- Tytuł:
- Genetic variability of potato spindle tuber viroid RNA replicon.
- Autorzy:
-
Góra-Sochacka, Anna
Candresse, Thierry
Zagórski, Włodzimierz - Powiązania:
- https://bibliotekanauki.pl/articles/1044141.pdf
- Data publikacji:
- 2001
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
genetic stability
sequence diversity
infectious clones
pathogenicity
potato spindle tuber viroid - Opis:
- The genetic continuity of the potato spindle tuber viroid (PSTVd) genome was analysed after infection of tomato plants with cloned cDNAs of parental strains. During the six weeks of the experiment, several new sequence variants appeared. The sequence variants detected in the progeny population induced sequence-specific disease symptoms. The PSTVd genome therefore follows the pattern expected for typical pseudo-strains propagating in plants as a population of similar sequences. Assessing further the replicon continuity, a PSTVd cDNA mutant with a deletion in the central conserved region was constructed and proven to be non-infectious. Surprisingly, in a sub-population of potato transformants expressing the same deleted PSTVd RNA an infectious viroid was detected. This suggests specific transcript conversion followed by recovery of the full-length pathogen genome.
- Źródło:
-
Acta Biochimica Polonica; 2001, 48, 2; 467-476
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł