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Wyświetlanie 1-15 z 53
Tytuł:
Wykrywanie DNA Toxoplasma gondii w plynach ustrojowych metoda PCR
Autorzy:
Golab, E
Powiązania:
https://bibliotekanauki.pl/articles/837546.pdf
Data publikacji:
1995
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
metody badan
plyny ustrojowe
parazytologia
lancuchowa reakcja polimerazy
pasozyty
wykrywanie
DNA
Toxoplasma gondii
Opis:
The polymerase chain reaction (PCR) is used for in vitro amplification of specific DNA fragments. The PCR technique is based on the reiteration of a three-step process: denaturation DNA into single strands, annealing primers (specific synthetic oligonucleotides) to the DNA template and enzymatic extension from the primers along the templates. The usefulness of this new technique to the detection of Toxoplasma gondii DNA has been described.
Źródło:
Annals of Parasitology; 1995, 41, 1; 13-18
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wystepowanie DNA Pneumocystis carinii i Pneumocystis wakefieldiae w tkankach pozaplucnych szczurow laboratoryjnych poddawanych immunosupresji
Presence of Pneumocystis carinii and Pneumocystis wakefieldiae DNA in the extrapulmonary tissues of immunocompromised laboratory rats
Autorzy:
Golab, E
Powiązania:
https://bibliotekanauki.pl/articles/837669.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
szczury
wystepowanie
zwierzeta laboratoryjne
tkanki zwierzece
pasozyty
Pneumocystis wakefieldiae
Pneumocystis carinii
immunosupresja
DNA
Źródło:
Annals of Parasitology; 2009, 55, 2; 167-171
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Diagnostyka molekularna Pneumocystis carinii wobec nowych danych dotyczacych biologii i epidemiologii
Autorzy:
Golab, E
Powiązania:
https://bibliotekanauki.pl/articles/841258.pdf
Data publikacji:
1999
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
diagnostyka molekularna
grzyby pasozytnicze
choroby pasozytnicze
parazytologia
pneumocystoza
epidemiologia
Pneumocystis carinii
Opis:
A new data from investigations on Pneumocystis carinii were presented as well as an application of molecular techniques to the diagnosis of P. carinii pneumonia.
Źródło:
Annals of Parasitology; 1999, 45, 3; 405-409
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Diagnostyka molekularna Pneumocystis carinii wobec nowych danych dotyczących biologii i epidemiologii
THE MOLECULAR DIAGNOSIS OF PNEUMOCYSTIS CARINII IN THE LIGHT OF A NEW - BIOLOGICAL AND EPIDEMIOLOGICAL DATA
Autorzy:
Gołąb, E.
Powiązania:
https://bibliotekanauki.pl/articles/2148830.pdf
Data publikacji:
1999
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
diagnostyka molekularna
grzyby pasozytnicze
choroby pasozytnicze
parazytologia
pneumocystoza
epidemiologia
Pneumocystis carinii
Opis:
A new data from investigations on Pneumocystis carinii were presented as well as an application of molecular techniques to the diagnosis of P. carinii pneumonia.
Źródło:
Wiadomości Parazytologiczne; 1999, 45, 3; 405-409
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wykrywanie DNA Toxoplasma gondii w płynach ustrojowych metodą PCR
DETECTION OF TOXOPLASMA GONDII DNA IN BODY FLUIDS BY POLYMERASE CHAIN REACTION (PCR)
Autorzy:
Gołąb, E.
Powiązania:
https://bibliotekanauki.pl/articles/2151376.pdf
Data publikacji:
1995
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
metody badan
plyny ustrojowe
parazytologia
lancuchowa reakcja polimerazy
pasozyty
wykrywanie
DNA
Toxoplasma gondii
Opis:
The polymerase chain reaction (PCR) is used for in vitro amplification of specific DNA fragments. The PCR technique is based on the reiteration of a three-step process: denaturation DNA into single strands, annealing primers (specific synthetic oligonucleotides) to the DNA template and enzymatic extension from the primers along the templates. The usefulness of this new technique to the detection of Toxoplasma gondii DNA has been described.
Źródło:
Wiadomości Parazytologiczne; 1995, 41, 1; 13-18
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Występowanie DNA Pneumocystis carinii i Pneumocystis wakefieldiae w tkankach pozapłucnych szczurów laboratoryjnych poddawanych immunosupresji
Presence of Pneumocystis carinii and Pneumocystis wakefieldiae DNA in the extrapulmonary tissues of immunocompromised laboratory rats
Autorzy:
Gołąb, E.
Powiązania:
https://bibliotekanauki.pl/articles/2143539.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
szczury
wystepowanie
zwierzeta laboratoryjne
tkanki zwierzece
pasozyty
Pneumocystis wakefieldiae
Pneumocystis carinii
immunosupresja
DNA
Opis:
Fungi of the genus Pneumocystis are opportunistic pathogens which cause lethal pneumonia in immunocompromised hosts. Those fungi may also invade other visceral organs where they induce lesions, although, the pathways or mechanisms of the in vivo infection are still unknown. The corticosteroid-treated rat model was used to evaluate the course of extrapulmonary pneumocystosis. Liver, kidney, spleen, and mesenteric lymph nodes of 16 rats were examined for the presence of mtLSU gene fragments of P. carinii and P. wakefieldiae using the nested PCR method. Pneumocystis DNA was detected in 26 organ samples of which 17 contained both species (P. carinii and P. wakefieldiae) and 9 contained only P. carinii. Positive samples were received from 10 rats examined after 6–9 weeks of immunosuppression. The highest percentage of positive samples (62.5%) was obtained among examined visceral lymph nodes. Pneumocystis DNA was detected in the blood serum of two rats with no traces of the DNA in their internal organs. Conversely, Pneumocystis DNA was found in the internal organs of two other rats, although their serum samples were negative. The average number of Pneumocystis cysts in the lungs of animals in which extrapulmonary infection was detected was 3.4 per one microscopic view field. In the case of animals where the infection was limited to the lung tissue this number was almost two times lower (1.8 cysts per one microscopic view field). An analysis of the results of the presently reported experiment showed that massive Pneumocystis infection in the lungs makes it more likely that Pneumocystis will spread to other internal organs. This spread probably takes place via the lymphatic vessels. The extrapulmonary foci may contain either P. carinii alone, or both pathogens: P. carinii and P. wakefieldiae.
Źródło:
Wiadomości Parazytologiczne; 2009, 55, 2; 167-171
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Diagnosing Toxoplasma gondii infection during pregnancy - a case study
Autorzy:
Karczewski, G.
Golab, E.
Powiązania:
https://bibliotekanauki.pl/articles/5842.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
diagnosis
human disease
Toxoplasma gondii
infection
pregnancy
toxoplasmosis
Polska
Źródło:
Annals of Parasitology; 2016, 62, Suppl.
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Epidemiologia wlosnicy w Polsce dawniej i dzis
Epidemiology of human trichinellosis in Poland - currently and in the past
Autorzy:
Golab, E
Sadkowska-Todys, M.
Powiązania:
https://bibliotekanauki.pl/articles/841256.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wlosnica
zagrozenia zdrowia
historia
Polska
pasozyty
nicienie
epidemiologia
Warszawa konferencja
wystepowanie
choroby czlowieka
parazytologia
konferencje
Trichinella
Opis:
Since the XIX century human trichinellosis has remained an unsolved problem of public healthcare in Poland. This paper describes the past situation and analyses current changes in the epidemiological pattern of trichinellosis in Poland. Epidemiological data from the last 60 years, point out that the number of human cases as well as the number of deaths caused by trichinellosis has decreased significantly. Up to 90s the main source of Trichinella infection for people was pork. Among other implemented control measures, the introduction of the artificial digestion method in the early 80s to detect trichinellosis in pigs resulted in a shift in the sources of Trichinella infection in humans - pork was replaced with wild boar meat. In the years 1990-1995 the number of outbreaks due to pork consumption was 3.5-times higher than in the years 2000-2005. In the early nineties pork was the source of infection causing about 71% of all outbreaks; in 2000-2005 that number has fallen to only 12%. On the other hand wild boar meat was responsible for 23% of the outbreaks in 1990-1995 and as many as 88% of all outbreaks in the years 2000-2005. Moreover the number of persons infected in the outbreaks significantly decreased. The study of wild animals demonstrated that wild boars in Poland are infected not only with T. spiralis but also with Trichinella britovi. These results and EU recommendations indicate a requirement of determining the Trichinella species which cause infections in outbreaks. In the 3 trichinellosis outbreaks in 2005 the infected meat products were examined with molecular tools. T. spiralis species larvae were the etiological agents of infection in all these outbreaks. The current epidemiological situation of trichinellosis in Poland indicates a need of increasing the awareness of risks related to wild boar meat consumption among the general public. Introducing the artificial digestion method as an obligatory method for wild boar meat examination is also necessary.
Źródło:
Annals of Parasitology; 2006, 52, 3; 181-187
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Epidemiologia włośnicy w Polsce dawniej i dziś
Epidemiology of human trichinellosis in Poland - currently and in the past
Autorzy:
Gołąb, E.
Sadkowska-Todys, M.
Powiązania:
https://bibliotekanauki.pl/articles/2144340.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wlosnica
zagrozenia zdrowia
historia
Polska
pasozyty
nicienie
epidemiologia
Warszawa konferencja
wystepowanie
choroby czlowieka
parazytologia
konferencje
Trichinella
Opis:
Since the XIX century human trichinellosis has remained an unsolved problem of public healthcare in Poland. This paper describes the past situation and analyses current changes in the epidemiological pattern of trichinellosis in Poland. Epidemiological data from the last 60 years, point out that the number of human cases as well as the number of deaths caused by trichinellosis has decreased significantly. Up to 90s the main source of Trichinella infection for people was pork. Among other implemented control measures, the introduction of the artificial digestion method in the early 80s to detect trichinellosis in pigs resulted in a shift in the sources of Trichinella infection in humans - pork was replaced with wild boar meat. In the years 1990-1995 the number of outbreaks due to pork consumption was 3.5-times higher than in the years 2000-2005. In the early nineties pork was the source of infection causing about 71% of all outbreaks; in 2000-2005 that number has fallen to only 12%. On the other hand wild boar meat was responsible for 23% of the outbreaks in 1990-1995 and as many as 88% of all outbreaks in the years 2000-2005. Moreover the number of persons infected in the outbreaks significantly decreased. The study of wild animals demonstrated that wild boars in Poland are infected not only with T. spiralis but also with Trichinella britovi. These results and EU recommendations indicate a requirement of determining the Trichinella species which cause infections in outbreaks. In the 3 trichinellosis outbreaks in 2005 the infected meat products were examined with molecular tools. T. spiralis species larvae were the etiological agents of infection in all these outbreaks. The current epidemiological situation of trichinellosis in Poland indicates a need of increasing the awareness of risks related to wild boar meat consumption among the general public. Introducing the artificial digestion method as an obligatory method for wild boar meat examination is also necessary.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 3; 181-187
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analiza przebiegu doswiadczalnej pneumocystozy u szczurow za pomoca wybranych metod serologicznych
Autorzy:
Sobolewska, A
Bitkowska, E.
Golab, E.
Powiązania:
https://bibliotekanauki.pl/articles/836941.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
szczury
choroby pasozytnicze
metody serologiczne
ocena kliniczna
zwierzeta doswiadczalne
parazytologia
pneumocystoza
przebieg choroby
Opis:
Since serological methods used in the diagnosis of pneumocystosis may be helpful to a various extent, depending on the stage of a diagnosed infection, it was decided to evaluate the usefulness of some the methods in the course of infection. Wistar rats were used in the experiments. The animals were administered hydrocortisone infections for 12 weeks to induce immunosuppression and activate naturally occurring asymptomatic infections with Pneumocystis carinii. Blood samples and specimes of lung tissues were collected, then they were examined for the presence of specific IgG and IgM antibodies, circulating antigen of Pneumocystsis carinii and circulating immune complexes using immunoenzymatic assays. The results of the above experiments indicated, that in an early stage of infection, the examinations of serum samples for circulating immune complexes were helpful, particularly for these with IgM antibodies.
Źródło:
Annals of Parasitology; 2001, 47, 4; 717-721
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wystepowanie DNA Pneumocystis carinii i przeciwcial pneumocystozowych w surowicach niemowlat w okresie fizjologicznego spadku odpornosci
Autorzy:
Golab, E
Sobolewska, A.
Matysiak, E.
Powiązania:
https://bibliotekanauki.pl/articles/838099.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
niemowleta
surowica krwi
grzyby pasozytnicze
wystepowanie
immunoglobulina G
przeciwciala
immunoglobulina M
parazytologia
Pneumocystis carinii
okres fizjologicznego spadku odpornosci
DNA
Opis:
Occurrence of Pneumocystis carinii DNA aod anti-Pneumocystis antibodies in sera of infants at the age of humoral response natural degradation. The polymerase chain reaction (PCR) and indirect immunofluorescent test (IF) were used for examination of serum samples obtained from infants with respiratory tract infections. Sixty (11,9%) out of the 503 examined infant samples were positive for anti-P. carinii IgM and 354 (70,4%) contained anti-Pneumocystis IgG. P. carinii DNA was found in 6 (6,7%) sera from 90 of infected infants. Five out these 6 samples were for anti-Pneumocystis antibodies positive; 4 contained both IgG and IgM classes and one bad only IgG. The sixth sample had neither IgG nor IgM, despite of P. carinii DNA presence. The results of the studies indicated that for diagnosis Pneumocystis carinii pneumonia (PCP) in infants on serum specimens detection of antibodies by IF test is of greater value than Pneumocystis DNA amplification by PCR method.
Źródło:
Annals of Parasitology; 2002, 48, 3; 287-292
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Występowanie DNA Pneumocystis carinii i przeciwciał pneumocystozowych w surowicach niemowląt w okresie fizjologicznego spadku odporności
Autorzy:
Gołąb, E.
Sobolewska, A.
Matysiak, E.
Powiązania:
https://bibliotekanauki.pl/articles/2147739.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
niemowleta
surowica krwi
grzyby pasozytnicze
wystepowanie
immunoglobulina G
przeciwciala
immunoglobulina M
parazytologia
Pneumocystis carinii
okres fizjologicznego spadku odpornosci
DNA
Opis:
Occurrence of Pneumocystis carinii DNA aod anti-Pneumocystis antibodies in sera of infants at the age of humoral response natural degradation. The polymerase chain reaction (PCR) and indirect immunofluorescent test (IF) were used for examination of serum samples obtained from infants with respiratory tract infections. Sixty (11,9%) out of the 503 examined infant samples were positive for anti-P. carinii IgM and 354 (70,4%) contained anti-Pneumocystis IgG. P. carinii DNA was found in 6 (6,7%) sera from 90 of infected infants. Five out these 6 samples were for anti-Pneumocystis antibodies positive; 4 contained both IgG and IgM classes and one bad only IgG. The sixth sample had neither IgG nor IgM, despite of P. carinii DNA presence. The results of the studies indicated that for diagnosis Pneumocystis carinii pneumonia (PCP) in infants on serum specimens detection of antibodies by IF test is of greater value than Pneumocystis DNA amplification by PCR method.
Źródło:
Wiadomości Parazytologiczne; 2002, 48, 3; 287-292
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Improved Blastocystis spp. detection method using swabs with Amies transport medium and charcoal
Autorzy:
Kaczmarek, A.
Gołąb, E.
Sałamatin, R.
Powiązania:
https://bibliotekanauki.pl/articles/28761825.pdf
Data publikacji:
2022
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2022, 29, 2; 303-305
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analiza przebiegu doświadczalnej pneumocystozy u szczurów za pomocą wybranych metod serologicznych
AN ANALYSIS OF THE COURSE OF EXPERIMENTAL PNEUMOCYSTOSIS IN RATS USING SOME SEROLOGICAL METHODS
Autorzy:
Sobolewska, A.
Bitkowska, E.
Gołąb, E.
Powiązania:
https://bibliotekanauki.pl/articles/2148143.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
szczury
choroby pasozytnicze
metody serologiczne
ocena kliniczna
zwierzeta doswiadczalne
parazytologia
pneumocystoza
przebieg choroby
Opis:
Since serological methods used in the diagnosis of pneumocystosis may be helpful to a various extent, depending on the stage of a diagnosed infection, it was decided to evaluate the usefulness of some the methods in the course of infection. Wistar rats were used in the experiments. The animals were administered hydrocortisone infections for 12 weeks to induce immunosuppression and activate naturally occurring asymptomatic infections with Pneumocystis carinii. Blood samples and specimes of lung tissues were collected, then they were examined for the presence of specific IgG and IgM antibodies, circulating antigen of Pneumocystsis carinii and circulating immune complexes using immunoenzymatic assays. The results of the above experiments indicated, that in an early stage of infection, the examinations of serum samples for circulating immune complexes were helpful, particularly for these with IgM antibodies.
Źródło:
Wiadomości Parazytologiczne; 2001, 47, 4; 717-721
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Demonstration of a soluble toxocaral antigen in the sera of naturally infected men and experimental rabbits
Autorzy:
Dzbenski, T.H.
Bitkowska, E.
Golab, E.
Powiązania:
https://bibliotekanauki.pl/articles/837550.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
parasite
infected rabbit
antigen
serum
man
circulating antigen
rabbit
Toxocara canis
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 53

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