Autor: Gal`chinsky, N. - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: A breakthrough in the efficiency of contact DNA insecticides: rapid high mortality rates in the sap-sucking insects Dynaspidiotus britannicus Comstock and Unaspis euonymi Newstead
Autorzy:: Gal`chinsky, N.
Useinov, R.
Yatskova, E.
Laikova, K.
Novikov, I.
Gorlov, M.
Trikoz, N.
Sharmagiy, A.
Plugatar, Y.
Oberemok, V.
Powiązania:: https://bibliotekanauki.pl/articles/2082776.pdf
Data publikacji:: 2020
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: antisense oligonucleotides
DNA insecticides
insect pest control
sap-sucking
insects
28S ribosomal RNA
Opis:: In this short communication describing experiments carried out on the larvae of two insects, Unaspis euonymi Comstock (feeding on Euonymus japonicus Thunb.) and Dynaspidiotus britannicus Newstead (feeding on Laurus nobilis L.), we evaluate for the first time the efficiency of using DNA insecticides in the control of sap-sucking insects, including armored scale insects. Over a period of 10 days, high insect mortality was detected in both U. euonymi and D. britannicus, accompanied by a significant decrease in the concentration of target RNAs. At the same time, no visible changes were observed when the leaves of the host plants were subjected to treatment with DNA insecticides for one month. The results show the high efficiency of DNA insecticides used against hemipteran insect pests. It is noteworthy that the high efficiency of DNA insecticides and their low cost in comparison with RNA preparations provides a safe and extremely promising potential vehicle for the control of sap-sucking insects.
Źródło:: Journal of Plant Protection Research; 2020, 60, 2; 220-223
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: A primary attempt of Leptinotarsa decemlineata control using contact DNA insecticide based on short antisense oligonucleotide of its CYP6B gene
Autorzy:: Oberemok, V.
Laikova, K.
Shumskykh, M.
Kenyo, I.
Kasich, I.
Deri, K.
Seidosmanova, E.
Krasnodubets, A.
Bekirova, V.
Gal'chinsky, N.
Powiązania:: https://bibliotekanauki.pl/articles/66808.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: Leptinotarsa decemlineata
pest control
DNA insecticide
insecticide
antisense oligonucleotide
cytochrome P450
CYP6B gene
potato
Opis:: Effective control of Leptinotarsa decemlineata remains an urgent problem for agriculture worldwide. Minimization of the use of non-selective neonicotinoid insecticides, such as thiomethoxam, is an actual vector of development of potato cultivation. In this rapid communication, we show the prospect of the topical use of short unmodified antisense fragment of L. decemlineata CYP6B gene as a DNA insecticide. Investigated parameters, namely, number of larvae per plant, aboveground biomass, yield and number of potatoes produced per plant indicate the possibility of this post-genomic approach as a safe and effective method of L. decemlineata control.
Źródło:: Journal of Plant Protection Research; 2018, 58, 1
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Insecticidal activity of three 10–12 nucleotides long antisense sequences from 5.8S ribosomal RNA gene of gypsy moth Lymantria dispar L. against its larvae
Autorzy:: Oberemok, V.V.
Laikova, K.V.
Useinov, R.Z.
Gal`chinsky, N.V.
Novikov, I.A.
Yurchenko, K.A.
Volkov, M.E.
Gorlov, M.V.
Brailko, V.A.
Plugatar
Powiązania:: https://bibliotekanauki.pl/articles/2084849.pdf
Data publikacji:: 2019
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: antisense oligonucleotides
DNA insecticides
gypsy moth
insect pest control
Lymantria dispar
5.8S ribosomal RNA
Opis:: 5.8S ribosomal RNA plays an important role in protein synthesis and eukaryotic ribosome translocation. Contact DNA insecticides based on antisense fragments of 5.8S ribosomal RNA gene of gypsy moth Lymantria dispar L. showed prospective insecticidal activity on its larvae. The most pronounced insecticidal effect was found for antisense fragments 10 and 11 nucleotides long (oligoRIBO-10 and oligoRIBO-11), whereas 12 nucleotides long fragment (oligoRIBO-12) caused the lowest level of insect mortality. This data corresponds to results obtained earlier using rabbit reticulocyte and wheat germ extracts, where maximum inhibition of protein synthesis was observed when a relevant oligomer 10-11 nucleotides long was used, whilst longer chain lengths resulted in reduced inhibition. Using oligoRIBO-11 fragment we have shown penetration of antisense oligonucleotides to insect cells through insects’ exoskeletons. MALDI technique registered the penetration of the oligoRIBO-11 fragment into insect cells after 30 min and a significant response of insect cells to the applied oligonucleotide after 60 min, which indicates not only that the oligonucleotide enters the insect cells, but also the synthesis of new substances in response to the applied DNA fragment. Contact DNA insecticides developed from the L. dispar 5.8S ribosomal RNA gene provide a novel biotechnology for plant protection using unmodified antisense oligonucleotides.
Źródło:: Journal of Plant Protection Research; 2019, 59, 4; 561-564
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: The need for the application of modern chemical insecticides and environmental consequences of their use: a mini review
Autorzy:: Oberemok, V.V.
Laikova, K.V.
Zaitsev, A.S.
Temirova, Z.Z.
Gal'chinsky, N.V.
Nyadar, P.M.
Shumskykh, M.N.
Zubarev, I.V.
Powiązania:: https://bibliotekanauki.pl/articles/66108.pdf
Data publikacji:: 2017
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: application need
modern insecticide
insecticide
chemical insecticide
environmental consequence
DNA insecticide
insect pest
insecticide resistance
plant protection
Opis:: Currently, the use of insecticides is an acute problem. Due to rapid population growth, the primary task is to increase food production. Beyond abiotic factors (drought, soil salinity, etc.) that reduce crop yields, farmers face problems with insect pests that can decrease crop productivity up to 60%. Also, insects are carriers of severe viral and protozoan human diseases. The need for application of insecticides is not questioned but many of them cause resistance of insect pests to them. This, in turn, leads to the necessity to invent new insecticides that are safe and more effective for long-term use. Preparations based on conservative parts of nucleic acids, particularly contact DNA insecticides, could be used to solve insecticide resistance problem as control agents which are well-tailored to target insect pests. This mini review is devoted to these issues.
Źródło:: Journal of Plant Protection Research; 2017, 57, 4
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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