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    Wyświetlanie 1-6 z 6
    Tytuł:
    Isolation and characterization of novel human short-chain dehydrogenase/reductase SCDR10B which is highly expressed in the brain and acts as hydroxysteroid dehydrogenase*
    Autorzy:
    Huang, Chaoqun
    Wan, Bo
    Gao, Bo
    Hexige, Saiyin
    Yu, Long
    Powiązania:
    https://bibliotekanauki.pl/articles/1040586.pdf
    Data publikacji:
    2009
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    expression pattern
    clone
    brain
    hydroxysteroid dehydrogenase
    Opis:
    Hydroxysteroid dehydrogenase belongs to the subfamily of short-chain dehydrogenases/reductases (SDR), and 11-β-hydroxysteroid dehydrogenase catalyzes the interconversion of inactive glucocorticoids (cortisone in human, dehydrocorticosterone in rodents) and active glucocorticoids (cortisol in human, corticosterone in rodents). We report here the cloning and characterization of a novel human SDR gene SCDR10B which encodes a protein with similarity to 11β-hydroxysteroid dehydrogenase 1. SCDR10B was isolated from a human brain cDNA library, and was mapped to chromosome 19p13.3 by browsing the UCSC genomic database. It contains an ORF with a length of 858 bp, encoding a protein with a transmembrane helix and SDR domain. Its molecular mass and isoelectric point are predicted to be 30.8 kDa and 10.3 kDa, respectively. SCDR10B protein is highly conserved in mammals and fish. Phylogenetic tree analysis indicated that SCDR10B stands for a new subgroup in the 11β-hydroxysteroid dehydrogenase family. Northern blot analysis showed that SCDR10B was highly expressed in brain, and a strong expression signal was detected in hippocampal neurons by immunohistochemical analysis. RT-PCR and immunohistochemical analysis showed that SCDR10B was up-regulated in lung-cancer cell lines and human lung cancer. SCDR10B can catalyze the dehydrogenation of cortisol in the presence of NADP+, and therefore it is a hydroxysteroid dehydrogenase.
    Źródło:
    Acta Biochimica Polonica; 2009, 56, 2; 279-289
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Study of calculation method of pure mode II stress intensity factor of fine-grained concrete using different numerical models
    Autorzy:
    Zhou, Changlin
    Peng, Bo
    Deng, An
    Gao, Xiaofeng
    Li, Yaojia
    Zhou, Lei
    Zhu, Zheming
    Powiązania:
    https://bibliotekanauki.pl/articles/1839720.pdf
    Data publikacji:
    2021
    Wydawca:
    Polskie Towarzystwo Mechaniki Teoretycznej i Stosowanej
    Tematy:
    fine-grained concrete
    critical stress intensity factor
    J-integral
    anti-symmetrical four-point bending
    FEM
    XFEM
    Opis:
    Measuring and calculating methods of critical stress intensity factors (SIFs) have become hot topics which attracted large attention recently. In this work, anti-symmetrical four-point bending tests of cracked fine-grained concrete specimens were conducted experimentally and numerically by using a computer-controlled universal testing machine and ABAQUS code. A comparative study of the calculation method of pure mode II stress intensity factor of a fine-grained concrete was performed by utilizing the conventional finite element method (FEM) in two and three dimensions as well as the extended finite element method (XFEM) in three dimensions. The results show that in three-dimensional models, the crack mode is closest to the pure mode II at the center of specimen thickness. Pure mode II stress intensity factors obtained by SEAM2D and XFEM3D are 1.013 and 1.0617 times that by SEAM3D, respectively. Pure mode II stress intensity factors of the fine-grained concrete obtained by the conventional FEM are more stable than that by XFEM. The number of mesh circles has slight influence on the calculation results of pure mode II stress intensity factor.
    Źródło:
    Journal of Theoretical and Applied Mechanics; 2021, 59, 2; 251-264
    1429-2955
    Pojawia się w:
    Journal of Theoretical and Applied Mechanics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Improved flotation of auriferous arsenopyrite by using a novel mixed collector in weakly alkaline pulp
    Autorzy:
    Wang, Xiaohui
    Zhao, Kaile
    Bo, Hui
    Yan, Wu
    Wang, Zhen
    Gu, Guohua
    Gao, Zhiyong
    Powiązania:
    https://bibliotekanauki.pl/articles/1845220.pdf
    Data publikacji:
    2020
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    gold mine
    arsenopyrite
    mixed collector
    flotation
    Opis:
    The purpose of using a mixed collector is to increase both flotation efficiency and selectivity. The mixed collector of potassium isopentyldithiocarbonate and N-dodecyl mercaptan exhibits high efficiency for the flotation of auriferous arsenopyrite, and the 2:1 mixing mass ratio of potassium isopentyldithiocarbonate and N-dodecyl mercaptan is preferred. Batch flotation tests indicate that a concentrate with the grade of 47.58 g/Mg Au and the recovery of 86.45% Au is achieved by using the mixed potassium isopentyldithiocarbonate/N-dodecyl mercaptan in weakly alkaline pulp. The collector mixture potassium isopentyldithiocarbonate + N-dodecyl mercaptan has greater adsorption density on the arsenopyrite surface than other conventional mixed collectors. The mixed potassium isopentyldithiocarbonate/N-dodecyl mercaptan can adsorb onto the arsenopyrite surface by intense chemisorptions, and the Sulfur-Iron chemical complexation is considered as the main adsorption mode. This is the reason why mixed potassium isopentyldithiocarbonate and N-dodecyl mercaptan collector can improve the flotation efficiency of auriferous sulfides.
    Źródło:
    Physicochemical Problems of Mineral Processing; 2020, 56, 5; 996-1004
    1643-1049
    2084-4735
    Pojawia się w:
    Physicochemical Problems of Mineral Processing
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Newly identified transcripts of UL4 and UL5 genes of human cytomegalovirus
    Autorzy:
    Gao, Shuang
    Ruan, Shan
    Ma, Yanping
    Li, Mali
    Wang, Lin
    Zheng, Bo
    Qi, Ying
    Sun, Zhengrong
    Huang, Yujing
    Ruan, Qiang
    Powiązania:
    https://bibliotekanauki.pl/articles/1039141.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    HCMV
    UL4
    UL5
    transcript
    Opis:
    Human cytomegalovirus (HCMV) UL4 and UL5 genes are two members of the RL11 gene family. In an earlier study, three UL4 transcripts of about 1.7, 1.5 and 1.4 kb were found in early and late classes after infection by the Towne strain by nuclease protection and primer extension analyses. In the present study, two UL4 transcripts (1.5 and 1.7 kb) were found by cDNA library screening, Northern blot, 3' and 5' RACE analyses to appear initially in the immediate early phase and one UL4 transcript (1.4 kb) in the late phase in a low-passage clinical isolate. Furthermore, two novel low-abundance UL5 transcripts with the same 3' terminus as the identified UL4 transcripts in the UL4-UL5 gene region were found in late class RNAs.
    Źródło:
    Acta Biochimica Polonica; 2015, 62, 1; 97-101
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Diffraction mechanism of five-port transmission gratings
    Autorzy:
    Gao, Chenhao
    Wang, Bo
    Wen, Kunhua
    Meng, Ziming
    Wang, Qu
    Nie, Zhaogang
    Xing, Xiangjun
    Chen, Li
    Lei, Liang
    Zhou, Jinyun
    Powiązania:
    https://bibliotekanauki.pl/articles/1835817.pdf
    Data publikacji:
    2020
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    1 × 5 splitting
    single-groove grating
    normal incidence
    Opis:
    This paper designs a five-port transmission grating under normal incidence. Rigorous coupled-wave approach is used to optimize the grating parameters. The energy of the grating is mainly dispersed to the 0th, ±1st and ±2nd orders. The efficiency of each diffraction order under both polarizations is close to 20%. The modal method is used to describe the propagation mechanism of the two polarized lights in the grating, and the diffraction behavior of the grating is analyzed. In addition, the grating has a wide range of incident characteristics and a large process tolerance. Therefore, this five-port beam splitter with a connecting layer will be a good polarization-independent beam splitting device.
    Źródło:
    Optica Applicata; 2020, 50, 4; 609-618
    0078-5466
    1899-7015
    Pojawia się w:
    Optica Applicata
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Generation of three-port splitter by double-layer grating in second-order Littrow configuration
    Autorzy:
    Gao, Chenhao
    Wang, Bo
    Fu, Chen
    Fang, Jimin
    Wen, Kunhua
    Meng, Ziming
    Nie, Zhaogang
    Xing, Xiangjun
    Chen, Li
    Lei, Liang
    Zhou, Jinyun
    Powiązania:
    https://bibliotekanauki.pl/articles/174662.pdf
    Data publikacji:
    2020
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    three-port splitter
    second-order Littrow
    double-layer grating
    Opis:
    In this paper, a novel double-layer three-port grating is described. The incident grating structure is in the second-order Littrow configuration. The grating region is composed of fused silica and Ta2O5.The designed grating beam splitter has high efficiency under TE polarization and TM polarization, respectively. The efficiency of two polarizations is more than 90%. In addition, compared with a single-layer three-port grating, this new beam splitter has good fabrication tolerance and incident bandwidth. Therefore, the optimized structure has a good application value.
    Źródło:
    Optica Applicata; 2020, 50, 2; 271-280
    0078-5466
    1899-7015
    Pojawia się w:
    Optica Applicata
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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