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    Wyświetlanie 1-9 z 9
    Tytuł:
    Sp100 interacts with phage ΦC31 integrase to inhibit its recombination activity
    Autorzy:
    Lin, Yun
    Li, Zhi-Hui
    Wang, Jing-Jing
    Xu, Gua-Lan
    Shen, Qi
    Tian, Lin
    Xue, Jin-Lun
    Chen, Jin-Zhong
    Powiązania:
    https://bibliotekanauki.pl/articles/1039951.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Sp100
    ΦC31 integrase
    recombination
    Opis:
    Phage ΦC31 integrase is a potential vector for the insertion of therapeutic genes into specific sites in the human genome. To understand the mechanism involved in ΦC31 integrase-mediated recombination, it is important to understand the interaction between the integrase and cellular proteins. Using a yeast two-hybrid system with pLexA-ΦC31 integrase as bait, we screened a pB42AD human fetal brain cDNA library for potential interacting cellular proteins. From the 106 independent clones that were screened, 11 potential interacting clones were isolated, of which one encoded C-terminal fragment of Sp100. The interaction between Sp100 and ΦC31 integrase was further confirmed by yeast mating and co-immunoprecipitation assays. The hybridization between a ΦC31 integrase peptide array and an HEK293 cell extract revealed that residues 81RILN84 in the N-terminus of ΦC31 integrase are responsible for the interaction with Sp100. Knocking down endogenous Sp100 with Sp100-specific siRNA increased ΦC31 integrase-mediated recombination but did not impact reporter gene expression. Therefore, endogenous Sp100 may interact with ΦC31 integrase and inhibit the efficiency of ΦC31 integrase-mediated recombination.
    Źródło:
    Acta Biochimica Polonica; 2011, 58, 1; 67-73
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Characterization of recombinant expression of Bombyx mori bidensovirus ns1 using a modified vector
    Autorzy:
    Li, Guohui
    Li, Mangmang
    Wang, Peng
    Hu, Zhaoyang
    Yao, Qin
    Tang, Qi
    Chen, Keping
    Powiązania:
    https://bibliotekanauki.pl/articles/1039216.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    BmBDV
    NS1
    baculovirus expression vector
    Sf9
    egfp
    Opis:
    ns1 gene of Bombyx mori bidensovirus (BmBDV) consisted of 951 nucleotides encoding a deduced 316-amino aicd protein. In this study, the gene was cloned and fused in frame with a N-terminal 6×His tag under control of the polyhedrin promoter, which was transposed into the mini-attTn7 locus of a modified baculovirus vector. Transfection of Sf-9 cells with the resulting recombinant DNA was performed to prepare recombinant virus and the resultant supernatant of transfection with fluorescent signal was harvested. Western blot analysis revealed that NS1 protein was successfully expressed in Sf9 cells infected with the recombinant virus and was confirmed by LC-MS/MS analysis. Moreover, the expressed NS1 is a phosphorylated protein and the phosphorylation site is Thr-184. These results showed that the activity of BmBDV NS1 may be regulated by phosphorylation.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 4; 787-794
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Elastic anisotropic and thermodynamic properties of I-4m2-BCN
    Autorzy:
    Xing, Mengjiang
    Li, Binhua
    Yu, Zhengttao
    Chen, Qi
    Powiązania:
    https://bibliotekanauki.pl/articles/1065017.pdf
    Data publikacji:
    2016-06
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    62.20.de
    63.20.dk
    65.40.-b
    71.15.Mb
    Opis:
    Based on the density functional theory and the quasi-harmonic Debye model, the structural and thermodynamic properties of I-4m2-BCN have been studied in this paper. Some structural parameters are presented in this work. All of these results are in excellent agreement with the other available results. The anisotropy of elastic properties are also studied systematically in this paper. Finally, the thermodynamic properties of I-4m2-BCN are also researched through the quasi-harmonic Debye model. The relations among the thermal expansion α, the Debye temperature Θ_{D}, the heat capacity C_V and C_P, the Grüneisen parameter γ , entropy S, and the Gibbs free energy G with pressure P and temperature T are studied systematically.
    Źródło:
    Acta Physica Polonica A; 2016, 129, 6; 1124-1130
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Elastic Anisotropic and Thermodynamic Properties of Two BC₇ Phases
    Autorzy:
    Xing, Mengjiang
    Li, Binhua
    Yu, Zhengtao
    Chen, Qi
    Powiązania:
    https://bibliotekanauki.pl/articles/1032568.pdf
    Data publikacji:
    2017-10
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    ab initio
    elastic properties
    anisotropic properties
    thermodynamic properties
    Opis:
    The structural, elastic, anisotropic, and thermodynamic properties of P3m1-BC₇ and Pmm2-BC₇ have been studied in this paper utilizing first-principles calculations. In comparison with the elastic properties of Pmm2-BC₇, P3m1-BC₇ exhibits slightly higher values in bulk modulus and B/G, with similar values in shear modulus, the Young modulus, and the Poisson ratio. The calculated Pugh modulus ratio (B/G) and the Poisson ratio demonstrates P3m1-BC₇ from brittle to ductile at 93.60 and 93.73 GPa, respectively. Calculations of shear anisotropic factor, universal elastic anisotropy index, shear modulus, the Young modulus, and the Poisson ratio for BC₇ then demonstrate that Pmm2-BC₇ exhibits a larger elastic anisotropy than P3m1-BC₇. Quasi-harmonic Debye model is finally applied to investigate the Debye temperature, the coefficient of thermal expansion, heat capacity and Grüneisen parameter of Pmm2-BC₇ and P3m1-BC₇.
    Źródło:
    Acta Physica Polonica A; 2017, 132, 4; 1340-1346
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Lipopolysaccharide accelerates fine particulate matter-induced cell apoptosis in human lung bronchial epithelial cells
    Autorzy:
    Ru, Qin
    Xiong, Qi
    Chen, Lin
    Tian, Xiang
    Yue, Kai
    Ma, Baomiao
    Liu, Lu
    Wu, Rihui
    Xu, Congyue
    Pi, Mingshan
    Li, Chaoying
    Powiązania:
    https://bibliotekanauki.pl/articles/2161782.pdf
    Data publikacji:
    2017-11-03
    Wydawca:
    Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
    Tematy:
    Apoptosis
    cytotoxicity
    inflammation
    fine particulate matter
    free radicals
    bronchial epithelial cells
    Opis:
    Objectives The aim of the study has been to investigate the effect of the Standard Reference Material of fine particulate matter (SRM 2786) on cytotoxicity and apoptosis in human lung bronchial epithelial cells (16HBE cells). Whether the lipopolysaccharide (LPS)-induced inflammation could further accelerate cell apoptosis induced by SRM 2786 stimulation has also been determined. Material and Methods 16HBE cells were exposed to various doses of SRM 2786 with or without LPS. The following parameters: cytotoxicity, apoptotic rate, Bax/Bcl-2 expression, nitric oxide (NO) production, and reactive oxygen species (ROS) generation were measured. Results The results have shown that SRM 2786 induces cell damage and apoptosis of 16HBE cells as demonstrated by significant decrease in expression of Bcl-2 and increase in expression of Bax. When compared with the control cells, the apoptotic rate of cells treated by 500 μg/ml of SRM 2786 increased from 2.43±0.21% to 43.96±2.95% (p < 0.01). Further, there was an elevated production of NO and ROS post SRM 2786 treatment. The level of NO in cells treated with 500 μg/ml of SRM 2786 was 18.33±1.02 μmol/l whereas that of control cells was 1.58±0.31 μmol/l (p < 0.01). When compared with the control group, the level of intracellular ROS increased by 24% after treatment with 500 μg/ml of SRM 2786 (p < 0.05). In addition, LPS pre-treatment may accelerate cell apoptosis by increasing generation of NO and ROS followed by SRM 2786 stimulation. When compared to cells treated with 125 μg/ml of SRM 2786 alone, the levels of NO and ROS in cells pretreated with LPS increased by 28% and 11.6%, respectively (p < 0.05), and the apoptotic rate increased from 34.62±4.44% to 54.11±3.34% (p < 0.01). Conclusions These findings have suggested that in vitro exposure to SRM 2786 could induce 16HBE cells apoptosis probably by means of the mechanism involving the generation of free radicals, while the degree of apoptosis would be further aggravated under inflammation condition. Int J Occup Med Environ Health 2018;31(2):173–183
    Źródło:
    International Journal of Occupational Medicine and Environmental Health; 2018, 31, 2; 173-183
    1232-1087
    1896-494X
    Pojawia się w:
    International Journal of Occupational Medicine and Environmental Health
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Adaptive filtering-based current reconstruction in non-contact magnetic sensor array measurement system
    Autorzy:
    Chen, Yafeng
    Huang, Qi
    Powiązania:
    https://bibliotekanauki.pl/articles/221214.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    current measurement
    adaptive filtering
    sensor array
    gauss white noise
    Opis:
    The non-contact current measurement method with magnetic sensors has become a subject of research. Unfortunately, magnetic sensors fail to distinguish the interested magnetic field from nearby interference and suffer from gauss white noise due to the intrinsic noise of the sensor and external disturbance. In this paper, a novel adaptive filtering-based current reconstruction method with a magnetic sensor array is proposed. Interference-rejection methods based on two classic algorithms, the least-mean-square (LMS) and recursive-least-square (RLS) algorithms, are compared when used in the parallel structure and regular triangle structure of three-phase system. Consequently, the measurement range of RLS-based algorithm is wider than that of LMS-based algorithm. The results of carried out simulations and experiments show that RLS-based algorithms can measure currents with an error of around 1%. Additionally, the RLS-based algorithm can filter the gauss white noise whose magnitude is within 10% of the linear magnetic field range of the sensor.
    Źródło:
    Metrology and Measurement Systems; 2019, 26, 4; 697-711
    0860-8229
    Pojawia się w:
    Metrology and Measurement Systems
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The CFD method-based research on damaged ship’s flooding process in time-domain
    Autorzy:
    Hu, Li-Fen
    Qi, Huibo
    Li, Yuemeng
    Li, Wubin
    Chen, Shude
    Powiązania:
    https://bibliotekanauki.pl/articles/259812.pdf
    Data publikacji:
    2019
    Wydawca:
    Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
    Tematy:
    flooding process
    Bernoulli equation
    time domain calculation
    damaged ship
    Opis:
    The flooding process is one of the main concerns of damaged ship stability. This paper combines the volume of fluid (VOF) method incorporated in the Navier-Stokes (NS) solver with dynamic mesh techniques to simulate the flooding of a damaged ship. The VOF method is used to capture the fluid interface, while the dynamic mesh techniques are applied to update the mesh as a result of transient ship motions. The time-domain flooding processes of a damaged barge and a rectangular cabin model are carried out based on the abovementioned method, and the computational results appear compatible with the experimental data. During the flooding process, the motion of the flooding flow at different stages is observed and compared with that observed in real conditions. The time-domain research of the flooding process is the starting point for subsequent establishment of damaged ship’s roll movement and capsizing the mechanism of dead ship condition in wave.
    Źródło:
    Polish Maritime Research; 2019, 1; 72-81
    1233-2585
    Pojawia się w:
    Polish Maritime Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Optimization of Al-Cu Cast Alloy Composition for Hydraulic Valves
    Autorzy:
    Li, Rong
    Chen, Lunjun
    Zeng, Qi
    Su, Ming
    Xie, Zhiping
    Powiązania:
    https://bibliotekanauki.pl/articles/381745.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Al-Cu alloy
    optimization
    strengthening mechanisms
    hydraulic multi-way valve
    stop Al-Cu
    optymalizacja
    mechanizm wzmacniający
    zawór hydrauliczny
    Opis:
    In order to identify the influence of different Mn, Cd, V and Zr content on the properties of Al-Cu casting alloys in hydraulic valves, orthogonal test methods were used to prepare alloy test bars with different elements and contents. Tensile tests were performed on the test bars so obtained. The microstructure of alloys with different compositions is studied. The results show that adding approximately 0.4% of Mn can not only form a strengthening phase but also reduce the excessive segregation of the matrix along the grain boundary. A Cd content of 0.2% can promote the formation of micro Cd spheres in the softer aluminum matrix. Hard spots increase the wear resistance of the material; however, an excess of Cd will cause element segregation and deteriorate the mechanical properties of the valve body. Zr and V refine the grains in the alloy; however, an excess of these elements will lead to a large area of segregation. If proper heat treatment is lacking, the mechanical properties of the valve body deteriorate.
    Źródło:
    Archives of Foundry Engineering; 2020, 20, 1; 84-94
    1897-3310
    2299-2944
    Pojawia się w:
    Archives of Foundry Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Numerical study on the dynamic response of a concrete filled steel tubular long column under axial impact by a rigid-body
    Autorzy:
    Qi, Han
    Chen, Shuohui
    Zou, Jianlei
    Zhang, Huidi
    Liu, Mengsha
    Ju, Jinsan
    Sang, Xiuxing
    Powiązania:
    https://bibliotekanauki.pl/articles/2055059.pdf
    Data publikacji:
    2021
    Wydawca:
    Polskie Towarzystwo Mechaniki Teoretycznej i Stosowanej
    Tematy:
    concrete filled steel tubular long column
    rigid body
    impact load
    dynamic response
    Opis:
    In this study, refined finite element models of concrete filled steel tubular long columns with length diameter ratio (L/D) of 50 were established, and their dynamic responses under a rigid-body impact were explored. It is found that the form of impact load is only related to and can be predicted from the impact velocity of the rigid-body. The change of axial stress and strain with time is synchronous with that of the impact load. Furthermore, the variation characteristics of axial stress and strain with time are mainly related to the initial velocity of the rigid-body.
    Źródło:
    Journal of Theoretical and Applied Mechanics; 2021, 59, 4; 551--563
    1429-2955
    Pojawia się w:
    Journal of Theoretical and Applied Mechanics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-9 z 9

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