- Tytuł:
- Cryptosporidium parvum – zoonotic subtype IIdA15G1 in a Slovakian patient
- Autorzy:
-
Mravcova, K.
Strkolcova, G.
Mucha, R.
Barbusinova, E.
Goldova, M.
Kacirova, J.
Madar, M. - Powiązania:
- https://bibliotekanauki.pl/articles/2085903.pdf
- Data publikacji:
- 2020
- Wydawca:
- Instytut Medycyny Wsi
- Tematy:
-
Slovakia
nested PCR
Cryptosporidium spp
zoonotic subtype
veterinary student - Opis:
- Introduction and objectives. The parasite Cryptosporidium spp. is an intracellular protozoa which has a broad range of hosts and zoonotic potential. It presents a serious health risk for agricultural workers and veterinarians. The aim of the study was to identify the species and subtypes of Cryptosporidium occurring in a veterinary student who came into contact with calves on a farm. Materials and method. The Ziehl-Neelsen staining technique was employed to confirm the presence of Cryptosporidium oocysts. ELISA test was applied to detect coproantigen in faecal specimens. Nested PCR was used to amplify a small ribosomal subunit (SSU rRNA) and sequencing of the GP60 gene served to identify the zoonotic subtypes. Results. The nested PCR allowed to confirm the C. parvum species; subsequently, the IIdA15G1 zoonotic subtype was identified. Conclusion. This is the first confirmed case in Slovakia of human cryptosporidiosis caused by the unique subtype IIdA15G1.
- Źródło:
-
Annals of Agricultural and Environmental Medicine; 2020, 27, 3; 485-488
1232-1966 - Pojawia się w:
- Annals of Agricultural and Environmental Medicine
- Dostawca treści:
- Biblioteka Nauki
Artykuł