Autor: Harnisz, Monika - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: The effects of season and processing technology on the abundance of antibiotic resistance genes in air samples from municipal wastewater treatment and waste management plants
Autorzy:: Osińska, Adriana
Jachimowicz, Piotr
Niestępski, Sebastian
Harnisz, Monika
Korzeniewska, Ewa
Powiązania:: https://bibliotekanauki.pl/articles/2032941.pdf
Data publikacji:: 2021
Wydawca:: Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:: municipal wastewater
antibiotic resistance
wastewater treatment plant
rRNA
ścieki komunalne
odporność na antybiotyki
oczyszczalnia ścieków
Opis:: This study aimed to perform a qualitative and a quantitative assessment of the prevalence of genes encoding resistance to beta-lactam, tetracycline, and chloramphenicol antibiotics in samples of DNA isolated from air in a municipal wastewater treatment plant (WWTP) and a municipal waste management plant (WMP). Air samples were collected in the mechanical (MP) and biological (BP) processing units of WWTP and WMP in winter and spring. The samples of air were collected by impingement into PBS solution and subsequently, DNA was isolated. The prevalence of the 16S rRNA gene and ARGs was determined by PCR, and the most abundant ARGs were quantified by qPCR. The highest diversity of the analyzed ARGs was noted in air samples collected in the mechanical processing units of the WWTP (winter) and the WMP (spring). The copy of ARGs varied between treatment units and seasons. ARGs were most abundant in air samples collected in spring in the MP units of both the WWTP and the WMP. The study demonstrated that ARGs are ubiquitous in the air in both WWTPs and WMPs. The presence of ARGs in the air can exert a negative impact on the health of plant employees.
Źródło:: Environment Protection Engineering; 2021, 47, 1; 101-114
0324-8828
Pojawia się w:: Environment Protection Engineering
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: The Prevalence of tet(A) and tet(M) Tetracycline Resistance Genes in Municipal Wastewater
Autorzy:: Hubeny, Jakub
Buta, Martyna
Zieliński, Wiktor
Harnisz, Monika
Korzeniewska, Ewa
Nowrotek, Monika
Płaza, Grażyna
Powiązania:: https://bibliotekanauki.pl/articles/123400.pdf
Data publikacji:: 2019
Wydawca:: Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:: WWTP
tetracyclines
ARGs
ARB
antibiotic resistance
environment
wastewater
Opis:: Antibiotic resistance is a widespread problem that poses one of the greatest risks to public health around the world. The main cause of antibiotic resistance is the overuse of antibiotics in the human and veterinary medicine and in agriculture. Drugs are released into the environment with treated wastewater, and they can act as stressors that increase the prevalence of antibiotic resistance genes (ARGs). Wastewater treatment plants (WWTPs) are not equipped with appropriate technologies for eliminating the genetic material from the treated wastewater. In this study, the prevalence of tet(A) and tet(M) genes encoding resistance to tetracycline antibiotics was investigated in the samples of municipal wastewater and sewage sludge collected from two WWTPs and in the water samples collected from rivers which receive the treated wastewater. The samples were collected in two seasons of the year (summer and fall). The presence of ARGs was confirmed by PCR. The study revealed that ARGs were not effectively removed from wastewater by the WWTP in the Region of Silesia. Seasonal variations in the occurrence of the analyzed genes were not observed in the samples collected from the above-mentioned plant. Tetracycline resistance genes were detected in all samples of river water. The tet(A) gene was not removed from the treated wastewater in the WWTP in the Region of Warmia and Mazury, whereas the tet(M) gene was detected on a seasonal basis. The tet(M) gene was not detected in the samples of river water collected upstream and downstream from the WWTP. The study demonstrated that the existing WWTPs lack the means to eliminate ARGs. The wastewater treatment systems have to be modified to effectively remove ARGs from the treated wastewater.
Źródło:: Journal of Ecological Engineering; 2019, 20, 10; 1-6
2299-8993
Pojawia się w:: Journal of Ecological Engineering
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Prevalence of Beta Lactamases Genes in Sewage and Sludge Treated in Mechanical-Biological Wastewater Treatment Plants
Autorzy:: Zieliński, Wiktor
Buta, Martyna
Hubeny, Jakub
Korzeniewska, Ewa
Harnisz, Monika
Nowrotek, Monika
Płaza, Grażyna
Powiązania:: https://bibliotekanauki.pl/articles/124493.pdf
Data publikacji:: 2019
Wydawca:: Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:: WWTP
beta-lactamases
wastewater treatment
antibiotic resistance genes
Opis:: Wastewater treatment plants (WWTPs) are a very important link in the spread of antibiotic resistance genes to the environment and the formation of antibiotic-resistant microorganisms. The mechanical and biological methods of wastewater treatment in WWTPs do not completely remove the resistance genes from sewage. The genes responsible for extended-spectrum beta-lactamases (ESBLs) are very common in the family Enterobacteriaceae that colonize the human digestive tract and are abundant in wastewater. The aim of this study was to analyze the prevalence of genes encoding beta-lactamases in the wastewater and sludge samples collected from two WWTPs in the Polish regions of Warmia and Silesia and from the river water upstream and downstream from the WWTPs. The wastewater samples were passed through polycarbonate membrane filters, whereas the sludge samples were homogenized, and genomic DNA was extracted. The bla_TEM, bla_OXA and bla_SHV genes were detected by means of standard PCR. The most prevalent gene was bla_TEM which occurred in all samples, including the treated wastewater. The bla_OXA gene was also frequently detected in all samples from the WWTP in Silesia. The bla_SHV gene was least prevalent in the tested samples. These results indicate that wastewater is a hotspot for resistant bacteria. Beta-lactamase genes are not eliminated through the mechanical-biological wastewater treatment methods, and they can spread to other environments, thus increasing the pool of antibiotic resistance genes around the world and creating epidemiological risks.
Źródło:: Journal of Ecological Engineering; 2019, 20, 9; 80-86
2299-8993
Pojawia się w:: Journal of Ecological Engineering
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: The Occurrence of Integrase Genes in Different Stages of Wastewater Treatment
Autorzy:: Buta, Martyna
Hubeny, Jakub
Zieliński, Wiktor
Korzeniewska, Ewa
Harnisz, Monika
Nowrotek, Monika
Płaza, Grażyna
Powiązania:: https://bibliotekanauki.pl/articles/124594.pdf
Data publikacji:: 2019
Wydawca:: Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:: integrase
integron
ARGs
wastewater
Opis:: The uncontrolled use of antibiotics and the release of drug residuals into the environment contribute to antibiotic resistance and constitute a serious public health threat. The spread of antibiotic resistance can be attributed mainly to the huge amounts of bacteria harboring the antibiotic resistance genes (ARGs) which are released into the environment with the treated wastewater. The molecular mechanisms of antibiotic resistance, in which the mobile genetic elements (MGEs) such as plasmids, transposons, bacteriophages and integrons associated with the transfer of ARGs play the main role, should be broadly investigated to develop effective methods for addressing this problem. This study focused mainly on integrons which: (i) are the simple elements involved in the mobility of gene cassettes, (ii) have a common structure, (iii) can be associated with other MGEs, and (iv) are particularly efficient in trapping ARGs. The aim of the study was to estimate the efficiency of different stages of the wastewater treatment process in removing class 1, 2 and 3 integrase genes in two wastewater treatment plants (WWTPs) in Poland and to investigate the presence of these genes in river water upstream and downstream from the effluent discharge point. The presence of intI1, intI2 and intI3 genes was analysed by means of standard PCR with specific primers and a thermal cycling profiles. The samples of wastewater and sludge were collected from two WWTPs located in the Polish regions of (a) Warmia and Mazury, and (b) Silesia. The samples of river water were also collected upstream and downstream from the examined WWTPs. In the selected WWTPs, wastewater is treated through the activated sludge process with various modifications. The presence of intI1, intI2 and intI3 genes in different stages of wastewater treatment was characterized by a similar pattern. The results of this study indicate that WWTPs are not highly effective in removing the integrase genes from all three integron classes. The study revealed somewhat unexpected results, which indicate that the recently discontinued monitoring of the intI3 gene in the wastewater treatment process should be reinstated. The existing wastewater treatment systems should be improved and modified to effectively eliminate the integrase genes from wastewater and prevent contamination of the surface water.
Źródło:: Journal of Ecological Engineering; 2019, 20, 10; 39-45
2299-8993
Pojawia się w:: Journal of Ecological Engineering
Dostawca treści:: Biblioteka Nauki

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