concentrations at intervals of 1–3 h caused a higher number of total hemocytes in the treated larvae than the control, but the reverse results were observed after 6–24 h. The number of plasmatocytes was lower than that of the control for intervals of 3–24 h but the number of granulocytes was higher than the control after 1–3 h although no significant differences were observed at the other times. In the treated larvae, the activities of phenoloxidase were higher and lower than those of the control after 1–3 h and 6–24 h, respectively. The combined effects of pyriproxyfen and the entomopathogenic fungus, Beauveria bassiana isolate B3 caused higher numbers of total hemocytes, plasmatocytes, and granulocytes in the treated larvae by use of the three concentrations of pyriproxyfen, at intervals of 6 and 12 h. Although the numbers of nodules in the larvae treated with concentrations of 18 μg · ml–1 were higher than those of other treatments, the overall numbers were lower than those of the control. Finally, the activity of phenoloxidase in the treated larvae was higher than that of the control, at intervals of 6 and 12 h post-treatment. Findings of the current study indicate an intervening role of pyriproxyfen in the cellular immunity of C. suppressalis to entomopathogenic objects.
Źródło:
Journal of Plant Protection Research; 2015, 55, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-5 z 5

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