- Tytuł:
- Initial Phases of DNA Rehydration by NMR and Sorption Isotherm
- Autorzy:
-
Harańczyk, H.
Czak, J.
Nowak, P.
Nizioł, J. - Powiązania:
- https://bibliotekanauki.pl/articles/1539094.pdf
- Data publikacji:
- 2010-02
- Wydawca:
- Polska Akademia Nauk. Instytut Fizyki PAN
- Tematy:
- 82.56.Na
- Opis:
- T*he initial stages of rehydration of salmon sperm deoxyribonucleic acid (DNA) lyophilizates were observed using hydration kinetics, sorption isotherm, and high power proton relaxometry (at 30 MHz). T*he hydration kinetics reveals (i) a very tightly bound water not removed by incubation over silica gel ($A_0^{h}$ = 0.057 ± 0.010), (ii) a tightly bound water [saturating at $A_1^{h}$ = 0.149 ± 0.007, hydration time $t_1^{h}$ = (0.27 ± 0.08) h], a tightly bound water (iii) [saturating at $A_2^{h}$ = 0.694 ± 0.039, with the hydration time $t_2^{h}$ = (9.8 ± 3.2) h], and (iv) a loosely bound water fraction for the samples hydrated at p/$p_{0}$ ≥ 76% [with the hydration time $t_3^{h}$ = (44 ± 14) h, and the contribution progressively increasing with the air humidity]. For the hydration at p/$p_{0}$ = 100%, after $t_{0}$ = (244 ± 22) h of incubation the swelling process begins. T*he amount of additional water uptake at swelling depended on the macrostructure of the sample. Sorption isotherm is sigmoidal in form and is fitted well by the Dent model with the mass of water saturating primary binding sites Δ M/$m_{0}$ = 0.114. Proton free induction decay is a superposition of the immobilized proton signal (Gaussian, with $T_{2S}^\ast$ ≈ 20 μs) and two liquid signal components coming from tightly bound ($T_{2L_1}^\ast$ ≈ 100 μs, with the mass saturating at Δ m/$m_{0}$ = 0.111 ± 0.044) and loosely bound water fraction (with the amplitude proportional to the mass of water added).
- Źródło:
-
Acta Physica Polonica A; 2010, 117, 2; 397-402
0587-4246
1898-794X - Pojawia się w:
- Acta Physica Polonica A
- Dostawca treści:
- Biblioteka Nauki
Artykuł