- Tytuł:
- How the RNA isolation method can affect microRNA microarray results
- Autorzy:
-
Podolska, Agnieszka
Kaczkowski, Bogumil
Litman, Thomas
Fredholm, Merete
Cirera, Susanna - Powiązania:
- https://bibliotekanauki.pl/articles/1039846.pdf
- Data publikacji:
- 2011
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
microarray
microRNA isolation method
microRNA
small RNA fraction
pig - Opis:
- The quality of RNA is crucial in gene expression experiments. RNA degradation interferes in the measurement of gene expression, and in this context, microRNA quantification can lead to an incorrect estimation. In the present study, two different RNA isolation methods were used to perform microRNA microarray analysis on porcine brain tissue. One method is a phenol-guanidine isothiocyanate-based procedure that permits isolation of total RNA. The second method, miRVana™ microRNA isolation, is column based and recovers the small RNA fraction alone. We found that microarray analyses give different results that depend on the RNA fraction used, in particular because some microRNAs appear very sensitive to the RNA isolation method. We conclude that precautions need to be taken when comparing microarray studies based on RNA isolated with different methods.
- Źródło:
-
Acta Biochimica Polonica; 2011, 58, 4; 535-540
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł