Autor: Fiedor, Leszek - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Antioxidant effects of carotenoids in a model pigment-protein complex
Autorzy:: Fiedor, Joanna
Sulikowska, Aleksandra
Orzechowska, Aleksandra
Fiedor, Leszek
Burda, Květoslava
Powiązania:: https://bibliotekanauki.pl/articles/1039774.pdf
Data publikacji:: 2012
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: carotenoid
antioxidant
stability
chemical oxidation
Opis:: The effect of carotenoids on stability of model photosynthetic pigment-protein complexes subjected to chemical oxidation with hydrogen peroxide or potassium ferricyanide was investigated. The oxidation of carotenoid-less and carotenoid-containing complexes was conducted in the presence or absence of ascorbic acid. The progress of the reactions was monitored by use of absorption and fluorescence spectroscopy. Our results show that carotenoids may significantly enhance the stability of photosynthetic complexes against oxidation and their protective (antioxidant) effect depends on the type of the oxidant.
Źródło:: Acta Biochimica Polonica; 2012, 59, 1; 61-64
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Optimization of Western blotting analysis for the isolation and detection of membrane xenobiotic transporter ABCG2
Autorzy:: Szczygieł, Małgorzata
Markiewicz, Marcin
Szafraniec, Milena
Zuziak, Roxana
Urbańska, Krystyna
Fiedor, Leszek
Powiązania:: https://bibliotekanauki.pl/articles/1038582.pdf
Data publikacji:: 2017
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: Xenobiotic transporter
ABCG2
BCRP
Western blotting
Opis:: All organisms are exposed to numerous stress factors, which include harmful xenobiotics. The diversity of these compounds is enormous, thus in the course of evolution diverse biological defense mechanisms at various levels of organization have developed. One of them engages an evolutionarily conserved family of transporters from the ABC superfamily, found in most species - from bacteria to humans. An important example of such a transporter is the breast cancer resistance protein (BCRP/ABCG2), a typical integral membrane protein. It plays a key role in the absorption, distribution and elimination of a wide variety of xenobiotics, including drugs used in chemotherapy, and is involved in multidrug resistance. It also protects against phototoxic chlorophyll derivatives of dietary origin. BCRP is a hemitransporter which consists of one transmembrane domain, made of six alpha-helices forming a characteristic pore structure, and one ATP-binding domain, which provides the energy from ATP hydrolysis, required for active transport of the substrates. The isolation of BCRP is still not an easy task, because its insolubility in water and the presence of membrane rafts pose serious methodological and technical challenges during the purification. The aim of this study was to optimize the methods for detection and isolation of BCRP-enriched fractions obtained from animal tissue samples. In this report we describe an optimization of isolation of a BCRP-enriched membrane fraction, which is suitable for further protein quantitative and qualitative analysis using the molecular biology tools.
Źródło:: Acta Biochimica Polonica; 2017, 64, 3; 437-443
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Pulmonary metastases of the A549-derived lung adenocarcinoma tumors growing in nude mice. A multiple case study
Autorzy:: Jakubowska, Monika
Śniegocka, Martyna
Podgórska, Ewa
Michalczyk-Wetula, Dominika
Urbanska, Krystyna
Susz, Anna
Fiedor, Leszek
Pyka, Janusz
Płonka, Przemysław
Powiązania:: https://bibliotekanauki.pl/articles/1039526.pdf
Data publikacji:: 2013
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: adenocarcinoma
histological analysis
metastases
nude mice
photodynamic therapy
zinc pheophorbide a
Opis:: Lung adenocarcinoma is a leading human malignancy with fatal prognosis. Ninety percent of the deaths, however, are caused by metastases. The model of subcutaneous tumor xenograft in nude mice was adopted to study the growth of control and photodynamically treated tumors derived from the human A549 lung adenocarcinoma cell line. As a side-result of the primary studies, observations on the metastasis of these tumors to the murine lungs were collected, and reported in the present paper. The metastasizing primary tumors were drained by a prominent number of lymphatic vessels. The metastatic tissue revealed the morphology of well-differentiated or trans-differentiated adenocarcinoma. Further histological and histochemical analyses demonstrated the presence of golden-brown granules in the metastatic tissue, similar to these found in the tumor tissue. In contrast to the primary tumors, the electron paramagnetic resonance spectroscopy revealed no nitric oxide - hemoglobin complexes (a source of intense paramagnetic signals), in the metastases. No metastases were found in other murine organs; however, white infarctions were identified in a single liver. Taken together, the A549-derived tumors growing subcutaneously in nude mice can metastasize and grow on site in the pulmonary tissue. Thus, they can represent an alternative for the model of induced metastatic nodule formation, following intravenous administration of the cancerous cells.
Źródło:: Acta Biochimica Polonica; 2013, 60, 3; 323-330
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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