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Wyszukujesz frazę "El-Zanaty, A.M." wg kryterium: Autor


Wyświetlanie 1-2 z 2
Tytuł:
Screening and identification of novel cellulolytic Trichoderma species from Egyptian habitats
Autorzy:
Hewedy, O.A.
El-Zanaty, A.M.
Fahmi, A.I.
Powiązania:
https://bibliotekanauki.pl/articles/2097005.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Trichoderma
cellulase activity
biodegradation
ITS
tef1
Opis:
The present study aimed to isolate indigenous Trichoderma fungi possessing cellulolytic activities from different geographical locations in Egypt. Such strains can be used to convert crop residues to bioproducts, which presently is one of the most important issues in developing countries. The isolates were identified using molecular approaches and morphological characteristics. Favorable growth conditions for Trichoderma isolates (media & temperature) were assessed in vitro. The growth rate of Trichoderma isolates was assessed on two media, namely Potato Dextrose Agar (PDA) and Synthetic Nutrient Agar (SNA), and morphological parameters, i.e., shape, pigment, and edge were observed. A comparison of sequences encoding for internal transcribed spacer (ITS) and translation elongation factor 1-α (tef1 ) was conducted to determine the species. Cellulase assay was performed using different cellulose substrates (Swollen, CMC (low viscosity carboxymethylcellulose), and Avicel). Among the isolated fungi, Trichoderma viride EGY-T27 had the highest endoglucanase activity, while Trichoderma harzianum EGY-T30 showed the highest exoglucanase activity. A total of 27 strains of Trichoderma were identified using ITS, and the tef1 gene was sequenced for 23 strains. The tested strains were identified with acceptable quality based on ITS sequences, which ranged from 98 to 100%, except for strain MH908499 (85%). This research constitutes the most comprehensive study on screening for fungal strains with the highest biodegradable potential isolated from various locations in Egypt.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 2; 117-133
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic characterization of some rhizobial isolates from various legumes
Autorzy:
El-Ghany, E.M.A.
Eissa, R.A.
Fahmi, A.I.
Nagaty, H.H.
El-Zanaty, A.M.
Powiązania:
https://bibliotekanauki.pl/articles/2097126.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
rhizobia
morphological
API 20E and API 20NE
16S rRNA
phylogenetic
Opis:
In the last few decades, there has been a growing interest in environmentally friendly sustainable agricultural practices, thus increasing the role of biofertilizers such as rhizobia, which can decrease the need for chemical fertilizers, reduce adverse environmental effects, and help to save money. Therefore, information on the distribution and genetic variation of native rhizobial isolates would aid in selecting novel rhizobial strains that could be developed and used as biofertilizers in legume production. This research was conducted to characterize 24 rhizobial isolates from five legumes on morphological, biochemical, and molecular aspects and determine the phylogenetic relationships among them. Rhizobial isolates were obtained from five Egyptian legumes: faba bean, lentil, pea, clover, and soybean. Morphological characterization classified the isolates into fast and slow growers. Biochemical characterization using API 20E and API 20NE systems showed a large diversity, which may reflect their adaptation in different environments. Moreover, molecular detection of the 16S rRNA gene enabled to characterize 19 of them to the species level. Rhizobial isolates from pea, faba bean, clover, and lentil were identified as Rhizobium leguminosarum and those from soybean were identified as Bradyrhizobium japonicum. These data reflected a narrow diversity of rhizobial species in Egypt. A phylogenetic analysis of the 19 isolates confirmed that B. japonicum isolates were divergent from all other isolates. Furthermore, the phylogram revealed that each group of isolates that originated from the root nodule of a certain legume formed a separate subcluster. The obtained data suggested a narrow range of interspecies variations, which is consistent with the idea of the presence of biovars among the species.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 3; 179-191
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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