Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

English (EN)·Polski (PL)·Yкраїнський (UK)
Przejdź do strony domowej biblioteki Centralna Biblioteka Wojskowa Link otwiera się w nowym oknie Logo biblioteki Prolib Integro - strona głównaCentralna Biblioteka Wojskowa

Wyszukujesz frazę "Han, J. Y." wg kryterium: Autor

  Lista filtrów
Wyrównaj
Wyświetlanie 1-15 z 26
Tytuł:
Wzrost i właściwości optyczne kryształu BaGd2 (MoO4)4 domieszkowanego Er3+
Growth and optical properties of Er3+-doped BaGdcrystal 2 (MoO4)4
Autorzy:
Han, S.
Wang, J.
Zhang, H.
Pan, S.
Xv, H.
Wang, Y.
Shen, C.
Powiązania:
https://bibliotekanauki.pl/articles/142191.pdf
Data publikacji:
2013
Wydawca:
Stowarzyszenie Inżynierów i Techników Przemysłu Chemicznego. Zakład Wydawniczy CHEMPRESS-SITPChem
Tematy:
Wytwarzanie kryształów
ciepło właściwe
widmo
czas trwania fluorescencji
crystal growth
specific heat
spectrum
fluorescence lifetime
Opis:
Pojedynczy kryształ BaGd2(MoO4)4 domieszkowany 1% at. Er3+ wyhodowano metodą Czochralskiego. Omówiono szczegóły procedury otrzymywania i wzrostu kryształu. Kryształ ma doskonałą płaszczyznę poślizgu (010), a jego łupliwość czyni go przydatnym jako ośrodek czynny w mikrolaserach. Zmierzono widmo absorpcyjne w zakresie światła widzialnego i bliskiej podczerwieni (NIR) w temperaturze pokojowej. W zakresie od 380 do 1600 nm występuje kilka intensywnych pików absorpcyjnych. Zmierzono również widmo fluorescencyjne wzbudzane za pomocą lampy ksenonowej. Zaobserwowano intensywny pik emisyjny NIR 1536 nm. Czasy trwania fluorescencji 4I13/2 oraz 4I11/2 wyznaczone za pomocą dopasowania krzywej wykładniczej wyniosły odpowiednio 5,85 ms i 112,62 μs. Ciepło właściw Er3+ BaGd2(MoO4)4 w 25°C wynosi 0,471 J g-1 K-1. Na podstawie zmierzonych widm obliczono parametry optyczne na podstawie teorii Judda-Ofelta (J–O).
A 1 at % Er3+ doped BaGd2(MoO4)4 single crystal was grown by the Czochralski method. Details on the preparation and growth procedures were discussed. The crystal has a perfect (010) cleavage plane, and the cleavage character makes the crystal suitable as a gain medium for microchip lasers. The absorption spectrum in the visible and near-infrared (NIR) regions was measured at room temperature. There are several strong absorption peaks in the range from 380 to 1600 nm. The fluorescence spectrum excited by a Xenon lamp was also measured. A strong NIR emission peak located at 1536 nm was observed. The fluorescence lifetimes of 4I13/2 and 4I11/23+:BaGd2(MoO4)4 at 25°C is 0.471 J g-1 K-1. Using the measured spectra, the optical parameters were calculated using the J–O theory.
Źródło:
Chemik; 2013, 67, 9; 763-770
0009-2886
Pojawia się w:
Chemik
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Interaction of Water with Glycine: A Combined Inelastic Neutron Scattering and Raman Spectra Studies
Autorzy:
Zhang, P.
Zhang, Y.
Han, S.
Yan, Q.
Li, J.
Powiązania:
https://bibliotekanauki.pl/articles/2044762.pdf
Data publikacji:
2006-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
36.20.Ng
87.64.Je
25.40.Fq
82.30.Rs
Opis:
The vibrational dynamics of water around glycine was investigated by using Raman spectroscopy and inelastic neutron scattering. Experiments of deuterated glycine versus deuterium were performed as comparison. The study shows that for glycine, the exchange of proton-deuteron on the active NH$\text{}_{3}^{+}$ side was easy, whereas there was hardly exchange on the CH$\text{}_{2}$ side. Comparing different proportion of glycine vs. water molecules we obtained that the presence of water hardly changes the main features of glycine illustrating its hydrophobic character. The intralayer hydrogen bonds of glycine crystal are difficult to be replaced due to its stronger bond than water.
Źródło:
Acta Physica Polonica A; 2006, 109, 3; 399-404
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study on Ships Safety Control System
Autorzy:
Han, X. J.
Meng, X. Y.
Wang, Z. W.
Powiązania:
https://bibliotekanauki.pl/articles/117442.pdf
Data publikacji:
2009
Wydawca:
Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
Tematy:
Safety of Navigation
ships safety
Control System
Ships Safety Control System
Ship Accidents
Human Factor
Control Strategy
Accident Precaution
Opis:
The proportion of main reasons of ships accidents to the whole reasons is discussed in the paper. Among these reasons, human factors are in the majority. So a method to prevent wrong orders sent by a navigator is laid emphasis on. On the basis of this, a ships safety control system is studied. The construction and control principle of the ships safety control system, as well as control strategy, implementation method and key technology are elaborated in the paper.
Źródło:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2009, 3, 1; 19-22
2083-6473
2083-6481
Pojawia się w:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study on Effects of Mold Temperature on the Injection Molded Article
Autorzy:
Han, J.-H.
Kim, Y.-C.
Powiązania:
https://bibliotekanauki.pl/articles/351919.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
injection molding
mold temperature
cooling temperature
molded article
Opis:
This is a study of the effects of temperature of injection mold on the injection molded article. By supplying water of the proper temperature in the cooling line of mold in the cooling process, the mold was the appropriate temperature, and the deformation of the injection molded article was examined according to the mold temperature. In this study, we conducted simulation analysis and experiments, and the results were analyzed. The minimum deformation of the injection molded article model obtained by supplying 50°C water in the cooling line is 0.003 mm, and the maximum deformation was 0.813 mm. Injection molded article models obtained by supplying 20°C water were found to be a minimum of 0.002 mm, with deformation of up to 0.761 mm. When comparing both conditions, the error rate of injection molded article obtained by supplying 20°C water in the mold cooling line was lower by about 0.18%.
Źródło:
Archives of Metallurgy and Materials; 2017, 62, 2B; 1271-1274
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Simultaneous removal of SO2 and NOx from sintering flue gas using ammonia-Fe(II)EDTA combined with electrolytic regeneration
Autorzy:
Liang, Y.
Yao, X.
Quin, L.
Chen, W.
Han, J.
Powiązania:
https://bibliotekanauki.pl/articles/208117.pdf
Data publikacji:
2018
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
ammonia
desulfurization
flue gases
nitrogen oxides
nitrogen removal
sintering sulfur dioxide
amoniak
odsiarczanie
gazy odlotowe
tlenki azotu
usuwanie azotu
spiekanie
ditlenek siarki
Opis:
Sulfur dioxide and nitrogen oxide are health hazardous gases, which contribute to the formation of submicron acidic particulates. To reduce SO2 and NOx emission from the sintering flue gas, the combination of ammonia-Fe(II)EDTA solution scrubbing with Fe(III) electrolytic regeneration is proposed. The above method has the following advantages: direct conversion of NOx and SO2 to harmless N2 and SO4 2−, recovery of the by-product (NH4)2SO4), simultaneous removal of NOx and SO2 emission from flue gas in the reactor. The effect of the pH, initial Fe(II)EDTA concentration, and voltage on the desulfurization and denitration efficiencies was investigated using a bench-scale reactor. The maximal desulfurization and denitration efficiencies were 98% and 52%, respectively. The optimum parameters were pH ˃ 5.0, 2.1 V, and 0.05 mol·dm–3 Fe(II)EDTA concentration. SO2 and NOx removal from the sintering flue gas by ammonia-Fe(II)EDTA solution scrubbing combined with electrolytic regeneration was also demonstrated in a pilot-scale reactor.
Źródło:
Environment Protection Engineering; 2018, 44, 2; 19-36
0324-8828
Pojawia się w:
Environment Protection Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Selective reduction of PbSO4 to PbS with carbon and flotation treatment of synthetic galena
Autorzy:
Zheng, Y.-X.
Liu, W.
Qin, W.-Q.
Han, J.-W.
Yang, K.
Luo, H.-L.
Powiązania:
https://bibliotekanauki.pl/articles/110717.pdf
Data publikacji:
2015
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
wastes
lead sulfate
reduction
carbon
lead sulfide
flotation
Opis:
In order to recover lead from the zinc leaching residues, a new technology involving selective reduction of lead sulfate to lead sulfide with carbon followed by flotation was investigated. The reduction thermodynamics of PbSO4 was discussed and the effects of molar ratio of C to PbSO4, reaction temperature and time were examined by thermogravimetry (TG) and XRD. Verification tests were further carried out to prove the conclusions of thermodynamic and TG analyses, and the transformation extent could reach 86.45% under the optimal roasting conditions. The prepared galena was then subjected to micro-flotation tests, and the highest lead recovery could reach up to 75.32%.
Źródło:
Physicochemical Problems of Mineral Processing; 2015, 51, 2; 535-546
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Reduction of Thermal Conductivity Through Complex Microstructure by Dispersion of Carbon Nanofiber in p-Type Bi0.5Sb1.5Te3 Alloys
Autorzy:
Sharief, P.
Madavali, B.
Sohn, Y.
Han, J. H.
Song, G.
Hong, S. J.
Powiązania:
https://bibliotekanauki.pl/articles/2049341.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bismuth telluride
carbon nano fiber
grain size
Thermal conductivity
ZT
Opis:
The influence of nano dispersion on the thermoelectric properties of Bi2Te3 was actively investigating to wide-spread thermoelectric applications. Herein this report, we have systematically controlled the microstructure of Bi0.5Sb1.5Te3 (BST) alloys through the incorporation of carbon nanofiber (CNF), and studied their effect on thermoelectric properties, and mechanical properties. The BST/x-CNF (x-0, 0.05, 0.1, 0.2 wt.%) composites powder was fabricated using high energy ball milling, and subsequently consolidated the powder using spark plasma sintering. The identification of CNF in bulk composites was analyzed in Raman spectroscopy and corresponding CNF peaks were recognized. The BST matrix grain size was greatly reduced with CNF dispersion and consistently decreased along CNF percentage. The electrical conductivity was reduced and Seebeck coefficient varied in small-scale by embedding CNF. The thermal conductivity was progressively diminished, obtained lattice thermal conductivity was lowest compared to bare sample due to induced phonon scattering at interfaces of secondary phases as well as highly dense fine grain boundaries. The peak ZT of 0.95 achieved for 0.1 wt.% dispersed BST/CNF composites. The Vickers hardness value of 101.8 Hv was obtained for the BST/CNF composites.
Źródło:
Archives of Metallurgy and Materials; 2021, 66, 3; 803-808
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prediction of the height of overburden fractured zone in deep coal mining: case study
Prognozowanie wysokości strefy spękań w warstwach nadkładu w podziemnych kopalniach węgla: studium przypadku
Autorzy:
Han, Y.
Cheng, J.
Huang, Q.
Zou, D. H. S.
Zhou, J.
Huang, S.
Long, Y.
Powiązania:
https://bibliotekanauki.pl/articles/218745.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
wysokość strefy spękań w warstwie nadkładu
symulacje numeryczne
układ ortogonalny
pomiary in-situ
the hei ght of overburden fractured zone
numerical simulation
orthogonal design
in-situ test
Opis:
In the process of coal extraction, a fractured zone is developed in the overburden above the goaf. If the fractured zone is connected with an aquifer, then water inrush may occur. Hence, research and analysis of the height of overburden fractured zone (HOFZ) are of considerable significance. This study focuses on the HOFZ determination in deep coal mining. First, general deformation failure characteristics of overburden were discussed. Second, a new method, numerical simulation by orthogonal design(NSOD), have been proposed to determinate the HOFZ in deep coal mining. Third, the validity of NSOD is verified in the practical application, compared with empiric al formula in Chinese Regulations and in-situ test. These three methods were applied to determine the HOFZ of working face No. 111303 in No. 5 coal mine. The pre dicted HOFZ of NSOD is found to be similar to the result of the in-situ test (8.9% relative error), whereas the HOFZ calculated by the empirical formula has extremely large error (25.7% relative error). Results show that the NSOD can reliably predict the HOFZ in deep coal mining and reduce time and expenses required for in-situ test.
W trakcie prowadzenia wydobycia węgla w warstwie skalnej leżącej ponad zrobami powstaje strefa spękań. Jeśli nieciągłości te związane są z formacjami wodonośnymi, może nastąpić nagły wypływ wód do kopalni. Stąd też waga problemu i konieczność badania wysokości strefy spękań w warstwach nadkładu zalegających nad wyrobiskiem. W pracy tej główny nacisk położono na określenie wysokości strefy spękań warstwy nadkładu zalegającej ponad wyrobiskiem w kopalni podziemnej. W pierwszej części pracy przedstawiono główne charakterystyki powstawania deformacji i pęknięć w warstwach nadkładu. Następnie zaproponowano nową metodę symulacji numerycznych w układzie ortogonalnym i jej wykorzystanie do określania wysokości strefy spękań w warstwach nadkładu w kopalni podziemnej. Następnie powyższą metodę zweryfikowano w praktycznym zastosowaniu, jako poziomy odniesienia wykorzystano odpowiednie wzory empiryczne określone w przepisach obowiązujących w Chinach oraz wyniki pomiarów in-situ. Powyższe trzy metody zastosowane zostały do określenia wysokości strefy spękań w warstwach nadkładu przy prowadzeniu ściany 111303 w kopalni węgla nr 5. Wyniki obliczeń wysokości strefy spękań uzyskane w oparciu o zaproponowaną nową metodę w dużym stopniu pokrywały się z wynikami pomiarów in-situ (błąd względny 8.9%); podczas gdy obliczenia wysokości strefy spękań w oparciu o odpowiednie wzory empiryczne obarczone były dużym błędem (błąd względny 25.7%). Uzyskane wyniki wskazują wiarygodność obliczeń w oparciu o zaproponowaną metodę, co pozwoli na ograniczenie czasu i kosztów związanych z wykonywaniem pomiarów in-situ.
Źródło:
Archives of Mining Sciences; 2018, 63, 3; 617-631
0860-7001
Pojawia się w:
Archives of Mining Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Oligonol prevented the relapse of dextran sulfate sodium-ulcerative colitis through enhancing Nrf2-mediated antioxidative defense mechanism
Autorzy:
Kim, K.-J.
Park, J.-M.
Lee, J.-S.
Kim, Y.S.
Kangwan, N.
Han, Y.-M.
Kang, E.A.
An, J.M.
Park, Y.K.
Hahm, K.-B.
Powiązania:
https://bibliotekanauki.pl/articles/69501.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Fizjologiczne
Tematy:
ulcerative colitis
inflammatory bowel disease
oxidative stress
oligonol
polyphenol
adaptive response
quinone oxidoreductase
relapse
nuclear factor-kappaB
tumour necrosis factor-alpha
Źródło:
Journal of Physiology and Pharmacology; 2018, 69, 3
0867-5910
Pojawia się w:
Journal of Physiology and Pharmacology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Morphology Change Of Si Deposit In Molten Salt Electrorefining
Zmiana morfologii osadu Si podczas elektrorafinacji w stopionych solach
Autorzy:
Ryu, H. Y.
Kwon, S. C.
Han, M. H.
An, Y. S.
Lee, J. S.
Lee, J. H.
Powiązania:
https://bibliotekanauki.pl/articles/354346.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
molten salt
silicon
morphology
electrorefining
current density
sól stopiona
krzem
morfologia
elektrorafinacja
gęstość prądu
Opis:
The effects of processing parameters on the morphology change in a Si deposit recovered by means of molten salt electrorefining are evaluated using electrochemical techniques such as cyclic voltammetry and chronopotentiometry at 800°C. It was found that concentration of K2SiF6 and current density were important parameters in determining deposit size. Higher concentrations of K2SiF6 were effective in coarsening the silicon deposit and decreasing the cell potential. Silicon nanofiber was recovered at 5 wt% of K2SiF6 whereas dense particles were prepared at 30 and 50 wt% of K2SiF6. The morphology of the Si deposit was determined by the concentration of Si in the electrolyte which is related to the formation of crystal and growth of Si. The formation mechanism of the Si deposit was interpreted by using high resolution TEM as well as electrochemical properties.
Źródło:
Archives of Metallurgy and Materials; 2015, 60, 2B; 1491-1497
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Improved Mechanical Properties of Al-Si Alloys by Adding an Extrusion Process to Conventional Processing Method
Autorzy:
Han, J. Y.
Femi, O. E.
Kilicaslan, F.
Jeong, C. U.
Baeg, C. H.
Hong, S. J.
Koo, J. M.
Powiązania:
https://bibliotekanauki.pl/articles/355114.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Al-Si alloys
extrusion
mechanical properties
Opis:
In this study, the mechanical properties of Al-Si alloys was improved by selecting higher Si content and avoiding distortion in Al-Si matrix by adding an extrusion process such that the fabrication processes casting and extrusion were in sequence. Cast billets were extruded at ratios 4:1 and 10:1 to obtained two extruded bars with different processing parameters. The as-extruded samples were characterized and investigated for microstructure and mechanical properties. Optical Microscope was used to examined phase morphologies and microstructures of the extruded Al-Si bars. Mechanical properties were conducted on each sample to study the effectiveness of the additional extrusion process in high Si content Al-Si alloys. The result shows that by increasing the extrusion ratio, the size of the primary Si particle reduces leading to a higher density, better tensile and yield strength compared to the cast billet.
Źródło:
Archives of Metallurgy and Materials; 2017, 62, 2B; 1179-1183
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of medicinal plant Schisandra chinensis using a potential DNA barcode ITS2
Autorzy:
Li, X.-K.
Wang, B.
Han, R.-C.
Zheng, Y.-C.
Yin, H.-B.
Xu, L.
Zhang, J.-K.
Xu, B.-L.
Powiązania:
https://bibliotekanauki.pl/articles/56996.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
identification
internal transcribed spacer
medicinal plant
Schisandra chinensis
DNA barcode
Opis:
To test whether the internal transcribed spacer 2 (ITS2) region is an effective marker for using in authenticating of the Schisandra chinensis at the species and population levels, separately. And the results showed that the wild populations had higher percentage of individuals that had substitution of C→A at site 86-bp than the cultivated populations. At sites 10-bp, 37-bp, 42-bp and 235-bp, these bases of the Schisandra sphenanthera samples differed from that of S. chinensis. Two species showed higher levels of inter-specific divergence than intra-specific divergence within ITS2 sequences. However, 24 populations did not demonstrate much difference as inter-specific and intra-specific divergences were concerned. Both S. chinensis and S. sphenanthera showed monophyly at species level, yet the samples of different populations shown polyphyly at population level. ITS2 performed well when using BLAST1 method. ITS2 obtained 100% identification success rates at the species level for S. chinensis, with no ambiguous identification at the genus level for ITS2 alone. The ITS2 region could be used to identify S. chinensis and S. sphenanthera in the “Chinese Pharmacopoeia”. And it could also correctly distinguish 100% of species and 100% of genera from the 193 sequences of S. chinensis. Hence, the ITS2 is a powerful and efficient tool for species identification of S. chinensis.
Źródło:
Acta Societatis Botanicorum Poloniae; 2013, 82, 4
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
Autorzy:
Dai, G.
Huang, X.
Liu, Q.
Li, Y.
Zhang, L.
Han, K.
Yang, J.
Liu, Y.
Xue, F.
Zhao, D.
Powiązania:
https://bibliotekanauki.pl/articles/16647377.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
epitope
goose astrovirus
capsid protein
monoclonal antibody
Opis:
Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Generation and characterization of a monoclonal antibody against duck Tembusu virus envelope protein
Autorzy:
Han, K.
Zhao, D.
Liu, Y.
Liu, Q.
Huang, X.
Yang, J.
Bi, K.
Xu, T.
Li, Y.
Powiązania:
https://bibliotekanauki.pl/articles/31495.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused massive economic losses to the duck industry in China. Envelope (E) protein of DTMUV is an important structural protein, which is able to induce protective immune response in target animals and can be used as specific serological diagnosis tool. In this study, a novel monoclonal antibody, designated mAb 3E9, was generated against DTMUV E protein. It is positive in indirect ELISA against both His-E protein and the purified whole viral antigen. Also, this mAb showed positive reaction with DTMUV in Western blot and indirect immunofluorescence assay, and the isotype was IgG1. End-point neutralizing assay performed in BHK-21 cells revealed that the neutralization titer of 3E9 against DTMUV JS804 strain reached 1:50. Furthermore, functional studies revealed that 3E9 blocks infection of DTMUV at a step on viral attachment. The anti-E mAbs produced in the present work may be valuable in developing an antigen-capture ELISA test for antigen detection or a competitive ELISA test for antibody detection or therapeutic medicine for DTMUV in poultry.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 26

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies