- Tytuł:
- The influence of PPM upon the sterility of the in vitro cultures in European beech [Fagus sylvatica L.]
- Autorzy:
-
Kraj, W
Dolnicki, A. - Powiązania:
- https://bibliotekanauki.pl/articles/56550.pdf
- Data publikacji:
- 2003
- Wydawca:
- Polskie Towarzystwo Botaniczne
- Tematy:
-
Plant Preservative Mixture
plant physiology
tissue culture
tree bud
Fagus sylvatica
sterilization
beech
sterility
in vitro
embryo
European beech
bud - Opis:
- Investigations were conducted on the strongly infested by microbes embryos isolated from seed stratified in non-sterile conditions or from tree buds. The results showed, that PPM (Plant Preservative Mixture, produced by Plant Cell Technology, Inc.) was very useful for increasing the percentage of sterile cultures disinfected in a 5% solution of NaOCl. The best way of applying PPM turned out to be adding the substance directly to the growing medium. At the concentration of 2 or 4 cm3 x dm-3 of PPM there was an 30% increase in percentage of sterile of in vitro cultures developed from embryos and 70-80% of cultures developed from buds, as compared with the control without PPM. At the range of concentrations employed in this study, no negative effects of PPM upon the development and growth of callus were observed.
- Źródło:
-
Acta Societatis Botanicorum Poloniae; 2003, 72, 4
0001-6977
2083-9480 - Pojawia się w:
- Acta Societatis Botanicorum Poloniae
- Dostawca treści:
- Biblioteka Nauki
Artykuł