Autor: Dziadek, Marta - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Mycobacterium tuberculosis - the 10 years of epidemiological and diagnostics studies
Autorzy:: Boruń-Popławska, Marta
Sajduda, Anna
Brzostek, Anna
Dziadek, Jarosław
Powiązania:: https://bibliotekanauki.pl/articles/764949.pdf
Data publikacji:: 2005
Wydawca:: Uniwersytet Łódzki. Wydawnictwo Uniwersytetu Łódzkiego
Tematy:: Mycobacterium tuberculosis
insertion sequences
PCR
epidemiology
diagnosis
Opis:: Tuberculosis (ТВ) is the main bacterial pathogen that causes more deaths than AIDS, malaria and all infectious diseases. The unusual long doubling time (about 24h), highly hydrophobic cell envelope resistant to chemical lysis was the reason to delay the molecular study of this bacteria. Fifteen years ago, we did not have any molecular tools and methods for genetic manipulation or isolation and analysis of intracellular protein and nucleic acids. Today we have many useful shuttle or integration vectors for basic study of mycobacteria. The full sequence of M. tuberculosis genome is already known. At the present time the diagnosis of tuberculosis is supported with fast-culture system BACTEC and molecular techniques based on PCR and DNA hybridization. The mechanisms of resistance to antituberculosis drugs were described, and first identification of resistance profile is available by using PCR and sequencing or real time PCR methods. In our group in the Center for Microbiology and Virology Polish Academy of Sciences and in the Dept, of Genetics of Microorganisms, University Łódź we have characterized new insertion sequences from M. tuberculosis complex- 18990 and IS1607. In diagnostic studies we have proposed the DIG-PCR ELISA assay as a reliable, specific and sensitive test to identify M. tuberculosis directly in clinical samples. We have performed wide epidemiological studies of M. tuberculosis strains isolated from Polish ТВ patients including drug - and multidrug - resistant strains. Finally we identified the most frequently present mutations responsible for drug resistance of polish clinical isolates of M. tuberculosis.
Źródło:: Acta Universitatis Lodziensis. Folia Biologica et Oecologica; 2005, 2
1730-2366
2083-8484
Pojawia się w:: Acta Universitatis Lodziensis. Folia Biologica et Oecologica
Dostawca treści:: Biblioteka Nauki

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Tytuł:: Salivary aldehyde dehydrogenase - temporal and population variability, correlations with drinking and smoking habits and activity towards aldehydes contained in food
Autorzy:: Giebułtowicz, Joanna
Dziadek, Marta
Wroczyński, Piotr
Woźnicka, Katarzyna
Wojno, Barbara
Pietrzak, Monika
Wierzchowski, Jacek
Powiązania:: https://bibliotekanauki.pl/articles/1040385.pdf
Data publikacji:: 2010
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: superoxide dismutase
fluorescence
aldehyde dehydrogenase
aldehydes
nutrition safety
saliva
salivary peroxidase
Opis:: Fluorimetric method based on oxidation of the fluorogenic 6-methoxy-2-naphthaldehyde was applied to evaluate temporal and population variability of the specific activity of salivary aldehyde dehydrogenase (ALDH) and the degree of its inactivation in healthy human population. Analyzed was also its dependence on drinking and smoking habits, coffee consumption, and its sensitivity to N-acetylcysteine. Both the specific activity of salivary ALDH and the degree of its inactivation were highly variable during the day, with the highest activities recorded in the morning hours. The activities were also highly variable both intra- and interpersonally, and negatively correlated with age, and this correlation was stronger for the subgroup of volunteers declaring abstinence from alcohol and tobacco. Moderately positive correlations of salivary ALDH specific activity with alcohol consumption and tobacco smoking were also recorded (rs ~0.27; p=0.004 and rs =0.30; p=0.001, respectively). Moderate coffee consumption correlated positively with the inactivation of salivary ALDH, particularly in the subgroup of non-drinking and non-smoking volunteers. It was found that mechanical stimulation of the saliva flow increases the specific activity of salivary ALDH. The specific activity of the salivary ALDH was strongly and positively correlated with that of superoxide dismutase, and somewhat less with salivary peroxidase. The antioxidant-containing drug N-acetylcysteine increased activity of salivary ALDH presumably by preventing its inactivation in the oral cavity. Some food-related aldehydes, mainly cinnamic aldehyde and anisaldehyde, were excellent substrates of the salivary ALDH3A1 enzyme, while alkenals, particularly those with short chain, were characterized by lower affinity towards this enzyme but high catalytic constants. The protective role of salivary ALDH against aldehydes in food and those found in the cigarette smoke is discussed, as well as its participation in diminishing the effects of alcohol- and smoking-related oxidative stress.
Źródło:: Acta Biochimica Polonica; 2010, 57, 3; 361-368
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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