- Tytuł:
- High salt-solvent systems in separation of betanin and its derivatives from red beet (Beta vulgaris L.) by high-performance countercurrent chromatography (HPCCC)
- Autorzy:
-
Spórna-Kucab, A.
Ignatova, S,
Garrard, I.
Wybraniec, S. - Powiązania:
- https://bibliotekanauki.pl/articles/115564.pdf
- Data publikacji:
- 2012
- Wydawca:
- Fundacja na Rzecz Młodych Naukowców
- Tematy:
-
betanin
betalains
betacyanins
countercurrent chromatography
Beta vulgaris L. - Opis:
- A study on a separation of betanin and its decarboxy- and dehydro-derivatives obtained from red beet roots (Beta vulgaris L.) using analytical high-performance countercurrent chromatography (HPCCC — Dynamic Extractions Ltd., UK) was performed. The HPCCC process was accomplished in the ‘tail to head’ mode with three highly polar solvent systems with high salt concentrations: 1-propanol-acetonitrile-saturated ammonium sulphate-water (v/v/v/v, 1:0.5:1.2:1); ethanol-acetonitrile-1- propanol-saturated ammonium sulphate-water (v/v/v/v/v, 0.5:0.5:0.5:1.2:1) and ethanol-1-butanol-acetonitrile-saturated ammonium sulphate-water (volume ratio), 0.5:0.5:0.5:1.2:1). HPLC analysis was performed in a conventional reversed phase mode with diode-array (DAD) detection to characterize the composition of obtained fractions. The applied solvent systems enabled the separation of the betalain pigments with high efficiency for the first time. In the mode of separation selected, the more hydrophobic compounds eluted first as expected. Moreover, for the first time, the applied HPCCC solvent systems generated a separation of 2-decarboxy-betanin from 17- and 2,17-bidecarboxy-betanin as well as from neobetanin and betanin.
- Źródło:
-
Challenges of Modern Technology; 2012, 3, 1; 57-61
2082-2863
2353-4419 - Pojawia się w:
- Challenges of Modern Technology
- Dostawca treści:
- Biblioteka Nauki
Artykuł