Autor: Bartosiewicz, Henryk. - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Polityka Związku Sowieckiego wobec państw Europy Środkowo-Wschodniej w latach 1944-1948
Autorzy:: Bartosiewicz, Henryk.
Współwytwórcy:: (gr.m). Recenzja
Data publikacji:: 1999
Wydawca:: Warszawa
Dostawca treści:: Bibliografia CBW

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Skocz do pozycji: 3.

Tytuł:: Distinct expression, localization and function of two Rab7 proteins encoded by paralogous genes in a free-living model eukaryote
Autorzy:: Osińska, Magdalena
Wiejak, Jolanta
Wypych, Emilia
Bilski, Henryk
Bartosiewicz, Rafał
Wyroba, Elżbieta
Powiązania:: https://bibliotekanauki.pl/articles/1039860.pdf
Data publikacji:: 2011
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: glycosylation
antipeptide antibodies
Real-Time PCR
Rab7 isotypes
RNAi
2D electrophoresis
Paramecium octaurelia
STED
Opis:: Rab7 GTPases are involved in membrane trafficking in the late endosomal/lysosomal pathway. In Paramecium octaurelia Rab7a and Rab7b are encoded by paralogous genes. Antipeptide antibodies generated against divergent C-termini recognize Rab7a of 22.5 kDa and Rab7b of 25 kDa, respectively. In 2D gel electrophoresis two immunoreactive spots were identified for Rab7b at pI about 6.34 and about 6.18 and only one spot for Rab7a of pI about 6.34 suggesting post-translational modification of Rab7b. Mass spectrometry revealed eight identical phosphorylated residues in the both proteins. ProQ Emerald staining and ConA overlay of immunoprecipitated Rab7b indicated its putative glycosylation that was further supported by a faster electrophoretic mobility of this protein upon deglycosylation. Such a post-translational modification and substitution of Ala140 in Rab7a for Ser140 in Rab7b may result in distinct targeting to the oral apparatus where Rab7b associates with the microtubular structures as revealed by STED confocal and electron microscopy. Rab7a was mapped to phagosomal compartment. Absolute qReal-Time PCR analysis revealed that expression of Rab7a was 2.6-fold higher than that of Rab7b. Upon latex internalization it was further 2-fold increased for Rab7a and only slightly for Rab7b. Post-transcriptional gene silencing of rab7a suppressed phagosome formation by 70 % and impaired their acidification. Ultrastructural analysis with double immunogold labeling revealed that this effect was due to the lack of V-ATPase recruitment to phagolysosomes. No significant phenotype changes were noticed in cells upon rab7b silencing. In conclusion, Rab7b acquired a new function, whereas Rab7a can be assigned to the phagolysosomal pathway.
Źródło:: Acta Biochimica Polonica; 2011, 58, 4; 597-607
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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