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Tytuł:
Challenges in Acanthamoeba keratitis – treatment efficacy of a sight-threatening disease in a severe case without identified risk factors requiring diagnostic verification
Autorzy:
Kuligowska, A.
Baltaza, W.
Chomicz, L.
Machalińska, A.
Powiązania:
https://bibliotekanauki.pl/articles/28761943.pdf
Data publikacji:
2021
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2021, 28, 4; 719-723
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The first investigation on differences in the effectiveness of mycophenolate mofetil and azathioprine antimetabolites determined in Polish patients treated for non-infectious uveitis
Autorzy:
Waszczyk-Łączak, M.
Łazicka-Gałecka, M.
Chomicz, L.
Mucha, K.
Pączek, L.
Szaflik, J.P.
Powiązania:
https://bibliotekanauki.pl/articles/28762883.pdf
Data publikacji:
2020
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2020, 27, 4; 644-649
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains
Autorzy:
Padzik, M.
Hendiger, E.B.
Żochowska, A.
Szczepaniak, J.
Baltaza, W.
Pietruczuk-Padzik, A.
Olędzka, G.
Chomicz, L.
Powiązania:
https://bibliotekanauki.pl/articles/2082951.pdf
Data publikacji:
2019
Wydawca:
Instytut Medycyny Wsi
Tematy:
Acanthamoeba keratitis
contact lens solutions
silver/gold nanoparticles
Acanthamoeba trophozoites
anti-amoebic in
vitro effect
Opis:
Introduction. Various Acanthamoeba species are free-living organisms widely distributed in the human environment. Amphizoic amoebae as facultative parasites may cause vision-threatening eye disease – Acanthamoeba keratitis, mostly among contact lens wearers. As the number of cases is increasing, and applied therapy often unsuccessful, proper hygienic measures and effective contact lenses disinfection are crucial for the prevention of this disease. Available contact lens solutions are not fully effective against amphizoic amoebae; there is a need to enhance their disinfecting activity to prevent amoebic infections. The use of developing nanotechnology methods already applied with success in the prevention, diagnostic and therapy of other infectious diseases might be helpful regarding amoebic keratitis. This study assesses the in vitro effect of selected contact lens solutions conjugated with nanoparticles against Acanthamoeba trophozoites. Materials and method. Three selected contact lens solutions conjugated with silver and gold nanoparticles in concentration of 0.25–2.5 ppm were used in vitro against the axenically cultured ATCC 30010 type Acanthamoeba castellanii strain. The anti-amoebic efficacy was examined based on the oxido-reduction of AlamarBlue. The cytotoxicity tests based on the measurement of lactate dehydrogenase (LDH) activity were performed using a fibroblast HS-5 cell line. Results. Enhancement of the anti-amoebic activity of contact lens solutions conjugated with selected nanoparticles expressed in the dose dependent amoebic growth inhibition with a low cytotoxicity profile was observed. Conclusions. Results of the study showed that conjugation of selected contact lens solutions with silver nanoparticles might be a promising approach to prevent Acanthamoeba keratitis among contact lens users.
Źródło:
Annals of Agricultural and Environmental Medicine; 2019, 26, 1; 198-202
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of in vitro effects of selected physical and chemical agents on detected in Poland Acanthamoeba strains - factors of increasing threats for public health
Autorzy:
Baltaza, W.
Padzik, M.
Szaflik, J.P.
Perkowski, K.
Dybicz, M.
Chomicz, L.
Powiązania:
https://bibliotekanauki.pl/articles/6390.pdf
Data publikacji:
2019
Wydawca:
Polskie Towarzystwo Parazytologiczne
Źródło:
Annals of Parasitology; 2019, 65, 1
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Examination of oral biofilm microbiota in patients using fixed orthodontic appliances in order to prevent risk factors for health complications
Autorzy:
Perkowski, K.
Balraza, W.
Conn, D.B.
Marczyńska-Stolarek, M.
Chomicz, L.
Powiązania:
https://bibliotekanauki.pl/articles/2083064.pdf
Data publikacji:
2019
Wydawca:
Instytut Medycyny Wsi
Tematy:
orthodontical appliances
infectious oral microbiota
risk of health complications
prevention
Opis:
Introduction and objective. In recent decades the use of orthodontic appliances in Poland has increased; however, data on their influence on changes of components of the microbiome connected with oral biofilm are scarce. The objective of this study was to evaluate oral microbiota in terms of their role as risk factors for health complications. Materials and method. The study included 100 patients treated with removable or fixed appliances. Oral hygiene and gingival health were determined, and periodontal swabs taken from each patient for parasitological, bacteriological and mycological microscopic and in vitro examinations. Results. Oral protists and various pathogenic and opportunistic bacterial and fungal strains were identified in the superficial layer of biofilm. A higher prevalence of bacteria, Enterococcus faecalis, E. faecium, Staphylococcus aureus and Escherichia coli, and various strains of yeast-like fungi from the Candida albicans group, occurred in patients treated with the fixed appliance than in those using a removable appliance or not treated orthodontically. In some periodontal samples from patients treated with fixed appliances, cysts of the Acanthamoeba spp. were found. Conclusions. The use of orthodontic appliances alters the status of the oral cavity; it has impact on the colonization of oral biofilm by opportunistic/pathogenic strains, and increases the risk of their dissemination to various human tissues and organs. Pretreatment examination of oral microbiome, its monitoring particularly during treatment with fixed appliances, and preventive elimination of the potentially pathogenic strains to avoid health complications, are highly recommended, especially in patients with impaired immunity.
Źródło:
Annals of Agricultural and Environmental Medicine; 2019, 26, 2; 231-235
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparative examination on selected amphizoic amoebae in terms of their in vitro temperature tolerance - a possible indirect marker of potential pathogenicity of Acanthamoeba strains
Autorzy:
Baltaza, W.
Padzik, M.
Szaflik, J.P.
Dybicz, M.
Grochowska, A.
Kuryłowicz, A.
Chomicz, L.
Powiązania:
https://bibliotekanauki.pl/articles/5852.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Parazytologiczne
Źródło:
Annals of Parasitology; 2018, 64, 4
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparison of chlorhexidine disinfectant in vitro effect on environmental and ocular Acanthamoeba strains, the amoebic agents of human keratitis - an emerging sight-threatening corneal disease in Poland
Autorzy:
Padzik, M.
Baltaza, W.
Szaflik, J.P.
Hendiger, E.
Dybicz, M.
Chomicz, L.
Powiązania:
https://bibliotekanauki.pl/articles/6211.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Parazytologiczne
Źródło:
Annals of Parasitology; 2018, 64, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of povidone iodine, chlorhexidine digluconate and toyocamycin on amphizoic amoebic strains, infectious agents of Acanthamoeba keratitis – a growing threat to human health worldwide
Autorzy:
Padzik, M.
Baltaza, W.
Conn, D.B.
Szaflik, J.P.
Chomicz, L.
Powiązania:
https://bibliotekanauki.pl/articles/2081938.pdf
Data publikacji:
2018
Wydawca:
Instytut Medycyny Wsi
Tematy:
in vitro
Acanthamoeba Keratitis
A. polyphaga
T4 genotype
A. castellanii Neff
povidone iodine
chlorhexidine digluconate
toyocamycin effects
Opis:
Introduction. Free-living amoebae, ubiquitous in outer environments, in predisposing circumstances may exist as parasites, infectious agents of Acanthamoeba keratitis. In recent decades, the vision-threatening corneal infection is a growing human health threat worldwide, including Poland. The applied therapy is often ineffective due to diagnostic mistakes, various pathogenicity of Acanthamoeba strains and high resistance of cysts to drugs; many agents with possible anti-amoebic activity are still being tested. In the presented study, selected chemicals are investigated in terms of their in vitro effect on corneal and environmental Acanthamoeba strains. Materials and method. Samples of a corneal isolate from a patient with severe Acanthamoeba keratitis,of assessed on the basis of genotype associations of 18S rRNA and the type strain, Acanthamoeba castellanii Neff cultivated in bacteria-free condition, were exposed to povidone iodine, chlorhexidine digluconate or toyocamycin. In vitro population dynamics of the strains were monitored and compared to those of control cultures. Results. All chemicals showed anti-amoebic effects with different degrees of effectiveness. Significant differences were observed in the in vitro population dynamics, and the morpho-physiological status of A. castellanii Neff T4 and corneal strains determined as A. polyphaga T4 genotype, exposed to povidone iodine or toyocamycin, in comparison with chlorhexidine taken as reference. Conclusions. Time-dependent amoebstatic in vitro effects were demonstrated for all agents, in particular, the results of assays with povidone iodine are promising. No significant stimulation of encystation appeared; however, as cysticidal efficacy of chemicals is expected, complementary research is needed on different Acanthamoeba strains with modified agent concentrations and method application.
Źródło:
Annals of Agricultural and Environmental Medicine; 2018, 25, 4; 725-731
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular determination of suspected alveolar echinococcosis requiring surgical treatment in human cases from Poland
Autorzy:
Dybicz, M.
Borkowski, P.K.
Padzik, M.
Baltaza, W.
Chomicz, L.
Powiązania:
https://bibliotekanauki.pl/articles/5686.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Parazytologiczne
Źródło:
Annals of Parasitology; 2018, 64, 4
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular identification of Trichomonas tenax in the oral environment of domesticated animals in Poland – potential effects of host diversity for human health
Autorzy:
Dybicz, M.
Perkowski, K.
Baltaza, W.
Padzik, M.
Sędzikowska, A.
Chomicz, L.
Powiązania:
https://bibliotekanauki.pl/articles/2081757.pdf
Data publikacji:
2018
Wydawca:
Instytut Medycyny Wsi
Tematy:
PCR
specificity
sequencing
Trichomonas tenax
oral trichomonosis
epidemiology
Polska
domesticated animals
humans
Opis:
Introduction. The protozoan Trichomonas tenax is considered to be a human specific flagellate of the oral cavity, found in humans with poor oral hygiene and advanced periodontal disease. Morphological variability and great similarity between species occurring in humans and animals, complicate the specific identification of trichomonads, using microscopic examination and other standard parasitological techniques. Objective. The aim of the study was to search for and identify T. tenax in domesticated animals using molecular methods. The obtained data were assessed in terms of potential effects of a spread of the species deriving from the animals in the human environment. Materials and method. 301 animals: 142 dogs, 57 cats and 102 horses, were examined in terms of their mouth status and occurrence of trichomonads. ITS1–5.8S rRNA-ITS2 region was amplified and sequenced. Results. Finally, 7 dogs, 3 cats and 1 horse were diagnosed positive for T. tenax by PCR. In the oral cavity of 9 /11 animals, gingivitis and dental plaque accumulation were diagnosed. 9 /11 sequences of trichomonad isolates showed 100% identity with T. tenax sequence derived from the GenBank. The sequences of 2 isolates differed by substitutions. Conclusions. It was proved that T. tenax, considered so far as a human specific parasite, can also inhabit the oral cavity of dog, cat and horse. To summarize, T. tenax was detected in the mouths of different domesticated animals, indicating that in Poland it can colonize a wider range of hosts than previously known. The owners of 3 dogs showed oral tissue inflammation of different intensity and were also positive for T. tenax; therefore, oral trichomonosis spread from humans to domestic animals and conversely should be taken into consideration.
Źródło:
Annals of Agricultural and Environmental Medicine; 2018, 25, 3; 464-468
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Studies on prevalence of infection with Trichomonas tenax identified by molecular techniques - in respect to oral health of patients with various systemic disease requiring immunosuppressive therapy
Autorzy:
Dybicz, M.
Perkowski, K.
Sedzikowska, A.
Baltaza, W.
Chomicz, L.
Powiązania:
https://bibliotekanauki.pl/articles/6194.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Parazytologiczne
Źródło:
Annals of Parasitology; 2018, 64, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Assessment of in vitro dynamics of pathogenic Acanthamoeba strains originating from contact lens wearers with infectious keratitis
Autorzy:
Padzik, M.
Starosciak, B.
Szaflik, J.P.
Pietruczuk- Padzik, A.
Siczek, P.
Chomicz, L.
Powiązania:
https://bibliotekanauki.pl/articles/5537.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Opis:
Recently, incidents of Acanthamoeba keratitis, the vision-threatening eye disease, are reported with increasing frequency worldwide, particularly in contact lens wearers. In our study, the retrospective assessment of in vitro dynamics of subsequent pathogenic Acanthamoeba isolates cultured at 24°C, detected in Polish contact lens wearers with keratitis is presented and results compared with those of environmental A. castellanii Neff strain. There were delayed the proper diagnosis that influenced prolonged and severe course of this eye disease and treatment difficulties. The corneal material was examined directly to visualize developmental amoeba stages for diagnose verification, microbiologically tested for the specific identification of bacteriae and fungi, and in vitro grown in culture medium in temperature 24°C. Among twenty-six keratitis incidents analyzed, eleven were cases of Acanthamoeba keratitis; in the six of them, Acanthamoeba strains and concomitant bacterial and/or fungal infectious agents were detected. In vitro assays showed variability in population density of several clinical strains in the exponential growth phase expressed in various range of overall amoeba number and different proportion between trophozoites and cysts. The clear influence of temperature on the in vitro cultivation of the amoebae was observed: statistically significant lower population dynamics was revealed by most of pathogenic clinical isolates in comparison with those showed by environmental strain. The in vitro monitoring of dynamics of Acanthamoeba strains isolated from infected eyes may be helpful for diagnostics verification, especially in mixed infectious keratitis.
Źródło:
Annals of Parasitology; 2016, 62, 4
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparative assessment of selected intraoral microorganisms – potential factors for peri-surgical management complications
Autorzy:
Zawadzki, P.J
Starosciak, B.
Perkowski, K.
Baltaza, W.
Padzik, M.
Pionkowski, K.
Chomicz, L.
Powiązania:
https://bibliotekanauki.pl/articles/6223.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Opis:
In this research, a comparative analysis of results of investigations involving different human populations, in terms of a relation between the oral cavity health and the species composition of mouth microbiota is reported. The purpose of this analysis was to identify and assess microorganisms that could cause health complications in patients with neoplasm requiring dental problem-related surgical management. The patients with the oral cancer surgically treated and those without neoplasm were assessed for their oral health: status of teeth, gingiva, periodontium, and occurrence of inflammatory processes. From each patient, microorganisms isolated of periodontium, dental plaque, and dental pocket swabs were identified in wet and stained microscopic preparations; standard microbiology in vitro techniques were also applied to determine the fungal and bacterial strains. The comparative analysis of results of direct microscopic examinations and in vitro cultures assessment indicated significant differences in prevalences of fungi, parasitic oral protozoans and bacteriae in particular patient’s groups. Yeast-like fungi belonging to Candida genus, mostly of C. albicans group, were identified in 93.75% patients with the oral cancer, while in 25% of individuals assessed without neoplasm. E. gingivalis amoebae were only found in 12.5% patients with the serious disease; no trichomonads were detected in all patients analyzed. Among bacteria species, potentially pathogenic Enterobacteriaceae were found in the patients with oral cancer. The pronounced shift in the microbiota species composition in the patients who needed prolonged treatment due to oral cavity cancer, compared to other generally healthy persons has been showed in this analysis.
Źródło:
Annals of Parasitology; 2016, 62, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of selected oral cavity microbiota – risk factors of management complications in patients with masticatory system disorders
Autorzy:
Zawadzki, P.J.
Perkowski, K.
Starosciak, B.
Dybicz, M.
Baltaza, W.
Pionkowski, K.
Chomicz, L.
Powiązania:
https://bibliotekanauki.pl/articles/6035.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Opis:
The retrospective analysis of data on oral cavity clinical status in relation to microbiota species composition is presented. The research regards patients of different age, with and without congenital malformation, pretreatment assessed for occurrence of pathological changes in the masticatory system. Samples of the swabs collected from each patient (from dental plaque, periodontium and dental pockets) were used for identification of oral protozoans in wet slides and stained preparations; additionally, transmission electron microscope examination was performed. The material was used for in vitro cultures to identify bacteria strains. Clinically, intensity of tissue deteriorations was higher in patients with a congenital disease. Alive Trichomonas tenax and Entamoeba gingivalis, species with confirmed pathogenic impact on oral cavity and neighboring structures, were detected with higher prevalence in older patients. Enterococci, Staphylococcus aureus, various Enterobacteriaceae were more frequently detected in patients with somatic and mental retardations; in mouths of those patients, Klebsiella pneumonia and Pseudomonas aeruginosa opportunistic strains occurred. Masticatory system abnormalities favor colonization of oral cavity by exogenous species and dissemination of infections, especially dangerous for patients with congenital diseases. Oral microbiota assessment and preventive measures may be helpful to avoid subsequent peri-surgery complications.
Źródło:
Annals of Parasitology; 2016, 62, 1
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular characterisation of Echinococcus granulosus sensu stricto cases in Polish patients
Autorzy:
Dybicz, M.
Borkowski, P.K.
Dabrowska, J.
Chomicz, L.
Powiązania:
https://bibliotekanauki.pl/articles/6595.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
molecular characteristics
Echinococcus granulosus
human disease
cystic echinococcosis
patient
liver
hip muscle
surgery
Polska
Źródło:
Annals of Parasitology; 2016, 62, Suppl.
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 29

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