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Wyświetlanie 1-15 z 15
Tytuł:
The assessment of health (risk) behaviour of academic youth
Autorzy:
Sygit, Katarzyna
Owoc, Alfred
Bojar, Iwona
Powiązania:
https://bibliotekanauki.pl/articles/552402.pdf
Data publikacji:
2011
Wydawca:
Stowarzyszenie Przyjaciół Medycyny Rodzinnej i Lekarzy Rodzinnych
Tematy:
health behaviours
diet
physical activity
stimulants
Źródło:
Family Medicine & Primary Care Review; 2011, 1; 42-50
1734-3402
Pojawia się w:
Family Medicine & Primary Care Review
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Selected conditions of health behaviour of young people from the rural environment
Autorzy:
Sygit, Katarzyna
Owoc, Alfred
Bojar, Iwona
Powiązania:
https://bibliotekanauki.pl/articles/552425.pdf
Data publikacji:
2011
Wydawca:
Stowarzyszenie Przyjaciół Medycyny Rodzinnej i Lekarzy Rodzinnych
Tematy:
young people
rural areas
diet
stimulants
Źródło:
Family Medicine & Primary Care Review; 2011, 1; 51-59
1734-3402
Pojawia się w:
Family Medicine & Primary Care Review
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Aktywność zawodowa i rodzinna kobiet w wieku produkcyjnym niemobilnym
Autorzy:
Raczkiewicz, Dorota
Piasecki, Jakub
Śledziewska, Katarzyna
Bojar, Iwona
Powiązania:
https://bibliotekanauki.pl/articles/610021.pdf
Data publikacji:
2016
Wydawca:
Uniwersytet Marii Curie-Skłodowskiej. Wydawnictwo Uniwersytetu Marii Curie-Skłodowskiej
Tematy:
professional activity
family activity
non-mobility working age
aktywność zawodowa
aktywność rodzinna
wiek produkcyjny niemobilny
Opis:
The aim of this article is to carry out analyses of professional and family activities of women, based on the results of the study “Psychophysical health of women during and after menopause in terms of preserving their ability to work”. This study was conducted in the years 2014–2016 on a sample of 300 women, in the Institute of Rural Health in Lublin on behalf of the Central Institute for Labour Protection in Warsaw. Menopausal and postmenopausal period corresponds to non-mobility working age in economics – that is 45–60 years old. Professional and family activities are the most important in a woman’s life, but they are competing against each other. Professional and family activities of the surveyed women can be assessed positively.
Celem artykułu jest przeprowadzenie analizy aktywności zawodowej i rodzinnej kobiet na podstawie wyników badania pt. „Zdrowie psychofizyczne kobiet w okresie około- i pomenopauzalnym w aspekcie zachowania ich zdolności do pracy”. Badanie to zostało przeprowadzone w latach 2014–2016 na próbie 300 kobiet w Instytucie Medycyny Wsi w Lublinie na zlecenie Centralnego Instytutu Ochrony Pracy w Warszawie. Okres około- i pomenopauzalny odpowiada w ekonomii wiekowi produkcyjnemu niemobilnemu, czyli 45–60 lat. Aktywność zawodowa i rodzinna jest najważniejsza w życiu kobiety, jednak są one konkurencyjne wobec siebie. Aktywność zawodową i rodzinną badanych kobiet można ocenić pozytywnie.
Źródło:
Annales Universitatis Mariae Curie-Skłodowska, sectio H – Oeconomia; 2016, 50, 3
0459-9586
Pojawia się w:
Annales Universitatis Mariae Curie-Skłodowska, sectio H – Oeconomia
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Low frequency electromagnetic waves increase human sperm motility – A pilot study revealing the potent effect of 43 kHz radiation
Autorzy:
Wdowiak, Artur
Mazurek, Paweł A.
Wdowiak, Anita
Bojar, Iwona
Powiązania:
https://bibliotekanauki.pl/articles/2162024.pdf
Data publikacji:
2018-12-20
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
electromagnetic radiation
semen motility
sperm hyperactivation
sperm kinetic variables
metal detecting
infertility
Opis:
Objectives Abnormalities in the timing and course of spermatozoa capacitation and hyperactivation underlie common pathologies related to male infertility. Recent data shows that low frequency electromagnetic waves may influence cell membrane potential and permeability. It is therefore possible that low frequency electromagnetic waves could affect the maturation and motility processes of spermatozoa. The 43-kHz wave generator was used for modeling the impact of environmental exposure to low frequency electromagnetic radiation on human sperm. Material and Methods Sperm samples were gathered from 103 fertile, healthy men aged 25–30 years old and performed computer-assisted sperm analysis. After initial examination, each participant’s semen sample was divided into 2 aliquots (control and experimental) and placed in separate automated incubators. The samples constituting the experimental group were placed into the exposure system that emitted 43-kHz electromagnetic waves. Sperm motility was assessed at 3 h, 12 h and 24 h. Results Exposure to a 43-kHz radio frequency increased the percentage of sperm in progressive motility by up to 5.8% and the velocity of said sperm by up to 2 μm/s. Moreover, the total number of hyperactivated spermatozoa was significantly increased in the semen exposed to the electromagnetic signal. Conclusions In vivo environmental exposure to 43-kHz waves may promote the development of infertility related to premature capacitation outside of the vaginal tract. Exposing semen to this particular frequency may also boost the capacitation and hyperactivation of spermatozoa in vitro, prior to conducting assisted reproductive therapies.Int J Occup Med Environ Health 2018;31(6):723–739
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2018, 31, 6; 723-739
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Quality of life and satisfaction with life of women who are childless by choice
Autorzy:
Bień, Agnieszka
Iwanowicz-Palus, Grażyna
Lecyk, Urszula
Bojar, Iwona
Powiązania:
https://bibliotekanauki.pl/articles/986647.pdf
Data publikacji:
2017
Wydawca:
Instytut Medycyny Wsi
Tematy:
quality of life
satisfaction
voluntary childlessness
women
Opis:
Objective. The study attempts to identify and analyze factors affecting the quality of life and satisfaction with life of women who are childless by choice. Materials and method. The research instruments used were: the WHOQOL-Bref questionnaire and the Satisfaction with Life Scale (SWLS), and author’s own questionnaire. Results. A better overall quality of life, perception of own health and quality of life in all the specific domains analyzed was found among childless women living in cities, college/university-educated, and with a good or very good financial standing. Conclusions. A positive correlation was found between satisfaction with life and overall quality of life, general perceived health, and all quality of life domains. Higher satisfaction with life scores in childless women are correlated with a higher quality of life scores and better perceived health.
Źródło:
Annals of Agricultural and Environmental Medicine; 2017, 24, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Pain involving the motor system and serum vitamin D concentration in postmenopausal women working in agriculture
Autorzy:
Raczkiewicz, Dorota
Owoc, Alfred
Sarecka-Hujar, Beata
Bojar, Iwona
Powiązania:
https://bibliotekanauki.pl/articles/989974.pdf
Data publikacji:
2017
Wydawca:
Instytut Medycyny Wsi
Tematy:
menopause
vitamin d
pain
motor system
Opis:
Introduction and objective. Since the role of vitamin D is essential in numerous biological processes its deficiency was suggested to be a risk factor for e.g. osteoporosis, musculoskeletal pain and spine pain. The purpose of the study was to analyse whether serum vitamin D concentration is related to pain involving the motor system in Polish postmenopausal women working in agriculture. Material and methods. The study group consisted of 1,751 post-menopausal women, aged 45–65, at least 12 months from the last menstrual period, living in rural areas and working in agriculture. The research method was self-assessment of pain involving the motor system using VAS, laboratory test of serum vitamin D concentration and a medical interview. Statistical methods included generalized linear models, analysis of variance, t test for two means in two independents, χ2 test of stochastic independence. Results. Postmenopausal women working in agriculture and suffering from pain in at least one part of the motor system were younger and lower educated, they also had higher abdominal obesity and lower serum vitamin D, compared to those without pain in any part of the motor system. Decreased serum vitamin D concentration in postmenopausal women working in agriculture is important from the aspect of a higher prevalence of pain in the thoracic spine and more severe pain in the neck spine, but not for severity of pain in the lumbar spine; higher occurrence of pain in both hands or wrists; higher prevalence and more severe pain in at least one knee; and no prevalence or severity of pain in the shoulders and elbows. Conclusion. Serum vitamin D concentration is important for the prevalence and severity of pain in the neck and thoracic spine, knees and hands or wrists, but not for the lumbar spine, shoulders and elbows.
Źródło:
Annals of Agricultural and Environmental Medicine; 2017, 24, 1
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of electromagnetic waves on human reproduction
Autorzy:
Wdowiak, Artur
Mazurek, Paweł A.
Wdowiak, Anita
Bojar, Iwona
Powiązania:
https://bibliotekanauki.pl/articles/989982.pdf
Data publikacji:
2017
Wydawca:
Instytut Medycyny Wsi
Tematy:
human reproduction
electromagnetic waves
infertility
exposure to electromagnetic fields
Opis:
Electromagnetic radiation (EMR) emitting from the natural environment, as well as from the use of industrial and everyday appliances, constantly influence the human body. The effect of this type of energy on living tissues may exert various effects on their functioning, although the mechanisms conditioning this phenomenon have not been fully explained. It may be expected that the interactions between electromagnetic radiation and the living organism would depend on the amount and parameters of the transmitted energy and type of tissue exposed. Electromagnetic waves exert an influence on human reproduction by affecting the male and female reproductive systems, the developing embryo, and subsequently, the foetus. Knowledge concerning this problem is still being expanded; however, all the conditionings of human reproduction still remain unknown. The study presents the current state of knowledge concerning the problem, based on the latest scientific reports.
Źródło:
Annals of Agricultural and Environmental Medicine; 2017, 24, 1
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Practical measures of cognitive function and promotion of their performance in the context of research
Autorzy:
Gujski, Mariusz
Juńczyk, Tomasz
Pinkas, Jaroslaw
Owoc, Alfred
Bojar, Iwona
Powiązania:
https://bibliotekanauki.pl/articles/989037.pdf
Data publikacji:
2016
Wydawca:
Instytut Medycyny Wsi
Tematy:
cognitive function
cognitive reserve
prevention
training programmes
Opis:
The aging of the population generates a number of very interesting research questions in the fields of medicine, psychology, sociology, demography, and many others. One of the issues subject to both intensive research by scientists and exploration by practitioners is associated with cognitive functions. The article presents current knowledge regarding practical actions in the field of promoting cognitive function using diagnostic programmes and training using modern technologies. An important aspect presented in this study is also related to the welfare of the maintenance or improvement of cognitive function. Information and communication technologies will contribute to the dissemination of computerized cognitive training, also personalized.
Źródło:
Annals of Agricultural and Environmental Medicine; 2016, 23, 3
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Impact of spinal pain on daily living activities in postmenopausal women working in agriculture
Autorzy:
Raczkiewicz, Dorota
Owoc, Alfred
Sarecka-Hujar, Beata
Saran, Tomasz
Bojar, Iwona
Powiązania:
https://bibliotekanauki.pl/articles/989957.pdf
Data publikacji:
2017
Wydawca:
Instytut Medycyny Wsi
Tematy:
menopause
spine pain
ndi
odi
Opis:
Introduction and objective. Postmenopausal women working in agriculture suffer from spinal pain for two overlapping reasons, the first is related to the menopause and the second to the specificity of rural work, which includes lifting heavy objects and changing weather conditions. Spinal pain affects the daily life of women as well as their ability to work. The objective of the study was to analyse the impact of spinal pain on activities of daily life in Polish postmenopausal women performing agricultural work. Materials and method. The study was conducted in 2016 in Poland and included 1,119 post-menopausal women living in rural areas and working in agriculture. The women assessed the severity of spinal pain in 3 sections: neck, thorax and lumbar. Neck Disability Index (NDI) and Oswestry Low Back Disability Index (ODI) questionnaires were used to assess the impact of spinal pain on daily life activities. Generalized linear models were estimated in statistical analyses. Results. Postmenopausal women working in agriculture suffered most often from pain in the lumbar spine, less frequently in the neck, and the least in the thoracic. The most common was an isolated pain in only one section of the spine. Spinal pain disturbed the most the women’s rest, standing, lifting objects, while sleep, concentration, and walking the least. The impact of spinal pain on the activities of daily life, on average, was moderate, and increased with greater pain severity, the earlier the age the pain started, the higher the body weight, the lower education level and if there was a co-existing pain in any of the other spine sections. The impact of spinal pain on daily life activities did not depend on age between 45–65, WHR, age at last menstruation, parity, and number and types of births. Conclusions. The impact of spinal pain on daily life activities in postmenopausal women working in agriculture was assessed as moderate, on average, and depended mainly on spinal pain-related characteristics, such as severity, age at onset and co-existence of pain in any other spinal sections.
Źródło:
Annals of Agricultural and Environmental Medicine; 2017, 24, 1
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Air pollution and semen parameters in men seeking fertility treatment for the first time
Autorzy:
Wdowiak, Artur
Wdowiak, Edyta
Bień, Agnieszka
Bojar, Iwona
Iwanowicz-Palus, Grażyna
Raczkiewicz, Dorota
Powiązania:
https://bibliotekanauki.pl/articles/2161969.pdf
Data publikacji:
2019-06-14
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
air pollution
reproductive health
male infertility
semen analysis
sperm
sperm quality
Opis:
Objectives The purpose of this paper was to analyze the relationship between the selected chemical air pollutants found in the Lublin Province and the semen parameters of men seeking fertility treatment for the first time. Material and Methods The study involved an analysis of semen sample test results obtained from male patients first reporting for fertility treatment in reproductive health centers in the Lublin Province, Poland. The data set comprises semen parameters of 13 148 men, and the number of samples in the reference period was 255–769 annually. Data on air pollution were obtained from the website of the Polish General Environmental Inspectorate and included selected chemical air pollutant levels, i.e., NO₂, SO₂, O₃, and PM₁₀. Results The mean PM₁₀ levels in the air increased, on average, by 0.65 μg/m₃ annually in 2000–2015 (p = 0.029); the mean levels of O₃, NO₂, SO₂ did not change significantly in the analyzed period. There were increasing trends in the mean sperm density and total sperm count of the subjects in 1992–2015 (p < 0.001). The mean percentage of sperm with normal morphology significantly decreased in the subsequent analysis periods: 1992–1998 (p = 0.001); 1999–2009 (p < 0.001); 2010–2015 (p = 0.001). A significant negative correlation was found between the ozone levels in the air in the Lublin Province and the percentage of sperm with normal morphology (r = –0.8311, p = 0.040). Conclusions Exposure to ozone in the air contributes to decreased percentages of sperm with normal sperm morphology. Over the years, there was an increasing trend in sperm density in the men first reporting for fertility treatment, and a decreasing trend in the percentage of sperm with normal morphology. Int J Occup Med Environ Health. 2019;32(3):387–99
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2019, 32, 3; 387-399
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Social costs of loss in productivity-related absenteeism in Poland
Autorzy:
Genowska, Agnieszka
Fryc, Justyna
Pinkas, Jarosław
Jamiołkowski, Jacek
Szafraniec, Krystyna
Szpak, Andrzej
Bojar, Iwona
Powiązania:
https://bibliotekanauki.pl/articles/2167461.pdf
Data publikacji:
2017-10-06
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
sickness absence
indirect costs
productivity loss
occupational medicine
working age
inequalities in health
Opis:
Objectives The aim of this study was to estimate indirect costs associated with losses in productivity due to sickness absence among registered workers in Poland. Material and Methods Data on sick leave durations in 2013 was obtained from the Social Insurance Institution (SII) (Zakład Ubezpieczeń Społecznych – ZUS). Based on the number of assumptions, this data was used for calculating absence durations. The costs of lost productivity were estimated on the basis of the measure of gross value added. Results Estimated losses in productivity due to absenteeism in 2013 together accounted for 4.33% of gross domestic product (GDP) (17.09 billion euro). In the female population, the total value of losses amounted to 9.66 billion euro, but excluding the costs of pregnancy, childbirth, and puerperium (2.96 billion euro), it was 6.7 billion euro. In the male population, the loss amounted to 7.43 billion euro. The highest overall costs of sickness absence based on age were found in the age group of 30–39 years (5.14 billion euro, including pregnancy, childbirth, and puerperium – 1.474 billion euro; respiratory diseases – 0.632 billion euro, injuries and poisonings – 0.62 billion euro). In the group of people aged > 40 years, the highest cost was generated by bone-muscular diseases (1.553 billion euro) and injuries and poisoning (1.251 billion euro). Higher losses in the productivity of women in addition to pregnancy, childbirth, and puerperium were due to mental and behavioral disorders (0.71 billion euro), diseases of the genitourinary system (0.38 billion euro), and neoplasms (0.35 billion euro). At the same time, in men, compared to women, we observed higher losses due to injuries and poisoning (1.65 billion euro), and diseases of musculoskeletal (1.26 billion euro), nervous (0.79 billion euro), circulatory (0.65 billion euro), and digestive (0.41 billion euro) systems. Conclusions Improvement and further development of effective strategies for prevention of complications of pregnancy and chronic diseases in the workplace are necessary. Policies aimed at reduction of sickness absence could potentially increase prosperity and the socioeconomic situation in Poland. Int J Occup Med Environ Health 2017;30(6):917–932
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2017, 30, 6; 917-932
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Intensification of menopausal symptoms among female inhabitants of East European countries
Autorzy:
Bojar, Iwona
Lyubinets, Oleh
Novotny, Jozef
Stanchak, Yaroslav
Tiszczenko, Evgenii
Owoc, Alfred
Raczkiewicz, Dorota
Powiązania:
https://bibliotekanauki.pl/articles/989061.pdf
Data publikacji:
2016
Wydawca:
Instytut Medycyny Wsi
Tematy:
menopause
menopausal symptoms
eastern europe
Opis:
The objective of the study was analysis of the occurrence and intensity of menopausal symptoms in postmenopausal women from Poland, Belarus, Ukraine, Czech Republic, Slovakia and Poland. The study was conducted during the period 2014–2015 among postmenopausal women living in the areas of Poland, Belarus, Ukraine, Czech Republic and Slovakia. The degree of menopausal complaints was assessed using the Kupperman Menopausal Index and Greene Climacteric Scale. The respondents were additionally asked about age, educational level, place of residence, marital status and age at last menstrual period. Into the study were enrolled women aged 50–65, minimum 2 years after the last menstrual period, who had a generally good state of health and did not use hormone replacement therapy. The results were subjected to statistical analysis. The intensity of all menopausal symptoms measured by the Kupperman Menopausal Index and Greene Climacteric Scale was similar in Poland, Czech Republic and Slovakia. In these countries, severe, moderate and mild menopausal symptoms measured by Kupperman Menopausal Index occurred with a similar frequency. Similar results were also obtained in the subscales of psychological, somatic and vasomotor symptoms according to the Greene Climacteric Scale. Nearly a half of the women from Belarus did not report symptoms measured by Kupperman Menopausal Index. They obtained significantly lower menopausal complaints in the subscales of psychological and somatic symptoms according to the Greene Climacteric Scale, compared to the inhabitants of the remaining countries. The majority of women from the Ukraine had mild menopausal symptoms as measured by the Kupperman Menopausal Index. They had significantly more severe complaints in the subscales of psychological, somatic and vasomotor symptoms according to the Greene Climacteric Scale, compared to the inhabitants of the remaining countries in the study. The intensity of menopausal symptoms in women from Ukraine and Belarus was related with educational level, place of residence, and marital status, whereas in women from Poland, Czech Republic and Slovakia, only with marital status.
Źródło:
Annals of Agricultural and Environmental Medicine; 2016, 23, 3
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Stress at the place of work and cognitive functions among women performing intellectual work during peri- and post-menopausal period
Autorzy:
Gujski, Mariusz
Pinkas, Jarosław
Juńczyk, Tomasz
Pawełczak-Barszczowska, Adrianna
Raczkiewicz, Dorota
Owoc, Alfred
Bojar, Iwona
Powiązania:
https://bibliotekanauki.pl/articles/2161846.pdf
Data publikacji:
2017-10-06
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
Cognitive function
menopause
stress at work
woman health
CNS vital signs
intellectual work
Opis:
Objectives The analysis of the relationship between stress at work and results of cognitive functions amongst women, at peri- and post-menopausal age, performing intellectual work. Material and Methods The study group included women, aged 45–66 years old, employed as intellectual workers. Research instruments were: the Montreal Cognitive Assessment; computer tests of the CNS Vital Signs; the Subjective Work Characteristics Questionnaire, and a questionnaire designed by the author. The results were statistically analyzed. Results Nearly a half of respondents experienced high stress at the place of work; 1/3 – on the average level, on a low level – every fifth. The largest number of respondents experienced stress caused by social contacts. Among a half of the women, stress was caused by the lack of awards at work, followed by the lack of support. Slightly fewer of them experienced stress caused by the feeling of psychological load related to the complexity of work or the feeling of uncertainty caused by the organization of work. Every third woman experienced stress due to the sense of responsibility or the lack of control. The smallest number experienced stress caused by physical arduousness, the sense of threat and unpleasant working conditions. The examined women obtained the best results with respect to simple attention, the worst results – with respect to the reaction time. The results concerning the remaining 9 cognitive functions were ranked in the middle of the aforementioned results. The intensity of stress at work and factors which caused this stress, negatively correlated with simple attention of women in the early peri-menopausal period, while positively correlating with the psychomotor and processing speed of women in the late peri-menopausal period. Among the post-menopausal women, negative correlations were observed between the majority of cognitive functions and the intensity of stress at work, and the majority of factors which caused this stress. Conclusions Cognitive functions of the examined women remained within the range of average evaluations, and were correlated with stress-inducing factors at the place of work. Int J Occup Med Environ Health 2017;30(6):943–961
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2017, 30, 6; 943-961
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparison of the efficiency of two commercial kits - ELFA and Western blot in estimating the phase of Toxoplasma gondii infection in pregnant women
Autorzy:
Sroka, Jacek
Wójcik-Fatla, Angelina
Zając, Violetta
Sawczyn, Anna
Cisak, Ewa
Karamon, Jacek
Dutkiewicz, Jacek
Bojar, Iwona
Powiązania:
https://bibliotekanauki.pl/articles/991156.pdf
Data publikacji:
2016
Wydawca:
Instytut Medycyny Wsi
Tematy:
toxoplasmosis
pregnant women
seroprevalence
antibodies
igg
igm
elfa
western blot
avidity
correlation of serologic tests
Opis:
Sera of 89 pregnant women were selected according to the results of ELFA IgM, IgG and avidity IgG, and tested with commercial tests IgM, IgG and avidity IgG Western Blot (WB) to compare the efficacy of both techniques in determining the phase of T. gondii infection. In total, 81 of 89 tested sera (91.0%) were classified as positive, both in the ELFA and WB tests for the presence of anti-Toxoplasma antibodies of class IgG, indicating a past infection, while the prevalence of anti-Toxoplasma positive reactions associated with the antibodies of class IgM indicating a recent infection was much lower – 31.5% and 20.2%, respectively. Sera of 81 women were also tested in the ELFA and WB tests for avidity, e.g. ability of forming high-molecular IgG antibody complexes. Low or medium results in these tests (in this study all classified as low), indicating a recent infection, were detected by ELFA and WB in 22.2% and 45.7% of the total examined samples, respectively. The Spearman’s rank test for correlation, performed for recognition of quantitative data of the ELFA and WB tests (index, units or points), revealed a highly significant correlation between the ELFA and WB tests for homologous classes of antibodies, both for IgM and IgG (p<0.00001). In contrast, the ELFA and WB tests for detection of anti-Toxoplasma IgM antibodies were not correlated with the ELFA and WB tests for detection of anti-Toxoplasma IgG antibodies (p>0.05), except for the WB test for IgM antibodies, which showed a significant correlation with the ELFA test for IgG antibodies (p<0.01). A highly significant negative correlation between the ELFA and WB test for IgM antibodies and ELFA and WB tests for IgG avidity was demonstrated (p<0.01), except for a relationship between the WB test for IgM and WB for avidity, which was not significant. Such negative correlations are theoretically expected, as strong complexes with the participation of IgG antibodies are absent in the early phase of toxoplasmosis when early antibodies of IgM class are present. Summarizing, this study indicates the high usefulness of the commercial ELFA and WB tests in serodiagnostics of toxoplasmosis in pregnant women. Special attention should be paid to parallel detection of IgM antibodies and low values in the ELFA and WB tests for IgG avidity, which indicates a recent infection which may be associated with a clinical form of congenital toxoplasmosis and damage to the foetus.
Źródło:
Annals of Agricultural and Environmental Medicine; 2016, 23, 4
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-15 z 15

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