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    Wyświetlanie 1-10 z 10
    Tytuł:
    Equine herpesvirus type 1 affects mitochondrial network morphology and reactive oxygen species generation in equine dermal cell line
    Autorzy:
    Bartak, M.
    Chodkowski, M.
    Słońska, A.
    Bańbura, M.W.
    Cymerys, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087318.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    equine herpesvirus type 1
    equine dermal cell line
    mitochondria
    reactive oxygen species
    Źródło:
    Polish Journal of Veterinary Sciences; 2020, 23, 2; 309-312
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Application of NucleoCounter for the comprehensive assessment of murine cultured neurons during infection with Equine Herpesvirus type 1 (EHV-1)
    Autorzy:
    Chodkowski, M.
    Serafińska, I.
    Brzezicka, J.
    Bańbura, M.W.
    Cymerys, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087859.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    NucleoCounter NC-3000
    viability
    cell cycle
    apoptosis
    neurons
    EHV-1
    Źródło:
    Polish Journal of Veterinary Sciences; 2017, 4; 831-834
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    First report of the oncolytic effect of EHV-1 on the non-small lung cancer – in vitro studies
    Autorzy:
    Chodkowski, M.
    Słońska, A.
    Bartak, M.
    Bańbura, M.W.
    Cymerys, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087133.pdf
    Data publikacji:
    2021
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    EHV-1
    adenocarcinoma cell line
    oncolytic virus
    Źródło:
    Polish Journal of Veterinary Sciences; 2021, 24, 4; 607-610
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Equine herpesvirus type 1 (EHV-1) replication in primary murine neurons culture
    Autorzy:
    Cymerys, J.
    Dzieciatkowski, T.
    Slonska, A.
    Bierla, J.
    Tucholska, A.
    Chmielewska, A.
    Golke, A.
    Banbura, M.W.
    Powiązania:
    https://bibliotekanauki.pl/articles/31974.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    replication
    animal disease
    horse
    equine herpesvirus 1
    respiratory tract infection
    neurological disorder
    abortion
    neuron
    Opis:
    Equine herpesvirus-1 (EHV-1) infections cause significant economic losses for equine industries worldwide as a result of abortion, respiratory illness, and neurologic disease in all breeds of horses. The occurrence of abortions caused by EHV-1 has repeatedly been confirmed in Poland, but neurological manifestations of the infection have not been described yet. Also it is unknown how the infection of neurons with non-neuropathogenic strains is regulated. To further understand the virus- neuron interaction we studied two strains of EHV-1 in murine primary neuron cell cultures. Both strains were isolated from aborted fetuses: Rac-H, a reference strain isolated by Woyciechowska in 1959 (Woyciechowska 1960) and Jan-E isolated by Bańbura et al. (Bańbura et al. 2000). Upon infection of primary murine neuronal cell cultures with Jan-E or Rac-H strains, a cytopathic effect was observed, manifested by a changed morphology and disintegration of the cell monolayer. Positive results of immunofluorescence, nPCR and real-time PCR tests indicated high virus concentration in neurons, meaning that both EHV-1 strains were likely to replicate in mouse neurons in vitro without the need for adaptation. Moreover, we demonstrated that some neurons may survive (limited) virus replication during primary infection, and these neurons (eight weeks p.i.) harbour EHV-1 and were still able to transmit infection to other cells.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Replication kinetics of neuropathogenic and non-neuropathogenic equine herpesvirus type 1 (EHV-1) strains in primary murine neurons and ED cell line
    Autorzy:
    Cymerys, J.
    Slonska, A.
    Brzezicka, J.
    Tucholska, A.
    Chmielewska, A.
    Rola, J.
    Malik, P.
    Banbura, M.W.
    Powiązania:
    https://bibliotekanauki.pl/articles/32263.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Equine herpesvirus type 1 (EHV-1) causes respiratory infections, abortion and neurological disorders in horses. Molecular epidemiology studies have demonstrated that a single-point mutation in DNA polymerase gene, resulting in an amino acid variation (N752/D752), is significantly associated with the neuropathogenic potential of EHV-1 strains. The aim of the study was to elucidate if there are any differences between neuropathogenic (EHV-1 26) and non-neuropathogenic (Jan-E and Rac-H) EHV-1 strains in their ability to infect neuronal cells. For the tested EHV-1 strains, cytopathic effect (CPE) was manifested by changed morphology of cells, destruction of actin cytoskeleton and nuclei degeneration, which led to focal degeneration. Moreover, EHV-1 26 strain caused fusion of the infected cells to form syncytia in culture. Real-time PCR analysis demonstrated that both neuropathogenic and non-neuropathogenic EHV-1 strains replicated in neurons and ED cells (equine dermal cell line) at a similar level. We can assume that a point mutation in the EHV-1 polymerase does not affect viral replication in this cell type.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Equid herpesvirus type 1 (EHV-1) disrupts actin cytoskeleton during productive infection in equine leukocytes
    Autorzy:
    Drebert, Z.
    Golke, A.
    Cymerys, J.
    Slonska, A.
    Chmielewska, A.
    Tucholska, A.
    Banbura, M.W.
    Powiązania:
    https://bibliotekanauki.pl/articles/30687.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Equid herpesvirus type 1 (EHV-1) is a prevalent causative agent of equine diseases worldwide. After primary replication in the respiratory epithelium the virus disseminates systemically through a peripheral blood mononuclear cell (PBMC)-associated viraemia. EHV-1 is the only alphaherpes-virus known so far which is capable of establishing latent infection not only in neurons but also in immune system cells (mainly in lymphocytes and macrophages). Since leukocytes are not the target cells for viral replication but are used to transport EHV-1 to the internal organs, the question remains how the virus avoids the immune response and whether it could potentially be associated with virus-induced cytoskeletal rearrangements. Therefore, the aim of this study was to investigate the progress of EHV-1 replication in leukocytes stimulated by phytohemagglutinin and the impact of EHV-1 infection on the actin cytoskeleton. Using the real-time PCR method we evaluated the quantity of viral DNA from samples collected at indicated time points post infection. In order to examine possible changes in actin cytoskeleton organization due to EHV-1 infection, we performed immunofluorescent staining using TRITC-phalloidin conjugate. The results showed that EHV-1 replicates in leukocytes at a restricted level but with the accompaniment of chromatin degradation. Simultaneously, infection with EHV-1 caused disruption of the actin cytoskeleton; this was particularly apparent in further stages of infection. Disruption of the actin cytoskeleton may lead to the limited release of the virus from the cells, but may be also beneficial for the virus, since at the same time it potentially impairs the immune function of leukocytes.
    Źródło:
    Polish Journal of Veterinary Sciences; 2015, 18, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The xCELLigence system for real-time and label-free analysis of neuronal and dermal cell response to Equine Herpesvirus type 1 infection
    Autorzy:
    Golke, A.
    Cymerys, J.
    Slonska, A.
    Dzieciatkowski, T.
    Chmielewska, A.
    Tucholska, A.
    Banbura, M.W.
    Powiązania:
    https://bibliotekanauki.pl/articles/30665.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    xCELLigence system
    real-time analysis
    label-free analysis
    neuronal cell response
    dermal cell response
    cell response
    equine herpesvirus 1
    animal infection
    herpesvirus
    neuron
    neuronal cell
    dermal cell
    Opis:
    Real-time cell electronic sensing (RT-CES) based on impedance measurements is an emerging technology for analyzing the status of cells in vitro. It allows label-free, real time monitoring of the biological status of cells. The present study was designed to assess dynamic data on the cell processes during equine herpesvirus type 1 (EHV-1) infection of ED (equine dermal) cells and primary murine neuronal cell culture. We have demonstrated that the xCELLigence system with dynamic monitoring can be used as a rapid diagnostic tool both to analyze cellular behavior and to investigate the effect of viral infection.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    “Interactive Power Devices for Efficiency in Automotive with Increased Reliability and Safety” – the European Project Concerning the Main Automotive and Transport Application Domains
    Autorzy:
    Kadlec, J.
    Kuchta, R.
    Novotny, R.
    Hajduga, A.
    Sekrecki, M.
    Roszczyk, P.
    Dybowski, J.
    Kalbarczyk, J.
    Zieliński, W.
    Bańbura, P.
    Burliński, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/263013.pdf
    Data publikacji:
    2015
    Wydawca:
    Sieć Badawcza Łukasiewicz. Przemysłowy Instytut Motoryzacji
    Tematy:
    electric vehicle
    NAND flash memory
    reliability
    EV powertrain
    electric drive efficiency
    pojazd elektryczny
    pamięć flash NAND
    niezawodność
    napęd
    napęd elektryczny
    efektywność
    Opis:
    The overall objective of the IDEAS project is to develop advanced packaging for power supply components and new generation memory systems applicable to Electric and ICE propelled vehicles, with paying attention to considering also the aspects that have not been addressed yet in the running ENIAC and ARTEMIS Automotive projects. A major challenge related to electronic devices in the automotive applications is the reliability of power supply systems which must be capable to assure the functionality of subsystems in all operating conditions, inclusive of ageing. The control systems, which rely on multicore microcontrollers and complex software architectures, require increasingly stringent constraints from the memory devices which need to be designed for very high bandwidth, speed and reliability. In the following thesis, a few aspects of the IDEAS project will be presented: a review of important factors that affect the reliability and life-cycle endurance of NAND flash memories, multispeed gear application in electric drives and its influence on the energy efficiency. The problem of electromagnetic compatibility of electronic devices will also be dealt with in the paper
    Źródło:
    Archiwum Motoryzacji; 2015, 69, 3; 31-64
    1234-754X
    2084-476X
    Pojawia się w:
    Archiwum Motoryzacji
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    HybProbes-based real-time PCR assay for rapid detection of equine herpesvirus type 2 DNA
    Autorzy:
    Osinska, E.
    Golke, A.
    Slonska, A.
    Cymerys, J.
    Banbura, M.W.
    Dzieciatkowski, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/30252.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    rapid detection
    equine herpesvirus
    real-time polymerase chain reaction
    horse
    herpesvirus
    Gammaherpesvirinae
    Rhadinovirus
    veterinary virology
    Opis:
    Equid herpesvirus type 2 (EHV-2) together with equid herpesvirus type 5 are members of Gammaherpesvirinae subfamily, genus Rhadinovirus. EHV-2 is one of major agents causing diseases of horses common worldwide. A possible role of EHV-2 in reactivating latent equid herpesvirus type-1 has been suggested, because reactivation of latent EHV-1 was always accompanied by EHV-2 replication. Variety techniques, including cell culture, PCR and its modifications, have been used to diagnose EHV-2 infections. The aim of this study was to develop, optimize and determine specificity of real-time PCR (qPCR) for EHV-2 DNA detection using HybProbesR chemistry and to evaluate clinical samples with this method. The analytical sensitivity of assay was tested using serial dilutions of viral DNA in range between 70 and 7x105 copies/ml. The limit of detection (LOD) was calculated using probit analysis and was determined as 56 copies/ml. In further studies 20 different clinical samples were tested for the presence of EHV-2. Described in-house qPCR method detected viral DNA in 5 of 20 specimens used. The results of this work show that developed HybProbes-based real-time PCR assay is very reliable and valuable for detection and quantification of equid herpesvirus type 2 DNA in different clinical samples. The high level of sensitivity, accuracy and rapidity provided by the LightCycler 2.0 instrument are favorable for the use of this system in the detection of EHV-2 DNA in veterinary virology.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influence of importin α/β and exportin 1 on equine herpesvirus type 1 (EHV-1) replication in primary murine neurons
    Autorzy:
    Slonska, A.
    Cymerys, J.
    Skwarska, J.
    Golke, A.
    Banbura, M W
    Powiązania:
    https://bibliotekanauki.pl/articles/31471.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Viruses replicating in the nucleus need to cross the nuclear membrane barrier during infection, therefore disruption of specific nuclear transport pathways is crucial for their replication cycle. In the present study we have investigated the influence of nucleo-cytoplasmic transport inhibitors – ivermectin and leptomycin B, on EHV-1 replication in primary murine neurons. Obtained results suggest that the examined proteins – exportin 1 and importin α/β may participate, but are not required, during EHV-1 infection. Based on these results, it can be assumed that EHV-1 is able to use other receptors for nucleo-cytoplasmic transport.
    Źródło:
    Polish Journal of Veterinary Sciences; 2013, 16, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-10 z 10

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