Autor: Dworecka-Kaszak, Bożena - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: The use of a one-step PCR method for the identification of Microsporum canis and Trichophyton mentagrophytes infection of pets
Autorzy:: Dąbrowska, Iwona
Dworecka-Kaszak, Bożena
Brillowska-Dąbrowska, Anna
Powiązania:: https://bibliotekanauki.pl/articles/1039306.pdf
Data publikacji:: 2014
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: dermatophytosis
animals
pan-dermatophytes
PCR
Opis:: Introduction: Dermatophytes are a closely related group of keratinophilic fungi. They encompass important etiological agents of superficial fungal infections. These fungi are able to invade keratinized tissues of humans and animals, causing dermatophytosis (ringworm) of hair, nails or skin. The aim: Traditional diagnostics of ringworm is based on morphological identification of cultured fungi and is time-consuming. Materials and methods: In this study, we applied a method patented by Brillowska-Dabrowska and coworkers (Brillowska-Dąbrowska A, Saunte DM, Arenderup MC, 2007, Five-hour diagnosis of dermatophyte nail infections with specific detection of Trichophyton rubrum. J Clin Microbiol 45: 1200-1204) which involves extraction of fungal DNA and PCR amplification with pan-dermatophyte primers to confirm the presence of dermatophytes. Results: The method used here is able to confirm the presence of dermatophyte DNA in pure cultures in less than 5 hours.
Źródło:: Acta Biochimica Polonica; 2014, 61, 2; 375-378
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: PCR and real-time PCR assays to detect fungi of Alternaria alternata species
Autorzy:: Kordalewska, Milena
Brillowska-Dąbrowska, Anna
Jagielski, Tomasz
Dworecka-Kaszak, Bożena
Powiązania:: https://bibliotekanauki.pl/articles/1038891.pdf
Data publikacji:: 2015
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: Alternaria alternata
detection
identification
PCR
real-time PCR
Opis:: Fungi of the Alternaria genus are mostly associated with allergic diseases. However, with a growing number of immunocompromised patients, these fungi, with A. alternata being the most prevalent one, are increasingly recognized as etiological agents of infections (phaeohyphomycoses) in humans. Nowadays, identification of Alternaria spp. requires their pure culture and is solely based on morphological criteria. Clinically, Alternaria infections may be indistinguishable from other fungal diseases. Therefore, a diagnostic result is often delayed or even not achieved at all. In this paper we present easy to perform and interpret PCR and real-time PCR assays enabling detection of A. alternata species. On the basis of alignment of β-tubulin gene sequences, A. alternata-specific primers were designed. DNA from fungal isolates, extracted in a two-step procedure, were used in PCR and real-time PCR assays followed by electrophoresis or melting temperature analysis, respectively. The assays specificity was confirmed, since positive results were obtained for all A. alternata isolates, and no positive results were obtained neither for other molds, dermatophytes, yeast-like fungi, nor human DNA. The assays developed here enable fast and unambiguous identification of A. alternata pathogens.
Źródło:: Acta Biochimica Polonica; 2015, 62, 4; 707-712
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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