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Wyświetlanie 1-7 z 7
Tytuł:
Microbial functional diversity and enzymatic activity of soil degraded by sulphur mining reclaimed with various waste
Autorzy:
Joniec, Jolanta
Frąc, Magdalena
Powiązania:
https://bibliotekanauki.pl/articles/972819.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
functional diversity
β-glucosidase activity
degraded soil
recultivation
waste
Opis:
The aim of the study was to evaluate microbial functional diversity based on community level physiological profiling and b-glucosidase activity changes in soil degraded by sulphur mining and subjected to reclamation with various waste. The experiment was set up in the area of the former ‘Jeziórko’ Sulphur Mine (Poland), on a soilless substrate with a particle size distribution of slightly loamy sand. The experimental variants included the application of post-flotation lime, sewage sludge and mineral wool. The analyses of soil samples included the assessment of the following microbiological indices: b-glucosidase activity and functional diversity average well color development and richness). The results indicate that sewage sludge did not exert a significant impact on the functional diversity of microorganisms present in the reclaimed soil. In turn, the application of other types of waste contributed to a significant increase in the parameters of total metabolic activity and functional diversity of the reclaimed soil. However, the temporal analysis of the metabolic profile of soil microorganisms demonstrated that a single application of waste did not yield a durable, stable metabolic profile in the reclaimed soil. Still, there was an increase in β-glucosidase activity, especially in objects treated with sewage sludge.
Źródło:
International Agrophysics; 2017, 31, 4; 465-473
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Determination of biodiversity of Coprinus comatus using genotyping and metabolic profiling tools
Autorzy:
Pawlik, Anna
Malinowska, Anna
Siwulski, Marek
Frąc, Magdalena
Rogalski, Jerzy
Janusz, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1038885.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
AFLP
ITS
Biolog
fungal diversity
Coprinus comatus
shaggy mane mushroom
Opis:
Coprinus comatus strains (CCMs) originating from Poland were identified using ITS region sequencing. Based on the sequences obtained, the genetic relationship between the CCM strains was determined and a clear separation of all strains into two main clusters was obtained. The Coprinus strains were also genetically characterized for the first time by the AFLP technique. The analysis showed that the CCMs separated into four main clusters and a high complication of a UPGMA-based dendrogram was achieved. C. comatus strains included in the analysis displayed an AFLP profile similarity level in the range from 44 to 66%. The highest similarity coefficient, 0.490, was found between CCM12 and CCM13, and the lowest (0.202) between the CCM2 and CCM5 isolates. Biolog FF MicroPlates were applied to obtain data on utilization of 95 carbon sources and mycelial growth. The analysis allowed comparison of the functional diversity of the CCM strains and revealed a broad variability within the analyzed Coprinus species based on substrate utilization profiles. Significant differences (2-48) have been shown in the substrate richness values. The Biolog experiments proved to be a good profiling technology for studying the diversity in shaggy manes due to metabolic differences and demonstrated that all the strains might be considered individually. It is evident that the strain metabolic grouping does not correlate with the grouping based on the ITS sequences and AFLP profiles, however, some similarities may be observed.
Źródło:
Acta Biochimica Polonica; 2015, 62, 4; 683-689
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Methanogenic community composition in an organic waste mixture in an anaerobic bioreactor
Autorzy:
Gryta, Agata
Oszust, Karolina
Brzezińska, Małgorzata
Ziemiński, Krzysztof
Bilińska-Wielgus, Nina
Frąc, Magdalena
Powiązania:
https://bibliotekanauki.pl/articles/973033.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
anaerobic digestion
methanogenic activity
methanogenic archaea
ngs
t-rflp
Opis:
The aim of the study was to elucidate the substantial relationship between the compositions of methanogen community that assembles in the anaerobic digester mass and link it to methane production activity. The results of the metagenomic studies were used to evaluate how the methanogen structure changes during an anaerobic digestion process under various waste retention times (21, 23, 25, 29, 33, 39, 47 and 61 days). Phylogenetically coherent populations of methanogens were assessed by 16S rRNA gene next-generation sequencing and terminal restriction fragment length polymorphism fingerprinting of a specific molecular marker, the mcrA gene. The results indicated multiple phylogenetically diverse methanogen populations associated with the various steps of anaerobic digestion. The stages of the anaerobic digestion process and waste retention times determine the microbial composition. The most dominant and acclimated microbial communities in all samples belonged to the genera Methanosaeta and Methanobacterium. The methane yield was consistent with the results of the microbial community structure, which indicated that acetotrophic Methanosaeta was the most active and most important during the methanogenic stage.
Źródło:
International Agrophysics; 2017, 31, 3
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The phenotypic and genomic diversity of Aspergillus strains producing glucose dehydrogenase
Autorzy:
Rola, Beata
Pawlik, Anna
Frąc, Magdalena
Małek, Wanda
Targoński, Zdzisław
Rogalski, Jerzy
Janusz, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1038906.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Aspergillus
glucose dehydrogenase
identification
metabolic diversity
Opis:
Twelve Aspergillus sp. strains producing glucose dehydrogenase were identified using ITS region sequencing. Based on the sequences obtained, the genomic relationship of the analyzed strains was investigated. Moreover, partial gdh gene sequences were determined and aligned. The amplified fragment length polymorphism (AFLP) method was applied for genomic fingerprinting of twelve Aspergillus isolates. Using one PstI restriction endonuclease and five selective primers in an AFLP assay, 556 DNA fragments were generated, including 532 polymorphic bands. The AFLP profiles were found to be highly specific for each strain and they unambiguously distinguished twelve Aspergilli fungi. The AFLP-based dendrogram generated by the UPGMA method grouped all the Aspergillus fungi studied into two major clusters. All the Aspergillus strains were also characterized using Biolog FF MicroPlates to obtain data on C-substrate utilization and mitochondrial activity. The ability to decompose various substrates differed among the analyzed strains up to three folds. All of the studied strains mainly decomposed carbohydrates.
Źródło:
Acta Biochimica Polonica; 2015, 62, 4; 747-755
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Community-level physiological profiles of microorganisms inhabiting soil contaminated with heavy metals
Autorzy:
Kuźniar, Agnieszka
Banach, Artur
Stępniewska, Zofia
Frąc, Magdalena
Oszust, Karolina
Gryta, Agata
Kłos, Marta
Wolińska, Agnieszka
Powiązania:
https://bibliotekanauki.pl/articles/973240.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Opis:
The aim of the study was to assess the diffe-rences in the bacterial community physiological profiles in soils contaminated with heavy metals versus soils without metal contaminations. The study’s contaminated soil originated from the surrounding area of the Szopienice non-ferrous metal smelter (Silesia Region, Poland). The control was soil unexposed to heavy metals. Metal concentration was appraised by flame atomic absorption spectrometry, whereas the the community-level physiological profi le was determined with the Biolog EcoPlatesTM system. The soil microbiological activity in both sites was also assessed via dehydrogenase activity. The mean concentrations of metals (Cd and Zn) in contaminated soil samples were in a range from 147.27 to 12265.42 mg kg-1, and the heavy metal contamination brought about a situation where dehydrogenase activity inhibition was observed mostly in the soil surface layers. Our results demonstrated that there is diversity in the physiological profi les of microorganisms inhabiting contaminated and colntrol soils; therefore, for assessment purposes, these were treated as two clusters. Cluster I included colntrol soil samples in which microbial communities utilised most of the available substrates. Cluster II incorporated contaminated soil samples in which a smaller number of the tested substrates was utilised by the contained microorganisms. The physiological profi les of microorganisms inhabiting the contaminated and the colntrol soils are distinctly different.
Źródło:
International Agrophysics; 2018, 32, 1; 101-109
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of microbially enriched mineral fertilizers on the composition of rhizosphere microorganisms of Thuja occidentalis
Autorzy:
Sas Paszt, Lidia
Smolińska, Urszula
Treder, Jadwiga
Szczech, Magdalena
Kowalska, Beata
Trzciński, Paweł
Głuszek, Sławomir
Lisek, Anna
Derkowska, Edyta
Frąc, Mateusz
Powiązania:
https://bibliotekanauki.pl/articles/2148193.pdf
Data publikacji:
2021-12-30
Wydawca:
Instytut Uprawy Nawożenia i Gleboznawstwa – Państwowy Instytut Badawczy
Tematy:
Thuja occidentalis cv. Brabant
rhizosphere
microorganisms
biofertilizers
Opis:
Rhizosphere is a region of the strongest interactions be- tween plants, soil and microorganisms, which play an important role in plant development. Due to a number of interdependencies, they are main factors determining the health and proper growth of plants. All methods used in agriculture that promote the growth of microbial populations and their biodiversity are beneficial both for cultivated plants and for the environment. Thuja occidentalis cv. Brabant, is a very popular plant in Poland. In the study the effect of mineral fertilizers: Urea, Polifoska 6, Super Fos Dar 40, enriched with beneficial strains of fungi and bacteria, on selected groups of soil microorganisms was evaluated. The fungi Asper- gillus niger and Purpureocillium lilacinum and bacteria Bacillus sp., Bacillus amyloliquefaciens, Paenibacillus polymyxa, which have been shown to have beneficial effects on plant growth in previous works, have been used as additives to bio-fertilizers. The use of mineral fertilizers Urea, Polifoska 6 and Super Fos Dar 40, enriched with selected strains of fungi and bacteria had a beneficial effect on the development of microorganisms in the rhizosphere of the Thuja occidentalis, especially in the second year of research.
Źródło:
Polish Journal of Agronomy; 2021, 47; 49-57
2081-2787
Pojawia się w:
Polish Journal of Agronomy
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Fizjologiczne i genetyczne markery odporności pszenicy jarej na suszę w aspekcie interakcji zachodzących w ryzosferze
Physiological and genetic markers of resistance of spring wheat to drought in the aspect of interactions occurring in the rhizosphere
Autorzy:
Szechyńska-Hebda, Magdalena
Hordyńska, Natalia
Grzesiak, Stanisław
Grzesiak, Maciej
Sas-Paszt, Lidia
Lisek, Anna
Sumorok, Beata
Derkowska, Edyta
Weszczak, Krzysztof
Frąc, Mateusz
Przybył, Michał
Trzciński, Paweł
Głuszek, Sławomir
Powiązania:
https://bibliotekanauki.pl/articles/2199475.pdf
Data publikacji:
2019-11-30
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
mikroorganizmy pożyteczne
pszenica
ryzosfera
susza
Źródło:
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin; 2019, 286; 47-48
0373-7837
2657-8913
Pojawia się w:
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-7 z 7

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