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Wyszukujesz frazę "Cisak, J." wg kryterium: Autor


Wyświetlanie 1-32 z 32
Tytuł:
Estimation of height changes of GNSS stations from the solutions of short vectors and PSI measurements
Autorzy:
Krynski, J.
Zak, L.
Ziolkowski, D.
Cisak, J.
Lagiewska, M.
Powiązania:
https://bibliotekanauki.pl/articles/145302.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
height changes
GNSS
Persistent Scatterer Interferometry
radar satelitarny
deformacja powierzchni
Opis:
Time series of weekly and daily solutions for coordinates of permanent GNSS stations may indicate local deformations in Earth’s crust or local seasonal changes in the atmosphere and hydrosphere. The errors of the determined changes are relatively large, frequently at the level of the signal. Satellite radar interferometry and especially Persistent Scatterer Interferometry (PSI) is a method of a very high accuracy. Its weakness is a relative nature of measurements as well as accumulation of errors which may occur in the case of PSI processing of large areas. It is thus beneficial to confront the results of PSI measurements with those from other techniques, such as GNSS and precise levelling. PSI and GNSS results were jointly processed recreating the history of surface deformation of the area of Warsaw metropolitan with the use of radar images from Envisat and Cosmo-SkyMed satellites. GNSS data from Borowa Gora and Jozefoslaw observatories as well as from WAT1 and CBKA permanent GNSS stations were used to validate the obtained results. Observations from 2000–2015 were processed with the Bernese v.5.0 software. Relative height changes between the GNSS stations were determined from GNSS data and relative height changes between the persistent scatterers located on the objects with GNSS stations were determined from the interferometric results. The consistency of results of the two methods was 3 to 4 times better than the theoretical accuracy of each. The joint use of both methods allows to extract a very small height change below the level of measurement error.
Źródło:
Geodesy and Cartography; 2017, 66, 1; 73-88
2080-6736
2300-2581
Pojawia się w:
Geodesy and Cartography
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena wystepowania kretkow Borrelia burgdorferi sensu lato w kleszczach Ixodes ricinus na terenie wybranych rejonow Lubelszczyzny przy zastosowaniu metody lancuchowej reakcji polimerazy [PCR]
Autorzy:
Chmielewska-Badora, J
Cisak, E.
Zwolinski, J.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/838855.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wystepowanie
kleszcze
parazytologia
Ixodes ricinus
lancuchowa reakcja polimerazy
Borrelia burgdorferi
kretki
Lubelszczyzna
metody oceny
Opis:
Evaluation of occurrence of spirochetes Borrelia burgdorferi sensu lato in Ixodes ricinus ticks in selected areas of the Lublin Region by polimerase chain reaction method (PCR). During the period 2001-2002, 1098 Ixodes ricinus ticks were collected at forest sampling sites and the degree of their infection with Borrelia burgdorferi spirochetes was determined by means of polimerase chain reaction (PCR). The presence of Borrelia burgdorferi genetic material was noted in 69 cases (6.3%). lt was confirmed that the frequency of infection of adult forms of ticks (males and females) was nearly twice as high as nymphs. The highest degree of infection was observed in females – 9.5%. The degree of infection among males and nymphs was smaller - 5.9% and 4.4% respectively in individual provinces. The percentage of infected females ranged from 7.9% in the Zamość Province to 13.6% in the Włodawa Province. In males, the percentage of infected ticks remained within the range from 3.1% in the Lublin Province to 13.3% in the Lubartów Province.
Źródło:
Annals of Parasitology; 2003, 49, 2; 165-171
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena narazenia na zakazenie wirusem kleszczowego zapalenia mozgu osob zawodowo eksponowanych na kontakt z kleszczami
Autorzy:
Cisak, E
Sroka, J.
Zwolinski, J.
Chmielewska-Badora, J.
Powiązania:
https://bibliotekanauki.pl/articles/836275.pdf
Data publikacji:
1999
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
zagrozenia zdrowia
choroby wirusowe
wektory wirusow
rolnicy
kleszcze
przenoszenie chorob
parazytologia
kleszczowe zapalenie mozgu
epidemiologia
choroby odzwierzece
lesnicy
Opis:
The paper presents the results of seroepidemiologic studies concerning tick-borne encephalitis (TBE) in 1583 persons (1261 forestry workers and 233 farmers) from the Lublin region (eastern Poland) occupationally exposed to ticks and in 130 healthy blond donors (a control group). The mean percentage of seropositive reactions in forestry workers amounted 19.8% and in farmers 32.0%. Based on 5-year research (1994-1998) conducted in 5 districts of the Lublin region, an existence of endemic foci of TBE was detected in the district of Biała Podlaska. on the areas of Radzyń Podlaski and Parczew, where the percentage of seropositive reactions in forestry workers exceeded 50%. Statistical analysis showed that the frequency of seropositive reactions in forestry workers and farmers was significantly greater compated to control group (p<0.001 and p<0.05, respectively). It indicates that these groups are occupationally exposed to TBE wirus. Totally, in the years 1994-98 nine clinical cases of TBE (acute neuroinfection) in forestry workers and fourteen clinical cases in farmers were confirmed serologically. The effectiveness of specific immunization againstTBE was proved on the brie of 100% seroconversion in 56 earlier seronegative forestry workers. The obtained results proved that forestry workers and farmers in Poland are under increased risk of infection with TBE virus.
Źródło:
Annals of Parasitology; 1999, 45, 3; 375-380
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena występowania krętków Borrelia burgdorferi sensu lato w kleszczach Ixodes ricinus na terenie wybranych rejonów Lubelszczyzny przy zastosowaniu metody łańcuchowej reakcji polimerazy [PCR]
Autorzy:
Chmielewska-Badora, J.
Cisak, E.
Zwoliński, J.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/2147621.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wystepowanie
kleszcze
parazytologia
Ixodes ricinus
lancuchowa reakcja polimerazy
Borrelia burgdorferi
kretki
Lubelszczyzna
metody oceny
Opis:
Evaluation of occurrence of spirochetes Borrelia burgdorferi sensu lato in Ixodes ricinus ticks in selected areas of the Lublin Region by polimerase chain reaction method (PCR). During the period 2001-2002, 1098 Ixodes ricinus ticks were collected at forest sampling sites and the degree of their infection with Borrelia burgdorferi spirochetes was determined by means of polimerase chain reaction (PCR). The presence of Borrelia burgdorferi genetic material was noted in 69 cases (6.3%). lt was confirmed that the frequency of infection of adult forms of ticks (males and females) was nearly twice as high as nymphs. The highest degree of infection was observed in females – 9.5%. The degree of infection among males and nymphs was smaller - 5.9% and 4.4% respectively in individual provinces. The percentage of infected females ranged from 7.9% in the Zamość Province to 13.6% in the Włodawa Province. In males, the percentage of infected ticks remained within the range from 3.1% in the Lublin Province to 13.3% in the Lubartów Province.
Źródło:
Wiadomości Parazytologiczne; 2003, 49, 2; 165-171
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Toxoplasma gondii antibodies in forest workers from Lublin Macroregion
Autorzy:
Uminski, J.
Cisak, E.
Badora, J.
Sroka, J.
Zwolinski, J.
Powiązania:
https://bibliotekanauki.pl/articles/840504.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Lublin macroregion
toxoplasmosis
zoonosis
Toxoplasma gondii
forest worker
antibody
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Risk of tick-borne encephalitis virus [TBE] infection among people occupationally exposed to tick bites
Autorzy:
Cisak, E.
Sroka, J.
Zwolinski, J.
Chmielewska-Badora, J.
Powiązania:
https://bibliotekanauki.pl/articles/839837.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
zoonosis
infection
man
tick-borne encephalitis virus
farmer
Lublin region
forest worker
tick
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena narażenia na zakażenie wirusem kleszczowego zapalenia mózgu osób zawodowo eksponowanych na kontakt z kleszczami
RISK OF TISK-BORNE ENCEPHALITIS (TBE) VIRUS INFECTION AMONG PEOPLE OCCUPATIONALLY EXPOSED TO TICK BITES.
Autorzy:
Cisak, E.
Sroka, J.
Zwoliński, J.
Chmielewska-Badora, J.
Powiązania:
https://bibliotekanauki.pl/articles/2148815.pdf
Data publikacji:
1999
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
zagrozenia zdrowia
choroby wirusowe
wektory wirusow
rolnicy
kleszcze
przenoszenie chorob
parazytologia
kleszczowe zapalenie mozgu
epidemiologia
choroby odzwierzece
lesnicy
Opis:
The paper presents the results of seroepidemiologic studies concerning tick-borne encephalitis (TBE) in 1583 persons (1261 forestry workers and 233 farmers) from the Lublin region (eastern Poland) occupationally exposed to ticks and in 130 healthy blond donors (a control group). The mean percentage of seropositive reactions in forestry workers amounted 19.8% and in farmers 32.0%. Based on 5-year research (1994-1998) conducted in 5 districts of the Lublin region, an existence of endemic foci of TBE was detected in the district of Biała Podlaska. on the areas of Radzyń Podlaski and Parczew, where the percentage of seropositive reactions in forestry workers exceeded 50%. Statistical analysis showed that the frequency of seropositive reactions in forestry workers and farmers was significantly greater compated to control group (p<0.001 and p<0.05, respectively). It indicates that these groups are occupationally exposed to TBE wirus. Totally, in the years 1994-98 nine clinical cases of TBE (acute neuroinfection) in forestry workers and fourteen clinical cases in farmers were confirmed serologically. The effectiveness of specific immunization againstTBE was proved on the brie of 100% seroconversion in 56 earlier seronegative forestry workers. The obtained results proved that forestry workers and farmers in Poland are under increased risk of infection with TBE virus.
Źródło:
Wiadomości Parazytologiczne; 1999, 45, 3; 375-380
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Toxoplasmosis in the course of sclerosis multiplex. Preliminary observation
Autorzy:
Uminski, J.
Cisak, E.
Chmielewska-Badora, J.
Zwolinski, J.
Sroka, J.
Powiązania:
https://bibliotekanauki.pl/articles/836463.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
toxoplasmosis
patient
serological test
immunofluorescence assay
multiple sclerosis
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Poszukiwanie ognisk toksoplazmozy rodzinnej w środowisku wiejskim
Detecting centres of family Toxoplasmosis in rural environment
Autorzy:
Umiński, J.
Cisak, E.
Chmielewska-Badora, J.
Skomra, S.
Powiązania:
https://bibliotekanauki.pl/articles/2152533.pdf
Data publikacji:
1989
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
parazytologia
choroby pasozytnicze
toksoplazmoza
ogniska chorobowe
srodowisko rodzinne
rodziny wiejskie
srodowisko wiejskie
badania serologiczne
Opis:
In order to detect centres of family toxoplasmosis in rural environment 24.546 patients, suspected of invasion of T. gondii, 504 healthy persons coming from villages and 1.681 live stock were examined. Among persons suspected of infection of T. gondii, 65 cases of congenital family toxoplasmosis and 19 cases of environment family toxoplasmosis, involving several members of the family were detected. In animals a comparatively high percentage with positive reaction in the direct agglutination test (cattle 55.5%, swine 27.90/o, sheep 23.4%) was detected. The following serological tests were applied to humans: complement-binding test (OWD), indirect immunofluorescence test (OIF), direct agglutination test with 2-mercaptoethanol (CA), ELISA IgM and, selectively, immunoperoxidase test IgM (OIP). The results suggest that the environment conditions can play an important role in the occurrence of family toxoplasmosis in our country, especially in the countryside. Detecting active toxoplasmosis in a family member should be a signal to start serological-epidemiological examination of the remaining family members and live stock.
Źródło:
Wiadomości Parazytologiczne; 1989, 35, 4; 289-297
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wystepowanie przeciwcial przeciw Anaplasma phagocytophilum i Borrelia burgdorferi u lesnikow w regionie lubelskim
Autorzy:
Zwolinski, J.
Chmielewska-Badora, J.
Cisak, E.
Buczek, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/841017.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wystepowanie
region lubelski
przeciwciala
Anaplasma phagocytophilum
parazytologia
Borrelia burgdorferi
pasozyty
lesnicy
Źródło:
Annals of Parasitology; 2004, 50, 2; 221-227
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Występowanie przeciwciał przeciw Anaplasma phagocytophilum i Borrelia burgdorferi u leśnikow w regionie lubelskim
Prevalence of antibodies to Anaplasma phagocytophilum and Borrelia burgdorferi in forestry workers from the Lublin region
Autorzy:
Zwoliński, J.
Chmielewska-Badora, J.
Cisak, E.
Buczek, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/2146456.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wystepowanie
region lubelski
przeciwciala
Anaplasma phagocytophilum
parazytologia
Borrelia burgdorferi
pasozyty
lesnicy
Opis:
Ticks are recently regarded as ones of the most important vectors of emerging diseases. One of these diseases is human granulocytic ehrlichiosis caused by obligatory intracellular microorganism recently named as Anaplasma phagocytophilum. 334 sera from forestry workers and 56 sera from blood donors (as a control group) were collected and examined for antibodies to A. phagocytophilum and B. burgdorferi. The antibodies were found statistically more frequently in sera from foresters compared to sera of the control group, both to A. phagocytophilum (19,8% vs. 5,4%, p < 0,001) and B. burgdorferi (32,0% vs. 7,1%, p < 0,01). Only 15 sera (4.5%) were found positive for both examined bacteria suggesting inhibition of coexistence although it was not statistically significant. No correlation was found between percentages of antibodies to A. phagocytophilum and to B. burgdorferi from different locations.
Źródło:
Wiadomości Parazytologiczne; 2004, 50, 2; 221-227
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Profilaktyka chorób odkleszczowych
Prevention of tick-borne diseases
Autorzy:
Dutkiewicz, J
Cisak, E.
Wójcik-Fatla, A.
Zając, V.
Sroka, J.
Powiązania:
https://bibliotekanauki.pl/articles/179873.pdf
Data publikacji:
2014
Wydawca:
Centralny Instytut Ochrony Pracy
Tematy:
choroby odkleszczowe
choroby zawodowe
patogeny
profilaktyka
tick-borne diseases
occupational
pathogens
prevention
Opis:
Chorobami odkleszczowymi nazywamy choroby zakaźne przenoszone za pośrednictwem drobnych pajęczaków, zwanych kleszczami (lxodida). W Polsce są one wywoływane przez co najmniej 5 gatunków (lub grup gatunków) mikroorganizmów (wirusów, bakterii, pierwotniaków). Spośród nich największe znaczenie ma krętek Borrelia burgdorferi wywołujący boreliozę z Lyme, który jest częstą przyczyną chorób zawodowych u leśników i rolników. Główne kierunki profilaktyki chorób odkleszczowych obejmują: szczepienia ochronne przeciwko kleszczowemu zapaleniu mózgu i opon mózgowo-rdzeniowych, unikanie spożywania surowego mleka, stosowanie odpowiedniej odzieży ochronnej podczas pracy w lesie lub na obrzeżach lasu, przegląd ciała i wzięcie natrysku po powrocie z pracy w lesie, szybkie i umiejętne usuwanie kleszczy przyssanych do ciała, stosowanie repelentów, edukację zdrowotną, opiekę medyczną nad zagrożonymi pracownikami leśnictwa i rolnictwa, działania prewencyjne ze strony pionu bhp lasów państwowych, redukcję liczebności kleszczy w środowisku naturalnym.
Tick-borne diseases are infectious diseases transmitted by small arachnids called ticks (lxodida). In Poland, these diseases are caused by at least five species (or groups of species) of microorganisms (viruses, bacteria. protozoa). Of which, the most important is the spirochete Borrelia burgdorferi causing Lyme borreliosis, which is the frequent cause of occupational diseases in the forestry and agricultural workers. The main directions of the prevention of tick-borne diseases include: vaccination against tick-borne encephalitis, avoiding of the consumption of raw milk, use of the proper clothing during work in the forest, inspection of the body and taking shower after coming from forest. quick and proper removing of ticks adhered to body, use of repelltents, health education, providing medical care for endangered forestry and agricultural workers, preventive actions pursued by the management of the forest service, reduction of the tick number of natural environment.
Źródło:
Bezpieczeństwo Pracy : nauka i praktyka; 2014, 4; 21-23
0137-7043
Pojawia się w:
Bezpieczeństwo Pracy : nauka i praktyka
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Risk of Lyme disease at various sites and workplaces of forestry workers in eastern Poland
Autorzy:
Cisak, E.
Wojcik-Fatla, A.
Zajac, V.
Sroka, J.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/52094.pdf
Data publikacji:
2012
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2012, 19, 3
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Risk of tick-borne diseases in various categories of employment among forestry workers in eastern Poland
Autorzy:
Cisak, E.
Zajac, V.
Wojcik-Fatla, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51019.pdf
Data publikacji:
2012
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2012, 19, 3
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Badania seroepidemiologiczne w kierunku Francisella tularensis wśród pracowników leśnictwa i rolników
Seroepidemiological study on Francisella tularensis in forestry workers and farmers
Autorzy:
Cisak, E.
Wojcik‑Fatla, A.
Zajac, V.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/4445.pdf
Data publikacji:
2014
Wydawca:
Instytut Medycyny Wsi
Źródło:
Medycyna Ogólna i Nauki o Zdrowiu; 2014, 20(49), 4
2083-4543
Pojawia się w:
Medycyna Ogólna i Nauki o Zdrowiu
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Repellents and acaricides as personal protection measures in the prevention of tick-borne diseases
Autorzy:
Cisak, E.
Wojcik-Fatla, A.
Zajac, V.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/50494.pdf
Data publikacji:
2012
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2012, 19, 4
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biological agents as occupational hazards - selected issues
Autorzy:
Dutkiewicz, J.
Cisak, E.
Sroka, J.
Wojcik-Fatla, A.
Zajac, V.
Powiązania:
https://bibliotekanauki.pl/articles/50867.pdf
Data publikacji:
2011
Wydawca:
Instytut Medycyny Wsi
Tematy:
biological agent
occupational hazard
health hazard
bioaerosol
allergy
human disease
toxin
virus
bacteria
fungi
infectious disease
zoonosis
tick-borne disease
Źródło:
Annals of Agricultural and Environmental Medicine; 2011, 18, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Profilaktyka chorób odzwierzęcych innych niż kleszczowe
Prevention of zoonotic diseases other than tick-borne
Autorzy:
Dutkiewicz, J
Wójcik-Fatla, A.
Zając, V.
Sroka, J.
Cisak, E.
Powiązania:
https://bibliotekanauki.pl/articles/179719.pdf
Data publikacji:
2014
Wydawca:
Centralny Instytut Ochrony Pracy
Tematy:
choroby odzwierzęce
patogeny
drogi przenoszenia
profilaktyka
zoonoses
occupational
pathogens
transmission routes
prevention
Opis:
W Polsce występuje co najmniej 31 ważnych patogenów (wirusy, bakterie, grzyby, pierwotniaki, robaki), wywołujących zawodowe choroby odzwierzęce inne niż odkleszczowe. Spośród tych patogenów 9 przekazywanych jest głównie drogą powietrzną, 9 - drogą pokarmową i 13 - drogą kontaktu bezpośredniego przez skórę i/lub błony śluzowe. Najważniejsze kierunki profilaktyki chorób odzwierzęcych obejmują: utrzymywanie czystości w pomieszczeniach dla zwierząt i dokonywanie okresowych dezynfekcji, stosowanie odzieży ochronnej i sprzętu ochrony osobistej, profilaktykę medyczną, wybijanie zagrożonych stad, tępienie gryzoni, szczepienia ochronne ludzi i zwierząt, zachowanie szczególnej ostrożności przy odbieraniu porodów u przeżuwaczy, przestrzeganie zasad higieny przy spożywaniu pokarmów i wody i edukację zdrowotną.
In Poland, there are at least 31 important pathogens (viruses, bacteria, fungi, protozoa, helminths) causing occupational zoonotic diseases other than tick-borne. Of that number, 9 are transmitted mainly by airborne route, 9 by alimentary route and 13 by direct contact through the skin and/or mucous membranes.The main directions using protective clothing and equipment, medical prophylaxis, eradicating endangered flocks, combating rodents, vaccinating humans and animals, taking precautions at parturitions of ruminants, maintaining hygiene at food and water consumption, and health education.
Źródło:
Bezpieczeństwo Pracy : nauka i praktyka; 2014, 6; 12-14
0137-7043
Pojawia się w:
Bezpieczeństwo Pracy : nauka i praktyka
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence of Anaplasma phagocytophilum in Ixodes ricinus ticks determined by polymerase chain reaction with two pairs of primers detecting 16S rRNA and ankA genes
Autorzy:
Chmielewska-Badora, J
Zwolinski, J.
Cisak, E.
Wojcik-Fatla, A.
Buczek, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51023.pdf
Data publikacji:
2007
Wydawca:
Instytut Medycyny Wsi
Tematy:
Lublin region
Polska
pathogen
Ixodes ricinus
Eastern Poland
tick
determination
animal pathogen
human pathogen
ankA gene
16S rRNA gene
granulocytic anaplasmosis
bacteria
Anaplasma phagocytophilum
polymerase chain reaction
Opis:
A total of 684 Ixodes ricinus ticks (321 nymphs, 184 males, and 179 females) were collected by fl agging lower vegetation in 6 forest districts located on the territory of Lublin province (eastern Poland). Ticks were examined by polymerase chain reaction (PCR) method for the presence of Anaplasma phagocytophilum DNA with two pairs of primers: EHR521/EHR747 for detecting 16S rRNA gene, and LA6/LA1 for detecting ankA gene. To study the relationship between infection in ticks and people occupationally exposed to tick bite, blood serum samples of 261 forestry workers employed in the same forest districts were examined by immunofl uorescence method for the presence of specifi c antibodies against A. phagocytophilum. A total of 70 ticks out of 684 examined (10.2%) showed the presence of A. phagocytophilum 16S rRNA gene. The prevalence of infection was signifi cantly dependent on tick’s stage (χ2=49.2, p<0.00001) and geographical locality (χ2=34.4, p<0.00001). The percentage of I. ricinus females infected with A. phagocytophilum (24.6%) was signifi cantly greater compared to males (6.5%) and nymphs (4.4%) (p<0.00001). Only 19 ticks out of 684 examined (2.8%) showed the presence of A. phagocytophilum ankA gene, signifi cantly less compared to 16S rRNA gene (p<0.00001). The prevalence of infection demonstrated by the presence of ankA gene was also signifi cantly dependent on tick’s stage (χ2=23.6, p<0.00001) but not on locality (χ2=9.8, p=0.082). A signifi cant correlation was found between the presence of A. phagocytophilum 16S rRNA gene in I. ricinus female ticks from the particular forest districts and the serologic response to A. phagocytophilum of forestry workers employed in the same districts (p<0.05). No signifi cant correlation was found between the presence of A. phagocytophilum ankA gene in I. ricinus ticks and serologic response of exposed workers. In conclusion, detection of A. phagocytophilum infection in ticks by PCR with the use of EHR521/EHR747 primers detecting 16S rRNA gene is signifi cantly more sensitive compared to LA6/LA1 primers detecting ankA gene.
Źródło:
Annals of Agricultural and Environmental Medicine; 2007, 14, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence of tick-borne encephalitis virus [TBEV] in samples of raw milk taken randomly from cows, goats and sheep in Eastern Poland
Autorzy:
Cisak, E
Wojcik-Fatla, A.
Zajac, V.
Sroka, J.
Buczek, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/50068.pdf
Data publikacji:
2010
Wydawca:
Instytut Medycyny Wsi
Tematy:
tick-borne encephalitis virus
prevalence
raw milk
milk
milk sample
cow
goat
sheep
Polska
epidemiology
antibody
Źródło:
Annals of Agricultural and Environmental Medicine; 2010, 17, 2; 283-286
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study on tick-borne rickettsiae in eastern Poland. I. Prevalence in Dermacentor reticulatus (Acari: Amblyommidae)
Autorzy:
Wojcik-Fatla, A.
Cisak, E.
Zajac, V.
Sroka, J.
Sawczyn, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/50634.pdf
Data publikacji:
2013
Wydawca:
Instytut Medycyny Wsi
Tematy:
Rickettsia
Rickettsia raoultii
Polska
prevalence
Dermacentor reticulatus
tick
Acari
Amblyommidae
Opis:
Rickettsia spp. transmitted by ticks are classified mostly in the Spotted Fever Group Rickettsiae (SFGR). Numerous species of this group have been identified in Eurasia as emerging pathogens, but still little is known about their occurrence, effects on human health, and co-incidence with other tick-borne pathogens. The aim of the presented study was to determine the prevalence of Rickettsia spp. in adult Dermacentor reticulatus (Acari: Amblyommidae) ticks collected in Lublin province of eastern Poland using the PCR method. The infection rate of D. reticulatus with Rickettsia spp. was 53.0%. All except one rickettsial isolates showed 100% homology with Rickettsia raoultii. A high prevalence of R. raoultii in Dermacentor reticulatus ticks from eastern Poland suggests that the SFGR species should be considered as potential causative agents of tick-borne diseases in this area.
Źródło:
Annals of Agricultural and Environmental Medicine; 2013, 20, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Leptospirosis as a tick-borne disease? Detection of Leptospira spp. in Ixodes ricinus ticks in eastern Poland
Autorzy:
Wojcik-Fatla, A.
Zajac, V.
Cisak, E.
Sroka, J.
Sawczyn, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51864.pdf
Data publikacji:
2012
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2012, 19, 4
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A role of the heat and work uncompensated transformations in the balance of entropy and the turbomachinery efficienc
Autorzy:
Kowalczyk, T.
Ziółkowski, P.
Sławiński, D.
Cisak, M.
Badur, J.
Powiązania:
https://bibliotekanauki.pl/articles/175450.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
entropy balance
entropy sources
uncompensated transformations
isentropic efficiency
polytropic efficiency
eulerian fluid
turbine stage
Puzyrewski number
Opis:
The notion of heat uncompensated transformation has early been introduced by Clausius in 1854 and next, after fifty years of forgetting, in 1904 Duhem has revalorized it and combined it with a new notion of work uncompensated transformation [3]. In this way the so-called ClausiusDuhem inequality has been established. In our paper we wish to present a novelized procedure of estimating the role of the uncompensated transformations of heat and work within the flow of viscous and heat conducting working fluid like water stream. The original procedure was introduced by Puzyrewski and it is essential in expressing of a local, in time and space, balance of entropy. Futhermore, this unique approach leads to redefinition of the efficiency notion, as is usually applied to fluid-flow machineries, to a new one important in computational fluid dynamics (CFD) three-dimensional modeling. As a result, it is shown that usage of the polytropic efficiency, instead of the isentropic efficiency, is more convenient and seems to be natural in CFD approach. Helpfully, we have also found a correlation between those two efficiency definitions with usage of proposed new dimensionless (criterion) number.
Źródło:
Transactions of the Institute of Fluid-Flow Machinery; 2017, 135; 11-27
0079-3205
Pojawia się w:
Transactions of the Institute of Fluid-Flow Machinery
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study on tick-borne rickettsiae in eastern Poland. II. Serological response of occupationally exposed populations
Autorzy:
Zajac, V.
Wojcik-Fatla, A.
Cisak, E.
Sroka, J.
Sawczyn, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51448.pdf
Data publikacji:
2013
Wydawca:
Instytut Medycyny Wsi
Tematy:
tick
Rickettsia
Polska
serological response
occupational exposure
population
serology
ELISA test
forestry worker
agricultural worker
Opis:
A group of 150 persons living in the Lublin province of eastern Poland and occupationally exposed to tick bite were examined by the immunoenzymatic ELISA test for the presence of antibodies against tick-borne Spotted Fever Group (SFG) rickettsiae. The group consisted of 75 forestry workers employed in 3 forest inspectorates and 75 agricultural workers living in 2 villages. As a control group, 43 urban dwellers living in the city of Lublin and not occupationally exposed to tick bite were examined. Among 150 persons occupationally exposed to tick bite, the presence of antibodies against SFG rickettsiae was found in 54 (36.0% of the total). In the control group, the frequency of positive findings was only 4.7%, being significantly smaller compared to the exposed group (p=0.0001). Within the exposed group, the percentage of positive results in forestry workers (50.7%) was greater than in agricultural workers (21.3%); the difference was statistically significant (p=0.0002). Also within this group, the frequency of positive findings in males(46.5%) was significantly greater than in females (21.9%) (p=0.0029). In the exposed group, the positive results tended to increase with the age of the examined persons. However, a significant relationship between age and positive findings was found only in forestry workers (c2=14.207, p=0.00264), but not in agricultural workers and total exposed workers. The frequencies of positive results in forestry workers varied significantly depending on place of work (c2=11.271, p=0.00357). Similarly, the difference between the positive reactions in agricultural workers living in 2 villages proved to be significant (34.2% vs. 8.1%; p=0.0074). The obtained results indicate that people occupationally exposed to tick bite and living in the area of eastern Poland where over half of Dermacentor reticulatus ticks harbour SFG rickettsiae, are under significantly increased risk of infection with these rickettsiae.
Źródło:
Annals of Agricultural and Environmental Medicine; 2013, 20, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A small scale survey of Leptospira in mammals from eastern Poland
Autorzy:
Wojcik-Fatla, A.
Zajac, V.
Sroka, J.
Piskorski, M.
Cisak, E.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51664.pdf
Data publikacji:
2013
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2013, 20, 4
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study on Lyme borreliosis focus in the Lublin region [eastern Poland]
Autorzy:
Cisak, E
Chmielewska-Badora, J.
Zwolinski, J.
Wojcik-Fatla, A.
Zajac, V.
Skorska, C.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51535.pdf
Data publikacji:
2008
Wydawca:
Instytut Medycyny Wsi
Tematy:
borreliosis
Polska
rural inhabitant
Lyme borreliosis
tick
seroepidemiology
Ixodes ricinus
Borrelia burgdorferi
clinical case
Lublin region
Źródło:
Annals of Agricultural and Environmental Medicine; 2008, 15, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A small-scale survey of hantavirus in mammals from eastern Poland
Autorzy:
Wojcik-Fatla, A.
Zajac, V.
Knap, J.P.
Sroka, J.
Cisak, E.
Sawczyn, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51577.pdf
Data publikacji:
2013
Wydawca:
Instytut Medycyny Wsi
Tematy:
hantavirus
Bunyaviridae
mammal
small mammal
Microtus agrestis
Myodes glareolus
Sorex araneus
epidemiology
polymerase chain reaction
flood
Polska
Opis:
Samples of 30 dead small mammals each were collected on area ‘A’ located in eastern Poland which is exposed to flooding by the Vistula river, and on the area ‘B’, also located in eastern Poland but not exposed to flooding. Kidneys and livers of the mammals were examined by the PCR and nested PCR methods for the presence of hantavirus RNA. Out of 7 species of small mammals examined, the presence of hantaviruses was detected in 4 of them. Hantavirus prevalence was low in Apodemus agrarius (2.6%), the most numerous mammal species, whereas in the remaining 3 positive species (Microtus agrestis, Myodes glareolus, Sorex araneus) this was 12.5–100%. The presence of hantaviruses was detected only in the animals found on area ‘A’ exposed to flooding, and their prevalence was statistically greater compared to area ‘B’ not exposed to flooding (16.7% vs. 0%, p=0.0345). The overall positivity of the examined small mammals population from the areas ‘A’ and ‘B’ was 8.3%. The sequence analysis of the samples positive for hantavirus proved that the amplified products showed 77–86% homology with the L segment sequence of hantavirus Fusong-Mf-731 isolated from Microtus fortis in China. The presented study is the first to demonstrate the occurrence of hantavirus infection in small mammals from eastern Poland, and the first to demonstrate the significant relationship between flooding and the prevalence of hantaviruses in small mammals.
Źródło:
Annals of Agricultural and Environmental Medicine; 2013, 20, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Occurrence of Leptospira DNA in water and soil samples collected in eastern Poland
Autorzy:
Wojcik-Fatla, A.
Zajac, V.
Wasinski, B.
Sroka, J.
Cisak, E.
Sawczyn, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51299.pdf
Data publikacji:
2014
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2014, 21, 4
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seroprevalence of leptospirosis in rural populations inhabiting areas exposed andnot exposed to floods in eastern Poland
Autorzy:
Wasinski, B.
Sroka, J.
Wojcik-Fatla, A.
Zajac, V.
Cisak, E.
Knap, J.P.
Sawczyn, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/50328.pdf
Data publikacji:
2012
Wydawca:
Instytut Medycyny Wsi
Opis:
Blood serum samples collected from randomly selected groups of 100 persons inhabiting rural community ‘A’ located in eastern Poland and exposed to floods by the Vistula river, and 98 persons inhabiting rural community ‘B’, also located in eastern Poland, but in the area not exposed to floods were examined by the microscopic agglutination test (MAT) for the presence of antibodies against 18 Leptospira serovars. Positive results showed 3% of serum samples collected from community ‘A’, while the seroprevalence in community ‘B’ was 9.2%, being insignificantly higher than in community ‘A’. For both examined communities (n=198), a significant positive correlation was found between the prevalence and titer of seropositive response and age of examined people (r=0.145, p=0.042). No significant differences were found between the prevalence of positive reactions in males and females (p>0.05). The reactions with 10 serovars of Leptospira (Australis, Autumnalis, Hebdomadis, Hardjo, Sejroe, Zanoni, Bataviae, Bratislava, Canicola and Grippotyphosa), belonging to 3 species (L. interrogans, L. borgpetersenii, L. kirschneri), were found in the examined communities. From both communities, of 12 persons demonstrating positive results in MAT, 9 showed reaction with one serovar, 2 with two serovars and 1 with three serovars. The highest titers found during the examination did not exceeded 800. In conclusion, our results suggest that there is only a slight, if any, hazard of an leptospirosis epidemic after the flood that afflicted eastern Poland in the year 2010 and the general epidemiological situation of leptospirosis in eastern Poland. Although there does not seem to be any cause for concern, further research is needed.
Źródło:
Annals of Agricultural and Environmental Medicine; 2012, 19, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena narażenia populacji wiejskiej Lubelszczyzny na zakażenie krętkami z rodzaju Leptospira, ze szczególnym uwzględnieniem terenów popowodziowych
Assessment of risk of infection with Leptospira spirochetes among rural population in the Lublin Region, with particular consideration of areas exposed to flooding
Autorzy:
Dutkiewicz, J.
Wojcik-Fatla, A.
Zajac, V.
Wasinski, B.
Knap, P.J.
Sroka, J.
Cisak, E.
Sawczyn, A.
Powiązania:
https://bibliotekanauki.pl/articles/4449.pdf
Data publikacji:
2015
Wydawca:
Instytut Medycyny Wsi
Opis:
Wprowadzenie. Leptospiroza, wywoływana przez krętki z rodzaju Leptospira, jest uważana za najbardziej rozprzestrzenioną chorobę odzwierzęcą na świecie. Zapadalność może być zwiększona przez klęski żywiołowe takie jak powodzie i tajfuny. Cel pracy. Celem pracy było zbadanie sytuacji epidemiologicznej leptospirozy na Lubelszczyźnie (wschodnia Polska), z uwzględnieniem wpływu powodzi Wisły, poprzez badania populacji ludzkiej i różnych elementów środowiska na obecność Leptospira w dwóch rejonach: rejonie „A” nawiedzanym przez powodzie i rejonie „B” nienawiedzanym przez powodzie. Materiał i metody. W rejonach „A” i „B” przebadano surowice, odpowiednio 100 i 98 mieszkańców, surowice 32 i 41 świń oraz surowice 41 i 40 krów – na obecność przeciwciał anty- Leptospira, wykorzystując test aglutynacji mikroskopowej (MAT). We wskazanych rejonach przebadano również, odpowiednio 40 i 64 próbki wody, 40 i 68 próbek gleby, próbki organów 30 i 30 drobnych ssaków należących do 5 i 6 gatunków, a także 540 i 296 kleszczy Ixodes ricinus – na obecność DNA Leptospira; badanie przeprowadzono testem nested-PCR. Wyniki. Obecność przeciwciał anty-Leptospira stwierdzono u 3% mieszkańców rejonu „A” i u 9,2% mieszkańców rejonu „B”; różnica ta nie była statystycznie znamienna. Częstość występowania przeciwciał anty-Leptospira była większa u świń i krów z rejonu „A” w porównaniu z rejonem „B” (odpowiednio 34,4% wobec 4,9%, i 26,8% wobec 15,0%), a w przypadku świń różnica okazała się znamienna (P=0,0015). Także miana dodatnich reakcji były wyższe w rejonie „A” w porównaniu z „B”, a w przypadku krów różnica okazała się znamienna (P=0,0128). Obecność DNA Leptospira spp. stwierdzono u 20% drobnych ssaków z rejonu „A” i u 30% z rejonu „B”; różnica ta nie była znamienna, chociaż częstość dodatnich wyników była w obu przypadkach wysoka w porównaniu z danymi z piśmiennictwa. Przeważającą część wyników dodatnich uzyskano u myszy polnych (Apodemus agrarius). Obecność DNA Leptospira spp. wykryto u 15,6% kleszczy I. ricinus z rejonu „A” w porównaniu z 1,4% w rejonie „B”, a różnica ta okazała się wysoce znamienna (P<0,0001). DNA Leptospira spp. zawierało 5% próbek wody z rejonu „A”, podczas gdy wszystkie próbki wody z rejonu „B” oraz wszystkie próbki gleby z rejonów „A” i „B” były ujemne. Wnioski. • Zakażenia krętkami Leptospira występujące u ludności wiejskiej zamieszkującej tereny Lubelszczyzny nawiedzane przez powodzie Wisły nie są częstsze w porównaniu z ludnością zamieszkującą inne części regionu nienawiedzane przez powodzie. • Potencjalnym źródłem zakażeń leptospirami na terenach nawiedzanych przez powodzie są zwierzęta: kleszcze Ixodes ricinus, zwierzęta hodowlane (świnie, krowy) i myszy polne (Apodemus agrarius). • Wykrycie DNA Leptospira spp. w 5% próbek wody z terenów popowodziowych, przy braku pozytywnych wyników z terenu kontrolnego, wydaje się potwierdzać możliwą rolę wody w szerzeniu leptospirozy. • Gleba nie stanowi istotnego zagrożenia na terenach nawiedzanych przez powodzie.
Background. Leptospirosis, caused by Leptospira spirochetes, is considered the most widespread zoonosis worldwide. Morbidity may be increased by natural disasters such as floods or typhoons. Aim of the study. The aim of the present study was to investigate the epidemiological situation of leptospirosis in the Lublin Region (Eastern Poland) with consideration of the effects of flooding by the Vistula River, by investigating human population and various elements of the environment for the presence of Leptospira in two areas: ‘Area A’ exposed to flooding, and ‘Area B’ not exposed to flooding. Material and Methods. In the Areas A and B, sera of 100 and 98 inhabitants, sera of 32 and 41 pigs and sera of 41 and 40 cows were examined, respectively, for the presence of anti-Leptospira antibodies by the microscopic agglutination test (MAT), as well as 40 and 64 samples of water, 40 and 68 samples of soil, organ samples of 30 and 30 small mammals from 5 and 6 species, and 540 and 296 Ixodes ricinus ticks, respectively – for the presence of Leptospira DNA by the nested-PCR test. Results. The presence of anti-Leptospira antibodies was found in 3% of inhabitants of Area A and in 9.2% of Area B; this difference was statistically insignificant. The frequency of anti-Leptospira antibodies was higher in pigs and cows from Area A, compared to area B (34.4% vs. 4.9%, and 26.8% vs. 15.0%, respectively), while in the case of pigs the difference was significant (P=0.0015). Also, the titers of positive reactions were higher in Area A, compared to Area B, and for cows the difference was significant (P=0.0128). The presence of Leptospira DNA was found in 20% of small mammals from Area A, and in 30% from Area B; this difference being insignificant; however, in both cases the frequency of positive results was high, compared to the data from literature. The great majority of positive results were obtained in striped field mice (Apodemus agrarius). The presence of Leptospira DNA was detected in 15.6% of I. ricinus ticks from Area A, compared to 1.4% in Area B, and the difference was highly significant (P<0.0001). 5% of water samples from Area A contained Leptospira DNA, whereas all water samples from Area B, and all soil samples from Areas A and B were negative. Conclusions. • The infections with Leptospira spirochetes among the rural population inhabiting the areas of the Lublin Region exposed to floods by the Vistula River do not occur with a higher frequency, compared to the areas not exposed to floods. • The potential sources of infection with Leptospira in the areas exposed to flooding are animals: ticks Ixodes ricinus, domestic animals (pigs, cows), and striped field mice (Apodemus agrarius). • Detection of Leptospira spp. DNA in 5% of water samples in the areas exposed to floods, with negative results in control areas, seems to confirm a possible role of water in spreading leptospirosis. • Soil does not constitute any significant risk of infection in the areas exposed to flooding.
Źródło:
Medycyna Ogólna i Nauki o Zdrowiu; 2015, 21(50), 1
2083-4543
Pojawia się w:
Medycyna Ogólna i Nauki o Zdrowiu
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Streptococcus suis: a re-emerging pathogen associated with occupational exposure to pigs or pork products. Part II – pathogenesis
Autorzy:
Dutkiewicz, J.
Zając, V.
Sroka, J.
Wasiński, B.
Cisak, E.
Sawczyn, A.
Kloc, A.
Wojcik-Fatla, A.
Powiązania:
https://bibliotekanauki.pl/articles/2081593.pdf
Data publikacji:
2018
Wydawca:
Instytut Medycyny Wsi
Tematy:
Streptococcus suis
pathogenesis
infection stages
interaction with host cells and tissues
virulence factors
genomic
determinants of pathogenicity
biofilm formation
Opis:
Abstract Streptococcus suis is a re-emerging zoonotic pathogen that may cause severe disease, mostly meningitis, in pigs and in humans having occupational contact with pigs and pork, such as farmers, slaughterhose workers and butchers. The first stage of the pathogenic process, similar in pigs and humans, is adherence to and colonisation of mucosal and/or epithelial surface(s) of the host. The second stage is invasion into deeper tissue and extracellular translocation of bacterium in the bloodstream, either free in circulation or attached to the surface of monocytes. If S. suis present in blood fails to cause fatal septicaemia, it is able to progress into the third stage comprising penetration into host’s organs, mostly by crossing the blood-brain barrier and/or blood–cerebrospinal fluid barrier to gain access to the central nervous system (CNS) and cause meningitis. The fourth stage is inflammation that plays a key role in the pathogenesis of both systemic and CNS infections caused by S. suis. The pathogen may induce the overproduction of pro-inflammatory cytokines that cause septic shock and/or the recruitment and activation of different leukocyte populations, causing acute inflammation of the CNS. Streptococcus suis can also evoke – through activation of microglial cells, astrocytes and possibly other cell types – a fulminant inflammatory reaction of the brain which leads to intracranial complications, including brain oedema, increased intracranial pressure, cerebrovascular insults, and deafness, as a result of cochlear sepsis. In all stages of the pathogenic process, S. suis interacts with many types of immunocompetent host’s cells, such as polymorphonuclear leukocytes, mononuclear macrophages, lymphocytes, dendritic cells and microglia, using a range of versatile virulence factors for evasion of the innate and adaptive immune defence of the host, and for overcoming environmental stress. It is estimated that S. suis produces more than 100 different virulence factors that could be classified into 4 groups: surface components or secreted elements, enzymes, transcription factors or regulatory systems and transporter factors or secretion systems. A major virulence factor is capsular polysaccharide (CPS) that protects bacteria from phagocytosis. However, it hampers adhesion to and invasion of host’s cells, release of inflammatory cytokines and formation of the resistant biofilm which, in many cases, is vital for the persistence of bacteria. It has been demonstrated that the arising by mutation unencapsulated S. suis clones, which are more successful in penetration to and propagation within the host’s cells, may coexist in the organism of a single host together with those that are encapsulated. Both ‘complementary’ clones assist each other in the successful colonization of host’s tissues and persistence therein. S. suis has an open pan-genome characterized by a frequent gene transfer and a large diversity. Of the genetic determinants of S. suis pathogenicity, the most important are pathogenicity islands (PAI), in particular, a novel DNA segment of 89 kb length with evident pathogenic traits that has been designated as 89K PAI. It has been estimated that more than one-third of the S. suis virulence factors is associated with this PAI. It has been proved that the virulent S. suis strains possess smaller genomes, compared to avirulent ones, but more genes associated with virulence. Overall, the evolution of the species most probably aims towards increased pathogenicity, and hence the most significant task of the current research is an elaboration of a vaccine, efficient both for humans and pigs.
Źródło:
Annals of Agricultural and Environmental Medicine; 2018, 25, 1; 186-203
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-32 z 32

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