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    Wyświetlanie 1-7 z 7
    Tytuł:
    The factor VIII protein and its function
    Autorzy:
    Mazurkiewicz-Pisarek, Anna
    Płucienniczak, Grażyna
    Ciach, Tomasz
    Płucienniczak, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/1038835.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Factor VIII (FVIII)
    molecular structure
    function
    hemophilia A
    bleeding disorders
    Opis:
    Factor VIII (FVIII), an essential blood coagulation protein, is a key component of the fluid phase blood coagulation system. Human factor VIII is a single chain of about 300 kDa consisting of domains described as A1-A2-B-A3-C1-C2. The protein undergoes processing prior to secretion into blood resulting in a heavy chain of 200 kDa (A1-A2-B) and a light chain of 80 kDa (A3-C1-C2) linked by metal ions. The role of factor VIII is to increase the catalytic efficiency of factor IXa in the activation of factor X. Variants of these factors lead frequently also to severe bleeding disorders.
    Źródło:
    Acta Biochimica Polonica; 2016, 63, 1; 11-16
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Solution blow spun poly-L-lactic acid/ceramic fibrous composites for bone implant applications
    Autorzy:
    Wojasiński, Michał
    Ciach, Tomasz
    Powiązania:
    https://bibliotekanauki.pl/articles/2086798.pdf
    Data publikacji:
    2021
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    solution blow spinning
    composite fibres
    submicron fibres
    nanofibres
    poly-L-lactic acid
    ceramic particles
    bone implants
    wirowanie z rozdmuchiwaniem roztworu
    włókna kompozytowe
    włókna submikronowe
    nanowłókna
    kwas poli-L-mlekowy
    cząstki ceramiczne
    implanty kostne
    Opis:
    Every bone implant to work correctly after implantation needs to integrate with the surrounding bone. To enhance such a process, called osseointegration, various techniques of implant surface modification emerged. One of the approaches is based on the deposition of nano- and submicron materials on the implant surface. This paper presents a solution blow spinning process for producing poly-L-lactic acid (PLLA)/ceramic fibrous composites designed to be deposited directly onto orthopaedic implants prior to implantation to increase osseointegration. We produced plain PLLA fibrous materials for comparison, and fibrous composite materials with -tricalcium phosphate (TCP), hydroxyapatite nanoparticles (nHAp) and hydroxyapatite nanoparticles modified with lecithin (nHAp-LE). We performed the structural analysis of produced materials with scanning electron microscopy, gravimetric determination of porosity, and water contact angle measurement. We also used infrared spectroscopy, Alizarin Red S staining, and cytotoxicity evaluation to conclude that PLLA/nHAp-LE composite material shows the most promising properties to be applied as surface modification of bone implants. To visualise fibrous composite deposition on implants, we used two models: titanium plate and stainlesssteel bolt. Thus, we showed that the solution blow spun materials can be used for surface modification of orthopaedic implants.
    Źródło:
    Chemical and Process Engineering; 2021, 42, 3; 275--289
    0208-6425
    2300-1925
    Pojawia się w:
    Chemical and Process Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Hydrogel microspheres containing dextran-based nanoparticles as novel anticancer drug delivery system
    Autorzy:
    Kulikowska-Darłak, Aleksandra
    Stefanek, Agata
    Wasiak-Wojasińska, Iga
    Wiechecka-Ożdżyńska, Paulina
    Ciach, Tomasz
    Powiązania:
    https://bibliotekanauki.pl/articles/2202877.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    alginate
    dextran
    nanoparticles
    microspheres
    drug delivery
    alginian
    dekstran
    nanocząstki
    mikrosfery
    dostarczanie leków
    Opis:
    Developing an effective and safe cancer therapy could significantly reduce the number of deaths and improve the quality of life of treated patients. Nowadays medicine has developed a wide range of anticancer chemotherapeutics but at the same time there is a lack of effective drug delivery methods. Therefore, the development of the targeted drug delivery system which will selectively release drug into the cancer cells is a key challenge of modern medicine. The main aim of the presented research was to investigate the targeting effect of a drug delivery system based on the controlled release of dextran nanoparticles containing the anticancer drug – doxorubicin from the alginate microspheres coated with chitosan multilayers. During the research the physicochemical properties of the alginate microspheres and its stability in the physiological environment were investigated. Moreover, the kinetics of the nanoparticles with doxorubicin release from the alginate microspheres covered with chitosan multilayers was characterized, depending on the thickness of the chitosan layer. Further, the cytotoxicity study of the alginate microspheres covered with chitosan multilayer and containing nanoparticles was performed to determine the therapeutic effect of the released nanoparticles with doxorubicin on the HeLa cells during the in vitro cell culture.
    Źródło:
    Chemical and Process Engineering; 2022, 43, 3; 405--417
    0208-6425
    2300-1925
    Pojawia się w:
    Chemical and Process Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Hydrogel antibacterial coating for silicone medical devices
    Autorzy:
    Kopeć, Kamil
    Żuk, Michał
    Ciach, Tomasz
    Powiązania:
    https://bibliotekanauki.pl/articles/2131510.pdf
    Data publikacji:
    2021
    Wydawca:
    Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
    Tematy:
    alginate
    antibacterial coatings
    chitosan
    layer-by-layer
    lysozyme
    medical devices
    Opis:
    Effective antibacterial coatings are in demand in medicine, especially for urological medical devices such as catheters and stents. We propose the production method of an antibacterial hydrogel coating on polydimethylsiloxane (PDMS, silicone), a popular surface for medical materials. The coating process consists of the following steps: PDMS surface activation (introduction of hydroxyl groups), silanisation (introduction of amine groups) and application of chitosan/alginate hydrogel with the addition of lysozyme as an antibacterial agent using the layer-by-layer method. We investigated the effect of polyion concentration on the coating mass, swelling ratio and stability. We analysed the adsorption of Micrococcus luteus, Escherichia coli and Proteus rettgeri on a PDMS surface using confocal laser scanning microscopy. The chitosan/alginate hydrogel coating with immobilised lysozyme protected the PDMS surface against adhesion for all three tested bacterial strains.
    Źródło:
    Progress on Chemistry and Application of Chitin and its Derivatives; 2021, 26; 135-147
    1896-5644
    Pojawia się w:
    Progress on Chemistry and Application of Chitin and its Derivatives
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Expression of the gene encoding blood coagulation factor VIII without domain B in E. coli bacterial expression system
    Autorzy:
    Mazurkiewicz-Pisarek, Anna
    Mazurkiewicz, Alina
    Mikiewicz, Diana
    Baran, Piotr
    Ciach, Tomasz
    Powiązania:
    https://bibliotekanauki.pl/articles/16706242.pdf
    Data publikacji:
    2023
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    factor VIII
    hemophilia type A
    recombinant coagulation factor VIII
    prokaryotic expression system
    E. coli
    recombinant protein production system
    Opis:
    In this article, we have demonstrated the feasibility of generating an active form of recombinant blood coagulation factor VIII using an E. coli bacterial expression system as a potential treatment for hemophilia type A. Factor VIII (FVIII), an essential blood coagulation protein, is a key component of the fluid phase blood coagulation system. So far, all available recombinant FVIII formulations have been produced using eukaryotic expression systems. Mammalian cells can produce catalytically active proteins with all the necessary posttranslational modifications. However, cultivating such cells is time-consuming and highly expensive, and the amount of the obtained product is usually low. In contrast to eukaryotic cells, bacterial culture is inexpensive and allows the acquisition of large quantities of recombinant proteins in a short time. With this study, we aimed to obtain recombinant blood coagulation factor VIII using the E. coli bacterial expression system, a method not previously explored for this purpose. Our research encompasses the synthesis of blood coagulation factor VIII and its expression in a prokaryotic system. To achieve this, we constructed a prokaryotic expression vector containing a synthetic factor VIII gene, which was then used for the transformation of an E. coli bacterial strain. The protein expression was confirmed by mass spectrometry, and we assessed the stability of the gene construct while determining the optimal growth conditions. The production of blood coagulation factor VIII by the E. coli bacterial strain was carried out on a quarter-technical scale. We established the conditions for isolation, denaturation, and renaturation of the protein, and subsequently confirmed the activity of FVIII.
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2023, 104, 3; 247-262
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Biomimetic alginate/perfluorocarbon microcapsules - tthe effect on in vitro metabolic activity and long-term cell culture
    Autorzy:
    Stefanek, Agata
    Kulikowska-Darłak, Aleksandra
    Bogaj, Karolina
    Nowak, Aleksandra
    Dembska, Joanna
    Ciach, Tomasz
    Powiązania:
    https://bibliotekanauki.pl/articles/2173417.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    alginian
    mikrokapsułki
    hodowla komórkowa
    perfluorowęglowodory
    metabolizm
    alginate
    microcapsules
    cell culture
    perfluorocarbons
    metabolism
    Opis:
    Cell encapsulation seems to be a promising tool in tissue engineering. However, it has been shown to have several limitations in terms of long-term cell cultures due to an insufficient oxygen supply. In this study we propose the use of novel microcapsules designed for long-term cell culture consisting of an alginate shell and perfluorocarbon (PFC) core, which works as a synthetic oxygen carrier and reservoir. The influence of PFC presence in the culture as well as the size of structures on cell metabolism was evaluated during 21-day cultures in normoxia and hypoxia. We showed significant improvement in cell metabolism in groups where cells were encapsulated in hydrogel structures with a PFC core. The cells maintained a typical metabolism (oxidative phosphorylation) through all 21 days of the culture, overcoming the oxygen supply shortage even in large structures (diameter ¡ 1 mm). Applying PFC in alginate matrices can improve cell metabolism and adaptation in long-term cell cultures.
    Źródło:
    Chemical and Process Engineering; 2022, 43, 1; 81--95
    0208-6425
    2300-1925
    Pojawia się w:
    Chemical and Process Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-7 z 7

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