Autor: Bakhsh, M. - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Identification of viruses infecting cucurbits and determination of genetic diversity of cucumber mosaic virus in Lorestan province, Iran
Autorzy:: Hasanvand, V.
Shams-bakhsh, M.
Powiązania:: https://bibliotekanauki.pl/articles/66238.pdf
Data publikacji:: 2017
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: identification
virus
cucurbit
determination
genetic diversity
cucumber mosaic virus
RFLP analysis
Lorestan province
Iran
Opis:: Various viral pathogens infect Cucurbitaceae and cause economic losses. Th e aim of the present study was to detect plant viral pathogens including Cucumber mosaic virus (CMV), Cucumber green mottle mosaic virus (CGMMV), Zucchini yellow mosaic virus (ZYMV), Cucurbit yellow stunting disorder virus (CYSDV) and Cucurbit chlorotic yellows virus (CCYV) in Lorestan province, in western Iran, and also to determine CMV genetic diversity in Iranian populations. A total of 569 symptomatic leaf samples were collected in 2013 and 2014 from cucurbits growing regions in Lorestan province. Th e collected samples were assessed for viral diseases by ELISA. Th e results showed virus incidences in most regions. Th en, the infection of 40 samples to CMV was confi rmed by RT-PCR. Moreover, to distinguish between the two groups (I and II) of CMV, PCR products were digested by two restriction enzymes XhoI and EcoRI. Results of the digestion showed that the isolates of Lorestan belonged to group I. Th e CMV-coat protein gene of eight isolates from diff erent regions and hosts was sequenced and phylogenetic analysis was performed. Subsequent analyses showed even more genetic variation among Lorestan isolates. Th e phylogenetic tree revealed that Lorestan province isolates belonged to two IA and IB subgroups and could be classifi ed together with East Azerbaijan province isolates. Th e results of the present study indicate a wide distribution of CMV, ZYMV, CGMMV, CYSDV and CCYV viruses in cucurbits fi elds of Lorestan province and for the fi rst time subgroup IB of CMV was reported on melon from Iran.
Źródło:: Journal of Plant Protection Research; 2017, 57, 2
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Prevalence and phylogenetic analysis of Fig mosaic virus and Fig badnavirus-1 in Iran
Autorzy:: Alimoradian, M.
Rakhshandehroo, F.
Shams-bakhsh, M.
Powiązania:: https://bibliotekanauki.pl/articles/66369.pdf
Data publikacji:: 2016
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: prevalence
phylogenetic analysis
Fig mosaic virus
Fig badnavirus-1
Iran
fig tree
plant disease
Opis:: Fig mosaic virus (FMV) and Fig badnavirus-1 (FBV-1) are two of the most important fig infecting viruses. The incidence and distribution of FBV-1 and FMV were determined by testing in PCR 138 asymptomatic and symptomatic samples. These samples were collected from 60 fig gardens and agricultural fields in three provinces of Iran. The fig infecting viruses FBV-1 and FMV, respectively, were detected in 92 (66.6%) and 34 (24.6%) samples collected from all the surveyed fields. Overall, 24 out of 138 (17.3%) samples showed mixed infections. The sequence analysis of a genomic fragment of 922 nt, comprising the entire ORF-2 and part of the 5’ termini of the ORF-3 of 10 selected FBV-1 Iranian isolates from different provinces, and of the type member from GenBank (Acc. No: JF411989), showed a variation ranging from 1 to 3% at nucleotide level and 1% at the amino acid level. The phylogenetic analysis grouped the FBV-1 isolates into two groups, with the Iranian isolates clustered in two distinct subgroups of group I, according to their geographical origin. In our research, the prevalence and sequence analysis of FBV-1 as the only identified DNA virus infecting fig trees, was studied for the first time in Iran.
Źródło:: Journal of Plant Protection Research; 2016, 56, 2
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Genetic diversity among Xanthomonas citri subsp. citri strains in Iran
Autorzy:: Rezaei, M.K.
Shams-Bakhsh, M.
Alizadeh, A.
Powiązania:: https://bibliotekanauki.pl/articles/65267.pdf
Data publikacji:: 2012
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:: Citrus bacterial canker (CBC) is one of the most important diseases of citrus. It is caused by Xanthomonas citri subsp. citri (Xcc). To investigate the variability of Xcc, a collection of twenty-five strains were isolated from the Fars, Hormozgan, Kerman and Sistan-va-Baluchestan provinces of Iran. The twenty-five strains were assessed phenotypically and genetically. These strains had similar biochemical properties. Based on host range determination, the strains were divided into two groups; the first group was pathogenic on Mexican lime (Citrus aurantifolia), citrumelo (Poncirus trifoliata x C. paradisi), citrange (C. sinensis x P. trifoliata) and sour orange (C. aurantium) varieties. The second group was pathogenic on Mexican lime only. Profile of cellular soluble proteins analyzed by sodium dodecyl sulphate-polyacryamid gel electrophoresis (SDS-PAGE) did not reveal any considerable differences among strains. Genetic diversity analyses were performed using two marker systems; repetitive polymerase chain reaction (rep-PCR) and random amplified polymorphic DNA (RAPD). The results of this research showed that two primers, ERIC 1R and 232, with the highest marker index, resulted in the most genetic variability among strains. Cluster analysis by band patterns showed that strains from the Sistan-va-Baluchestan province were a different group, so it was concluded that geographical origin of strains from the Sistan-va-Baluchestan province is different than the geographical origin of strains isolated from other provinces.
Źródło:: Journal of Plant Protection Research; 2012, 52, 1
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Molecular detection and therapeutic study of Trypanosoma brucei evansi from naturally infected horses in Punjab, Pakistan
Autorzy:: Zahoor, J.
Kashif, M.
Nasir, A.
Bakhsh, M.
Qamar, M.F.
Sikandar, A.
Rehman, A.
Powiązania:: https://bibliotekanauki.pl/articles/16539325.pdf
Data publikacji:: 2022
Wydawca:: Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:: equines
prevalence
Trypanosome brucei evansi
therapeutic trial
horses
Opis:: Trypanosomiasis is one of the severe pathogenic infections, caused by several Trypanosoma species, affecting both animals and humans, causing substantial economic losses and severe illness. The objective of this study was to determine the molecular diagnosis and the risk factors associated with trypanosomiasis in District Jhang, Punjab, Pakistan. For this purpose, blood samples were randomly collected from 200 horses. A predesigned questionnaire was used to collect data on risk factors before the sample collection. The microscopy examination through Giemsa staining, formol gel test and PCR techniques were used to find the prevalence. The prevalence was recorded as 22.5% with microscopy examination, 21% through formol gel test and 15.5% with PCR based results. Analysis of risk factors associated with Trypanosoma brucei evansi occurrence was carried out using Chi-square test. It showed the prevalence of Trypanosoma brucei evansi was significantly (p<0.05) associated with sex, age, rearing purpose and body condition whereas non-significantly (p>0.05) with insects control practices. This study supports the idea that PCR is a sensitive, robust and more reliable technique to diagnose trypanosomiasis. It was concluded that Trypanosoma brucei evansi is widely prevalent in Jhang (Pakistan), highlighting a dire need to develop control strategies and education programmes to control this disease in developing countries.
Źródło:: Polish Journal of Veterinary Sciences; 2022, 25, 3; 429-435
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Comparison of the effect of zinc oxide nanoparticles and extract of Acorus calamus applied topically on surgical wounds inflicted on the skin of rabbits
Autorzy:: Abbas, A.
Muhammad, S.A.
Ashar, A.
Mehfooz, S.A.
Rauf, A.
Bakhsh, M.
Nadeem, T.
Fu, H.
Powiązania:: https://bibliotekanauki.pl/articles/16648000.pdf
Data publikacji:: 2023
Wydawca:: Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:: antibiotic resistance
nanoparticle
plant extract
wound
Opis:: Antibiotics are used for postsurgical wound healing purposes but unfortunately, resistance against them demands some alternatives for quick recovery. Sepsis of wounds is a challenge for medical as well as veterinary professionals. Nanoparticles have significant advantages in wound treatment and drug resistance reversal. This study was conducted to appreciate emerging alternates of antibiotics like zinc oxide nanoparticles and plant extracts in topical application. Zinc oxide is considered a good wound healer and its nanoparticles are easy to access. So, the efficacies of zinc oxide nanoparticles and sweet flag plant extract ointments were tested to compare modern and traditional therapeutics as sweet flag is considered a pure medicinal plant. Rabbits were selected for this study due to the healing properties of their skin. Wounds were inflicted on the thoracolumbar region and treated for 29 days post-surgically daily with normal saline and the ointment of zinc oxide nanoparticles and sweet flag extract ointment, prepared in a hydrophilic solvent. Wound shrinkage was observed daily and histopathological analysis was made and results were compared. Zinc oxide nanoparticles ointment showed the most satisfactory results for every parameter included in the study. No side effects of its topical application were observed. Healing was normal without any complications. The preparations of zinc oxide nanoparticles may help in the era of antibiotic resistance as topical drugs in the future.
Źródło:: Polish Journal of Veterinary Sciences; 2023, 26, 2; 285-293
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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