- Tytuł:
- Effects of mismatches and insertions on discrimination accuracy of nucleic acid probes
- Autorzy:
-
Piao, Xianyu
Sun, Liancheng
Zhang, Tianbiao
Gan, Youlin
Guan, Yifu - Powiązania:
- https://bibliotekanauki.pl/articles/1040677.pdf
- Data publikacji:
- 2008
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
insertion
mismatch
melting temperature
hybridization stability - Opis:
- Effective discrimination of non-complementary nucleotides is an important factor to ensure the accuracy of hybridization-based nucleic acid analyses. The current study investigates the effects of the chemical nature, the positions, the numbers, and the cooperative behavior of mismatches as well as insertions on 20-mer and 30-mer duplexes. We observed the hybridization stability trend affected by mismatches: G:T ≈ G:G > G:A > A:A ≈ T:T > A:C ≈ T:C > C:C. The experimental data show that mismatches at the center of the oligonucleotide probes have a more profound destabilizing effect on the hybridization stability than those at either ends. Insertions also demonstrate a similar destabilizing effect as mismatches. These results provide useful information for designing DNA microarray nucleotide probes and for improving the discrimination accuracy of hybridization-based detections.
- Źródło:
-
Acta Biochimica Polonica; 2008, 55, 4; 713-720
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł