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    Tytuł:
    Effect of PEG 4000 on maturation, germination and conversion frequency of Picea abies and P. omorika somatic embryos
    Autorzy:
    Wawrzyniak, M.
    Hazubska-Przybyl, T.
    Obarska, A.
    Bojarczuk, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/951243.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    polyethylene glycol
    somatic embryogenesis
    somatic seedling
    maturation
    germination
    coniferous species
    Picea abies
    Picea omorika
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro cultures of Populus × canescens inoculated with various strains of ectomycorrhizal Paxillus involutus fungi under lead ion stress
    Autorzy:
    Szuba, A.
    Karlinski, L.
    Hazubska-Przybyl, T.
    Mucha, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/951315.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    ectomycorrhizal fungi
    in vitro culture
    Paxillus involutus
    Populus × canescens
    lead ion
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Somatic embryogenesis of selected coniferous tree species of the genera Picea, Abies and Larix
    Autorzy:
    Szczygiel, K
    Hazubska-Przybyl, T.
    Bojarczuk, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/57833.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    Abies
    micropropagation
    ex vitro
    development
    larch
    tree species
    spruce
    in vitro
    botany
    Picea
    medium
    embryogenesis
    Larix
    fir
    hormone
    Opis:
    Experiments on somatic embryogenesis in selected spruce, fir and larch species were performed to determine if this method of micropropagation enables production of quality seedlings for forest nurseries. High frequencies of embryogenic callus in spruce (23-31%) and fir (29%) were achieved when mature zygotic embryos were used as explants, while in larches (36%) only when megagametophytes (endosperms with immature embryos) were used. The possibility of somatic embryogenesis initiation also from somatic embryos (resulting in secondary and third generation of embryogenic callus) indicate high efficiency of this method of micropropagation. The best results at all stages of somatic embryogenesis (good proliferation of embryogenic callus, high rate of embryo regeneration and their survival) were obtained for Norway spruce, European larch and some hybrid larches.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2007, 76, 1
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Growth regulators and guaiacol peroxidase activity during the induction phase of somatic embryogenesis in Picea species
    Autorzy:
    Hazubska-Przybyl, T.
    Ratajczak, E.
    Kalemba, E.M.
    Bojarczuk, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/41387.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Opis:
    Biochemical studies during the induction phase of somatic embryogenesis in Picea abies [L.] Karst. and P. omorika [Pančić] Purk. can supplement our basic knowledge of the developmental processes accompanying the formation of embryogenic tissues from explants. Such studies may also contribute to finding the markers specific to the early stages of somatic embryogenesis of spruce species and, consequently, to the optimization of the process of initiation of embryogenic tissues from different types of plant explants treated with various growth regulator combinations. In this paper the effect of certain growth regulator systems on enzymatic activity was studied. The analysis of guaiacol peroxidase activity (EC 1.11.1.7), based on the spectrophotometric method, showed that this activity was the lowest in mature zygotic embryos (explants) and significantly higher in 8-week-old embryogenic and non-embryogenic calluses treated with various combinations of growth regulators. In the newly initiated embryogenic tissue, the activity of this enzyme decreased and remained at a lower level during proliferation, irrespective of the applied growth regulator combination. The type and concentration of growth regulators used for the initiation and proliferation of embryogenic tissues had no statistically significant effect on peroxidase activity, although during the initiation often its increased level was observed in calluses treated with 2.4-D. Detection of guaiacol peroxidase activity in the induction phase of somatic embryogenesis proves its participation in this process. The subsequent change in its activity indicates that this peroxidase can be a biochemical marker of somatic embryogenesis of the tested spruce species.
    Źródło:
    Dendrobiology; 2013, 69
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro responses of various explants of Fagus sylvatica
    Autorzy:
    Hazubska-Przybyl, T.
    Chmielarz, P.
    Bojarczuk, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/41768.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    European beech
    plant response
    explant
    Fagus sylvatica
    micropropagation
    culture condition
    tissue culture
    in vitro culture
    Opis:
    There are limited published data on in vitro reproduction of Fagus sylvatica L. (European beech). This study was aimed to determine the efficiency of induction of somatic embryogenesis or organogenesis of beech from different types of explants in various culture conditions. Explants derived from immature, fresh seeds (collected in 2011 and 2013) and from mature seeds, stored at –10ºC and some stratified at 3ºC, were placed on induction media with various combinations of plant growth regulators: zeatin, 2,4-dichlorophenoxyacetic acid (2,4-D) and/or benzyladenine (BA). Initial cultures were kept in darkness or weak light (white fluorescent or blue-red LED). Limited success has been achieved in initiation of somatic embryogenesis. We obtained friable, yellow-white callus with characteristic PEM-like structures (cPEM-ls, from embryonic axes or fragments of immature embryos with embryonic axes), which may be an early developmental stage of embryogenic callus of Fagus sylvatica. This type of callus regenerated from explants incubated in darkness, mainly on WPM medium with addition of 6.8 μM zeatin or WPM and MSG media with 9.1 μM 2,4-D and 2.2 μM BA. The highest frequency of regeneration of callus with cPEM-ls was 5%. Instead, we succeeded to induce organogenesis from both immature and mature zygotic embryos and from embryonic axes. The best results were obtained for mature zygotic embryos incubated on ½WPM medium (half-strength Woody Plant Medium) with 9.1 μM 2,4-D and 2.2 μM BA. Adventitious buds were regenerated on up to 15% of the explants. The induced buds developed into shoots, enabling us to establish tissue cultures of beech. Induction of organogenesis from the tested explants was more efficient than induction of somatic embryogenesis.
    Źródło:
    Dendrobiology; 2015, 73
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Potential possibilities of the induction of European beech (Fagus sylvatica ) tissue cultures from various types of explants
    Autorzy:
    Hazubska-Przybyl, T.
    Chmielarz, P.
    Bojarczuk, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/951305.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Fagus sylvatica
    European beech
    tissue culture
    reproduction
    vegetative propagation
    somatic embryogenesis
    organogenesis
    2,4-dichlorophenoxyacetic acid
    benzyladenine
    zeatin
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Somatic embryogenesis of selected spruce species [Picea abies, P. omorika, P. pungens 'Glauca' and P. breweriana]
    Autorzy:
    Hazubska-Przybyl, T
    Bojarczuk, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/56682.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    somatic embryogenesis
    spruce species
    Picea abies
    Picea omorika
    Picea pungens
    Picea breweriana
    plant tissue
    growth regulator
    somatic embryo
    nursery production
    Opis:
    Somatic embryogenesis was studied in four spruce species (Picea abies, P. omorika, P. pungens 'Glauca' and P. brewenana) to determine if this method can be used for in vitro propagation of coniferous trees. The highest frequency of initiation of embryogenic tissue was obtained when mature zygotic embryos were used as explants. It ranged then from 10.8% (P. brewenana) to 23.75% (P. omorika and P. pungens 'Glauca'). The frequency of embryogenic tissue initiation was strongly affected by medium composition, i.e. addition of appropriate auxins (2,4-D, NAA, Picloram) and sucrose concentration (10-20 g-1"1). A lower frequency was obtained in Picea omorika (10%) when megagametophytes (endosperms with immature zygotic embryos) were used as explants. No emryogenic tissue was produced from hypocotyls, cotyledons and needles. A satisfactory frequency was achieved with the use of somatic embryos of Picea abies (30%). The proliferation of embryogenic cell lines of spruces was affected by medium type. The experiments resulted in production of somatic plantlets of P. abies and P. omorika. This enables the application of this method of spruce micropropagation for genetic and breeding research or for nursery production.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2008, 77, 3; 189-199
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Structure of embryogenic tissues and accumulation of storage materials in somatic embryos of Picea abies and P. omorika
    Autorzy:
    Hazubska-Przybyl, T
    Bojarczuk, K.
    Guzicka, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/41109.pdf
    Data publikacji:
    2008
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    Picea abies
    Picea omorica
    tissue
    spruce
    development
    starch grain
    lipid body
    embryogenic tissue
    storage material
    somatic embryo
    plant tissue
    Opis:
    Microscopic analysis of embryogenic tissues of Picea abies and P. omorika showed that the tissues differed in proportions of various types of proembryogenic masses, and in presence/absence of early somatic embryos, which suggests that they differed in embryogenic potential. On the basis of this study, embryogenic competence of tissues can be assessed at early stages of somatic embryogenesis, and thus the most efficient lines can be selected (one Picea abies and two P. omorika). In the course of development of somatic embryos embryogenic tissue was placed on a medium with abscisic acid (ABA), which affects an increase in osmotic pressure at the stage of maturation of somatic embryos and finally on the regeneration of cotyledonary embryos. In this stage of embryos development, storage materials were accumulated, which are used in specific metabolic processes during embryo development.
    Źródło:
    Dendrobiology; 2008, 60; 19-28
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Tree somatic embryogenesis in science and forestry
    Autorzy:
    Hazubska-Przybyl, T.
    Bojarczuk, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/41255.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    tree
    somatic embryogenesis
    embryogenesis
    science
    forestry
    in vitro culture
    seedling
    somatic seedling
    forest management
    Opis:
    Somatic embryogenesis is the latest, and potentially the most efficient, method for the vegetative micropropagation of plants. Over the past three decades, numerous laboratory studies have investigated somatic embryogenesis of forest trees, yielding positive results for a number of economically important tree species. The first test trials were run and plantations were planted with interior spruce in the 90s by CellFor Inc. (Canada). However, at the beginning of the XXI century, the program to produce spruce and Douglas fir somatic seedlings was stopped for economic reasons. Thus, currently no operational program is ongoing except on a small scale in New Brunswick. In order to integrate somatic embryogenesis technology into operational reforestation programs, the production costs of forest tree somatic seedlings needs to be reduced, and the awareness of foresters and forest landowners that the material obtained through somatic embryogenesis is valuable needs to be increased. This awareness would enable implementation of this technology on a large scale for production and forest management throughout Europe including Poland. In this review, the importance of somatic embryogenesis in scientific research and in global and European forestry is presented. Our main aims are to provide basic information on the challenges in researching somatic embryogenesis of forest trees and to raise interest in this tree propagation technique in both scientists and foresters.
    Źródło:
    Dendrobiology; 2016, 76
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Induction of somatic embryogenesis in spruce: Picea omorika, P. pungens "Glauca", P. breweriana and P. abies
    Autorzy:
    Hazubska, T
    Szczygiel, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/41201.pdf
    Data publikacji:
    2003
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    Norway spruce
    Picea pungens
    Serbian spruce
    Picea abies
    blue spruce
    Picea omorika
    clonal propagation
    spruce
    somatic embryogenesis
    maturation
    embryogenesis
    Picea breweriana
    somatic embryo
    Opis:
    The studies were carried out on seed explants (mature zygotic embryos) of four spruce species: Picea omorika (Pancić) Purk., P. pungens ‘Glauca' Beisnn., P. breweriana S. Watson and P. abies (L.) Karst. The explants required darkness during the induction of embryogenic tissue. Temporary exposure of explants to light did not increase their embryogenic capacity. It was observed that the temperature of 25°C was the optimal for embryogenic tissue induction from mature zygotic embryos of Picea omorika. Cold treatment at 4°C during one week improved embryogenic tissue initiation in the explants of P. pungens ‘Glauca'. Seed explants of Picea omorika produced embryogenic tissue on medium lacking glutamine. Only explants of Picea abies and P. omorika showed embryogenic tissue formation. For effective proliferation of embryogenic tissue in Picea abies and P. omorika it was necessary to supply the medium with 2,4-D (4.5-9 μM) and BA (2.25 μM). Two of three analized lines showed that ABA promote somatic embryo maturation, at the ABA concentration 20 μM in the medium.
    Źródło:
    Dendrobiology; 2003, 50
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-10 z 10

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